公开(公告)号：US11459620B2

公开(公告)日：2022-10-04

申请号：US16706394

申请日：2019-12-06

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Yongmei (Alexis) Ji ,  Pius Brzoska ,  Angela Burrell ,  Craig Cummings ,  Catherine O'Connell

IPC: C12P19/34 ,  C12Q1/689 ,  C12Q1/6806

Abstract: Disclosed are compositions, assays, methods, diagnostic methods, kits and diagnostic kits for the specific and differential detection of Mycobacterium avium subsp. paratuberculosis from samples including veterinary samples, clinical samples, food samples, forensic sample, an environmental sample (e.g., soil, dirt, garbage, sewage, air, or water), including food processing and manufacturing surfaces, or a biological sample.

公开(公告)号：US09926609B2

公开(公告)日：2018-03-27

申请号：US14978243

申请日：2015-12-22

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Yongmei Ji ,  Pius Brzoska ,  Angela Burrell ,  Craig Cummings ,  Catherine O'Connell

IPC: C12P19/34 ,  C12Q1/68

CPC classification number: C12Q1/689 ,  C12Q1/6806 ,  C12Q2600/112 ,  C12Q2600/16

Abstract: Disclosed are compositions, assays, methods, diagnostic methods, kits and diagnostic kits for the specific and differential detection of Mycobacterium avium subsp. paratuberculosis from samples including veterinary samples, clinical samples, food samples, forensic sample, an environmental sample (e.g., soil, dirt, garbage, sewage, air, or water), including food processing and manufacturing surfaces, or a biological sample.

公开(公告)号：US09024002B2

公开(公告)日：2015-05-05

申请号：US13788715

申请日：2013-03-07

Applicant: Life Technologies Corporation

Inventor： Craig Cummings ,  Olga Petrauskene ,  Lily Wong ,  Allison De Los Reyes

IPC: C12Q1/68 ,  C12P19/34

CPC classification number: C12Q1/689 ,  C12Q2600/158 ,  C12Q2600/16 ,  Y02A50/451

Abstract: Described are compositions, methods and kits for detection and/or differential detection of serovars of Salmonella enterica subsp. enterica serovar such as S. Heidelberg and S. Hadar in samples. Some embodiments relate to multiplex amplification based molecular assays.

Abstract translation: 描述了用于检测和/或差异检测肠炎沙门氏菌血清型的组合物，方法和试剂盒。 肠溶血清病例如海德堡和S.Hadar在样品中。 一些实施方案涉及基于多重扩增的分子测定。

公开(公告)号：US20140272940A1

公开(公告)日：2014-09-18

申请号：US14206716

申请日：2014-03-12

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Wayne Ge ,  Craig Cummings ,  Darcy Myers ,  Quoc Hoang ,  Catherine O'Connell ,  Maxim Brevnov ,  Daniel Kephart

IPC: C12Q1/68

CPC classification number: C12Q1/686 ,  C12Q1/6809 ,  C12Q2525/155 ,  C12Q2525/161 ,  C12Q2525/197 ,  C12Q2531/113

Abstract: The present disclosure, in some embodiments, relates to compositions and methods for detection of multiple target nucleic acid sequences by a single nucleic acid amplification based assay (e.g. PCR). Compositions comprising two or more primers are described for multiple target nucleic acid detection. Methods for differential detection of microorganisms (including strains/serovars/subtypes thereof) and cell types comprise a single step method to detect a signature of target nucleic acid sequences comprising: two, three or more target nucleic acids that are uniquely present in a microorganism/strain/serovar/subtype and/or cell and absent in other closely related organisms/cells. Embodiments relate to methods of diagnosis of diseases or conditions that can be detected by detecting the presence of two or more nucleic acid target markers.

Abstract translation: 在一些实施方案中，本公开涉及通过基于单个核酸扩增的测定（例如PCR）检测多个靶核酸序列的组合物和方法。 描述了包含两个或多个引物的组合物用于多靶核酸检测。 用于差异检测微生物（包括菌株/血清型/亚型）和细胞类型的方法包括检测靶核酸序列的特征的单步方法，其包括：两个或三个以上唯一存在于微生物/ 菌株/血清型/亚型和/或细胞，并且在其他紧密相关的生物/细胞中不存在。 实施方案涉及通过检测两种或多种核酸靶标志物的存在可以检测的疾病或病症的诊断方法。