公开(公告)号：US20160228876A1

公开(公告)日：2016-08-11

申请号：US15017393

申请日：2016-02-05

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Yong Chu ,  Jeffrey Marks ,  Jacob Freudenthal ,  Mingsong Chen ,  Tiong Han Toh ,  Mauro Aguanno ,  Lik Seng Lau ,  Lian Seng Loh ,  Kok Siong Teo ,  Zeng Wei Chu ,  Xin Mathers ,  Michael Uy ,  Huei Yeo ,  Kuan Moon Boo ,  Way Xuang Lee ,  Chin Yong Koo ,  Wei Fuh Teo ,  Soo Yong Lau ,  Hon Siu Shin ,  Zeqi Tan ,  Thomas Wessel ,  Woo David

IPC: B01L7/00 ,  C12Q1/68 ,  G01N21/64

CPC classification number: B01L7/52 ,  B01L2200/145 ,  B01L2300/18 ,  B01L2300/1822 ,  C12Q1/6806 ,  G01N21/274 ,  G01N21/6452 ,  G01N21/6486 ,  G01N35/026 ,  G01N2021/6439 ,  G01N2035/00316 ,  G01N2035/00366 ,  G01N2201/061 ,  G01N2201/12

Abstract: A biological analysis system is provided. The system comprises a sample block assembly. The sample block assembly comprises a sample block configured to accommodate a sample holder, the sample holder configured to receive a plurality of samples. The system also comprises a control system configured to cycle the plurality of samples through a series of temperatures. The system further comprises an automated tray comprising a slide assembly, the tray configured to reversibly slide the sample block assembly from a closed to an open position to allow user access to the plurality of sample holders.

Abstract translation: 提供生物分析系统。 该系统包括样品块组件。 样品块组件包括被配置为容纳样品架的样品块，样品保持器被配置为接收多个样品。 该系统还包括配置成使多个样品循环通过一系列温度的控制系统。 所述系统还包括自动盘，其包括滑动组件，所述托盘被配置为可将所述样品块组件从关闭位置可逆地滑动至打开位置，以允许使用者接近所述多个样品保持器。

公开(公告)号：US20200003728A1

公开(公告)日：2020-01-02

申请号：US16486137

申请日：2018-02-16

Applicant: Life Technologies Corporation

Inventor： Nivedita Majumdar ,  Wallace George ,  Jeffrey Marks ,  Ming Jiang ,  Sylvia Chang ,  David Woo

IPC: G01N27/416 ,  G01N27/447

Abstract: Embodiments implementing selected automated quality control operations in sample processing instruments that analyze dye-labeled samples are disclosed. In some embodiments, temperature and/or pressure parameters are measured and compared to thresholds to determine whether warning should be provided and/or actions taken. Embodiments for implementing automated correction of spectral error during the instrument's normal runtime operation without requiring the user to conduct a special, separate calibration run are also disclosed.

公开(公告)号：US10147182B2

公开(公告)日：2018-12-04

申请号：US15350444

申请日：2016-11-14

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jacob Freudenthal ,  Jeffrey Marks ,  Francis Cheng ,  Thomas Wessel

IPC: G06K9/00 ,  G06T7/00 ,  G06T5/00 ,  G06T5/50

Abstract: A method for calibrating a biological instrument is provided. The method comprises the steps of acquiring an image of at least one biological sample array, determining a first region of interest within the image, wherein the first region of interest comprises a first plurality of locations on the at least one biological array; and identifying within the first region of interest, a plurality of image elements associated with each of the first plurality of locations on the at least one biological array.

公开(公告)号：US20180276849A1

公开(公告)日：2018-09-27

申请号：US15899308

申请日：2018-02-19

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Yong Chu ,  Jeffrey Marks

IPC: G06T7/73 ,  C12Q1/686 ,  G06K9/00 ,  G06T5/00 ,  G06T7/00 ,  G06T7/11 ,  G06T7/136 ,  G06T7/194

CPC classification number: G06T7/74 ,  C12Q1/686 ,  G06K9/00 ,  G06K2209/07 ,  G06T5/002 ,  G06T7/0012 ,  G06T7/11 ,  G06T7/136 ,  G06T7/194 ,  G06T2207/10064 ,  G06T2207/20024 ,  G06T2207/20208 ,  G06T2207/30072

Abstract: A method for analyzing biological reaction systems is provided. The method includes receiving an image of a substrate including a plurality of reaction sites after a biological reaction has taken place. Next, the method includes removing a noise background from the first image. The method includes determining an initial position of each reaction site based on an intensity threshold to generate a initial position set, then refining the initial position set of each reaction site based on an expected pattern of locations of the plurality of reaction sites to generate a first refined position set. The method further includes determining a presence or absence of a fluorescent emission from each reaction site based on the first refined position set and the first image.

公开(公告)号：US09809849B2

公开(公告)日：2017-11-07

申请号：US15016713

申请日：2016-02-05

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jeffrey Marks

IPC: G01N21/64 ,  C12Q1/68 ,  G01N21/27

CPC classification number: C12Q1/686 ,  G01N21/274 ,  G01N21/6428 ,  G01N21/6452 ,  G01N2021/6421 ,  G01N2201/04 ,  G01N2201/068 ,  G01N2201/12746

Abstract: The present teachings relate to a method and system for normalizing spectra across multiple instruments. In an embodiment of the present invention, the method comprises at least one reference instrument and a test instrument. Each instrument comprises at least one excitation filter and at least one emission filter arranged in pairs. Each instrument further comprises a pure dye plate comprising a plurality of wells. Each well contains a plurality of dyes where each dye comprises a fluorescent component. Fluorescent spectra are obtained from each instrument for each dye across multiple filter combinations to contribute to a pure dye matrix Mref for the reference instrument and pure dye matrix M for the test instrument. The pure dye spectra can then be multiplied by correction factors for each filter pair to result in corrected pure dye spectra, then normalized and the multicomponenting data can be extracted.