公开(公告)号：US20150218379A1

公开(公告)日：2015-08-06

申请号：US14402358

申请日：2013-03-14

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kyle Gee ,  Upinder Singh ,  Aleksey Rukavishnikov ,  Daniel Beacham ,  Shih-Jung Huang ,  Michael Janes ,  Wenjun Zhou

IPC: C09B11/24 ,  G01N33/58 ,  G01N33/50 ,  G01N33/84

CPC classification number: C09B11/24 ,  A61K49/0041 ,  C07D311/90 ,  G01N33/5005 ,  G01N33/582 ,  G01N33/84

Abstract: Disclosed herein are compounds, compositions, methods and kits for detecting pH in samples using pH-sensitive fluorescent dyes. The compounds disclosed herein are novel xanthene-derivative dyes comprising an aniline moiety with one or more electron donating groups, which dyes are for detecting pH in samples either in vitro or in vivo. Also described herein are processes for preparing said dyes for use in the disclosed compositions, methods and kits.

Abstract translation: 本文公开了使用pH敏感荧光染料检测样品中的pH的化合物，组合物，方法和试剂盒。 本文公开的化合物是包含具有一个或多个给电子基团的苯胺部分的新型呫吨衍生物染料，该染料用于在体外或体内检测样品中的pH。 本文还描述了用于制备用于所公开的组合物，方法和试剂盒的所述染料的方法。

公开(公告)号：US11841370B2

公开(公告)日：2023-12-12

申请号：US17492959

申请日：2021-10-04

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Levente Egry ,  Kyle Gee

IPC: G01N33/68 ,  G01N33/58 ,  C07F5/02 ,  C09B23/04 ,  C09B57/00 ,  G01N33/52

CPC classification number: G01N33/6803 ,  C07F5/022 ,  C09B23/04 ,  C09B57/00 ,  G01N33/52 ,  G01N33/582 ,  G01N33/68

Abstract: According to the present teachings, systems, compositions, kits and methods for protein melt analysis are provided that utilizing a dye that is a dipyrrometheneboron difluoride compound. In some embodiments, a method comprises preparing a sample by mixing at least one protein with a dye, and applying a controlled heating, while recording the fluorescence emission of the sample.

公开(公告)号：US11768156B2

公开(公告)日：2023-09-26

申请号：US17936947

申请日：2022-09-30

Applicant: Life Technologies Corporation

Inventor： Kyle Gee ,  Kathleen Kihn ,  Yi-Zhen Hu ,  Yexin Wu ,  Kathleen Free

IPC: G01N21/64 ,  C07D311/82 ,  C07D209/10 ,  C07F15/00 ,  C07F9/24

CPC classification number: G01N21/6486 ,  C07D209/10 ,  C07D311/82 ,  C07F9/2466 ,  C07F15/0093 ,  G01N21/6428

Abstract: Provided herein are compounds, compositions, kits, uses, and methods for assessing the viability of cells and/or quantifying the amount of live and dead cells in a mixture using differential stains. In some embodiments, the compounds are used in culture media or can function independently of fixation and/or permeabilization. In some embodiments, the compounds comprise a platinum atom. In some embodiments, the compounds comprise a nitrogen mustard moiety.

公开(公告)号：US11175204B1

公开(公告)日：2021-11-16

申请号：US15192107

申请日：2016-06-24

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jixiang Liu ,  Hee Chol Kang ,  Kyle Gee ,  Christopher Langsdorf ,  Jolene Bradford ,  Gayle Buller

IPC: C07D311/00 ,  G01N1/30 ,  C07D405/12 ,  C07D405/14 ,  G01N33/50

Abstract: Described herein are compounds, methods, and kits for long-term tracking of cell proliferation, differentiation, and/or function. The compounds of the present invention are novel cell-tracking reagents, efficiently excitable with a 405-nm violet laser, that provide bright fluorescence intensity, uniform cell staining, and good retention within cells as well as low toxicity toward cells.

公开(公告)号：US20190195801A1

公开(公告)日：2019-06-27

申请号：US16205702

申请日：2018-11-30

Applicant: Life Technologies Corporation

Inventor： Kyle Gee ,  Kathleen Kihn ,  Yi-Zhen Hu ,  Yexin Wu ,  Kathleen Free

IPC: G01N21/64 ,  C07F15/00 ,  C07D209/10 ,  C07D311/82

CPC classification number: G01N21/6486 ,  C07D209/10 ,  C07D311/82 ,  C07F15/0093 ,  G01N21/6428

Abstract: Provided herein are compounds, compositions, kits, uses, and methods for assessing the viability of cells and/or quantifying the amount of live and dead cells in a mixture using differential stains. In some embodiments, the compounds are used in culture media or can function independently of fixation and/or permeabilization. In some embodiments, the compounds comprise a platinum atom. In some embodiments, the compounds comprise a nitrogen mustard moiety.

公开(公告)号：US10246410B2

公开(公告)日：2019-04-02

申请号：US15529437

申请日：2015-12-03

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Wenjun Zhou ,  Kyle Gee ,  Yolanda Tennico ,  Peter Slade ,  Hee Chol Kang ,  Shaheer Khan ,  Brian Evans ,  James Stray

IPC: C07C309/47 ,  G01N33/58 ,  C07D311/84 ,  C07C309/51

Abstract: The present disclosure is directed dye compounds containing a hydrazinyl substituent and optionally, one or more negatively charged groups, such as sulfonate, phosphate, phosphonate, and/or carboxylate groups and dye compounds containing an aminooxy substitutent. The compounds are useful in the detection of analytes containing aldehyde and ketone groups, including, for example, glycans.

公开(公告)号：US20190079095A1

公开(公告)日：2019-03-14

申请号：US16107762

申请日：2018-08-21

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kyle Gee ,  Upinder Singh ,  Scott Grecian ,  Scott Clarke

IPC: G01N33/58 ,  G01N33/533 ,  C07D213/68 ,  C07D213/53 ,  C07D235/14 ,  C07D401/04 ,  C07D215/12 ,  C07D213/79 ,  C07D213/74 ,  C07K14/575 ,  C07D401/14 ,  C07D213/78 ,  C07D213/81

Abstract: Low-copper click chemistry, 1.3-dipolar cycloadditions, and Staudinger ligations for modifying biomolecules is provided. Compositions, methods, and kits relating to low-copper click chemistry, 1.3-dipolar cycloadditions, and Staudinger ligations are also provided.

公开(公告)号：US10087280B2

公开(公告)日：2018-10-02

申请号：US15491650

申请日：2015-08-14

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kyle Gee ,  Xin Wang ,  Hee Chol Kang ,  Aimei Chen ,  Yexin Wu ,  Yi-Zhen Hu ,  Robert Aggeler ,  Quentin Low

IPC: C08G61/12 ,  C07F7/08 ,  C08L65/00 ,  G01N33/533 ,  G01N33/58 ,  H01L51/00 ,  C09K11/06 ,  G01N33/545

Abstract: Water-soluble, conjugated polymers containing one or more dibenzosilole monomer residues, as well as compositions, kits, and methods of making and using such polymers are disclosed. Also disclosed are dibenzosilole derivatives substituted with one or more water-solubilizing groups, and methods of making and using such derivatives to prepare water-soluble dibenzosilole polymers.

公开(公告)号：US09645153B2

公开(公告)日：2017-05-09

申请号：US14829346

申请日：2015-08-18

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Evan Antoulinakis ,  Kyle Gee ,  Aleksey Rukavishnikov

IPC: G01N33/58 ,  C07D311/18 ,  C07D495/22 ,  C07D493/14 ,  C07D209/18 ,  C07F5/02 ,  C09B11/26 ,  C09B45/00 ,  C07D311/82 ,  G01N33/532 ,  C07D401/10 ,  C07D209/14

CPC classification number: G01N33/58 ,  C07D209/14 ,  C07D209/18 ,  C07D311/18 ,  C07D311/82 ,  C07D401/10 ,  C07D493/14 ,  C07D495/22 ,  C07F5/022 ,  C09B11/24 ,  C09B11/26 ,  C09B23/04 ,  C09B23/08 ,  C09B45/00 ,  C09B56/16 ,  G01N33/532 ,  G01N33/582

Abstract: The present disclosure is directed to a reactive ester agent capable of conjugating a reporter molecule to a carrier molecule or solid support. The reactive ester agent has the general formula: wherein the variables are described throughout the application.

公开(公告)号：US09599622B2

公开(公告)日：2017-03-21

申请号：US14402358

申请日：2013-03-14

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kyle Gee ,  Upinder Singh ,  Aleksey Rukavishnikov ,  Daniel Beacham ,  Shih-Jung Huang ,  Michael Janes ,  Wenjun Zhou

IPC: C09B11/24 ,  G01N33/84 ,  C07D311/90 ,  G01N33/58 ,  A61K49/00 ,  G01N33/50

CPC classification number: C09B11/24 ,  A61K49/0041 ,  C07D311/90 ,  G01N33/5005 ,  G01N33/582 ,  G01N33/84

Abstract: Disclosed herein are compounds, compositions, methods and kits for detecting pH in samples using pH-sensitive fluorescent dyes. The compounds disclosed herein are novel xanthene-derivative dyes comprising an aniline moiety with one or more electron donating groups, which dyes are for detecting pH in samples either in vitro or in vivo. Also described herein are processes for preparing said dyes for use in the disclosed compositions, methods and kits.