利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

92 条记录 (耗时 0.025 秒)

    Methods and compositions for detecting and identifying species of Candida
    13.
    发明授权
    Methods and compositions for detecting and identifying species of Candida 有权
    用于检测和鉴定假丝酵母菌种的方法和组合物

    公开(公告)号：US08501408B2

    公开(公告)日：2013-08-06

    申请号：US11321984

    申请日：2005-12-29

    申请人： Jason Trama ,  Martin E. Adelson ,  Eli Mordechai

    发明人： Jason Trama ,  Martin E. Adelson ,  Eli Mordechai

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6895 ,  C12Q1/689

    摘要： Methods and compositions useful in the detection and identification of species of Candida are disclosed. The compositions are combinations of oligonucleotides, where the forward primers of the primer pairs have identical sequences, while each reverse primer of the primer pairs has a unique sequence relative to all of the other reverse primers; or the reverse primers of the primer pairs have identical sequences, while each forward primer of the primer pairs has a unique sequence relative to all of the other forward primers. The oligonucleotides also include probes capable of detecting these amplicons, and sequencing primers for determining, in primer extension reactions, the nucleotide sequences contained within the amplicons. The detection of an amplicon indicates that the sample contains at least one isolate of Candida albicans, Candida glabrata, Candida parapsilosis, or Candida tropicalis, and the nucleotide sequence data is used to determine which of these four Candida species is present.

    摘要翻译： 公开了用于检测和鉴定假丝酵母菌种的方法和组合物。 组合物是寡核苷酸的组合，其中引物对的正向引物具有相同的序列，而引物对的每个反向引物相对于所有其它反向引物具有唯一的序列; 或引物对的反向引物具有相同的序列，而引物对的每个正向引物相对于所有其它正向引物具有唯一的序列。 寡核苷酸还包括能够检测这些扩增子的探针和测序引物，用于在引物延伸反应中测定扩增子中包含的核苷酸序列。 扩增子的检测表明该样品含有至少一种白色念珠菌，光滑假丝酵母（Candida glabrata），假丝酵母​​（Candida parapsilosis）或热带假丝酵母（Candida tropicalis））的分离物，并且使用核苷酸序列数据来确定这四种假丝酵母属中存在哪一种。

    Novel single nucleotide polymorphisms and community-associated methicillin-resistant staphylococcus aureus
    15.
    发明申请
    Novel single nucleotide polymorphisms and community-associated methicillin-resistant staphylococcus aureus 有权
    新型单核苷酸多态性和社区相关耐甲氧西林金黄色葡萄球菌

    公开(公告)号：US20120009572A1

    公开(公告)日：2012-01-12

    申请号：US13068331

    申请日：2011-05-09

    申请人： Scott E. Gygax ,  Sean Chadwick ,  Aditya Prasad ,  Martin E. Adelson ,  Eli Mordechai

    发明人： Scott E. Gygax ,  Sean Chadwick ,  Aditya Prasad ,  Martin E. Adelson ,  Eli Mordechai

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/689 ,  C12Q2600/156

    摘要： The present invention is based on the discovery of novel polymorphisms (SNPs) in the penicillin binding protein (pbp3) gene in Staphylococcus aureus. The presence of G88A and/or G2047A SNPs provides an accurate, reliable biomarker for the presence of Methicillin Resistant Staphylococcus aureus (MRSA), specifically the community-associated MRSA (CA-MRSA). The present invention provides reagents used for detecting the SNPs as well as methods of identifying and using these variants to screen subjects for presence of CA-MRSA. The methods involve isolating a biological sample from a mammal (preferably a human) and testing for the presence of a SNP in the pbp3 gene which is associated with CA-MRSA.

    摘要翻译： 本发明基于在金黄色葡萄球菌中青霉素结合蛋白（pbp3）基因中新型多态性（SNP）的发现。 G88A和/或G2047A SNP的存在为耐甲氧西林金黄色葡萄球菌（MRSA），特别是社区相关MRSA（CA-MRSA）的存在提供了准确，可靠的生物标志物。 本发明提供用于检测SNP的试剂以及鉴定和使用这些变体筛选受试者存在CA-MRSA的方法。 所述方法包括从哺乳动物（优选人）分离生物样品并测试与CA-MRSA相关的pbp3基因中SNP的存在。

    Compositions and methods for detecting Atopobium vaginae
    16.
    发明授权
    Compositions and methods for detecting Atopobium vaginae 有权
    组合物和检测阴道的方法

    公开(公告)号：US07745595B2

    公开(公告)日：2010-06-29

    申请号：US11502694

    申请日：2006-08-10

    申请人： Jason Trama ,  Martin E. Adelson ,  Eli Mordechai

    发明人： Jason Trama ,  Martin E. Adelson ,  Eli Mordechai

    IPC分类号： C07H21/04 ,  C12Q1/68

    CPC分类号： C12Q1/689

    摘要： Disclosed are oligonucleotides useful in methods for determining whether a sample contains Atopobium vaginae or has an increased likelihood of containing Atopobium vaginae, an organism which is seen in conjunction with bacterial vaginosis or is a causative agent of bacterial vaginosis. These oligonucleotides, which have nucleotide sequences derived from a segment of the genome of Atopobium vaginae, are useful as forward and reverse primers for a polymerase chain reaction using nucleic acids from a biological sample as a template, and as probes for detecting any resultant amplicon. Detection of an amplicon indicates the sample contains Atopobium vaginae or has an increased likelihood of containing Atopobium vaginae.

    摘要翻译： 公开了用于确定样品是否含有阴道弧菌的方法中的寡核苷酸或具有增加的可能性，其含有与细菌性阴道炎联合观察的生物体或阴道细菌性阴道炎的致病因子的阴道角膜的可能性增加。 这些寡核苷酸具有源自阴道角膜的基因组的片段的核苷酸序列，可用作使用来自生物样品的核酸作为模板的聚合酶链反应的正向和反向引物，以及用于检测任何所得扩增子的探针。 扩增子的检测表明样品含有阴道角膜，或具有增加阴道角化的可能性。

    Compositions and methods for detecting human metapneumovirus
    17.
    发明申请
    Compositions and methods for detecting human metapneumovirus 审中-公开
    用于检测人类偏肺病毒的组合物和方法

    公开(公告)号：US20080213749A1

    公开(公告)日：2008-09-04

    申请号：US11713408

    申请日：2007-03-02

    申请人： Melanie Feola ,  Larisa Gofman ,  Eli Mordechai ,  Martin E. Adelson

    发明人： Melanie Feola ,  Larisa Gofman ,  Eli Mordechai ,  Martin E. Adelson

    IPC分类号： C07H21/00 ,  C12Q1/68 ,  C12Q1/70

    CPC分类号： C12Q1/701

    摘要： Disclosed are oligonucleotides useful in methods for determining whether a sample contains a human metapneumovirus or has an increased likelihood of containing a human metapneumovirus, a virus which is a causative agent of respiratory tract disease in humans. These oligonucleotides, which have nucleotide sequences derived from a coding segment of the gene encoding the fusion protein of a human metapneumovirus, are useful as forward and reverse primers for a polymerase chain reaction using reverse transcripts of RNA from a biological sample as templates, and as probes for detecting any resultant amplicon. Detection of an amplicon indicates the sample contains a human metapneumovirus or has an increased likelihood of containing a human metapneumovirus.

    摘要翻译： 公开了用于确定样品是否含有人类偏肺病毒或具有增加的可能性的可能性的寡核苷酸，所述可能性包含人类偏肺病毒，人类偏肺病毒是人类呼吸道疾病的致病因子的病毒。 具有源自编码人类偏肺病毒融合蛋白的基因的编码区的核苷酸序列的这些寡核苷酸可用作使用来自生物样品的RNA的逆转录作为模板的聚合酶链反应的正向引物和反向引物，以及 用于检测任何所得扩增子的探针。 扩增子的检测表明样品含有人类偏肺病毒，或具有增加含有人间质肺病毒的可能性。

    Antibiotic resistance profile for Neisseria gonorrhoeae and use of same in diagnosis and treatment of gonorrhea
    19.
    发明授权
    Antibiotic resistance profile for Neisseria gonorrhoeae and use of same in diagnosis and treatment of gonorrhea 有权
    淋病奈瑟氏球菌的抗生素耐药性及其在淋病诊断和治疗中的应用

    公开(公告)号：US09297048B2

    公开(公告)日：2016-03-29

    申请号：US13134252

    申请日：2011-06-02

    申请人： Sergey Balashov ,  Eli Mordechai ,  Martin E. Adelson ,  Scott E. Gygax

    发明人： Sergey Balashov ,  Eli Mordechai ,  Martin E. Adelson ,  Scott E. Gygax

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/689 ,  C12Q2600/16

    摘要： The present invention relates to an antibiotic resistance profile for Neisseria gonorrhoeae by assessing the presence of mutations (e.g., SNP) in antibiotic resistant genes that confer bacterial resistance against antibiotics such as penicillin, tetracycline, fluoroquinolones, cephalosporin, macrolides and spectinomycin. There is provided a method and a kit for generating an antibiotic resistance profile for Neisseria gonorrhoeae by utilizing a multiplex PCR to amplify segments of antibiotic-resistant genes, allele-specific primer extension to detect gene mutation, and detection of such gene mutations with gel electrophoresis, capillary electrophoresis, or DNA microarray. The present method provides useful information to physicians relating the antibiotic susceptibility of Neisseria gonorrhoeae against different classes of antibiotics. Relying on this personalized diagnostic tool, physicians can better inform about antibiotic susceptibility and thereby open up medical intervention avenues for treating Neisseria gonorrhoeae with antibiotics. There is provided a therapeutic application of the antibiotic resistance profile that has advantages of: (i) providing a more effective regime for gonorrhea treatment; and (ii) halting the evolutionary pressures towards antibiotic resistance in the Neisseria gonorrhoeae therapy.

    摘要翻译： 本发明涉及通过评估抗生素抗性基因中的突变（例如SNP）的存在，其对抗生素如青霉素，四环素，氟喹诺酮，头孢菌素，大环内酯和壮观霉素提供细菌耐药性，涉及淋病奈瑟氏球菌抗生素抗性谱。 提供了一种通过利用多重PCR扩增抗生素抗性基因片段，等位基因特异性引物延伸以检测基因突变以及用凝胶电泳检测这种基因突变来产生淋病奈瑟氏球菌抗生素抗性谱的方法和试剂盒 ，毛细管电泳或DNA微阵列。 本方法为医生提供了与淋病奈瑟氏球菌对不同类型抗生素的抗生素敏感性有关的有用信息。 依靠这种个性化诊断工具，医生可以更好地了解抗生素敏感性，从而开辟用抗生素治疗淋病奈瑟氏球菌的医疗干预途径。 提供了抗生素抗性谱的治疗应用，其优点在于：（i）为淋病治疗提供更有效的方案; 和（ii）阻止淋病奈瑟氏球菌治疗中抗生素耐药性的进化压力。

    Oligonucleotides useful in methods for detecting and characterizing Aspergillus fumigatus
    20.
    发明授权
    Oligonucleotides useful in methods for detecting and characterizing Aspergillus fumigatus 有权
    用于检测和表征烟曲霉的方法中的寡核苷酸

    公开(公告)号：US09145593B2

    公开(公告)日：2015-09-29

    申请号：US13461122

    申请日：2012-09-17

    申请人： Jason Trama ,  Martin E Adelson ,  Eli Mordechai

    发明人： Jason Trama ,  Martin E Adelson ,  Eli Mordechai

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6895 ,  C12Q1/689 ,  C12Q2561/113 ,  C12Q2565/301 ,  C12Q2600/156

    摘要： Methods for using oligonucleotides in the detection of Aspergillus fumigatus are disclosed. The oligonucleotides of the invention have nucleotide sequences derived from the gene encoding the cytochrome P450 14 alpha-sterol demethylase (the cyp51A protein) of Aspergillus fumigatus. The oligonucleotides include primers capable of producing amplicons specific to cyp51A in polymerase chain reactions using nucleic acids isolated from Aspergillus fumigatus as templates. The oligonucleotides also include probes capable of detecting these cyp51A-specific amplicons. The oligonucleotides of the invention also include primers for nucleotide sequencing reactions to determine whether an isolate of Aspergillus fumigatus is more tolerant than wild-type Aspergillus fumigatus to a triazole.

    摘要翻译： 公开了在检测烟曲霉中使用寡核苷酸的方法。 本发明的寡核苷酸具有衍生自编码烟曲霉的细胞色素P45014α-固醇脱甲基酶（cyp51A蛋白）的基因的核苷酸序列。 寡核苷酸包括能够使用从烟曲霉分离的核酸作为模板在聚合酶链反应中产生cyp51A特异性扩增子的引物。 寡核苷酸还包括能够检测这些cyp51A特异性扩增子的探针。 本发明的寡核苷酸还包括用于核苷酸测序反应的引物，以确定烟曲霉分离物是否比野生型烟曲霉对三唑的耐受性更高。