RNA SEQUENCING AND ANALYSIS USING SOLID SUPPORT
    12.
    发明申请
    RNA SEQUENCING AND ANALYSIS USING SOLID SUPPORT 审中-公开
    使用固体支持的RNA测序和分析

    公开(公告)号:US20100035249A1

    公开(公告)日:2010-02-11

    申请号:US12186009

    申请日:2008-08-05

    IPC分类号: C12Q1/68

    摘要: The present invention provides methods for the sequencing of all RNA species within an RNA sample, such as the RNA content obtained from a cell, a tissue, a living organism, or from an artificial source. RNA molecules within the samples are labeled in a RNA-specific manner prior to immobilization on a solid support. One label is used to mark the location of the RNA molecule on the solid support, whereas the second label is used to mark selectively the S′ end of full-length mRNA molecules. RNA molecules are sequenced while being bound to the solid support in one or more sequencing reactions, and sequences of individual RNA molecules can be forwarded to computational analysis for assembling sequence information from individual sequencing reads obtained from the same location on the solid support. Not only unsupervised expression profiling on a genome-wide scale, but also the direct analysis of RNA-RNA interactions become possible as revealed by the analysis of the sequencing information obtained along with genomic information.

    摘要翻译: 本发明提供了用于对RNA样品中所有RNA物种进行测序的方法,例如从细胞,组织,活生物体或人造来源获得的RNA含量。 在固定在固体支持物上之前,将样品中的RNA分子以RNA特异性方式标记。 一个标签用于标记RNA分子在固体支持物上的位置,而第二个标记用于选择性标记全长mRNA分子的S'末端。 RNA分子在一个或多个测序反应中与固体支持物结合时进行测序,并且可将单个RNA分子的序列转发到计算分析,以从固体支持物上的相同位置获得的单独测序读数组装序列信息。 不仅在基因组范围内无监督的表达谱,而且通过分析与基因组信息一起获得的测序信息显示,RNA-RNA相互作用的直接分析也是可能的。

    Shift control device of transmission
    13.
    发明授权
    Shift control device of transmission 失效
    变速器变速控制装置

    公开(公告)号:US5134903A

    公开(公告)日:1992-08-04

    申请号:US720742

    申请日:1991-06-25

    申请人: Masayoshi Itoh

    发明人: Masayoshi Itoh

    摘要: A shift control device includes a forward and reverse shifting mechanism for selectively drivingly connecting and disconnecting forward and reverse gear trains to and from a vehicle drive wheel. In shifting the transmission from reverse through neutral and into drive, the forward and reverse shift mechanism remains operatively engaged with the reverse gear train until the transmission is shifted from neutral into drive, at which time the forward and reverse shift mechanism moves into operative engagement with the forward gear train. In shifting from drive through neutral into reverse, the forward and reverse shift mechanism remains operatively engaged with the forward gear train until the transmission is shifted from neutral into reverse, at which time the forward and reverse shift mechanism is moved into operative engagement with the reverse gear train. Accordingly to another embodiment, if the forward and reverse shift mechanism becomes improperly engaged with one of the gear trains, this improper engagement is detected, and the forward and reverse shift mechanism is automatically adjusted by a slight amount to permit proper engagement with the gear train.

    摘要翻译: 变速控制装置包括前进和后退换档机构,用于选择性地驱动地连接和断开前进和后退齿轮系进出车辆驱动轮。 在将变速器从反向变速器通过空档转换为驱动时,前进和后退换档机构保持与倒档齿轮系可操作地接合,直到变速器从空档移动到驱动器中,此时前进和后退换档机构移动到与 前进齿轮系。 在从变速器通过中性线转向反向时,前进和后退换档机构保持与前进齿轮系统可操作地接合,直到变速器从空档转移到反向,此时前进和后退换档机构被移动到与倒档 齿轮系。 因此,在另一实施方式中,如果前后换挡机构与其中一个齿轮系不正确地接合,则检测到这种不正确的接合,并且前后换档机构自动调节少量以允许与齿轮系正确接合 。