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    System for controlling the output power of motor vehicle
    2.
    发明授权
    System for controlling the output power of motor vehicle 失效
    控制电机输出功率的系统

    公开(公告)号：US5245542A

    公开(公告)日：1993-09-14

    申请号：US661293

    申请日：1991-02-27

    申请人： Masayoshi Itoh ,  Susumu Nishikawa ,  Yasunobu Miyata

    发明人： Masayoshi Itoh ,  Susumu Nishikawa ,  Yasunobu Miyata

    IPC分类号： F02D41/04 ,  B60K28/16 ,  F02D9/02 ,  F02D11/10 ,  F02D29/02 ,  F02D45/00 ,  F02P5/15

    CPC分类号： B60K28/16 ,  B60W2520/105 ,  B60W2520/125 ,  B60W2530/16 ,  B60W2710/0605 ,  B60W2710/0666 ,  B60W2720/106

    摘要： A motor vehicle output power control system effects a slip control process for preventing the drive wheels of a motor vehicle from slipping against unstable maneuverability and a trace control process for preventing the motor vehicle from failing to make a turn due to an excessive lateral acceleration. The motor vehicle output power control system has a torque control unit for reducing the drive torque produced by the engine irrespective of controlling action of the driver. A target speed for the drive wheels is established on the basis of the motor vehicle speed, and a reference drive torque for the engine is set depending on the target speed for the drive wheels. Based on a slip of the drive wheels, a target drive torque for the engine is established. The torque control unit is controlled by an electronic controller so that the drive torque produced by the engine is equalized to the target drive torque. The reference drive torque is corrected on the basis of the slip of the drive wheels, thus calculating the target drive torque. The drive torque of the engine can be quickly lowered in order not to unduly exceed maximum frictional forces between the road surface and the drive wheels. The motor vehicle is controlled by the motor vehicle output power control system to run safely with improved control responses because of a reduced number of corrective factors for control.

    摘要翻译： 机动车辆输出功率控制系统执行滑动控制处理，以防止机动车辆的驱动轮滑动不稳定的机动性，以及用于防止机动车辆由于过度的横向加速度而导致转弯的跟踪控制过程。 机动车辆输出功率控制系统具有用于降低发动机产生的驱动转矩的转矩控制单元，而与驾驶员的控制动作无关。 基于机动车辆速度建立驱动轮的目标速度，并且根据驱动轮的目标速度来设定发动机的基准驱动转矩。 基于驱动轮的滑动，建立了用于发动机的目标驱动扭矩。 扭矩控制单元由电子控制器控制，使得由发动机产生的驱动扭矩与目标驱动转矩相等。 基于驱动轮的滑移校正基准驱动转矩，由此计算目标驱动转矩。 可以快速降低发动机的驱动扭矩，以免过度地超过路面和驱动轮之间的最大摩擦力。 机动车辆由机动车辆输出功率控制系统控制，由于减少了用于控制的校正因子的数量，安全地运行并改进了控制响应。

    Novel DNA Polymerase
    3.
    发明申请
    Novel DNA Polymerase 审中-公开
    新型DNA聚合酶

    公开(公告)号：US20100047862A1

    公开(公告)日：2010-02-25

    申请号：US12083695

    申请日：2006-10-20

    申请人： Yoshihide Hayashizaki ,  Masayoshi Itoh ,  Yoshimi Benno ,  Alexander Lezhava

    发明人： Yoshihide Hayashizaki ,  Masayoshi Itoh ,  Yoshimi Benno ,  Alexander Lezhava

    IPC分类号： C12P21/00 ,  C12N9/12 ,  C07H21/00 ,  C12N15/63 ,  C12N1/00 ,  C12P19/34

    CPC分类号： C12N9/1276 ,  C12N9/1252

    摘要： This invention provides a novel DNA polymerase obtained from Bacillus smithii JCM9076, which has novel features in terms of, for example, optimal reaction conditions (e.g., optimal temperature) and enzyme activity. More particularly, a novel DNA polymerase is a pol I type DNA polymerase, which is any of proteins (a) to (f) below and has DNA polymerase activity: (a) a protein comprising the amino acid sequence as shown in SEQ ID NO: 7; (b) a protein consisting of an amino acid sequence derived from the amino acid sequence as shown in SEQ ID NO: 7 by deletion, substitution, or addition of one or several amino acid residues; (c) a protein consisting of the amino acid sequence as shown in SEQ ID NO: 9; and (d) a protein consisting of an amino acid sequence derived from the amino acid sequence as shown in SEQ ID NO: 9 by deletion, substitution, or addition of one or several amino acid residues.

    摘要翻译： 本发明提供了一种从芽孢杆菌JCM9076获得的新型DNA聚合酶，其在例如最佳反应条件（例如最佳温度）和酶活性方面具有新特征。 更具体地，新型DNA聚合酶是pol I型DNA聚合酶，其是以下蛋白质（a）至（f）中的任一种，并且具有DNA聚合酶活性：（a）包含SEQ ID NO所示的氨基酸序列的蛋白质 ：7; （b）通过缺失，取代或添加一个或几个氨基酸残基的由SEQ ID NO：7所示的氨基酸序列衍生的氨基酸序列组成的蛋白质; （c）由SEQ ID NO：9所示的氨基酸序列组成的蛋白质; 和（d）通过缺失，取代或添加一个或几个氨基酸残基，由SEQ ID NO：9所示的氨基酸序列衍生的氨基酸序列组成的蛋白质。

    ISOTHERMAL AMPLIFICATION METHOD AND DNA POLYMERASE USED IN THE SAME
    4.
    发明申请
    ISOTHERMAL AMPLIFICATION METHOD AND DNA POLYMERASE USED IN THE SAME 审中-公开
    同位素放大方法和使用其中的DNA聚合酶

    公开(公告)号：US20100221787A1

    公开(公告)日：2010-09-02

    申请号：US12739496

    申请日：2008-10-24

    申请人： Yoshihide Hayashizaki ,  Masayoshi Itoh ,  Alexander Lezhava ,  Yoshimi Benno ,  Yasumasa Mitani ,  Hajime Kanamori

    发明人： Yoshihide Hayashizaki ,  Masayoshi Itoh ,  Alexander Lezhava ,  Yoshimi Benno ,  Yasumasa Mitani ,  Hajime Kanamori

    IPC分类号： C12P19/34 ,  C12N9/12

    CPC分类号： C12Q1/6846 ,  C12N9/1252 ,  C12P19/34 ,  C12Q2525/155 ,  C12Q2521/107 ,  C12Q2527/101 ,  C12Q2531/107

    摘要： A DNA polymerase suitable for specific isothermal amplification methods and an isothermal amplification method using the DNA polymerase are provided. In the presence of a DNA polymerase including a protein described in the following item (a) or (b), an amplification reaction of a target nucleic acid sequence in a nucleic acid sample is carried out isothermally using a first primer shown in the following (X). By using the DNA polymerase, it becomes possible to carry out the amplification reaction using the primer within a shorter time than ever before. (a) a protein having an amino acid sequence represented by SEQ ID NO. 23 (b) a protein having an amino acid sequence represented by SEQ ID NO. 25 (X) a primer that contains, in a 3′ end portion, a sequence (Ac′) that hybridizes to a sequence (A) of a 3′ end portion of the target nucleic acid sequence and also contains, on a 5′ side of the sequence (Ac′), a sequence (B′) that hybridizes to a complementary sequence (Bc) to a sequence (B) present on a 5′ side with respect to the sequence (A) in the target nucleic acid sequence

    摘要翻译： 提供了适用于特定等温扩增方法的DNA聚合酶和使用DNA聚合酶的等温扩增方法。 在包含下述（a）或（b）中所述的蛋白质的DNA聚合酶的存在下，使用下述第一引物等温进行靶核酸序列的扩增反应 X）。 通过使用DNA聚合酶，可以在比以往更短的时间内使用引物进行扩增反应。 （a）具有SEQ ID NO：1所示的氨基酸序列的蛋白质。 23（b）具有SEQ ID NO：1所示的氨基酸序列的蛋白质。 25（X）引物，其在3'末端含有与目标核酸序列的3'端部分的序列（A）杂交的序列（Ac'），并且在5' 序列（Ac'）侧，与靶序列（A）相对于存在于5'侧的序列（B）的互补序列（Bc）杂交的序列（B'）

    NOVEL MUTS PROTEIN AND METHOD FOR DETERMING MUTATION USING THE SAME
    7.
    发明申请
    NOVEL MUTS PROTEIN AND METHOD FOR DETERMING MUTATION USING THE SAME 审中-公开
    新颖的蛋白质和使用其确定突变的方法

    公开(公告)号：US20110236900A1

    公开(公告)日：2011-09-29

    申请号：US13130993

    申请日：2009-11-27

    申请人： Yoshihide Hayashizaki ,  Masayoshi Itoh ,  Hajime Kanamori ,  Kengo Usui ,  Kazuhito Nomura

    发明人： Yoshihide Hayashizaki ,  Masayoshi Itoh ,  Hajime Kanamori ,  Kengo Usui ,  Kazuhito Nomura

    IPC分类号： C12Q1/68 ,  C07K14/195 ,  C07H21/04 ,  C12N5/10 ,  C12P21/06 ,  C12N1/00

    CPC分类号： C07K14/195 ,  G01N33/5308 ,  G01N2333/32

    摘要： A method for determining the presence or absence of a mutation on the basis of the presence or absence of amplification with high reliability is provided. A target sequence including a target site contained in a sample nucleic acid is amplified using a primer that can hybridize to a region including the target site contained in the sample nucleic acid in the presence of a novel MutS having an amino acid sequence of SEQ ID NO: 2, and then the presence or absence of a mutation at the target site is determined on the basis of the presence or absence of amplification. The novel MutS binds more specifically to a mismatched base pair than to a fully-matched base pair, whereby an extension reaction caused by a mismatch-binding primer is suppressed. Thus, according to the present invention, the presence or absence of a mutation can be determined with high reliability.

    摘要翻译： 提供了一种基于高可靠性的扩增的存在或不存在来确定突变的存在或不存在的方法。 包含样品核酸中包含的靶位点的靶序列使用可以在含有氨基酸序列SEQ ID NO的新型MutS的存在下与包含在样品核酸中的靶位点的区域杂交的引物进行扩增 ：2，然后基于扩增的存在或不存在来确定靶位点处的突变的存在或不存在。 新的MutS更具体地结合失配的碱基对而不是完全匹配的碱基对，由此抑制由错配结合引物引起的延伸反应。 因此，根据本发明，可以高可靠性确定突变的存在或不存在。