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112 条记录 (耗时 0.028 秒)

    METHOD FOR ISOLATING AN ALKANOL FROM AN AQUEOUS BIOTRANSFORMATION MIXTURE
    11.
    发明申请
    METHOD FOR ISOLATING AN ALKANOL FROM AN AQUEOUS BIOTRANSFORMATION MIXTURE 审中-公开
    从水溶性生物混合物中分离出烷基醇的方法

    公开(公告)号：US20120285816A1

    公开(公告)日：2012-11-15

    申请号：US13511594

    申请日：2010-11-24

    申请人： Jürgen Däuwel ,  Michael Breuer ,  Bernhard Hauer

    发明人： Jürgen Däuwel ,  Michael Breuer ,  Bernhard Hauer

    IPC分类号： B01D3/36

    CPC分类号： C07C29/82 ,  C07B2200/07 ,  C07C29/80 ,  C07C29/86 ,  C07C31/12 ,  C07C31/125

    摘要： The invention relates to the isolation of an alkanol from an aqueous biotransformation mixture, in that a) a first alkanol phase is obtained by means of distilling out an alkanol-water azeotrope from the aqueous biotransformation mixture and, if the azeotrope is a heteroazeotrope, phase separating the azeotrope and separating out an aqueous phase, b) a second alkanol phase is obtained by (i) liquid/liquid extracting the first alkanol phase using a solvent as an extracting agent, or (ii) azeotropic drying the first alkanol phase in the presence of the solvent as a carrier agent, and c) the second alkanol phase is fractionally distilled, producing a pure alkanol fraction. The biotransformation mixture is obtained, for example, by means of reducing an alkanol in the presence of an alcohol dehydrogenase. The method is adapted to the severe dilution of the products of value in the biotransformation mixture and works without long phase separation times when extracting by means of organic solvents.

    摘要翻译： 本发明涉及从水生物转化混合物中分离链烷醇，其中a）通过从水生物转化混合物中蒸馏出链烷醇 - 水共沸物获得第一链烷醇相，如果共沸物是杂合物，则相 分离共沸物并分离水相，b）通过以下步骤获得第二链烷醇相：（i）使用溶剂作为萃取剂液/液萃取第一链烷醇相，或（ii）共沸干燥第一链烷醇相 作为载体剂的溶剂的存在，和c）分馏第二链烷醇相，产生纯的链烷醇级分。 生物转化混合物例如通过在醇脱氢酶的存在下还原链烷醇获得。 该方法适用于在生物转化混合物中严重稀释有价值的产物，并在通过有机溶剂萃取时无长时间分离时间。

    Enzymatic reduction for producing optically active alcohols
    16.
    发明授权
    Enzymatic reduction for producing optically active alcohols 有权
    用于制备光学活性醇的酶还原

    公开(公告)号：US07888080B2

    公开(公告)日：2011-02-15

    申请号：US12158390

    申请日：2006-12-13

    申请人： Rainer Stürmer ,  Maria Keβeler ,  Bernhard Hauer ,  Thomas Friedrich ,  Michael Breuer ,  Hartwig Schröder

    发明人： Rainer Stürmer ,  Maria Keβeler ,  Bernhard Hauer ,  Thomas Friedrich ,  Michael Breuer ,  Hartwig Schröder

    IPC分类号： C12P17/00 ,  C12N9/14

    CPC分类号： C07D333/16 ,  C07D333/20 ,  C12P7/02 ,  C12P11/00 ,  C12P41/002

    摘要： A process for preparing optically active alkanols of the formula I in which n is an integer from 0 to 5; Cyc is an optionally substituted, mono- or polynuclear, saturated or unsaturated, carbocyclic or heterocyclic ring, and R1 is halogen, SH, OH, NO2, NR2R3 or NR2R3R4+X−, with R2, R3 and R4 independently of one another being hydrogen or a lower alkyl or lower alkoxy radical and X− being a counterion, which process comprises incubating in a medium comprising alkanone of the formula II  in which n, Cyc and R1 are as defined above, an enzyme having a polypeptide sequence (i) SEQ ID NO: 1 or (ii) in which, compared to SEQ ID NO:1, up to 25% of the amino acid radicals have been altered by deletion, insertion, substitution or a combination thereof and which retains at least 50% of the enzymic activity of SEQ ID NO:1, with the compound of the formula II being enzymically reduced to give the compound of the formula I, and isolating the product formed.

    摘要翻译： 一种制备式I的光学活性链烷醇的方法，其中n为0至5的整数; Cyc是任选取代的单或多核饱和或不饱和的碳环或杂环，R1是卤素，SH，OH，NO2，NR2R3或NR2R3R4 + X-，R2，R3和R4彼此独立地是氢 或低级烷基或低级烷氧基，X是抗衡离子，该方法包括在包含式II的烷酮的培养基中孵育，其中n，Cyc和R 1如上定义，具有多肽序列（i）SEQ ID NO： SEQ ID NO：1或（ii）其中与SEQ ID NO：1相比，通过缺失，插入，取代或其组合改变多达25％的氨基酸基团，并且其保留至少50％的 酶活性为SEQ ID NO：1，其中式II化合物被酶还原，得到式I化合物，并分离形成的产物。

    Method For Producing Optically Active Alcohols From Alkanones Using a Dehydrogenase of Azoarcus
    18.
    发明申请
    Method For Producing Optically Active Alcohols From Alkanones Using a Dehydrogenase of Azoarcus 有权
    使用Azoarcus脱氢酶从烷醇生产光活性醇的方法

    公开(公告)号：US20080206824A1

    公开(公告)日：2008-08-28

    申请号：US11579292

    申请日：2005-05-04

    申请人： Rainer Sturmer ,  Maria Kesseler ,  Bernhard Hauer ,  Thomas Friedrich ,  Michael Breuer ,  Hartwig Schroder

    发明人： Rainer Sturmer ,  Maria Kesseler ,  Bernhard Hauer ,  Thomas Friedrich ,  Michael Breuer ,  Hartwig Schroder

    IPC分类号： C12P17/00 ,  C12P7/22

    CPC分类号： C07D333/22 ,  C07D333/16 ,  C12P17/00

    摘要： The invention relates to a method for producing the optically active alkanols of formula (I), wherein n is an integer of from 0 to 5; Cyc represents an optionally substituted, mononuclear or polynuclear, saturated or unsaturated, carbocylic or heterocyclic ring, and R1 represents halogen, SH, OH, NO2, NR2R3 or NR2R3R4+X−, wherein R2, R3 and R4 independently represent H or a lower alkyl or lower alkoxy group and X− represents a counterion. According to the invention, an enzyme (E) selected from the groups of dehydrogenases, aldehyde reductases and carbonyl reductases is incubated in a medium containing the alkanone of formula (II), wherein n, Cyc and R1 are defined as above, in the presence of reduction equivalents. The compound of formula (II) is enzymatically reduced to the compound of formula (I) and the reduction equivalents consumed during reaction are regenerated by reacting a sacrificial alcohol to the corresponding sacrificial ketone using enzyme (E) and at least partially removing the sacrificial ketone from the reaction medium, and then isolating the product (I) so produced.

    摘要翻译： 本发明涉及一种制备式（I）的光学活性链烷醇的方法，其中n为0-5的整数; Cyc表示任选取代的单核或多核，饱和或不饱和的碳环或杂环，R 1表示卤素，SH，OH，NO 2，NR 2/3/3或3/3/3/3/4/4 / 其中R 2，R 3和R 4独立地表示H或低级烷基或低级烷氧基，X - 表示抗衡离子。 根据本发明，将选自脱氢酶，醛还原酶和羰基还原酶的酶（E）在含有式（II）的烷酮的培养基中温育，其中n，Cyc和R 1 如上所述，在还原当量的存在下。 将式（II）化合物酶还原成式（I）化合物，并且通过使用酶（E）使牺牲醇与相应的牺牲酮反应，并且至少部分除去牺牲酮来再生反应期间消耗的还原当量 从反应介质中分离出如此制备的产物（I）。

    Preparation of beta-amino acids
    20.
    发明授权
    Preparation of beta-amino acids 有权
    β-氨基酸的制备

    公开(公告)号：US09096841B2

    公开(公告)日：2015-08-04

    申请号：US13496086

    申请日：2010-09-15

    申请人： Nina Schneider ,  Bernhard Hauer ,  Klaus Ditrich ,  Maeve O'Neil ,  Nick Turner

    发明人： Nina Schneider ,  Bernhard Hauer ,  Klaus Ditrich ,  Maeve O'Neil ,  Nick Turner

    IPC分类号： C12P13/04 ,  C12N9/86

    CPC分类号： C12N9/86 ,  C12P13/04

    摘要： The present invention relates to a process for the biocatalytic, enantioselective production of a β-amino acid precursor from an optionally substituted dihydrouracil using a hydantoinase and/or a dihydropyrimidinase, a process for producing a β-amino acid from said precursor, a hydantoinase and its use in said process for the biocatalytic production of a β-amino acid precursor or a β-amino acid, and a method for obtaining said hydantoinase.

    摘要翻译： 本发明涉及使用乙内酰脲酶和/或二氢嘧啶苷从任选取代的二氢尿嘧啶生物催化，对映选择性地生产“氨基酸”前体的方法，一种从所述前体制备一种氨基酸的方法， 乙内酰脲及其用于生物催化生产“氨基酸”前体或“氨基酸”的所述方法中的应用，以及用于获得所述乙内酰脲酶的方法。