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    Modified nitrilases and their use in methods for the production of carboxylic acids
    3.
    发明申请
    Modified nitrilases and their use in methods for the production of carboxylic acids 有权
    改性腈水解酶及其在羧酸生产方法中的应用

    公开(公告)号：US20060259999A1

    公开(公告)日：2006-11-16

    申请号：US10546611

    申请日：2004-02-24

    申请人： Thomas Zelinski ,  Maria Kesseler ,  Bernhard Hauer ,  Thomas Friedrich

    发明人： Thomas Zelinski ,  Maria Kesseler ,  Bernhard Hauer ,  Thomas Friedrich

    IPC分类号： C12P7/40 ,  C12N9/80 ,  C12N15/87

    CPC分类号： C12N9/80 ,  C12N9/78 ,  C12N15/8274 ,  C12P7/40 ,  C12P7/42

    摘要： The present Invention relates to new nitrilases with modified Substrate acceptance, the method to obtain them and the use of the aforesaid nitrilases. Said nitrilases are encoded by a polypeptide sequence, which at a Position corresponding to Position 296 in wild-type Alcaligenes faecalis comprises an amino acid which is not tyrosine. The Invention further relates to nucleic acid sequences and amino acid sequences encoding Said nitrilases, expression constructs comprising Said nucleic acid sequences, vectors comprising the nucleic acid sequences or the exPression constructs, to organisms, preferably microorganisms, comprising the nucleic acid sequences, expression constructs or vectors. The Invention additionally relates to a method for preparing carboxylic acids, preferably substituted chiral carboxylic acids from racemic nitriles.

    摘要翻译： 本发明涉及具有改进的基板接受性的新腈水解酶，获得它们的方法和使用上述腈水解酶。 所述腈水解酶由多肽序列编码，其在与野生型产碱杆菌粪便中的位置296相对应的位置包含不是酪氨酸的氨基酸。 本发明进一步涉及编码所述腈水解酶的核酸序列和氨基酸序列，包含所述核酸序列，包含核酸序列或exPression构建体的载体的表达构建体，包括核酸序列，表达构建体或生物体 向量 本发明还涉及从外消旋腈制备羧酸，优选取代的手性羧酸的方法。

    Enzymatic Reduction for Producing Optically Active Alcohols
    4.
    发明申请
    Enzymatic Reduction for Producing Optically Active Alcohols 有权
    用于生产光活性醇的酶减量

    公开(公告)号：US20080318288A1

    公开(公告)日：2008-12-25

    申请号：US12158390

    申请日：2006-12-13

    申请人： Rainer Stürmer ,  Maria Kesseler ,  Bernhard Hauer ,  Thomas Friedrich ,  Michael Breuer ,  Hartwig Schröder

    发明人： Rainer Stürmer ,  Maria Kesseler ,  Bernhard Hauer ,  Thomas Friedrich ,  Michael Breuer ,  Hartwig Schröder

    IPC分类号： C12P17/00 ,  C12N9/14

    CPC分类号： C07D333/16 ,  C07D333/20 ,  C12P7/02 ,  C12P11/00 ,  C12P41/002

    摘要： A process for preparing optically active alkanols of the formula I in which n is an integer from 0 to 5; Cyc is an optionally substituted, mono- or polynuclear, saturated or unsaturated, carbocyclic or heterocyclic ring, and R1 is halogen, SH, OH, NO2, NR2R3 or NR2R3R4+X−, with R2, R3 and R4 independently of one another being hydrogen or a lower alkyl or lower alkoxy radical and X− being a counterion, which process comprises incubating in a medium comprising alkanone of the formula II in which n, Cyc and R1 are as defined above, an enzyme having a polypeptide sequence (i) SEQ ID NO: 1 or (ii) in which, compared to SEQ ID NO:1, up to 25% of the amino acid radicals have been altered by deletion, insertion, substitution or a combination thereof and which retains at least 50% of the enzymic activity of SEQ ID NO:1, with the compound of the formula II being enzymically reduced to give the compound of the formula I, and isolating the product formed.

    摘要翻译： 一种制备式I的光学活性链烷醇的方法，其中n为0至5的整数; Cyc是任选取代的单或多核饱和或不饱和的碳环或杂环，R1是卤素，SH，OH，NO2，NR2R3或NR2R3R4 + X-，R2，R3和R4彼此独立地是氢 或低级烷基或低级烷氧基，X是抗衡离子，该方法包括在包含式II的烷酮的培养基中孵育，其中n，Cyc和R 1如上定义，具有多肽序列（i）SEQ ID NO： SEQ ID NO：1或（ii）其中与SEQ ID NO：1相比，通过缺失，插入，取代或其组合改变多达25％的氨基酸基团，并且其保留至少50％的 酶活性为SEQ ID NO：1，其中式II化合物被酶还原，得到式I化合物，并分离形成的产物。

    Method for the Enzymatic Production of 5-Norbornen-2-Carboxylic Acid
    8.
    发明申请
    Method for the Enzymatic Production of 5-Norbornen-2-Carboxylic Acid 审中-公开
    5-降冰片烯-2-羧酸的酶法生产方法

    公开(公告)号：US20080265206A1

    公开(公告)日：2008-10-30

    申请号：US12158378

    申请日：2006-12-11

    申请人： Maria Kesseler ,  Bernhard Haner

    发明人： Maria Kesseler ,  Bernhard Haner

    IPC分类号： C12P7/40 ,  C12N9/78 ,  C07K14/00 ,  C07H21/00 ,  C12N15/03 ,  C12N1/20 ,  C09K3/00

    CPC分类号： C12P7/40 ,  C07C2601/10 ,  C12N9/78 ,  C12P41/006

    摘要： The present invention relates to a process for the preparation of 5-norbornene-2-carboxylic acid from 5-norbornene-2-endo-carbonitrile and/or 5-norbornene-2-exo-carbonitrile. The invention relates in particular to a process which enables 5-norbornene-2-carboxylic acid to be prepared at a high substrate concentration. The invention furthermore relates to a polypeptide suitable for enzymatic conversion of 5-norbornene-2-carbonitrile to give 5-norbornene-2-carboxylic acid, in particular also with a high substrate concentration, and to a nucleic acid encoding said polypeptide, to a composition comprising 5-norbornene-2-carbonitrile to 5-norbornene-2-endo-carboxylic acid and 5-norbornene-2-exo-carboxylic acid, and to the use of said polypeptide.

    摘要翻译： 本发明涉及从5-降冰片烯-2-内酯和/或5-降冰片烯-2-外甲腈制备5-降冰片烯-2-羧酸的方法。 本发明具体涉及一种能够以高底物浓度制备5-降冰片烯-2-羧酸的方法。 本发明还涉及适用于5-降冰片烯-2-腈的酶促转化以产生5-降冰片烯-2-羧酸，特别是具有高底物浓度的多肽和编码所述多肽的核酸的多肽， 组合物包含5-降冰片烯-2-腈至5-降冰片烯-2-内羧酸和5-降冰片烯-2-外羧酸，以及所述多肽的用途。

    Method For Producing Optically Active Alcohols From Alkanones Using a Dehydrogenase of Azoarcus
    9.
    发明申请
    Method For Producing Optically Active Alcohols From Alkanones Using a Dehydrogenase of Azoarcus 有权
    使用Azoarcus脱氢酶从烷醇生产光活性醇的方法

    公开(公告)号：US20080206824A1

    公开(公告)日：2008-08-28

    申请号：US11579292

    申请日：2005-05-04

    申请人： Rainer Sturmer ,  Maria Kesseler ,  Bernhard Hauer ,  Thomas Friedrich ,  Michael Breuer ,  Hartwig Schroder

    发明人： Rainer Sturmer ,  Maria Kesseler ,  Bernhard Hauer ,  Thomas Friedrich ,  Michael Breuer ,  Hartwig Schroder

    IPC分类号： C12P17/00 ,  C12P7/22

    CPC分类号： C07D333/22 ,  C07D333/16 ,  C12P17/00

    摘要： The invention relates to a method for producing the optically active alkanols of formula (I), wherein n is an integer of from 0 to 5; Cyc represents an optionally substituted, mononuclear or polynuclear, saturated or unsaturated, carbocylic or heterocyclic ring, and R1 represents halogen, SH, OH, NO2, NR2R3 or NR2R3R4+X−, wherein R2, R3 and R4 independently represent H or a lower alkyl or lower alkoxy group and X− represents a counterion. According to the invention, an enzyme (E) selected from the groups of dehydrogenases, aldehyde reductases and carbonyl reductases is incubated in a medium containing the alkanone of formula (II), wherein n, Cyc and R1 are defined as above, in the presence of reduction equivalents. The compound of formula (II) is enzymatically reduced to the compound of formula (I) and the reduction equivalents consumed during reaction are regenerated by reacting a sacrificial alcohol to the corresponding sacrificial ketone using enzyme (E) and at least partially removing the sacrificial ketone from the reaction medium, and then isolating the product (I) so produced.

    摘要翻译： 本发明涉及一种制备式（I）的光学活性链烷醇的方法，其中n为0-5的整数; Cyc表示任选取代的单核或多核，饱和或不饱和的碳环或杂环，R 1表示卤素，SH，OH，NO 2，NR 2/3/3或3/3/3/3/4/4 / 其中R 2，R 3和R 4独立地表示H或低级烷基或低级烷氧基，X - 表示抗衡离子。 根据本发明，将选自脱氢酶，醛还原酶和羰基还原酶的酶（E）在含有式（II）的烷酮的培养基中温育，其中n，Cyc和R 1 如上所述，在还原当量的存在下。 将式（II）化合物酶还原成式（I）化合物，并且通过使用酶（E）使牺牲醇与相应的牺牲酮反应，并且至少部分除去牺牲酮来再生反应期间消耗的还原当量 从反应介质中分离出如此制备的产物（I）。