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    Lipase Production Method
    8.
    发明申请
    Lipase Production Method 审中-公开
    脂肪酶生产方法

    公开(公告)号：US20090104657A1

    公开(公告)日：2009-04-23

    申请号：US11922176

    申请日：2006-06-13

    申请人： Anke Beselin ,  Frank Rosenau ,  Karl-Erich Jaeger ,  Michael Breuer ,  Bernhard Hauer

    发明人： Anke Beselin ,  Frank Rosenau ,  Karl-Erich Jaeger ,  Michael Breuer ,  Bernhard Hauer

    IPC分类号： C12P21/00 ,  C07K14/00 ,  C12N9/48 ,  C07H21/00 ,  C12N15/63 ,  C12N1/21

    CPC分类号： C12N9/52 ,  C12N9/20

    摘要： The invention relates to novel proteins, especially with protease activity, which promote the production of extracellular lipases by bacteria, especially of the genus Burkholderia, nucleic acid sequences encoding them, expression constructs containing them, hosts transformed with them, methods for the production of the proteins with protease activity and methods for the production of lipases, especially by bacteria of the genus Burkholderia.

    摘要翻译： 本发明涉及特别是蛋白酶活性的新型蛋白质，其促进细菌特别是伯克霍尔德杆菌属细菌细胞外脂肪酶的生产，编码它们的核酸序列，含有它们的表达构建体，用它们转化的宿主，用于生产 具有蛋白酶活性的蛋白质和生产脂肪酶的方法，特别是伯克霍尔德氏菌属的细菌。

    Enzymatic Reduction for Producing Optically Active Alcohols
    9.
    发明申请
    Enzymatic Reduction for Producing Optically Active Alcohols 有权
    用于生产光活性醇的酶减量

    公开(公告)号：US20080318288A1

    公开(公告)日：2008-12-25

    申请号：US12158390

    申请日：2006-12-13

    申请人： Rainer Stürmer ,  Maria Kesseler ,  Bernhard Hauer ,  Thomas Friedrich ,  Michael Breuer ,  Hartwig Schröder

    发明人： Rainer Stürmer ,  Maria Kesseler ,  Bernhard Hauer ,  Thomas Friedrich ,  Michael Breuer ,  Hartwig Schröder

    IPC分类号： C12P17/00 ,  C12N9/14

    CPC分类号： C07D333/16 ,  C07D333/20 ,  C12P7/02 ,  C12P11/00 ,  C12P41/002

    摘要： A process for preparing optically active alkanols of the formula I in which n is an integer from 0 to 5; Cyc is an optionally substituted, mono- or polynuclear, saturated or unsaturated, carbocyclic or heterocyclic ring, and R1 is halogen, SH, OH, NO2, NR2R3 or NR2R3R4+X−, with R2, R3 and R4 independently of one another being hydrogen or a lower alkyl or lower alkoxy radical and X− being a counterion, which process comprises incubating in a medium comprising alkanone of the formula II in which n, Cyc and R1 are as defined above, an enzyme having a polypeptide sequence (i) SEQ ID NO: 1 or (ii) in which, compared to SEQ ID NO:1, up to 25% of the amino acid radicals have been altered by deletion, insertion, substitution or a combination thereof and which retains at least 50% of the enzymic activity of SEQ ID NO:1, with the compound of the formula II being enzymically reduced to give the compound of the formula I, and isolating the product formed.

    摘要翻译： 一种制备式I的光学活性链烷醇的方法，其中n为0至5的整数; Cyc是任选取代的单或多核饱和或不饱和的碳环或杂环，R1是卤素，SH，OH，NO2，NR2R3或NR2R3R4 + X-，R2，R3和R4彼此独立地是氢 或低级烷基或低级烷氧基，X是抗衡离子，该方法包括在包含式II的烷酮的培养基中孵育，其中n，Cyc和R 1如上定义，具有多肽序列（i）SEQ ID NO： SEQ ID NO：1或（ii）其中与SEQ ID NO：1相比，通过缺失，插入，取代或其组合改变多达25％的氨基酸基团，并且其保留至少50％的 酶活性为SEQ ID NO：1，其中式II化合物被酶还原，得到式I化合物，并分离形成的产物。