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    Enzymatic reduction for producing optically active alcohols
    1.
    发明授权
    Enzymatic reduction for producing optically active alcohols 有权
    用于制备光学活性醇的酶还原

    公开(公告)号：US07888080B2

    公开(公告)日：2011-02-15

    申请号：US12158390

    申请日：2006-12-13

    申请人： Rainer Stürmer ,  Maria Keβeler ,  Bernhard Hauer ,  Thomas Friedrich ,  Michael Breuer ,  Hartwig Schröder

    发明人： Rainer Stürmer ,  Maria Keβeler ,  Bernhard Hauer ,  Thomas Friedrich ,  Michael Breuer ,  Hartwig Schröder

    IPC分类号： C12P17/00 ,  C12N9/14

    CPC分类号： C07D333/16 ,  C07D333/20 ,  C12P7/02 ,  C12P11/00 ,  C12P41/002

    摘要： A process for preparing optically active alkanols of the formula I in which n is an integer from 0 to 5; Cyc is an optionally substituted, mono- or polynuclear, saturated or unsaturated, carbocyclic or heterocyclic ring, and R1 is halogen, SH, OH, NO2, NR2R3 or NR2R3R4+X−, with R2, R3 and R4 independently of one another being hydrogen or a lower alkyl or lower alkoxy radical and X− being a counterion, which process comprises incubating in a medium comprising alkanone of the formula II  in which n, Cyc and R1 are as defined above, an enzyme having a polypeptide sequence (i) SEQ ID NO: 1 or (ii) in which, compared to SEQ ID NO:1, up to 25% of the amino acid radicals have been altered by deletion, insertion, substitution or a combination thereof and which retains at least 50% of the enzymic activity of SEQ ID NO:1, with the compound of the formula II being enzymically reduced to give the compound of the formula I, and isolating the product formed.

    摘要翻译： 一种制备式I的光学活性链烷醇的方法，其中n为0至5的整数; Cyc是任选取代的单或多核饱和或不饱和的碳环或杂环，R1是卤素，SH，OH，NO2，NR2R3或NR2R3R4 + X-，R2，R3和R4彼此独立地是氢 或低级烷基或低级烷氧基，X是抗衡离子，该方法包括在包含式II的烷酮的培养基中孵育，其中n，Cyc和R 1如上定义，具有多肽序列（i）SEQ ID NO： SEQ ID NO：1或（ii）其中与SEQ ID NO：1相比，通过缺失，插入，取代或其组合改变多达25％的氨基酸基团，并且其保留至少50％的 酶活性为SEQ ID NO：1，其中式II化合物被酶还原，得到式I化合物，并分离形成的产物。

    Method for producing optically active alcohols from alkanones using a dehydrogenase of azoarcus
    3.
    发明授权
    Method for producing optically active alcohols from alkanones using a dehydrogenase of azoarcus 有权
    使用azoarcus脱氢酶从链烷醇生产光学活性醇的方法

    公开(公告)号：US07785847B2

    公开(公告)日：2010-08-31

    申请号：US11579292

    申请日：2005-05-04

    申请人： Rainer Stürmer ,  Maria Keβeler ,  Bernhard Haur ,  Thomas Friedrich ,  Michael Breuer ,  Hartwig Schröder

    发明人： Rainer Stürmer ,  Maria Keβeler ,  Bernhard Haur ,  Thomas Friedrich ,  Michael Breuer ,  Hartwig Schröder

    IPC分类号： C12P7/22 ,  C12P7/04 ,  C12P17/00 ,  C12N9/04 ,  C12N9/14 ,  C12N1/20 ,  C07H21/04

    CPC分类号： C07D333/22 ,  C07D333/16 ,  C12P17/00

    摘要： The invention relates to a method for producing the optically active alkanols of formula (I), wherein n is an integer of from 0 to 5; Cyc represents an optionally substituted, mononuclear or polynuclear, saturated or unsaturated, carbocylic or heterocyclic ring, and R1 represents halogen, SH, OH, NO2, NR2R3 or NR2R3R4+X−, wherein R2, R3 and R4 independently represent H or a lower alkyl or lower alkoxy group and X− represents a counterion. According to the invention, an enzyme (E) selected from the groups of dehydrogenases, aldehyde reductases and carbonyl reductases is incubated in a medium containing the alkanone of formula (II), wherein n, Cyc and R1 are defined as above, in the presence of reduction equivalents. The compound of formula (II) is enzymatically reduced to the compound of formula (I) and the reduction equivalents consumed during reaction are regenerated by reacting a sacrificial alcohol to the corresponding sacrificial ketone using enzyme (E) and at least partially removing the sacrificial ketone from the reaction medium, and then isolating the product (I) so produced.

    摘要翻译： 本发明涉及一种制备式（I）的光学活性链烷醇的方法，其中n为0-5的整数; Cyc表示任选取代的单核或多核，饱和或不饱和的碳环或杂环，并且R 1表示卤素，SH，OH，NO 2，NR 2 R 3或NR 2 R 3 R 4 + X - ，其中R 2，R 3和R 4独立地表示H或低级烷基 或低级烷氧基，X-表示抗衡离子。 根据本发明，在含有式（II）的烷酮的培养基中温育选自脱氢酶，醛还原酶和羰基还原酶的酶的酶（E），其中n，R 1和R 1如上定义，在存在下 的还原当量。 将式（II）化合物酶还原成式（I）化合物，并且通过使用酶（E）使牺牲醇与相应的牺牲酮反应，并且至少部分除去牺牲酮来再生反应期间消耗的还原当量 从反应介质中分离出如此制备的产物（I）。

    Enzymatic Reduction for Producing Optically Active Alcohols
    4.
    发明申请
    Enzymatic Reduction for Producing Optically Active Alcohols 有权
    用于生产光活性醇的酶减量

    公开(公告)号：US20080318288A1

    公开(公告)日：2008-12-25

    申请号：US12158390

    申请日：2006-12-13

    申请人： Rainer Stürmer ,  Maria Kesseler ,  Bernhard Hauer ,  Thomas Friedrich ,  Michael Breuer ,  Hartwig Schröder

    发明人： Rainer Stürmer ,  Maria Kesseler ,  Bernhard Hauer ,  Thomas Friedrich ,  Michael Breuer ,  Hartwig Schröder

    IPC分类号： C12P17/00 ,  C12N9/14

    CPC分类号： C07D333/16 ,  C07D333/20 ,  C12P7/02 ,  C12P11/00 ,  C12P41/002

    摘要： A process for preparing optically active alkanols of the formula I in which n is an integer from 0 to 5; Cyc is an optionally substituted, mono- or polynuclear, saturated or unsaturated, carbocyclic or heterocyclic ring, and R1 is halogen, SH, OH, NO2, NR2R3 or NR2R3R4+X−, with R2, R3 and R4 independently of one another being hydrogen or a lower alkyl or lower alkoxy radical and X− being a counterion, which process comprises incubating in a medium comprising alkanone of the formula II in which n, Cyc and R1 are as defined above, an enzyme having a polypeptide sequence (i) SEQ ID NO: 1 or (ii) in which, compared to SEQ ID NO:1, up to 25% of the amino acid radicals have been altered by deletion, insertion, substitution or a combination thereof and which retains at least 50% of the enzymic activity of SEQ ID NO:1, with the compound of the formula II being enzymically reduced to give the compound of the formula I, and isolating the product formed.

    摘要翻译： 一种制备式I的光学活性链烷醇的方法，其中n为0至5的整数; Cyc是任选取代的单或多核饱和或不饱和的碳环或杂环，R1是卤素，SH，OH，NO2，NR2R3或NR2R3R4 + X-，R2，R3和R4彼此独立地是氢 或低级烷基或低级烷氧基，X是抗衡离子，该方法包括在包含式II的烷酮的培养基中孵育，其中n，Cyc和R 1如上定义，具有多肽序列（i）SEQ ID NO： SEQ ID NO：1或（ii）其中与SEQ ID NO：1相比，通过缺失，插入，取代或其组合改变多达25％的氨基酸基团，并且其保留至少50％的 酶活性为SEQ ID NO：1，其中式II化合物被酶还原，得到式I化合物，并分离形成的产物。

    Modified nitrilases and their use in methods for the production of carboxylic acids
    5.
    发明授权
    Modified nitrilases and their use in methods for the production of carboxylic acids 有权
    改性腈水解酶及其在羧酸生产方法中的应用

    公开(公告)号：US07985572B2

    公开(公告)日：2011-07-26

    申请号：US10546611

    申请日：2004-02-24

    申请人： Thomas Zelinski ,  Maria Keβeler ,  Bernhard Hauer ,  Thomas Friedrich

    发明人： Thomas Zelinski ,  Maria Keβeler ,  Bernhard Hauer ,  Thomas Friedrich

    IPC分类号： C12N9/00 ,  C12N9/78 ,  C12N1/20 ,  C12N15/00

    CPC分类号： C12N9/80 ,  C12N9/78 ,  C12N15/8274 ,  C12P7/40 ,  C12P7/42

    摘要： The present Invention relates to new nitrilases with modified Substrate acceptance, the method to obtain them and the use of the aforesaid nitrilases. Said nitrilases are encoded by a polypeptide sequence, which at a Position corresponding to Position 296 in wild-type Alcaligenes faecalis comprises an amino acid which is not tyrosine. The Invention further relates to nucleic acid sequences and amino acid sequences encoding Said nitrilases, expression constructs comprising Said nucleic acid sequences, vectors comprising the nucleic acid sequences or the exPression constructs, to organisms, preferably microorganisms, comprising the nucleic acid sequences, expression constructs or vectors. The Invention additionally relates to a method for preparing carboxylic acids, preferably substituted chiral carboxylic acids from racemic nitriles.

    摘要翻译： 本发明涉及具有改进的基板接受性的新腈水解酶，获得它们的方法和使用上述腈水解酶。 所述腈水解酶由多肽序列编码，其在与野生型产碱杆菌粪便中的位置296相对应的位置包含不是酪氨酸的氨基酸。 本发明还涉及编码所述腈水解酶的核酸序列和氨基酸序列，包含所述核酸序列，包含核酸序列或exPression构建体的载体的表达构建体，包含核酸序列，表达构建体或生物体 向量 本发明还涉及从外消旋腈制备羧酸，优选取代的手性羧酸的方法。

    METHOD FOR THE ENZYMATIC REDUCTION OF ALPHA- AND BETA-DEHYDROAMINO ACIDS USING ENOATE REDUCTASES
    9.
    发明申请
    METHOD FOR THE ENZYMATIC REDUCTION OF ALPHA- AND BETA-DEHYDROAMINO ACIDS USING ENOATE REDUCTASES 审中-公开
    使用降低氧化还原酶抑制阿尔法和β-去氢氨基酸的方法

    公开(公告)号：US20100304448A1

    公开(公告)日：2010-12-02

    申请号：US12746973

    申请日：2008-12-08

    申请人： Rainer Stürmer ,  Bernhard Hauer ,  Thomas Friedrich ,  Kurt Faber ,  Melanie Hall ,  Clemens Stückler

    发明人： Rainer Stürmer ,  Bernhard Hauer ,  Thomas Friedrich ,  Kurt Faber ,  Melanie Hall ,  Clemens Stückler

    IPC分类号： C12P13/04

    CPC分类号： C12P13/04

    摘要： A method for the enzymatic preparation of amino acids of the general formula (3) or (4) from alpha-dehydroamino acids of the general formula (1) or (2) wherein R1, R2 are independently of one another H, C1-C6 alkyl, C2-C6 alkenyl, an optionally substituted carbo- or heterocyclic, aromatic or nonaromatic radical, or an alkylaryl radical, or a carboxyl radical (—COOR), R3 is H, formyl, acetyl, propionyl, benzyl, benzyloxycarbonyl, BOC, Alloc, R is H, C1-C6 alkyl, aryl, by reducing a compound of the formula (1) or (2) in the presence of a reductase.

    摘要翻译： 由通式（1）或（2）的α-脱氢氨基酸酶制备通式（3）或（4）的氨基酸的方法，其中R 1，R 2彼此独立地为H，C 1 -C 6 烷基，C 2 -C 6烯基，任选取代的碳 - 或杂环芳族或非芳族基团，或烷基芳基或羧基（-COOR），R 3为H，甲酰基，乙酰基，丙酰基，苄基，苄氧基羰基， 通过在还原酶的存在下还原式（1）或（2）的化合物，R分子是H，C 1 -C 6烷基，芳基。

    Butynol I esterase
    10.
    发明申请
    Butynol I esterase 有权
    丁醇I酯酶

    公开(公告)号：US20100196970A1

    公开(公告)日：2010-08-05

    申请号：US12422785

    申请日：2009-04-13

    申请人： Bernhard Hauer ,  Thomas Friedrich ,  Christoph Nübling ,  Rainer Stürmer ,  Wolfgang Ladner

    发明人： Bernhard Hauer ,  Thomas Friedrich ,  Christoph Nübling ,  Rainer Stürmer ,  Wolfgang Ladner

    IPC分类号： C12P7/00

    CPC分类号： C12N9/16 ,  C12P41/00

    摘要： The invention relates to novel proteins from Pseudomonas glumae, having esterase activity, in particular butynol I esterase activity, to nucleic acid sequences coding therefor, to expression cassettes, vectors and recombinant microorganisms; to methods for preparing said proteins and to the use thereof for enzymic, in particular enantioselective enzymic, ester hydrolysis or transesterification of organic esters.

    摘要翻译： 本发明涉及具有酯酶活性，特别是丁酸酯酶活性的编码的核酸序列，表达盒，载体和重组微生物的新型蛋白质， 涉及用于制备所述蛋白质的方法及其用于酶，特别是对映选择性酶，酯水解或酯交换有机酯的用途。