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公开(公告)号：US20080039620A1
公开(公告)日：2008-02-14
申请号：US10590268
申请日：2005-02-10
申请人： Mitsuo Sekine , Kohji Seio , Akihiro Ohkubo
发明人： Mitsuo Sekine , Kohji Seio , Akihiro Ohkubo
CPC分类号： C07F9/65616 , C07F9/65586 , C07H21/04 , Y02P20/55
摘要： The purpose of the present invention is therefore to provide a method for binding a 3′-end nucleoside unit comprising any base to a hydroxyl group on a solid-phase support under completely the same condition as in DNA chain elongation reaction. The present invention relates to a 3′-end nucleoside unit comprising phosphoramidite that is a compound represented by the following formula: (N)—O—(R1)Si(R2)-(C6H4)—(CH2)n-O—P(OR3)N(R4)(R5) (I) wherein (N) represents any nucleoside or its derivative, each of R1, R2, R4 and R5 is an alkyl or aryl group, R3 is a phosphate-protecting group, and n is an integer of from 1 to 5; a solid-phase support having said 3′-end nucleoside unit; and a method for the synthesis of a nucleic acid oligomer with the use of said solid-phase support.
摘要翻译： 因此，本发明的目的是提供一种在与DNA链伸长反应完全相同的条件下将包含任何碱基的3'-末端核苷单元与固相载体上的羟基结合的方法。 本发明涉及包含亚磷酰胺的3'-末端核苷单位，该亚磷酰胺是下式表示的化合物：＆lt;＆lt;线内式记载=“在线式”末端=“铅”→（N） - O-（R 1）Si（R 2） - （C 6 H 4） - （CH 2）nOP（OR 3）N（R 4） 其中（N）表示任何核苷或其衍生物，R 1，R 2，R 4和R 5各自为 烷基或芳基，R3是磷酸酯保护基，n是1至5的整数; 具有所述3'-末端核苷单元的固相载体; 以及使用所述固相载体合成核酸寡聚物的方法。
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12.发明申请Electrochemically active ligand for sequence-specific detection of double-stranded nucleic acid molecule 失效用于双链核酸分子的序列特异性检测的电化学活性配体公开(公告)号：US20070172826A1
公开(公告)日：2007-07-26
申请号：US10590232
申请日：2005-02-23
申请人： Mitsuo Sekine , Kohji Seio , Masahiro Mizuta , Takeshi Terada
发明人： Mitsuo Sekine , Kohji Seio , Masahiro Mizuta , Takeshi Terada
CPC分类号： C07D403/14 , C07F17/02 , C12Q1/6818
摘要： The purpose of the present invention is to provide a compound that specifically binds to the base sequence of a double-stranded nucleic acid molecule. The compound can reduce the electrochemical signal/noise ratio (S/N) in electrochemical detection, and as a result, the detection sensitivity (precision) will be greatly improved so as to enable the determination of an ultratrace amount of nucleic acid molecule. The present invention relates to a ferrocene compound represented by the following general formula (I): wherein “A” represents a divalent ferrocene-containing linker or ferrocene-1,1′-yl, R2 represents a hydrogen atom or alkyl; “n” and “m” represent any natural numbers; and “V” and “X” represent the pyrrole-imidazole-polyamide (PIPA); to a ligand consisting of said ferrocene compound for sequence-specific detection of double-stranded nucleic acid molecules; to a method for electrochemical detection of double-stranded nucleic acid molecules 8 with the use of said ligand; and to an apparatus or device for the electrochemical detection with the use of said ligand.
摘要翻译： 本发明的目的是提供特异性结合双链核酸分子的碱基序列的化合物。 该化合物可以降低电化学检测中的电化学信噪比（S / N），结果检测灵敏度（精度）将大大提高，从而能够测定超临界量的核酸分子。 本发明涉及由以下通式（I）表示的二茂铁化合物：其中“A”表示含二茂铁的连接体或二茂铁-1,1'-基，R 2表示 氢原子或烷基; “n”和“m”表示任何自然数; “V”和“X”表示吡咯 - 咪唑 - 聚酰胺（PIPA）。 涉及由所述二茂铁化合物组成的配体，用于双链核酸分子的序列特异性检测; 涉及使用所述配体进行电泳检测双链核酸分子8的方法; 以及使用所述配体进行电化学检测的装置或装置。
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公开(公告)号：US4280210A
公开(公告)日：1981-07-21
申请号：US948633
申请日：1978-10-03
申请人： Yasuhiko Nishikubo , Mitsuo Sekine
发明人： Yasuhiko Nishikubo , Mitsuo Sekine
CPC分类号： G04G13/021
摘要： An electronic alarm timepiece comprises a quartz crystal oscillator, a frequency divider, a display driving means, a display means, an alarm signal shaping means and an alarm means. The alarm signal shaping means is so arranged that a pair of output signals which are opposite in phase are produced with the aid of output signals of the frequency divider for actuating the alarm means, whereby it is possible to provide an alarm signal shaping means in the form of a complementary metal oxide semiconductor (CMOS) integrated circuit having sufficient power to actuate the alarm means without other amplifying circuits.
摘要翻译： 电子闹钟包括石英晶体振荡器，分频器，显示驱动装置，显示装置，报警信号整形装置和报警装置。 报警信号整形装置被布置成借助于用于启动报警装置的分频器的输出信号产生相位相反的一对输出信号，从而可以在报警信号整形装置中提供报警信号整形装置 具有足够功率的互补金属氧化物半导体（CMOS）集成电路的形式，以在没有其它放大电路的情况下致动报警装置。
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公开(公告)号：US4225824A
公开(公告)日：1980-09-30
申请号：US915739
申请日：1978-06-15
申请人： Mitsuo Sekine , Sizuo Yamaguchi
发明人： Mitsuo Sekine , Sizuo Yamaguchi
CPC分类号： G04G3/022
摘要： A frequency adjusting circuit for digitally adjusting the frequency of a time reference signal consisting of a series of pulses is disclosed. The circuit function to adjust the frequency of said series of pulses by subtracting or adding a given number of pulses from or to said series of pulses for a time set beforehand every time upon receipt of a predetermined number of pulses of said time reference signal.
摘要翻译： 公开了一种用于数字调节由一系列脉冲组成的时间基准信号的频率的频率调节电路。 所述电路功能通过从接收到所述时间参考信号的预定数量的脉冲而每次预先设定的时间减去或添加给所述一系列脉冲的给定数量的脉冲来调整所述一系列脉冲的频率。
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公开(公告)号：USD597027S1
公开(公告)日：2009-07-28
申请号：US29288542
申请日：2007-06-14
申请人： Yoshihiro Tanabe , Fumio Morikawa , Mitsuo Sekine
设计人： Yoshihiro Tanabe , Fumio Morikawa , Mitsuo Sekine
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公开(公告)号：US4507572A
公开(公告)日：1985-03-26
申请号：US618771
申请日：1984-06-11
申请人： Shingo Hashimoto , Mitsuo Sekine
发明人： Shingo Hashimoto , Mitsuo Sekine
CPC分类号： H03K5/2481 , G05F3/262
摘要： A voltage sensing circuit is disclosed, which utilizes a reference voltage generating circuit for producing a reference voltage whose value is determined as a function of absolute temperature, independent of circuit element variations, the reference voltage being generated by means of a pair of MOS field effect transistors having different values of Id/K, where Id is drain current and K is the channel pattern ratio W/L.
摘要翻译： 公开了一种电压检测电路，其利用参考电压产生电路来产生参考电压，该参考电压的值被确定为绝对温度的函数，与电路元件变化无关，参考电压是通过一对MOS场效应产生的 晶体管具有不同的Id / K值，其中Id是漏极电流，K是沟道图案比W / L。
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公开(公告)号：US4448549A
公开(公告)日：1984-05-15
申请号：US347750
申请日：1982-02-09
申请人： Shingo Hashimoto , Mitsuo Sekine
发明人： Shingo Hashimoto , Mitsuo Sekine
摘要： A temperature sensing device is disclosed which utilizes a temperature sensing circuit which generates a voltage whose level varies linearly in accordance with temperature, this voltage being produced using a pair of MOS field effect transistors which have different values of the ratio Id/K, where Id is drain current and K is the channel pattern ratio of channel width to channel length.
摘要翻译： 公开了一种温度感测装置，其利用温度感测电路，其产生电平，其电平根据温度线性变化，该电压使用具有不同值Id / K的一对MOS场效应晶体管产生，其中Id 是漏极电流，K是沟道宽度与沟道长度的沟道图案比。
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公开(公告)号：US4240154A
公开(公告)日：1980-12-16
申请号：US920443
申请日：1978-06-29
申请人： Mitsuo Sekine
发明人： Mitsuo Sekine
CPC分类号： G04G13/023
摘要： An electronic digital stop watch including means for generating a time standard signal, a display device for indicating the time lapsed from the start operation and alarm control means provided for presetting a desired alarm time and for generating an audible alarm signal when the lapsed time becomes equal to the preset time.
摘要翻译： 一种电子数字秒表，包括用于产生时间标准信号的装置，用于指示从开始操作过去的时间的显示装置和用于预设所需的报警时间的报警控制装置，以及当经过的时间变得相等时产生可听见的报警信号 到预设时间。
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公开(公告)号：US4173758A
公开(公告)日：1979-11-06
申请号：US822355
申请日：1977-08-05
申请人： Mitsuo Sekine
发明人： Mitsuo Sekine
CPC分类号： G04G9/0047 , G09G3/16
摘要： In a driving circuit for an electrochromic display device of the type wherein a clock signal is applied to the common electrode segment, and a driving signal having a different level from that of the clock signal is applied to the display segment electrode in response to the clock signal and a display decoder output, thereby effecting display and termination thereof by using a single source, gate circuit means is provided for preventing short-circuiting of the electrodes caused by the delay time between the clock signal and the driving signal.
摘要翻译： 在这种类型的电致变色显示装置的驱动电路中，其中时钟信号被施加到公共电极段，并且具有与时钟信号不同的电平的驱动信号响应于时钟被施加到显示段电极 信号和显示解码器输出，从而通过使用单个源实现其显示和终止，提供了门电路装置，用于防止由时钟信号和驱动信号之间的延迟时间引起的电极的短路。
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公开(公告)号：US07397668B2
公开(公告)日：2008-07-08
申请号：US11561020
申请日：2006-11-17
申请人： Mitsuo Sekine , Yutaka Noguchi , Makoto Seo
发明人： Mitsuo Sekine , Yutaka Noguchi , Makoto Seo
CPC分类号： H01R9/2608 , H05K7/1465
摘要： A signal input/output device includes an array of signal input/output units. The signal input/output units have respective bases, each of which includes a connecting member. The connecting member has a second protruding end finger swingably supported on a base, and a first protruding end finger that engages with a stop of an adjacent base. The connecting member is positioned and fixed (secured) by a screw, which extends through a bottom plate and is threaded in a square nut. The connecting member, when secured in this manner, joins the adjacent bases to each other.
摘要翻译： 信号输入/输出装置包括信号输入/输出单元阵列。 信号输入/输出单元具有各自的基座，每个基座包括连接构件。 连接构件具有可摆动地支撑在基座上的第二突出端指，以及与相邻基座的止动件接合的第一突出端指。 连接构件通过螺钉定位和固定（固定），该螺钉延伸穿过底板并且拧入方形螺母。 当以这种方式固定时，连接构件将相邻的基座相互连接。
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