公开(公告)号：US20100112572A1

公开(公告)日：2010-05-06

申请号：US12507016

申请日：2009-07-21

申请人： Timothy M. Woudenberg ,  Dar Bahatt ,  Muhammad A. Sharaf ,  Timothy Z. Liu ,  Serguei Ermakov ,  Charles R. Connell ,  Jens J. Hyldig-Nielsen ,  Benjamin Schroeder ,  Paolo Vatta

发明人： Timothy M. Woudenberg ,  Dar Bahatt ,  Muhammad A. Sharaf ,  Timothy Z. Liu ,  Serguei Ermakov ,  Charles R. Connell ,  Jens J. Hyldig-Nielsen ,  Benjamin Schroeder ,  Paolo Vatta

IPC分类号： C12Q1/68

CPC分类号： G01N33/533 ,  C12Q1/6816 ,  G01N33/532 ,  G01N2035/00158 ,  C12Q2565/102 ,  C12Q2563/179

摘要： The teachings herein generally relates to probes comprising fabricated coded molecular tags for detecting analytes. The teachings also relate to compositions, methods, and kits for fabricating coded molecular tags comprising a multiplicity or reporter groups in an ordered pattern.

摘要翻译： 本文的教导通常涉及包含用于检测分析物的制造的编码分子标签的探针。 该教导还涉及用于制造编码分子标签的组合物，方法和试剂盒，其包含有序图案中的多个或报告基团。

公开(公告)号：US20100105886A1

公开(公告)日：2010-04-29

申请号：US12507035

申请日：2009-07-21

申请人： Timothy M. Woudenberg ,  Dar Bahatt ,  Muhammad A. Sharaf ,  Timothy Z. Liu ,  Serguei Ermakov ,  Charles R. Connell

发明人： Timothy M. Woudenberg ,  Dar Bahatt ,  Muhammad A. Sharaf ,  Timothy Z. Liu ,  Serguei Ermakov ,  Charles R. Connell

IPC分类号： C07H21/04

CPC分类号： C12Q1/6816 ,  C12Q2565/1025 ,  C12Q2563/107

摘要： The present teachings generally relate to probes and probes sets for detecting analytes. The teachings also relates to compositions, methods, and kits for assembling probes comprising at least one coded molecular tag.

摘要翻译： 本教导通常涉及用于检测分析物的探针和探针组。 该教导还涉及用于组装包含至少一个编码分子标签的探针的组合物，方法和试剂盒。

公开(公告)号：US20100096548A1

公开(公告)日：2010-04-22

申请号：US12644864

申请日：2009-12-22

申请人： Muhammad A. Sharaf ,  Maria C. Roque-Biewer

发明人： Muhammad A. Sharaf ,  Maria C. Roque-Biewer

IPC分类号： G01F1/00

CPC分类号： G01N27/44726 ,  Y10T436/10

摘要： The invention relates to methods, compositions, and systems for calibrating a fluorescent polynucleotide separation apparatus. One aspect of the invention is multiple color calibration standards and their use. A multiple color calibration standard is a mixture of at least two polynucleotides of different length, wherein each of the polynucleotides is labeled with a spectrally distinct fluorescent dye. Another aspect of the invention is to produce total emission temporal profiles of multiple color calibration standards for use in calibrating fluorescent polynucleotide separation apparatus. The peaks corresponding to the fluorescently labeled polynucleotides in the total emission temporal profile may be detected using a peak detector that is driven by changes in the slopes of the total emission temporal profile. Calibration of fluorescent polynucleotide separation apparatus, with various embodiments of the methods of the invention, includes the step of identification of the labeled polynucleotides of the multiple color calibration standards. The process of spectral calibration of a fluorescent polynucleotide separation apparatus using a multiple color calibration standard may include the step of the estimating (extracting) of the dyes' reference spectra, using information from the peak detection process performed on the total emission temporal profile. Other aspects of the invention include systems for separating and detecting fluorescently labeled polynucleotides, wherein the system is designed for spectral calibration in accordance with the subject calibration methods employing multiple color calibration standards. Another aspect of the invention is methods and compositions for detecting the flow of electrical current through a separation channel of a fluorescent polynucleotide separation apparatus. These methods and compositions employ monitoring dyes. Monitoring dyes are fluorescent dyes that are spectrally distinct from the dye on the polynucleotide intended to convey genetic information, e.g., fluorescent polynucleotide sequencing reaction products.

公开(公告)号：US07198900B2

公开(公告)日：2007-04-03

申请号：US10652430

申请日：2003-08-29

申请人： Timothy M. Woudenberg ,  Dar Bahatt ,  Muhammad A. Sharaf ,  Timothy Z. Liu ,  Serguei Ermakov ,  Charles R. Connell ,  Jens J. Hyldig-Nielsen

发明人： Timothy M. Woudenberg ,  Dar Bahatt ,  Muhammad A. Sharaf ,  Timothy Z. Liu ,  Serguei Ermakov ,  Charles R. Connell ,  Jens J. Hyldig-Nielsen

IPC分类号： C12Q1/68 ,  C07H21/02 ,  C07H21/04

CPC分类号： C12Q1/6827 ,  C12Q1/6858 ,  C12Q2565/1025 ,  C12Q2563/185 ,  C12Q2535/131 ,  C12Q2563/107

摘要： The present invention generally relates to the detection of analytes, particularly biomolecules in samples. The invention also relates to compositions, methods, and kits for detecting the presence of analytes, typically in multiplex detection formats. The invention also relates to methods for determining the presence of at least one analyte in a sample, the methods employing employ single molecule detection techniques to individually detect at least one molecular complex or at least part of a molecular complex.

摘要翻译： 本发明一般涉及检测分析物，特别是样品中的生物分子。 本发明还涉及通常以多重检测形式检测分析物的存在的组合物，方法和试剂盒。 本发明还涉及用于确定样品中至少一种分析物的存在的方法，采用单分子检测技术单独检测至少一种分子复合物或至少部分分子复合物的方法。