-
公开(公告)号：US06821402B1
公开(公告)日：2004-11-23
申请号：US09154178
申请日：1998-09-16
IPC分类号： G01N2726
CPC分类号： G01N27/44726 , Y10T436/10
摘要： The invention relates to methods, compositions, and systems for calibrating a fluorescent polynucleotide separation apparatus. One aspect of the invention is multiple color calibration standards and their use. A multiple color calibration standard is a mixture of at least two polynucleotides of different length, wherein each of the polynucleotides is labeled with a spectrally distinct fluorescent dye. Another aspect of the invention is to produce total emission temporal profiles of multiple color calibration standards for use in calibrating fluorescent polynucleotide separation apparatus. The peaks corresponding to the fluorescently labeled polynucleotides in the total emission temporal profile may be detected using a peak detector that is driven by changes in the slopes of the total emission temporal profile. Calibration of fluorescent polynucleotide separation apparatus, with various embodiments of the methods of the invention, includes the step of identification of the labeled polynucleotides of the multiple color calibration standards. The process of spectral calibration of a fluorescent polynucleotide separation apparatus using a multiple color calibration standard may include the step of the estimating (extracting) of the dyes' reference spectra, using information from the peak detection process performed on the total emission temporal profile. Other aspects of the invention include systems for separating and detecting fluorescently labeled polynucleotides, wherein the system is designed for spectral calibration in accordance with the subject calibration methods employing multiple color calibration standards. Another aspect of the invention is methods and compositions for detecting the flow of electrical current through a separation channel of a fluorescent polynucleotide separation apparatus. These methods and compositions employ monitoring dyes. Monitoring dyes are fluorescent dyes that are spectrally distinct from the dye on the polynucleotide intended to convey genetic information, e.g., fluorescent polynucleotide sequencing reaction products.
-
公开(公告)号：US06991712B2
公开(公告)日：2006-01-31
申请号：US09927791
申请日：2001-08-10
CPC分类号： G01N27/44726 , Y10T436/10
摘要： The invention relates to methods, compositions, and systems for calibrating a fluorescent polynucleotide separation apparatus. One aspect of the invention is multiple color calibration standards and their use. A multiple color calibration standard is a mixture of at least two polynucleotides of different length, wherein each of the polynucleotides is labeled with a spectrally distinct fluorescent dye. Another aspect of the invention is to produce total emission temporal profiles of multiple color calibration standards for use in calibrating fluorescent polynucleotide separation apparatus. The peaks corresponding to the fluorescently labeled polynucleotides in the total emission temporal profile may be detected using a peak detector that is driven by changes in the slopes of the total emission temporal profile. Calibration of fluorescent polynucleotide separation apparatus, with various embodiments of the methods of the invention, includes the step of identification of the labeled polynucleotides of the multiple color calibration standards. The process of spectral calibration of a fluorescent polynucleotide separation apparatus using a multiple color calibration standard may include the step of the estimating (extracting) of the dyes' reference spectra, using information from the peak detection process performed on the total emission temporal profile. Other aspects of the invention include systems for separating and detecting fluorescently labeled polynucleotides, wherein the system is designed for spectral calibration in accordance with the subject calibration methods employing multiple color calibration standards. Another aspect of the invention is methods and compositions for detecting the flow of electrical current through a separation channel of a fluorescent polynucleotide separation apparatus. These methods and compositions employ monitoring dyes. Monitoring dyes are fluorescent dyes that are spectrally distinct from the dye on the polynucleotide intended to convey genetic information, e.g., fluorescent polynucleotide sequencing reaction products.
-
公开(公告)号：US20100096548A1
公开(公告)日：2010-04-22
申请号：US12644864
申请日：2009-12-22
IPC分类号： G01F1/00
CPC分类号： G01N27/44726 , Y10T436/10
摘要： The invention relates to methods, compositions, and systems for calibrating a fluorescent polynucleotide separation apparatus. One aspect of the invention is multiple color calibration standards and their use. A multiple color calibration standard is a mixture of at least two polynucleotides of different length, wherein each of the polynucleotides is labeled with a spectrally distinct fluorescent dye. Another aspect of the invention is to produce total emission temporal profiles of multiple color calibration standards for use in calibrating fluorescent polynucleotide separation apparatus. The peaks corresponding to the fluorescently labeled polynucleotides in the total emission temporal profile may be detected using a peak detector that is driven by changes in the slopes of the total emission temporal profile. Calibration of fluorescent polynucleotide separation apparatus, with various embodiments of the methods of the invention, includes the step of identification of the labeled polynucleotides of the multiple color calibration standards. The process of spectral calibration of a fluorescent polynucleotide separation apparatus using a multiple color calibration standard may include the step of the estimating (extracting) of the dyes' reference spectra, using information from the peak detection process performed on the total emission temporal profile. Other aspects of the invention include systems for separating and detecting fluorescently labeled polynucleotides, wherein the system is designed for spectral calibration in accordance with the subject calibration methods employing multiple color calibration standards. Another aspect of the invention is methods and compositions for detecting the flow of electrical current through a separation channel of a fluorescent polynucleotide separation apparatus. These methods and compositions employ monitoring dyes. Monitoring dyes are fluorescent dyes that are spectrally distinct from the dye on the polynucleotide intended to convey genetic information, e.g., fluorescent polynucleotide sequencing reaction products.
-
公开(公告)号：US20110223596A1
公开(公告)日：2011-09-15
申请号：US13030622
申请日：2011-02-18
申请人： Timothy M. WOUDENBERG , Dar Bahatt , Muhammad A. Sharaf , Timothy Z. Liu , Serguei Ermakov , Charles R. Connell , Jens J. Hyldig-Nielsen
发明人： Timothy M. WOUDENBERG , Dar Bahatt , Muhammad A. Sharaf , Timothy Z. Liu , Serguei Ermakov , Charles R. Connell , Jens J. Hyldig-Nielsen
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6827 , C12Q1/6858 , C12Q2565/1025 , C12Q2563/185 , C12Q2535/131 , C12Q2563/107
摘要： The present invention generally relates to the detection of analytes, particularly biomolecules in samples. The invention also relates to compositions, methods, and kits for detecting the presence of analytes, typically in multiplex detection formats. The invention also relates to methods for determining the presence of at least one analyte in a sample, the methods employing employ single molecule detection techniques to individually detect at least one molecular complex or at least part of a molecular complex.
摘要翻译： 本发明一般涉及检测分析物，特别是样品中的生物分子。 本发明还涉及通常以多重检测形式检测分析物的存在的组合物，方法和试剂盒。 本发明还涉及用于确定样品中至少一种分析物的存在的方法，采用单分子检测技术单独检测至少一种分子复合物或至少部分分子复合物的方法。
-
公开(公告)号：US20120316083A1
公开(公告)日：2012-12-13
申请号：US13590794
申请日：2012-08-21
CPC分类号： C12Q1/6825 , C12Q1/6816 , C12Q1/6823 , C12Q1/6827 , C12Q1/6832 , C12Q1/6876 , G01N27/44726 , G01N27/44791 , C12Q2565/631
摘要： The present disclosure relates to methods of identifying target nucleic acids by using coded molecules and its analysis by translocation through a nanopore. Generally, coded molecules are subject to a target polynucleotide dependent modification. The modified coded molecule is detected by isolating the modified coded molecules from the unmodified coded molecules prior to analysis through the nanopore or by detecting a change in the signal pattern of the coded molecule when analyzed through the nanopore.
摘要翻译： 本公开内容涉及通过使用编码分子鉴定靶核酸的方法及其通过纳米孔易位的分析。 通常，经编码的分子经受目标多核苷酸依赖性修饰。 通过在通过纳米孔分析之前将经修饰的编码分子与未修饰的编码分子分离，或者当通过纳米孔分析时，通过检测编码分子的信号图谱的变化来检测修饰的编码分子。
-
公开(公告)号：US20100291548A1
公开(公告)日：2010-11-18
申请号：US11685189
申请日：2007-03-12
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6825 , C12Q1/6816 , C12Q1/6823 , C12Q1/6827 , C12Q1/6832 , C12Q1/6876 , G01N27/44726 , G01N27/44791 , C12Q2565/631
摘要： The present disclosure relates to methods of identifying target nucleic acids by using coded molecules and its analysis by translocation through a nanopore. Generally, coded molecules are subject to a target polynucleotide dependent modification. The modified coded molecule is detected by isolating the modified coded molecules from the unmodified coded molecules prior to analysis through the nanopore or by detecting a change in the signal pattern of the coded molecule when analyzed through the nanopore.
摘要翻译： 本公开内容涉及通过使用编码分子鉴定靶核酸的方法及其通过纳米孔易位的分析。 通常，经编码的分子经受目标多核苷酸依赖性修饰。 通过在通过纳米孔分析之前将经修饰的编码分子与未修饰的编码分子分离，或者当通过纳米孔分析时，通过检测编码分子的信号图谱的变化来检测修饰的编码分子。
-
公开(公告)号：US20090280497A1
公开(公告)日：2009-11-12
申请号：US12493063
申请日：2009-06-26
申请人： Timothy M. Woudenberg , Dar Bahatt , Muhammad A. Sharaf , Timothy Z. Liu , Serguei Ermakov , Charles R. Connell , Jens J. Hyldig-Nielsen
发明人： Timothy M. Woudenberg , Dar Bahatt , Muhammad A. Sharaf , Timothy Z. Liu , Serguei Ermakov , Charles R. Connell , Jens J. Hyldig-Nielsen
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6827 , C12Q1/6858 , C12Q2565/1025 , C12Q2563/185 , C12Q2535/131 , C12Q2563/107
摘要： The present invention generally relates to the detection of analytes, particularly biomolecules in samples. The invention also relates to compositions, methods, and kits for detecting the presence of analytes, typically in multiplex detection formats. The invention also relates to methods for determining the presence of at least one analyte in a sample, the methods employing employ single molecule detection techniques to individually detect at least one molecular complex or at least part of a molecular complex.
摘要翻译： 本发明一般涉及检测分析物，特别是样品中的生物分子。 本发明还涉及通常以多重检测形式检测分析物的存在的组合物，方法和试剂盒。 本发明还涉及用于确定样品中至少一种分析物的存在的方法，采用单分子检测技术单独检测至少一种分子复合物或至少部分分子复合物的方法。
-
公开(公告)号：US06964848B2
公开(公告)日：2005-11-15
申请号：US09816150
申请日：2001-03-26
CPC分类号： C12Q1/6823 , C12Q2527/113 , C12Q2537/165 , C12Q2561/109 , C12Q2561/113
摘要： The invention relates to improved methods of detecting and characterizing polynucleotide sequences and variations in polynucleotide sequences. The invention further relates to a device that can be used to detect and characterize polynucleotide sequences and variations in polynucleotide sequences.
摘要翻译： 本发明涉及检测和表征多核苷酸序列和多核苷酸序列变异的改进方法。 本发明还涉及可用于检测和表征多核苷酸序列和多核苷酸序列变异的装置。
-
9.发明授权
公开(公告)号：US6015667A
公开(公告)日：2000-01-18
申请号：US659115
申请日：1996-06-03
申请人： Muhammad A. Sharaf
发明人： Muhammad A. Sharaf
IPC分类号： C12N15/09 , C12Q1/68 , G01N21/27 , G01N21/64 , G01N27/416 , G01N27/42 , G01N27/447 , G01N30/74 , G01N30/86 , G01N33/50 , G06F17/00 , C12P19/34
CPC分类号： G01N30/8665 , G01N2201/129
摘要： A signal processing method in a processor is provided for performing a multicomponent analysis of a signal resulting from a spectral response of a mixture comprising a plurality of spectrally resolvable molecular species. The method provides both a determination of a concentration estimate and a statistical confidence interval for each species. In the method, a data vector d is received from a multichannel detector, data vector d having a length n.sub.c, n.sub.c being the number of detector channels being monitored. A calibration matrix K having n.sub.c rows and n.sub.p columns is provided wherein n.sub.c is larger than n.sub.p, n.sub.p being the number of spectrally resolvable molecular species. Next, a concentration estimate vector c having length n.sub.p is determined. Finally, a confidence interval CI.sub.i for each of the elements of the concentration estimate vector is determined according to the expressionCI.sub.i =c.sub.i .+-.(varcovar(c.sub.ii)).sup.1/2 Q.sub.(cl,nf)where Q is the critical value of a statistical distribution for a given level of confidence, cl, and a given number of degrees of freedom n.sub.f where n.sub.f =n.sub.c -n.sub.p. The invention further includes a program storage device embodying the method; a DNA sequencing process employing the method; and, an apparatus for carrying out the method.
摘要翻译： 提供了一种处理器中的信号处理方法，用于对包含多个可光谱解析的分子物质的混合物的光谱响应产生的信号进行多组分分析。 该方法提供了每种物种的浓度估计和统计置信区间的确定。 在该方法中，从多通道检测器接收数据矢量d，数据矢量d的长度为nc，nc为被监视的检测器通道的数量。 提供了具有nc行和np列的校准矩阵K，其中nc大于np，np是可光谱解析的分子种类的数量。 接下来，确定长度为np的浓度估计矢量c。 最后，根据表达式Cii = ci +/-（varcovar（cii））+ E，fra 1/2 + EE Q（cl，nf）确定浓度估计向量的每个元素的置信区间CIi，其中Q是 nf = nc-np给定给定的置信度cl和给定数量的自由度nf的统计分布的临界值。 本发明还包括体现该方法的程序存储装置; 采用该方法的DNA测序方法; 以及用于执行该方法的装置。
-
10.发明申请Quantitative Calibration Method and System for Genetic Analysis Instrumentation 审中-公开遗传分析仪器的定量校准方法和系统公开(公告)号：US20100276580A1
公开(公告)日：2010-11-04
申请号：US12611892
申请日：2009-11-03
申请人： Muhammad A. Sharaf , Wanli Bi , Kenneth J. Livak
发明人： Muhammad A. Sharaf , Wanli Bi , Kenneth J. Livak
CPC分类号： G01N21/274 , G01N21/278 , G01N21/6428 , G01N21/6452
摘要： Aspects of the present invention provide a method and apparatus of generating a calibration matrix for a spectral detector instrument. A calibration plate contains one or more dye mixtures in each well of the calibration plate at known absolute concentration. From the calibration plate, aspects of the present invention are used to prepare a concentration matrix based on the dyes used in the assay and the different dye mixtures used in the calibration plate. An excitation source exposes the calibration plate causing the spectral species in each of the wells to fluoresce. The emission spectra for the different dye mixtures of dyes as gathered by the spectral detector instrument at different points in the range of spectra is used to generate a spectral matrix. Bilinear calibration is performed on the concentration matrix and the spectral matrix as to determine a calibration matrix relating spectra directly to absolute concentrations.
摘要翻译： 本发明的方面提供了一种生成用于光谱检测器仪器的校准矩阵的方法和装置。 校准板在已知的绝对浓度下在校准板的每个孔中含有一种或多种染料混合物。 根据校准板，本发明的各方面用于制备基于测定中使用的染料和校准板中使用的不同染料混合物的浓度基质。 激发源暴露校准板，导致每个孔中的光谱物质发荧光。 由光谱检测仪器在光谱范围内的不同点收集的不同染料染料混合物的发射光谱用于产生光谱矩阵。 对浓度矩阵和光谱矩阵进行双线性校准，以确定直接将光谱与绝对浓度相关的校准矩阵。
-
-
-
-
-
-
-
-
-
搜索结果
14 条记录 (耗时 0.025 秒)
