公开(公告)号：US11371029B2

公开(公告)日：2022-06-28

申请号：US16865744

申请日：2020-05-04

Applicant: Novozymes A/S

Inventor： Allan Svendsen ,  Rakhi Saikia ,  Sohel Dalal ,  Vikram Kjoeller Bhatia ,  Lone Baunsgaard

IPC: C12N9/20

Abstract: The invention relates to a method for generating a preparation of lipase with increased lipolytic activity comprising a step of altering one or more nucleotides in a polynucleotide comprising a first polynucleotide encoding a propeptide operationally linked to a second polynucleotide encoding a lipase, wherein the alteration of one or more nucleotides are made in the first polynucleotide and the alteration independently results in a substitution, an insertion or a deletion in the encoded propeptide amino acid sequence.

公开(公告)号：US10724019B2

公开(公告)日：2020-07-28

申请号：US16540180

申请日：2019-08-14

Applicant: NOVOZYMES A/S

Inventor： Rakhi Saikia ,  Geetha Hiremath Mendez ,  Sohel Dalal ,  Padmavathi Balumuri ,  Santhosh Vasu Mepadam ,  Lone Baunsgaard ,  Jesper Vind ,  Garry Paul Gippert ,  Kim Borch

IPC: C12N9/20 ,  C11D3/386

Abstract: The present invention relates to a lipase variant of a parent lipase, which variant has lipase activity, at least 75% but less than 100% sequence identity to SEQ ID NO: 3 and comprises a substitution at one or more positions corresponding to positions 1; 2; 3; 4; 5; 6; 7; 9; 10; 11; 12; 13; 14; 15; 17; 18; 29; 30; 33; 34; 36; 37; 38; 40; 41; 43; 44; 45; 46; 47; 49; 50; 51; 52; 54; 55; 57; 58; 59; 60; 69; 70; 71; 72; 81; 82; 85; 86; 87; 89; 90; 91; 92; 94; 95; 96; 97; 99; 100; 101; 102; 103; 105; 108; 111; 115; 116; 118; 119; 120; 122; 123; 124; 126; 127; 128; 129; 130; 131; 132; 133; 134; 136; 144; 152; 155; 157; 158; 159; 161; 162; 163; 177; 178; 181; 182; 184; 185; 187; 189; 191; 196; 197; 199; 203; 205; 206; 207; 208; 209; 211; 215; 220; 221; 222; 223; 224; 226; 229; 230; 232; 235; 236; 240; 242; 243; 244; 247; 248; 252; 254; 255; 257; 260; 261; 262 of SEQ ID NO: 3. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

公开(公告)号：US20180355330A1

公开(公告)日：2018-12-13

申请号：US16012906

申请日：2018-06-20

Applicant: NOVOZYMES A/S

Inventor： Padmavathi Balumuri ,  Rakhi Saikia ,  Allan Svendsen ,  Lone Baunsgaard

IPC: C12N9/20 ,  C12P7/64 ,  C12P21/06 ,  C12N15/09 ,  C11D3/386

CPC classification number: C12N9/20 ,  C11D3/38627 ,  C12N15/09 ,  C12P7/6418 ,  C12P21/06 ,  C12Y301/01003

Abstract: The present invention relates to a lipase variant of a parent lipase, which variant has lipase activity, at least 75% but less than 100% sequence identity to SEQ ID NO: 3 and comprises a substitution at one or more positions corresponding to positions 1; 2; 3; 4; 5; 6; 7; 9; 10; 11; 12; 16; 19; 30; 31; 34; 36; 37; 39; 40; 42; 44; 51; 52; 53; 54; 56; 58; 59; 70; 71; 72; 73; 83; 84; 86; 88; 90; 92; 93; 95; 96; 100; 101; 102; 104; 106; 109; 110; 112; 117; 119; 124; 125; 127; 128; 131; 132; 133; 134; 135; 137; 158; 159; 160; 161; 162; 163; 165; 166; 167; 168; 170; 181; 182; 183; 189; 190; 192; 194; 196; 202; 210; 211; 212; 220; 225; 227; 228; 229; 230; 231; 233; 237; 238; 239; 240; 242; 246; 247; 248; 252; 259; 262; 264; 269 of SEQ ID NO: 3. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

公开(公告)号：US20170152495A1

公开(公告)日：2017-06-01

申请号：US15310128

申请日：2015-05-26

Applicant: Novozymes A/S

Inventor： Padmavathi Balumuri ,  Rakhi Saikia ,  Allan Svendsen ,  Lone Baunsgaard

IPC: C12N9/20

CPC classification number: C12N9/20 ,  C11D3/38627 ,  C12N15/09 ,  C12P7/6418 ,  C12P21/06 ,  C12Y301/01003

Abstract: The present invention relates to a lipase variant of a parent lipase, which variant has lipase activity, at least 75% but less than 100% sequence identity to SEQ ID NO: 3 and comprises a substitution at one or more positions corresponding to positions 1; 2; 3; 4; 5; 6; 7; 9; 10; 11; 12; 16; 19; 30; 31; 34; 36; 37; 39; 40; 42; 44; 51; 52; 53; 54; 56; 58; 59; 70; 71; 72; 73; 83; 84; 86; 88; 90; 92; 93; 95; 96; 100; 101; 102; 104; 106; 109; 110; 112; 117; 119; 124; 125; 127; 128; 131; 132; 133; 134; 135; 137; 158; 159; 160; 161; 162; 163; 165; 166; 167; 168; 170; 181; 182; 183; 189; 190; 192; 194; 196; 202; 210; 211; 212; 220; 225; 227; 228; 229; 230; 231; 233; 237; 238; 239; 240; 242; 246; 247; 248; 252; 259; 262; 264; 269 of SEQ ID NO: 3. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

公开(公告)号：US20160355759A1

公开(公告)日：2016-12-08

申请号：US15101275

申请日：2014-12-10

Applicant: NOVOZYMES A/S

Inventor： Leonardo De Maria ,  Rakhi Saikia ,  Santhosh Vasu Mepadam ,  Ting Sun

IPC: C11D3/386 ,  D06M16/00 ,  C12N9/18 ,  C11D11/00

CPC classification number: C11D3/38636 ,  C11D11/0017 ,  C12N9/18 ,  C12Y301/01074 ,  D06M16/003 ,  D06M2101/32 ,  D06M2200/35

Abstract: Variants with cutinase activity of a parent cutinase, comprising an alteration at one or more (e.g. several) positions corresponding to positions: 181, 182, 115, 161, 1, 2, 43, 55, 79, or 5 of SEQ ID NO: 2, wherein the alteration is a substitution for positions 181, 115, 161, 43, 55, 79, and 5, and a deletion for positions 1, 2 and 182, and wherein the variant has at least 75% but less than 100% sequence identity to the mature polypeptide of SEQ ID NO: 2. Polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and method for obtaining and methods of producing the variants. Compositions comprising the variants and methods for using the variants.

Abstract translation: 包含亲本角质酶的角质酶活性的变体，包含对应于SEQ ID NO：1的位置：181,182,115,161,1,2,43,55,79或5的一个或多个（例如几个）位置的改变。 2，其中所述改变是位置181,151,161,43,55,79和5的替代，以及位置1,2和182的缺失，并且其中所述变体具有至少75％但小于100％ 与SEQ ID NO：2的成熟多肽的序列同一性。 核酸构建体，载体和包含多核苷酸的宿主细胞; 和获得的方法以及制备变体的方法。 包含变体的组合物和使用变体的方法。

公开(公告)号：US20230017381A1

公开(公告)日：2023-01-19

申请号：US17782721

申请日：2020-12-17

Applicant: Novozymes A/S

Inventor： Rakhi Saikia ,  Amol Vaijanathappa Shivange ,  Sohel Dalal ,  Kenneth Jensen ,  Lars Olsen ,  Kristian Bertel Romer M. Krogh ,  Madelyn Mallison Shoup ,  Bernardo Vidal, Jr.

IPC: C12N9/24 ,  C12P19/14 ,  C12P19/04

Abstract: The present invention relates to xylanase variants of a parent xylanase having increased thermostability when compared to the parent xylanase. The present invention also relates to polynucleotides encoding the variants, nucleic acid constructs, vectors, and host cells comprising the polynucleotides, and method of producing the variants of the present invention.

公开(公告)号：US20210130798A1

公开(公告)日：2021-05-06

申请号：US17146676

申请日：2021-01-12

Applicant: Novozymes A/S

Inventor： Padmavathi Balumuri ,  Rakhi Saikia ,  Allan Svendsen ,  Lone Baunsgaard

IPC: C12N9/20 ,  C12P21/06 ,  C11D3/386 ,  C12N15/09 ,  C12P7/64

Abstract: The present invention relates to a lipase variant of a parent lipase, which variant has lipase activity, at least 75% but less than 100% sequence identity to SEQ ID NO: 3 and comprises a substitution at one or more positions corresponding to positions 1; 2; 3; 4; 5; 6; 7; 9; 10; 11; 12; 16; 19; 30; 31; 34; 36; 37; 39; 40; 42; 44; 51; 52; 53; 54; 56; 58; 59; 70; 71; 72; 73; 83; 84; 86; 88; 90; 92; 93; 95; 96; 100; 101; 102; 104; 106; 109; 110; 112; 117; 119; 124; 125; 127; 128; 131; 132; 133; 134; 135; 137; 158; 159; 160; 161; 162; 163; 165; 166; 167; 168; 170; 181; 182; 183; 189; 190; 192; 194; 196; 202; 210; 211; 212; 220; 225; 227; 228; 229; 230; 231; 233; 237; 238; 239; 240; 242; 246; 247; 248; 252; 259; 262; 264; 269 of SEQ ID NO: 3. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

公开(公告)号：US20210130743A1

公开(公告)日：2021-05-06

申请号：US16605091

申请日：2018-04-26

Applicant: Novozymes A/S

Inventor： Vivek Srivastava ,  Markus Klinger ,  Rakhi Saikia ,  Vijaya Shankar Nataraj ,  Sohel Dalal ,  Jens Erik Nielsen

IPC: C11D3/386 ,  C11D11/00 ,  C12N9/24

Abstract: The present invention relates to mannanase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

公开(公告)号：US10920205B2

公开(公告)日：2021-02-16

申请号：US16439912

申请日：2019-06-13

Applicant: NOVOZYMES A/S

Inventor： Padmavathi Balumuri ,  Rakhi Saikia ,  Allan Svendsen ,  Lone Baunsgaard

IPC: C12N9/20 ,  C12N15/00 ,  C12P7/00 ,  C12P21/06 ,  C11D3/386 ,  C12N15/09 ,  C12P7/64

Abstract: The present invention relates to a lipase variant of a parent lipase, which variant has lipase activity, at least 75% but less than 100% sequence identity to SEQ ID NO: 3 and comprises a substitution at one or more positions corresponding to positions 1; 2; 3; 4; 5; 6; 7; 9; 10; 11; 12; 16; 19; 30; 31; 34; 36; 37; 39; 40; 42; 44; 51; 52; 53; 54; 56; 58; 59; 70; 71; 72; 73; 83; 84; 86; 88; 90; 92; 93; 95; 96; 100; 101; 102; 104; 106; 109; 110; 112; 117; 119; 124; 125; 127; 128; 131; 132; 133; 134; 135; 137; 158; 159; 160; 161; 162; 163; 165; 166; 167; 168; 170; 181; 182; 183; 189; 190; 192; 194; 196; 202; 210; 211; 212; 220; 225; 227; 228; 229; 230; 231; 233; 237; 238; 239; 240; 242; 246; 247; 248; 252; 259; 262; 264; 269 of SEQ ID NO: 3. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

公开(公告)号：US10683489B2

公开(公告)日：2020-06-16

申请号：US15310247

申请日：2015-05-26

Applicant: Novozymes A/S

Inventor： Allan Svendsen ,  Rakhi Saikia ,  Sohel Dalal ,  Vikram Kjoeller Bhatia ,  Lone Baunsgaard

IPC: C12N9/20

Abstract: The invention relates to a method for generating a preparation of lipase with increased lipolytic activity comprising a step of altering one or more nucleotides in a polynucleotide comprising a first polynucleotide encoding a propeptide operationally linked to a second polynucleotide encoding a lipase, wherein the alteration of one or more nucleotides are made in the first polynucleotide and the alteration independently results in a substitution, an insertion or a deletion in the encoded propeptide amino acid sequence.