中科微点-全球专利检索

  1. Login
  2. Sign Up

Search Results

23 records (took 0.025 seconds)

    Simultaneous Site-Specific Integrations of Multiple Gene-Copies
    11.
    发明申请
    Simultaneous Site-Specific Integrations of Multiple Gene-Copies 审中-公开

    公开(公告)号：US20170191081A1

    公开(公告)日：2017-07-06

    申请号：US15407757

    申请日：2017-01-17

    Applicant: Novozymes A/S

    Inventor： Hiroaki Udagawa

    IPC: C12N15/90 ,  C12N15/80

    CPC classification number: C12N15/902 ,  C12N15/80 ,  C12P21/02

    Abstract: The invention relates to a method for the simultaneous integration of two or more copies of a polynucleotide of interest into the chromosome of a fungal host cell comprising at least two pairs of recognition sequences of a site-specific recombinase, each pair flanking a resident negative selection marker; transformation of the cell with a construct carrying a gene of interest also flanked by the recognition sequences to ensure double-crossover events after transient expression of the recombinase, followed by selection for excision of all negative selection markers from the cell.

    Regulated PepC Expression
    12.
    发明申请
    Regulated PepC Expression 审中-公开
    调节PepC表达

    公开(公告)号：US20160244737A1

    公开(公告)日：2016-08-25

    申请号：US14913967

    申请日：2014-08-22

    Applicant: Novozymes A/S

    Inventor： Hiroshi Teramoto ,  Hiroaki Udagawa ,  Jan Lehmbeck ,  Michael Lynge Nielsen

    IPC: C12N9/62 ,  C12N9/18 ,  C12N15/80 ,  C12N9/20 ,  C12P21/02 ,  C12N9/82

    CPC classification number: C12N9/62 ,  C07K14/38 ,  C12N9/0036 ,  C12N9/18 ,  C12N9/20 ,  C12N9/58 ,  C12N9/82 ,  C12N15/80 ,  C12P21/02

    Abstract: The present invention relates to a recombinant fungal host cell comprising at least one first polynucleotide encoding a polypeptide of interest; and one or more second polynucleotide encoding a fungal PepC protease, wherein the one or more second polynucleotide is operably linked to a regulated heterologous promoter, as well as a method for producing a polypeptide of interest, comprising cultivating said fungal host cell.

    Abstract translation: 本发明涉及重组真菌宿主细胞，其包含至少一种编码目的多肽的第一多核苷酸; 和编码真菌PepC蛋白酶的一种或多种第二多核苷酸，其中所述一种或多种第二多核苷酸可操作地连接到受调节的异源启动子，以及用于产生感兴趣多肽的方法，包括培养所述真菌宿主细胞。

    Simultaneous Site-Specific Integrations of Multiple Gene-Copies in Filamentous Fungi
    14.
    发明申请
    Simultaneous Site-Specific Integrations of Multiple Gene-Copies in Filamentous Fungi 有权
    在丝状真菌中多个基因拷贝的同时位点特异性整合

    公开(公告)号：US20140120625A1

    公开(公告)日：2014-05-01

    申请号：US14118995

    申请日：2012-05-23

    Applicant: Novozymes A/S

    Inventor： Hiroaki Udagawa

    IPC: C12N15/80

    CPC classification number: C12N15/902 ,  C12N15/80 ,  C12P21/02

    Abstract: The invention relates to a method for the simultaneous integration of two or more copies of a polynucleotide of interest into the chromosome of a fungal host cell comprising at least two pairs of recognition sequences of a site-specific recombinase, each pair flanking a resident negative selection marker; transformation of the cell with a construct carrying a gene of interest also flanked by the recognition sequences to ensure double-crossover events after transient expression of the recombinase, followed by selection for excision of all negative selection markers from the cell.

    Abstract translation: 本发明涉及一种将目的多核苷酸的两个或多个拷贝同时整合到真菌宿主细胞的染色体中的方法，该方法包括至少两对位点特异性重组酶的识别序列，每一对位于宿主负选择 标记 带有携带感兴趣基因的构建体的细胞的转化也侧接识别序列，以确保重组酶瞬时表达后的双交换事件，随后从细胞中选择切除所有阴性选择标记。

    Modified Filamentous Fungal Host Cell
    15.
    发明申请
    Modified Filamentous Fungal Host Cell 有权

    公开(公告)号：US20210340511A1

    公开(公告)日：2021-11-04

    申请号：US17284163

    申请日：2019-10-08

    Applicant: Novozymes A/S

    Inventor： Hiroaki Udagawa

    IPC: C12N9/20 ,  C07K14/38 ,  C12N1/14 ,  C12N9/34

    Abstract: The present invention relates to filamentous fungal cells secreting a polypeptide of interest, wherein the expression of a citT gene is altered, reduced or eliminated compared to a non-mutated otherwise isogenic or parent cell, and methods of producing a secreted polypeptide of interest in said cells as well as methods of producing said cells.

    Polypeptides Having Protease Activity and Polynucleotides Encoding Same
    17.
    发明申请
    Polypeptides Having Protease Activity and Polynucleotides Encoding Same 审中-公开
    具有蛋白酶活性的多肽和编码相同的多核苷酸

    公开(公告)号：US20140295493A1

    公开(公告)日：2014-10-02

    申请号：US14242644

    申请日：2014-04-01

    Applicant: NOVOZYMES A/S

    Inventor： Hiroaki Udagawa ,  Christian Isak Jorgensen

    IPC: C12P21/00

    CPC classification number: C12P21/00 ,  C12N9/62 ,  C12N15/80

    Abstract: The present invention relates to isolated polypeptides having protease activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.

    Abstract translation: 本发明涉及具有蛋白酶活性的分离的多肽和编码多肽的分离的多核苷酸。 本发明还涉及包含多核苷酸的核酸构建体，载体和宿主细胞以及生产和使用多肽的方法。

    Methods For Eliminating Or Reducing The Expression Of A Gene In A Filamentous Fungal Strain
    18.
    发明申请
    Methods For Eliminating Or Reducing The Expression Of A Gene In A Filamentous Fungal Strain 审中-公开
    消除或减少丝状真菌菌株中基因表达的方法

    公开(公告)号：US20140287475A1

    公开(公告)日：2014-09-25

    申请号：US14255642

    申请日：2014-04-17

    Applicant: Novozymes A/S ,  Novozymes, Inc.

    Inventor： Howard Brody ,  Suchindra Maiyuran ,  Hiroaki Udagawa

    IPC: C12P1/02

    CPC classification number: C12P1/02 ,  C12N15/113 ,  C12N15/1137 ,  C12N2310/111 ,  C12N2310/14 ,  C12N2310/53

    Abstract: The present invention relates to methods for reducing or eliminating the expression of a target gene in a filamentous fungal strain, comprising: (a) inserting into the genome of the filamentous fungal strain a double-stranded transcribable nucleic acid construct comprising a first nucleotide sequence comprising a promoter operably linked to a homologous coding region of the target gene and a second nucleotide sequence comprising the homologous coding region, or a portion thereof, of the target gene, wherein the first and second nucleotide sequences are complementary to each other and the second nucleotide sequence is in reverse orientation relative to the first nucleotide sequence; and (b) inducing production of an interfering RNA encoded by the double-stranded transcribable nucleic acid construct by cultivating the filamentous fungal strain under conditions conducive for production of the interfering RNA; wherein the interfering RNA interacts with RNA transcripts of the target gene to reduce or eliminate expression of the target gene. The present invention also relates to the filamentous fungal strains and to methods of producing a biological substance of interest in such filamentous fungal strains.

    Abstract translation: 本发明涉及用于减少或消除丝状真菌菌株中靶基因表达的方法，其包括：（a）将丝状真菌菌株的双链可转录核酸构建体插入基因组中，所述双链可转录核酸构建体包含第一核苷酸序列，其包含 可操作地连接到靶基因的同源编码区的启动子和包含靶基因的同源编码区或其部分的第二核苷酸序列，其中第一和第二核苷酸序列彼此互补，第二核苷酸 序列相对于第一个核苷酸序列处于相反方向; 和（b）通过在有利于产生干扰RNA的条件下培养丝状真菌菌株诱导由双链可转录核酸构建体编码的干扰RNA的产生; 其中所述干扰RNA与所述靶基因的RNA转录物相互作用以减少或消除所述靶基因的表达。 本发明还涉及丝状真菌菌株以及在这种丝状真菌菌株中生产目的生物物质的方法。

    Simultaneous site-specific integrations of multiple gene-copies
    20.
    发明授权
    Simultaneous site-specific integrations of multiple gene-copies 有权

    公开(公告)号：US09850501B2

    公开(公告)日：2017-12-26

    申请号：US15407757

    申请日：2017-01-17

    Applicant: Novozymes A/S

    Inventor： Hiroaki Udagawa

    IPC: C12N15/80 ,  C12N15/90

    CPC classification number: C12N15/902 ,  C12N15/80 ,  C12P21/02

    Abstract: The invention relates to a method for the simultaneous integration of two or more copies of a polynucleotide of interest into the chromosome of a fungal host cell comprising at least two pairs of recognition sequences of a site-specific recombinase, each pair flanking a resident negative selection marker; transformation of the cell with a construct carrying a gene of interest also flanked by the recognition sequences to ensure double-crossover events after transient expression of the recombinase, followed by selection for excision of all negative selection markers from the cell.

Patent Agency Ranking