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    Beta-glucosidase variants polynucleotides encoding same
    12.
    发明授权
    Beta-glucosidase variants polynucleotides encoding same 有权

    公开(公告)号：US10260058B2

    公开(公告)日：2019-04-16

    申请号：US15617296

    申请日：2017-06-08

    Applicant: Novozymes, Inc.

    Inventor： Mark Wogulis ,  Paul Harris ,  David Osborn

    IPC: C12N9/42 ,  C12P19/14

    Abstract: The present invention relates to beta-glucosidase variants, e.g., beta-glucosidase variants of a parent Family GH3A beta-glucosidase from Aspergillus fumigatus. The present invention also relates to polynucleotides encoding the beta-glucosidase variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the beta-glucosidase variants.

    Variants of Glycoside Hydrolases
    14.
    发明申请
    Variants of Glycoside Hydrolases 审中-公开

    公开(公告)号：US20170298333A1

    公开(公告)日：2017-10-19

    申请号：US15629117

    申请日：2017-06-21

    Applicant: Novozymes Inc.

    Inventor： Sarah Teter ,  Connie Ward ,  Joel Cherry ,  Aubrey Jones ,  Paul Harris ,  Jung Yi

    IPC: C12N9/42 ,  C12P7/10 ,  C12P19/14

    CPC classification number: C12N9/2437 ,  C11D3/38645 ,  C12N9/2434 ,  C12N9/2445 ,  C12P7/10 ,  C12P19/02 ,  C12P19/14 ,  C12Y302/01021 ,  C12Y302/01091 ,  C12Y302/01176 ,  Y02E50/16

    Abstract: The present invention relates to variants of a parent glycoside hydrolase, comprising a substitution at one or more positions corresponding to positions 21, 94, 157, 205, 206, 247, 337, 350, 373, 383, 438, 455, 467, and 486 of amino acids 1 to 513 of SEQ ID NO: 2, and optionally further comprising a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2 a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2, wherein the variants have glycoside hydrolase activity. The present invention also relates to nucleotide sequences encoding the variant glycoside hydrolases and to nucleic acid constructs, vectors, and host cells comprising the nucleotide sequences.

    Methods for increasing homologous recombination of a nucleic acid sequence
    18.
    发明授权
    Methods for increasing homologous recombination of a nucleic acid sequence 失效

    公开(公告)号：US08481320B2

    公开(公告)日：2013-07-09

    申请号：US13710007

    申请日：2012-12-10

    Applicant: Novozymes, Inc.

    Inventor： Paul Harris ,  Howard Brody

    IPC: C12N15/00 ,  C12N1/15 ,  C12N15/64 ,  C12N15/84

    CPC classification number: C12P21/00 ,  C12N15/80 ,  C12N15/902

    Abstract: The present invention relates to methods for increasing homologous recombination of a nucleic acid sequence introduced into a host cell, comprising: (a) introducing into a population of filamentous fungal host cells a first nucleic acid sequence encoding a recombination protein and a second nucleic acid sequence comprising one or more regions which are homologous with the genome of the filamentous fungal host cell, wherein (i) the recombination protein promotes the recombination of the one or more regions with the corresponding homologous region in the host's genome to incorporate the second nucleic acid sequence by homologous recombination, and (ii) the number of host cells comprising the incorporated second nucleic acid sequence in the population is increased at least 20% compared to the same population without the first nucleic acid sequence; (b) and isolating from the population a filamentous fungal cell comprising the incorporated second nucleic acid sequence.

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