公开(公告)号：US20180237759A1

公开(公告)日：2018-08-23

申请号：US15955162

申请日：2018-04-17

Applicant: Novozymes, Inc.

Inventor： Sandra Merino ,  Keith McFarland ,  Joel Cherry ,  Sarah Teter

IPC: C12N9/42 ,  C12P7/10 ,  C12P19/14 ,  C07K14/37 ,  C12P19/16

CPC classification number: C12N9/2437 ,  C07K14/37 ,  C12N9/2445 ,  C12P7/10 ,  C12P19/14 ,  C12P19/16 ,  C12Y302/01004 ,  C12Y302/01021 ,  C12Y302/01091 ,  Y02E50/16 ,  Y02E50/17

Abstract: The present invention relates to cellulolytic compositions for degrading or converting cellulose-containing material and methods of producing and using the compositions.

公开(公告)号：US20170298333A1

公开(公告)日：2017-10-19

申请号：US15629117

申请日：2017-06-21

Applicant: Novozymes Inc.

Inventor： Sarah Teter ,  Connie Ward ,  Joel Cherry ,  Aubrey Jones ,  Paul Harris ,  Jung Yi

IPC: C12N9/42 ,  C12P7/10 ,  C12P19/14

CPC classification number: C12N9/2437 ,  C11D3/38645 ,  C12N9/2434 ,  C12N9/2445 ,  C12P7/10 ,  C12P19/02 ,  C12P19/14 ,  C12Y302/01021 ,  C12Y302/01091 ,  C12Y302/01176 ,  Y02E50/16

Abstract: The present invention relates to variants of a parent glycoside hydrolase, comprising a substitution at one or more positions corresponding to positions 21, 94, 157, 205, 206, 247, 337, 350, 373, 383, 438, 455, 467, and 486 of amino acids 1 to 513 of SEQ ID NO: 2, and optionally further comprising a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2 a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2, wherein the variants have glycoside hydrolase activity. The present invention also relates to nucleotide sequences encoding the variant glycoside hydrolases and to nucleic acid constructs, vectors, and host cells comprising the nucleotide sequences.

公开(公告)号：US20130130353A1

公开(公告)日：2013-05-23

申请号：US13748328

申请日：2013-01-23

Applicant: Novozymes, Inc.

Inventor： Sarah Teter ,  Connie Ward ,  Joel Cherry ,  Aubrey Jones ,  Paul Harris ,  Jung Yi

IPC: C12N9/24

CPC classification number: C12N9/2437 ,  C11D3/38645 ,  C12N9/2434 ,  C12N9/2445 ,  C12P7/10 ,  C12P19/02 ,  C12P19/14 ,  C12Y302/01021 ,  C12Y302/01091 ,  C12Y302/01176 ,  Y02E50/16

Abstract: The present invention relates to variants of a parent glycoside hydrolase, comprising a substitution at one or more positions corresponding to positions 21, 94, 157, 205, 206, 247, 337, 350, 373, 383, 438, 455, 467, and 486 of amino acids 1 to 513 of SEQ ID NO: 2, and optionally further comprising a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2 a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2, wherein the variants have glycoside hydrolase activity. The present invention also relates to nucleotide sequences encoding the variant glycoside hydrolases and to nucleic acid constructs, vectors, and host cells comprising the nucleotide sequences.

公开(公告)号：US10316344B2

公开(公告)日：2019-06-11

申请号：US15183474

申请日：2016-06-15

Applicant: Novozymes, Inc.

Inventor： Randy Berka ,  Joel Cherry

IPC: C12P19/14 ,  C12P7/26 ,  C12P7/00 ,  C12P7/02 ,  C12P7/04 ,  C12P19/02 ,  C12P17/04 ,  C12P13/04 ,  C12P7/40 ,  C12P7/28 ,  C12P5/02 ,  C12P7/20 ,  C12P7/18 ,  C12P7/16 ,  C12P7/10 ,  C12P7/06

Abstract: The present invention relates to methods for converting plant cell wall polysaccharides into one or more products, comprising: treating the plant cell wall polysaccharides with an effective amount of a spent whole fermentation broth of a recombinant microorganism, wherein the recombinant microorganism expresses one or more heterologous genes encoding enzymes which degrade or convert the plant cell wall polysaccharides into the one or more products. The present invention also relates to methods for producing an organic substance, comprising: (a) saccharifying plant cell wall polysaccharides with an effective amount of a spent whole fermentation broth of a recombinant microorganism, wherein the recombinant microorganism expresses one or more heterologous genes encoding enzymes which degrade or convert the plant cell wall polysaccharides into saccharified material; (b) fermenting the saccharified material of step (a) with one or more fermenting microoganisms; and (c) recovering the organic substance from the fermentation.

公开(公告)号：US20150056664A1

公开(公告)日：2015-02-26

申请号：US14475639

申请日：2014-09-03

Applicant: NOVOZYMES INC.

Inventor： Sarah Teter ,  Connie Ward ,  Joel Cherry ,  Aubrey Jones ,  Paul Harris ,  Jung Yi

IPC: C12N9/42 ,  C12P19/02 ,  C11D3/386 ,  C12P19/14

CPC classification number: C12N9/2437 ,  C11D3/38645 ,  C12N9/2434 ,  C12N9/2445 ,  C12P7/10 ,  C12P19/02 ,  C12P19/14 ,  C12Y302/01021 ,  C12Y302/01091 ,  C12Y302/01176

Abstract: The present invention relates to variants of a parent glycoside hydrolase, comprising a substitution at one or more positions corresponding to positions 21, 94, 157, 205, 206, 247, 337, 350, 373, 383, 438, 455, 467, and 486 of amino acids 1 to 513 of SEQ ID NO: 2, and optionally further comprising a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2 a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2, wherein the variants have glycoside hydrolase activity. The present invention also relates to nucleotide sequences encoding the variant glycoside hydrolases and to nucleic acid constructs, vectors, and host cells comprising the nucleotide sequences.

Abstract translation: 本发明涉及母体糖苷水解酶的变体，其包含在对应于位置21,94,157,205,206,247,337,350,373,383,438,455,467的一个或多个位置的取代，和 486，SEQ ID NO：2的氨基酸1至513，并且任选地还包含在对应于位置8,22,41,49,57,113,193,196,226,227,246,29的一个或多个位置的取代， SEQ ID NO：2的氨基酸1至513的251,255,259,301,356,371,411和462在对应于位置8,22,41,49,57,113， SEQ ID NO：2的氨基酸1至513的193,196,226,227,246,251,255,259,301,356,371,411和462，其中所述变体具有糖苷水解酶活性。 本发明还涉及编码变体糖苷水解酶的核苷酸序列以及包含核苷酸序列的核酸构建体，载体和宿主细胞。

公开(公告)号：US20140141472A1

公开(公告)日：2014-05-22

申请号：US14157383

申请日：2014-01-16

Applicant: NOVOZYMES, INC.

Inventor： Randy Berka ,  Joel Cherry

IPC: C12P19/14 ,  C12P19/02

CPC classification number: C12P19/14 ,  C12P5/023 ,  C12P7/00 ,  C12P7/02 ,  C12P7/04 ,  C12P7/06 ,  C12P7/10 ,  C12P7/16 ,  C12P7/18 ,  C12P7/20 ,  C12P7/26 ,  C12P7/28 ,  C12P7/40 ,  C12P13/04 ,  C12P17/04 ,  C12P19/02 ,  Y02E50/10 ,  Y02E50/16 ,  Y02E50/17 ,  Y02E50/343

Abstract: The present invention relates to methods for converting plant cell wall polysaccharides into one or more products, comprising: treating the plant cell wall polysaccharides with an effective amount of a spent whole fermentation broth of a recombinant microorganism, wherein the recombinant microorganism expresses one or more heterologous genes encoding enzymes which degrade or convert the plant cell wall polysaccharides into the one or more products. The present invention also relates to methods for producing an organic substance, comprising: (a) saccharifying plant cell wall polysaccharides with an effective amount of a spent whole fermentation broth of a recombinant microorganism, wherein the recombinant microorganism expresses one or more heterologous genes encoding enzymes which degrade or convert the plant cell wall polysaccharides into saccharified material; (b) fermenting the saccharified material of step (a) with one or more fermenting microoganisms; and (c) recovering the organic substance from the fermentation.

Abstract translation: 本发明涉及将植物细胞壁多糖转化为一种或多种产品的方法，其包括：用有效量的重组微生物的消耗的全发酵液处理植物细胞壁多糖，其中重组微生物表达一种或多种异源 编码将植物细胞壁多糖降解或转化成一种或多种产物的酶的基因。 本发明还涉及有机物质的制造方法，其特征在于，包括：（a）用有效量的重组微生物用的全部发酵肉汤糖化植物细胞壁多糖，其中所述重组微生物表达一种或多种编码酶的异源基因 其将植物细胞壁多糖降解或转化为糖化物质; （b）用一种或多种发酵微生物发酵步骤（a）的糖化物质; 和（c）从发酵中回收有机物质。

公开(公告)号：US09701951B2

公开(公告)日：2017-07-11

申请号：US14475639

申请日：2014-09-03

Applicant: Novozymes Inc.

Inventor： Sarah Teter ,  Connie Ward ,  Joel Cherry ,  Aubrey Jones ,  Paul Harris ,  Jung Yi

IPC: C12N9/42 ,  C12P19/14 ,  C11D3/386 ,  C12P19/02

CPC classification number: C12N9/2437 ,  C11D3/38645 ,  C12N9/2434 ,  C12N9/2445 ,  C12P7/10 ,  C12P19/02 ,  C12P19/14 ,  C12Y302/01021 ,  C12Y302/01091 ,  C12Y302/01176

Abstract: The present invention relates to variants of a parent glycoside hydrolase, comprising a substitution at one or more positions corresponding to positions 21, 94, 157, 205, 206, 247, 337, 350, 373, 383, 438, 455, 467, and 486 of amino acids 1 to 513 of SEQ ID NO: 2, and optionally further comprising a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2 a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2, wherein the variants have glycoside hydrolase activity. The present invention also relates to nucleotide sequences encoding the variant glycoside hydrolases and to nucleic acid constructs, vectors, and host cells comprising the nucleotide sequences.

公开(公告)号：US20160289722A1

公开(公告)日：2016-10-06

申请号：US15183474

申请日：2016-06-15

Applicant: Novozymes, Inc.

Inventor： Randy Berka ,  Joel Cherry

IPC: C12P19/14 ,  C12P19/02

CPC classification number: C12P19/14 ,  C12P5/023 ,  C12P7/00 ,  C12P7/02 ,  C12P7/04 ,  C12P7/06 ,  C12P7/10 ,  C12P7/16 ,  C12P7/18 ,  C12P7/20 ,  C12P7/26 ,  C12P7/28 ,  C12P7/40 ,  C12P13/04 ,  C12P17/04 ,  C12P19/02 ,  Y02E50/10 ,  Y02E50/16 ,  Y02E50/17 ,  Y02E50/343

Abstract: The present invention relates to methods for converting plant cell wall polysaccharides into one or more products, comprising: treating the plant cell wall polysaccharides with an effective amount of a spent whole fermentation broth of a recombinant microorganism, wherein the recombinant microorganism expresses one or more heterologous genes encoding enzymes which degrade or convert the plant cell wall polysaccharides into the one or more products. The present invention also relates to methods for producing an organic substance, comprising: (a) saccharifying plant cell wall polysaccharides with an effective amount of a spent whole fermentation broth of a recombinant microorganism, wherein the recombinant microorganism expresses one or more heterologous genes encoding enzymes which degrade or convert the plant cell wall polysaccharides into saccharified material; (b) fermenting the saccharified material of step (a) with one or more fermenting microoganisms; and (c) recovering the organic substance from the fermentation.

Abstract translation: 本发明涉及将植物细胞壁多糖转化为一种或多种产品的方法，其包括：用有效量的重组微生物的消耗的全发酵液处理植物细胞壁多糖，其中重组微生物表达一种或多种异源 编码将植物细胞壁多糖降解或转化成一种或多种产物的酶的基因。 本发明还涉及有机物质的制造方法，其特征在于，包括：（a）用有效量的重组微生物用的全部发酵肉汤糖化植物细胞壁多糖，其中所述重组微生物表达一种或多种编码酶的异源基因 其将植物细胞壁多糖降解或转化为糖化物质; （b）用一种或多种发酵微生物发酵步骤（a）的糖化物质; 和（c）从发酵中回收有机物质。