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    Novel Carbonyl Reductase, Gene Therefor and Use Thereof
    11.
    发明申请
    Novel Carbonyl Reductase, Gene Therefor and Use Thereof 失效
    新型羰基还原酶，其基因及其用途

    公开(公告)号：US20090029430A1

    公开(公告)日：2009-01-29

    申请号：US11665065

    申请日：2005-10-20

    申请人： Noriyuki Kizaki ,  Mokoto Ueda ,  Daisuke Moriyama ,  Naoaki Taoka ,  Yoshihiko Yasohara

    发明人： Noriyuki Kizaki ,  Mokoto Ueda ,  Daisuke Moriyama ,  Naoaki Taoka ,  Yoshihiko Yasohara

    IPC分类号： C12P7/02 ,  C07H21/04 ,  C07K14/00 ,  C12N15/63 ,  C12N1/21

    CPC分类号： C12N9/0006 ,  C12P7/04 ,  C12P7/62 ,  C12P13/002 ,  C12P41/002

    摘要： The present invention is to provide a process for efficiently producing an optically active alcohol including (R)-3-hydroxy-3-phenylpropanenitrile. One of the features of the present invention is a polypeptide having an activity of asymmetrically reducing 3-oxo-3-phenylpropanenitrile isolated from a microorganism belonging to the genus Candida to produce (R)-3-hydroxy-3-phenylpropanenitrile, DNA encoding the polypeptide and a transformant of producing the polypeptide. Another feature of the present invention is a process for producing an optically active alcohol such as (R)-3-hydroxy-3-phenylpropanenitrile by reducing a carbonyl compound such as 3-oxo-3-phenylpropanenitrile by use of the polypeptide or the transformant.

    摘要翻译： 本发明提供一种有效制备包含（R）-3-羟基-3-苯基丙腈的光学活性醇的方法。 本发明的特征之一是具有不对称还原从属于念珠菌属的微生物分离的3-氧代-3-苯基丙腈的活性的多肽，以产生（R）-3-羟基-3-苯基丙腈，编码 多肽和产生多肽的转化体。 本发明的另一个特征是通过使用多肽或转化体还原羰基化合物如3-氧代-3-苯基丙腈来制备光学活性醇如（R）-3-羟基-3-苯基丙腈的方法 。

    Method for producing L-amino acid
    12.
    发明授权
    Method for producing L-amino acid 有权
    生产L-氨基酸的方法

    公开(公告)号：US09464306B2

    公开(公告)日：2016-10-11

    申请号：US13126534

    申请日：2009-10-28

    申请人： Masutoshi Nojiri ,  Tozo Nishiyama ,  Naoaki Taoka

    发明人： Masutoshi Nojiri ,  Tozo Nishiyama ,  Naoaki Taoka

    IPC分类号： C12P13/04 ,  C12P13/00 ,  C12N9/10 ,  C12N9/90 ,  C07C227/30 ,  C07C233/47 ,  C12P13/22 ,  C12P41/00

    CPC分类号： C12P13/04 ,  C07C227/30 ,  C07C233/47 ,  C12N9/104 ,  C12N9/90 ,  C12P13/222 ,  C12P41/007 ,  Y02P20/52 ,  C07C229/30 ,  C07C229/32 ,  C07C229/36

    摘要： The present invention relates to a method for producing an L-amino acid by reacting an enantiomeric mixture of an N-succinyl amino acid with L-succinylase in the presence of N-acylamino acid racemase to specifically hydrolyze the L-form. In particular, the present invention relates to a method for producing an L-amino acid in high yield by using an N-succinyl amino acid whose dissolved concentration is particularly low as a raw material to perform a reaction while precipitating the produced L-amino acid out of the reaction system. The present invention enables efficient production of an L-amino acid having high optical purity, particularly an L-amino acid useful as a raw material for products such as pharmaceutical products and agricultural chemicals.

    摘要翻译： 本发明涉及通过在N-酰基氨基酸消旋酶存在下使N-琥珀酰氨基酸与L-琥珀酰基酶的对映异构体混合物反应以特异性水解L型而生产L-氨基酸的方法。 特别地，本发明涉及通过使用溶解浓度特别低的N-琥珀酰氨基酸作为原料进行反应，同时使生成的L-氨基酸沉淀而以高产率生产L-氨基酸的方法 出了反应体系。 本发明能够有效地生产具有高光学纯度的L-氨基酸，特别是可用作药物产品和农药等产品的原料的L-氨基酸。

    Novel (S,S) -Butanediol Dehydrogenase, Gene for the Same, and Use of the Same
    13.
    发明申请
    Novel (S,S) -Butanediol Dehydrogenase, Gene for the Same, and Use of the Same 审中-公开
    新型（S，S） - 丁二醇脱氢酶，其基因及其用途

    公开(公告)号：US20110143406A1

    公开(公告)日：2011-06-16

    申请号：US12223319

    申请日：2007-02-02

    申请人： Daisuke Moriyama ,  Naoaki Taoka

    发明人： Daisuke Moriyama ,  Naoaki Taoka

    IPC分类号： C12P7/26 ,  C07H21/04 ,  C12N9/04 ,  C12N15/63 ,  C12N1/21 ,  C12P7/18

    CPC分类号： C12Y101/01304 ,  C12N9/0006 ,  C12P7/18 ,  C12P41/002

    摘要： The present invention has its object to provide a novel (S,S)-butanediol dehydrogenase. The present invention also has its object to provide a gene coding for the enzyme protein, a vector containing the gene, a transformant harboring the vector, and a method of producing an optically active alcohol using the transformant. The polypeptide according to one embodiment of the present invention has the physicochemical properties including actions such as: acting on (2S,3S)-2,3-butanediol to form (S)-acetoin in the presence of NAD+ as a coenzyme; and reducing 2,3-butanedione to form (2S,3S)-2,3-butanediol in the presence of NADH as a coenzyme.

    摘要翻译： 本发明的目的是提供一种新型（S，S） - 丁二醇脱氢酶。 本发明的目的还在于提供编码酶蛋白的基因，含有该基因的载体，携带该载体的转化体，以及使用该转化体制造光学活性醇的方法。 根据本发明一个实施方案的多肽具有包括以下作用的物理化学性质：如在NAD +作为辅酶存在下作用于（2S，3S）-2,3-丁二醇以形成（S） - 乙酰胸苷; 并在NADH作为辅酶存在下还原2,3-丁二酮以形成（2S，3S）-2,3-丁二醇。

    Process for Producing Optically Active Secondary Alcohol
    14.
    发明申请
    Process for Producing Optically Active Secondary Alcohol 审中-公开
    生产光活性二级醇的方法

    公开(公告)号：US20080038803A1

    公开(公告)日：2008-02-14

    申请号：US11885056

    申请日：2006-02-24

    申请人： Akira Iwasaki ,  Motohisa Washida ,  Naoaki Taoka ,  Daisuke Moriyama ,  Junzou Hasegawa

    发明人： Akira Iwasaki ,  Motohisa Washida ,  Naoaki Taoka ,  Daisuke Moriyama ,  Junzou Hasegawa

    IPC分类号： C12P7/02 ,  C12N1/00

    CPC分类号： C12P41/002 ,  C12N9/0008 ,  C12N9/0036 ,  C12P7/02 ,  C12P7/18

    摘要： The present invention provides a method enabling convenient production of an optically active secondary alcohol useful as a pharmaceutical intermediate, particularly an optically active 1,2-diol and an optically active 2-alkanol, from an enantiomer mixture thereof. An oxidizing enzyme source having the capability of selectively oxidizing one enantiomer of secondary alcohol is allowed to act on an enantiomer mixture of secondary alcohol in the presence of a reducing enzyme source having the capability of reverse enantio-selectively reducing a ketone derivative, to convert the enantiomer mixture into a substantially single enantiomer at a theoretical percent recovery of 100%, whereby an optically active secondary alcohol is produced.

    摘要翻译： 本发明提供了一种能够方便地制备可用作药物中间体，特别是光学活性的1,2-二醇和光学活性2-链烷醇的光学活性仲醇的方法。 在具有反向对映选择性还原酮衍生物的能力的还原酶源的存在下，使具有选择性氧化仲醇的对映异构体的氧化酶源作用于仲醇的对映体混合物， 对映异构体混合物变成基本上单一的对映体，理论回收率为100％，由此产生光学活性的仲醇。

    Process for producing optically active 3-hydroxypropionic ester derivative
    15.
    发明申请
    Process for producing optically active 3-hydroxypropionic ester derivative 有权
    光学活性3-羟基丙酸酯衍生物的制备方法

    公开(公告)号：US20060166342A1

    公开(公告)日：2006-07-27

    申请号：US10536768

    申请日：2003-12-08

    申请人： Naoaki Taoka ,  Daisuke Moriyama ,  Kohei Mori ,  Takahiro Oishi

    发明人： Naoaki Taoka ,  Daisuke Moriyama ,  Kohei Mori ,  Takahiro Oishi

    IPC分类号： C12P7/62

    CPC分类号： C12P7/62 ,  C07C67/313 ,  C07D317/60 ,  C12P17/04 ,  C12P41/002 ,  C07C69/716

    摘要： The present invention is to provide a process for simply producing an optically active 3-hydroxypropionic ester derivative useful as a medicament intermediate from an inexpensive material. More specifically, the present invention is directed to a process for producing an optically active 3-hydroxypropionic ester derivative comprising reacting an acetic ester derivative available at low cost with abase and a formic ester, thereby converting the acetic ester derivative into a 2-formylacetic ester derivative, and thereafter, stereospecifically reducing the formyl group of the derivative by use of an enzymatic source capable of stereoselectively reducing the formyl group of the derivative.

    摘要翻译： 本发明提供一种简单地制造用作廉价材料的药物中间体的光学活性3-羟基丙酸酯衍生物的方法。 更具体地说，本发明涉及一种光学活性3-羟基丙酸酯衍生物的制备方法，其包括将低成本获得的乙酸酯衍生物与甲酸酯反应，由此将乙酸酯衍生物转化为2-甲酰基乙酸酯 衍生物，然后通过使用能够立体选择性还原衍生物的甲酰基的酶源立体选择性还原衍生物的甲酰基。

    Chlorohydroxyacetone derivative and process for producing optically active chloropropanediol derivative from the same
    16.
    发明授权
    Chlorohydroxyacetone derivative and process for producing optically active chloropropanediol derivative from the same 失效
    氯羟丙酮衍生物及其制备光学活性氯丙二醇衍生物的方法

    公开(公告)号：US06682916B2

    公开(公告)日：2004-01-27

    申请号：US10069105

    申请日：2002-02-26

    申请人： Naoaki Taoka ,  Hironobu Maeda ,  Kazumi Okuro ,  Koichiro Toyota ,  Yoshihiko Yasohara

    发明人： Naoaki Taoka ,  Hironobu Maeda ,  Kazumi Okuro ,  Koichiro Toyota ,  Yoshihiko Yasohara

    IPC分类号： F12P718

    CPC分类号： C12P41/002 ,  C07B2200/07 ,  C07C45/29 ,  C07C49/255 ,  C12P7/04 ,  C12P7/18 ,  C12P7/26 ,  C07C49/175

    摘要： A process for efficiently preparing an optically active chloropropanediol derivative, especially (S)-1-benzyloxy-3-chloro-2-propanol, which has a high optical purity and is useful as an intermediate for medicines. The process comprises treating an inexpensive racemic chloropropanediol derivative with a nitroxyl compound and an oxidizing agent to convert it into a chlorohydroxyacetone derivative and then stereospecifically reducing the carbonyl group of the chlorohydroxyacetone derivative by the action of either a carbonyl-reducing enzyme having the ability to stereospecifically reduce the chlorohydroxyacetone derivative or an optically treated culture of a microorganism having the ability to yield the carbonyl-reducing enzyme. Thus, an optically active chloropropanediol derivative is prepared.

    摘要翻译： 一种有效制备光学活性氯丙醇二醇衍生物，特别是（S）-1-苄氧基-3-氯-2-丙醇的方法，该方法具有高的光学纯度并且可用作药物的中间体。 该方法包括用硝酰化合物和氧化剂处理廉价的外消旋氯丙二醇衍生物，将其转化成氯羟丙酮衍生物，然后通过具有立体特异性的羰基还原酶的作用立即特异性还原氯羟基丙酮衍生物的羰基 减少氯代羟基丙酮衍生物或具有产生羰基还原酶能力的微生物的光学处理培养物。 因此，制备光学活性氯丙二醇衍生物。

    Carbonyl reductase, gene therefor and use thereof
    19.
    发明授权
    Carbonyl reductase, gene therefor and use thereof 失效
    羰基还原酶，其基因及其用途

    公开(公告)号：US08008461B2

    公开(公告)日：2011-08-30

    申请号：US11665065

    申请日：2005-10-20

    申请人： Noriyuki Kizaki ,  Makoto Ueda ,  Daisuke Moriyama ,  Naoaki Taoka ,  Yoshihiko Yasohara

    发明人： Noriyuki Kizaki ,  Makoto Ueda ,  Daisuke Moriyama ,  Naoaki Taoka ,  Yoshihiko Yasohara

    IPC分类号： C07H21/04 ,  C12N9/02 ,  C12P21/02 ,  C12P13/00 ,  C07C255/01

    CPC分类号： C12N9/0006 ,  C12P7/04 ,  C12P7/62 ,  C12P13/002 ,  C12P41/002

    摘要： The present invention is to provide a process for efficiently producing an optically active alcohol including (R)-3-hydroxy-3-phenylpropanenitrile. One of the features of the present invention is a polypeptide having an activity of asymmetrically reducing 3-oxo-3-phenylpropanenitrile isolated from a microorganism belonging to the genus Candida to produce (R)-3-hydroxy-3-phenylpropanenitrile, DNA encoding the polypeptide and a transformant of producing the polypeptide. Another feature of the present invention is a process for producing an optically active alcohol such as (R)-3-hydroxy-3-phenylpropanenitrile by reducing a carbonyl compound such as 3-oxo-3-phenylpropanenitrile by use of the polypeptide or the transformant.

    摘要翻译： 本发明提供一种有效制备包含（R）-3-羟基-3-苯基丙腈的光学活性醇的方法。 本发明的特征之一是具有不对称还原从属于念珠菌属的微生物分离的3-氧代-3-苯基丙腈的活性的多肽，以产生（R）-3-羟基-3-苯基丙腈，编码 多肽和产生多肽的转化体。 本发明的另一个特征是通过使用多肽或转化体还原羰基化合物如3-氧代-3-苯基丙腈来制备光学活性醇如（R）-3-羟基-3-苯基丙腈的方法 。

    Process for production of optically active-3-amino-2-hydroxypropionic cyclopropylamide derivatives and salts thereof
    20.
    发明授权
    Process for production of optically active-3-amino-2-hydroxypropionic cyclopropylamide derivatives and salts thereof 有权
    光学活性-3-氨基-2-羟基丙基丙烯酰胺衍生物及其盐的制备方法

    公开(公告)号：US07834190B2

    公开(公告)日：2010-11-16

    申请号：US12227788

    申请日：2007-05-22

    申请人： Kohei Mori ,  Akira Nishiyama ,  Naoaki Taoka ,  Daisuke Moriyama ,  Nobuo Nagashima

    发明人： Kohei Mori ,  Akira Nishiyama ,  Naoaki Taoka ,  Daisuke Moriyama ,  Nobuo Nagashima

    IPC分类号： C07D263/08 ,  C07D303/02

    CPC分类号： C07B53/00 ,  C07B2200/07 ,  C07C67/31 ,  C07C231/18 ,  C07C237/04 ,  C07C237/22 ,  C07C2601/02 ,  C07D263/16 ,  C07D301/24 ,  C07D303/16 ,  C07D303/48 ,  C12P7/42 ,  C12P13/02 ,  C12P41/002 ,  C07C69/675

    摘要： An objective of the present application is to provide an industrially practicable method for producing an optically-active 3-amino-2-hydroxypropionic cyclopropylamide derivative or salt thereof from an inexpensive easily-available starting material. The derivative or salt thereof is useful as an intermediate for a medicine. It is also intended by the present application to provide a useful intermediate of the derivative. The objective is attained by the following method. First, an easily-available 2-halo-3-oxopropionic acid derivative is asymmetrically reduced, and then epoxidated to produce an optically-active epoxycarboxylic acid derivative. Next, the derivative is converted into an optically-active epoxyamide derivative by reaction with cyclopropylamine, and then reacted with a nitrile to obtain an optically-active oxazolinamide derivative. Subsequently, selective acid solvolysis of the oxazoline skeleton gives the optically-active 3-amino-2-hydroxypropionic cyclopropylamide derivative or salt thereof.

    摘要翻译： 本申请的目的是提供从便宜的廉价起始材料制备光学活性3-氨基-2-羟基丙基环丙基酰胺衍生物或其盐的工业上可行的方法。 其衍生物或其盐可用作药物的中间体。 本申请还希望提供衍生物的有用中间体。 目的是通过以下方法实现。 首先，容易获得的2-卤代-3-氧代丙酸衍生物被不对称地还原，然后环氧化以产生光学活性的环氧羧酸衍生物。 接下来，通过与环丙胺反应将衍生物转化为光学活性的环氧酰胺衍生物，然后与腈反应，得到光学活性的恶唑啉酰胺衍生物。 随后，恶唑啉骨架的选择性酸溶剂解产生光学活性3-氨基-2-羟基丙基丙烯酰胺衍生物或其盐。