公开(公告)号：US20160168547A1

公开(公告)日：2016-06-16

申请号：US15051169

申请日：2016-02-23

Applicant: Novozymes Inc. ,  Novozymes A/S

Inventor： Suzanne Otani ,  Debbie Yaver ,  Haiyan Ge ,  Janine Lin ,  Christopher Amolo ,  Kim Borch ,  Shamkant Anant Patkar ,  Michael Lamsa ,  Barbara Cherry

IPC: C12N9/20

CPC classification number: C12N9/20 ,  C12Y301/01003

Abstract: The present invention relates to isolated polypeptides having lipase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.

Abstract translation: 本发明涉及具有脂肪酶活性的分离的多肽和编码多肽的分离的多核苷酸。 本发明还涉及包含多核苷酸的核酸构建体，载体和宿主细胞以及生产和使用多肽的方法。

公开(公告)号：US09273104B2

公开(公告)日：2016-03-01

申请号：US14081340

申请日：2013-11-15

Applicant: Novozymes A/S ,  Novozymes, Inc.

Inventor： Mads Bjornvad ,  Debbie Yaver

IPC: C07K7/00 ,  C12N9/20 ,  C07K14/37 ,  C12N15/62 ,  C12N15/81 ,  C12P21/02 ,  A61K38/00

CPC classification number: C12P21/02 ,  C07K14/37 ,  C07K2319/02 ,  C12N9/20 ,  C12N15/625 ,  C12N15/81 ,  C12Y301/01003

Abstract: The present invention relates to methods for producing a polypeptide having biological activity, comprising: (a) cultivating a fungal host cell in a medium conducive for the production of the polypeptide, wherein the fungal host cell comprises a first polynucleotide encoding the polypeptide operably linked to a second polynucleotide encoding a variant signal peptide or a variant prepropeptide; and (b) isolating the secreted polypeptide having biological activity from the cultivation medium.

公开(公告)号：US20140142278A1

公开(公告)日：2014-05-22

申请号：US14081340

申请日：2013-11-15

Applicant: Novozymes, Inc. ,  Novozymes A/S

Inventor： Mads Bjornvad ,  Debbie Yaver

IPC: C07K14/37

CPC classification number: C12P21/02 ,  C07K14/37 ,  C07K2319/02 ,  C12N9/20 ,  C12N15/625 ,  C12N15/81 ,  C12Y301/01003

Abstract: The present invention relates to methods for producing a polypeptide having biological activity, comprising: (a) cultivating a fungal host cell in a medium conducive for the production of the polypeptide, wherein the fungal host cell comprises a first polynucleotide encoding the polypeptide operably linked to a second polynucleotide encoding a variant signal peptide or a variant prepropeptide; and (b) isolating the secreted polypeptide having biological activity from the cultivation medium.

Abstract translation: 本发明涉及生产具有生物活性的多肽的方法，其包括：（a）在有助于产生多肽的培养基中培养真菌宿主细胞，其中真菌宿主细胞包含编码多肽的第一多核苷酸，其可操作地连接到 编码变体信号肽或变体前肽的第二个多核苷酸; 和（b）从培养基中分离具有生物活性的分泌多肽。

公开(公告)号：US08563286B2

公开(公告)日：2013-10-22

申请号：US13762135

申请日：2013-02-07

Applicant: Novozymes, Inc. ,  Novozymes A/S

Inventor： Debbie Yaver ,  Janine Lin ,  Michael Lamsa ,  Kim Borch ,  Shamkant Anant Patkar

IPC: C12N9/10 ,  C12N15/00 ,  C12N1/20 ,  C07H21/04

CPC classification number: C12N9/20

Abstract: The present invention relates to isolated polypeptides having lipase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.

公开(公告)号：US20130152234A1

公开(公告)日：2013-06-13

申请号：US13762135

申请日：2013-02-07

Applicant: Novozymes, Inc. ,  Novozymes A/S

Inventor： Debbie Yaver ,  Janine Lin ,  Michael Lamsa ,  Kim Borch ,  Shamkant Anant Patkar

IPC: C12N9/20

CPC classification number: C12N9/20

Abstract: The present invention relates to isolated polypeptides having lipase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.

公开(公告)号：US20130123163A1

公开(公告)日：2013-05-16

申请号：US13747184

申请日：2013-01-22

Applicant: Novozymes, Inc. ,  Novozymes A/S

Inventor： Suzanne Otani ,  Debbie Yaver ,  Haiyan Ge ,  Janine Lin ,  Christopher Amolo ,  Kim Borch ,  Shamkant Anant Patkar ,  Michael Lamsa ,  Barbara Cherry

IPC: C12N9/20

CPC classification number: C12N9/20 ,  C12Y301/01003

Abstract: The present invention relates to isolated polypeptides having lipase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.

Abstract translation: 本发明涉及具有脂肪酶活性的分离的多肽和编码多肽的分离的多核苷酸。 本发明还涉及包含多核苷酸的核酸构建体，载体和宿主细胞以及生产和使用多肽的方法。