公开(公告)号：US20100090127A1

公开(公告)日：2010-04-15

申请号：US12517653

申请日：2008-01-23

申请人： Ahmad Yekta ,  Pavel A. Fomitchov ,  Joseph Masino

发明人： Ahmad Yekta ,  Pavel A. Fomitchov ,  Joseph Masino

IPC分类号： G01J1/58

CPC分类号： G01N21/6458 ,  G01N21/6408 ,  G01N2021/6439 ,  G01N2021/6441

摘要： This invention provides a system and method that allows for time-resolved fluorescent imaging of fluorescent samples. The user is able to receive temporally filtered pictures of the sample with a reduced amount of the scattered excitation light and the short lived background fluorescence. The system allows for adjustment of fluorescent gating time and delay time.

摘要翻译： 本发明提供允许荧光样品的时间分辨荧光成像的系统和方法。 用户能够以减少量的散射激发光和短寿命背景荧光来接收样本的时间滤波图像。 该系统允许调节荧光门控时间和延迟时间。

公开(公告)号：US07567346B2

公开(公告)日：2009-07-28

申请号：US11363938

申请日：2006-03-01

申请人： Pavel A. Fomitchov ,  Eugene Barash ,  Ahmad Yetka ,  Joseph Masino, III

发明人： Pavel A. Fomitchov ,  Eugene Barash ,  Ahmad Yetka ,  Joseph Masino, III

IPC分类号： G01N21/25

CPC分类号： G01N21/6458 ,  G01N21/47 ,  G01N2201/0407 ,  G01N2201/1042 ,  G02B21/0048 ,  G02B21/0076 ,  G02B21/367

摘要： A system and method for multimode imaging of at least one sample is disclosed. The system includes at least one light source; an optical system selected responsive to a mode of operation of the imaging system; and a detector capable of selective reading of pixels. The at least one sample is moved elative to the optical system using a sample movement technique selected from the group consisting of step sample moving and continuous sample moving. The method includes the steps of (1) selecting a mode of operation for the imaging system; (2) transmitting light from at least one light source through an optical system selected in response to the mode of operation for the imaging system; (3) moving the at least one sample relative to the optical system using a sample movement technique selected from the group consisting of step sample moving and continuous sample moving; and (4) selectively reading pixels with a detector.

摘要翻译： 公开了一种用于至少一个样品的多模成像的系统和方法。 该系统包括至少一个光源; 响应于成像系统的操作模式选择的光学系统; 以及能够选择性地读取像素的检测器。 使用选自步骤样品移动和连续样品移动的样品移动技术将至少一个样品移动到光学系统。 该方法包括以下步骤：（1）选择成像系统的操作模式; （2）从至少一个光源透射通过响应于成像系统的操作模式选择的光学系统的光; （3）使用选自步骤样品移动和连续样品移动的样本移动技术来移动所述至少一个样品相对于所述光学系统; 和（4）用检测器选择性地读取像素。

公开(公告)号：US07473877B2

公开(公告)日：2009-01-06

申请号：US11930206

申请日：2007-10-31

申请人： Pavel A. Fomitchov

发明人： Pavel A. Fomitchov

IPC分类号： G02B7/04

CPC分类号： G02B21/245 ,  G02B7/36 ,  G02B21/006 ,  G02B21/244

摘要： The invention relates to a method for adjusting focus in an automated microscope. The method may comprise the steps of: providing an optical detector for image acquisition, wherein the optical detector comprises an array of sensor pixels; designating a region of interest in the array of sensor pixels to emulate a confocal aperture; directing a light beam to illuminate an object according to a predefined pattern, thereby forming an image of the illuminated pattern at the optical detector, wherein the image of the illuminated pattern substantially overlaps the designated region of interest; detecting a light intensity from sensor pixels located within the designated region of interest; and adjusting a relative focal position of an objective lens based on the detected light intensity.

摘要翻译： 本发明涉及一种用于在自动显微镜中调节焦点的方法。 该方法可以包括以下步骤：提供用于图像获取的光学检测器，其中所述光学检测器包括传感器像素阵列; 指定传感器像素阵列中的感兴趣区域以模拟共焦孔; 引导光束根据预定图案照射物体，从而在光学检测器处形成照明图案的图像，其中照射图案的图像基本上与指定的感兴趣区域重叠; 从位于所述指定的感兴趣区域内的传感器像素检测光强度; 以及基于检测到的光强度来调整物镜的相对焦点位置。

公开(公告)号：US08063386B2

公开(公告)日：2011-11-22

申请号：US12517653

申请日：2008-01-23

申请人： Ahmad Yekta ,  Pavel A. Fomitchov ,  Joseph Masino

发明人： Ahmad Yekta ,  Pavel A. Fomitchov ,  Joseph Masino

IPC分类号： G01N21/64

CPC分类号： G01N21/6458 ,  G01N21/6408 ,  G01N2021/6439 ,  G01N2021/6441

摘要： A system and method that allows for time-resolved fluorescent imaging of fluorescent samples. The user is able to receive temporally filtered pictures of the sample with a reduced amount of the scattered excitation light and the short lived background fluorescence. The system allows for adjustment of fluorescent gating time and delay time.

摘要翻译： 允许荧光样品的时间分辨荧光成像的系统和方法。 用户能够以减少量的散射激发光和短寿命背景荧光来接收样本的时间滤波图像。 该系统允许调节荧光门控时间和延迟时间。

公开(公告)号：US07953308B2

公开(公告)日：2011-05-31

申请号：US11320675

申请日：2005-12-30

申请人： Pavel A. Fomitchov

发明人： Pavel A. Fomitchov

IPC分类号： G02B6/06

CPC分类号： G02B6/06

摘要： A system and method for fiber optic bundle-based illumination for an imaging system is disclosed. According to one embodiment of the present invention, the system includes a fiber optic bundle that comprising a plurality of optical fibers. Each optical fiber receives light from a light source. The system also includes a beam forming element that selects a mode of operation for the imaging system. The system further includes a beam deflecting device that deflects the light on a sample. According to one embodiment of the present invention, the method includes the steps of (1) selecting a mode of operation for the imaging system; (2) transmitting light from at least one light source through a fiber optic bundle, the fiber optic bundle comprising a plurality of optical fibers; (3) providing a beam forming element based on the selected mode of operation; and (4) deflecting the focused light on a sample.

摘要翻译： 公开了一种用于成像系统的基于光纤束的照明的系统和方法。 根据本发明的一个实施例，该系统包括包括多个光纤的光纤束。 每个光纤接收来自光源的光。 该系统还包括选择成像系统的操作模式的波束形成元件。 该系统还包括使光在样品上偏转的光束偏转装置。 根据本发明的一个实施例，该方法包括以下步骤：（1）选择成像系统的操作模式; （2）从至少一个光源透过光纤束，所述光纤束包括多根光纤; （3）基于所选择的操作模式提供波束形成元件; 和（4）将聚焦光偏转到样品上。

公开(公告)号：USD603965S1

公开(公告)日：2009-11-10

申请号：US29277414

申请日：2007-02-26

申请人： Kaushal Verma ,  Joseph Masino ,  Pavel A. Fomitchov ,  Liqin Wang

设计人： Kaushal Verma ,  Joseph Masino ,  Pavel A. Fomitchov ,  Liqin Wang

公开(公告)号：US07297910B2

公开(公告)日：2007-11-20

申请号：US11320676

申请日：2005-12-30

申请人： Pavel A. Fomitchov

发明人： Pavel A. Fomitchov

IPC分类号： G02B7/04 ,  G02B27/40 ,  G03B13/00

CPC分类号： G02B21/245 ,  G02B7/36 ,  G02B21/006 ,  G02B21/244

摘要： The invention relates to a method for adjusting focus in an automated microscope. The method may comprise the steps of: providing an optical detector for image acquisition, wherein the optical detector comprises an array of sensor pixels; designating a region of interest in the array of sensor pixels to emulate a confocal aperture; directing a light beam to illuminate an object according to a predefined pattern, thereby forming an image of the illuminated pattern at the optical detector, wherein the image of the illuminated pattern substantially overlaps the designated region of interest; detecting a light intensity from sensor pixels located within the designated region of interest; and adjusting a relative focal position of an objective lens based on the detected light intensity.

摘要翻译： 本发明涉及一种用于在自动显微镜中调节焦点的方法。 该方法可以包括以下步骤：提供用于图像获取的光学检测器，其中所述光学检测器包括传感器像素阵列; 指定传感器像素阵列中的感兴趣区域以模拟共焦孔; 引导光束根据预定图案照射物体，从而在光学检测器处形成照明图案的图像，其中照射图案的图像基本上与指定的感兴趣区域重叠; 从位于所述指定的感兴趣区域内的传感器像素检测光强度; 以及基于检测到的光强度来调整物镜的相对焦点位置。