公开(公告)号：US07567346B2

公开(公告)日：2009-07-28

申请号：US11363938

申请日：2006-03-01

申请人： Pavel A. Fomitchov ,  Eugene Barash ,  Ahmad Yetka ,  Joseph Masino, III

发明人： Pavel A. Fomitchov ,  Eugene Barash ,  Ahmad Yetka ,  Joseph Masino, III

IPC分类号： G01N21/25

CPC分类号： G01N21/6458 ,  G01N21/47 ,  G01N2201/0407 ,  G01N2201/1042 ,  G02B21/0048 ,  G02B21/0076 ,  G02B21/367

摘要： A system and method for multimode imaging of at least one sample is disclosed. The system includes at least one light source; an optical system selected responsive to a mode of operation of the imaging system; and a detector capable of selective reading of pixels. The at least one sample is moved elative to the optical system using a sample movement technique selected from the group consisting of step sample moving and continuous sample moving. The method includes the steps of (1) selecting a mode of operation for the imaging system; (2) transmitting light from at least one light source through an optical system selected in response to the mode of operation for the imaging system; (3) moving the at least one sample relative to the optical system using a sample movement technique selected from the group consisting of step sample moving and continuous sample moving; and (4) selectively reading pixels with a detector.

摘要翻译： 公开了一种用于至少一个样品的多模成像的系统和方法。 该系统包括至少一个光源; 响应于成像系统的操作模式选择的光学系统; 以及能够选择性地读取像素的检测器。 使用选自步骤样品移动和连续样品移动的样品移动技术将至少一个样品移动到光学系统。 该方法包括以下步骤：（1）选择成像系统的操作模式; （2）从至少一个光源透射通过响应于成像系统的操作模式选择的光学系统的光; （3）使用选自步骤样品移动和连续样品移动的样本移动技术来移动所述至少一个样品相对于所述光学系统; 和（4）用检测器选择性地读取像素。

公开(公告)号：US20130093873A1

公开(公告)日：2013-04-18

申请号：US13704748

申请日：2011-06-29

申请人： Witold Bula ,  Pavel A. Fomitchov ,  Steve Goodson ,  Erwen Mei ,  Yang Zhang

发明人： Witold Bula ,  Pavel A. Fomitchov ,  Steve Goodson ,  Erwen Mei ,  Yang Zhang

IPC分类号： H04N5/04

CPC分类号： H04N5/04 ,  G02B21/0048 ,  H04N1/113 ,  H04N1/195 ,  H04N2201/04715 ,  H04N2201/04755

摘要： A line confocal microscope system, comprising a scanning unit in the form of a mechanically driven scanning unit with a controllable a scanning trajectory and a two dimensional sensor unit operated in a rolling line shutter mode in synchronization with the scanning unit, wherein the scanning trajectory is set to have an acceleration part outside the field of view of the sensor unit.

摘要翻译： 一种线共聚焦显微镜系统，包括具有可控扫描轨迹的机械驱动扫描单元形式的扫描单元和与扫描单元同步地以滚动线快门模式操作的二维传感器单元，其中扫描轨迹为 设置为在传感器单元的视场外具有加速部分。

公开(公告)号：US10027855B2

公开(公告)日：2018-07-17

申请号：US13704748

申请日：2011-06-29

申请人： Witold Bula ,  Pavel A. Fomitchov ,  Steve Goodson ,  Erwen Mei ,  Yang Zhang

发明人： Witold Bula ,  Pavel A. Fomitchov ,  Steve Goodson ,  Erwen Mei ,  Yang Zhang

IPC分类号： H04N5/04 ,  G02B21/00 ,  H04N1/113 ,  H04N1/195

CPC分类号： H04N5/04 ,  G02B21/0048 ,  H04N1/113 ,  H04N1/195 ,  H04N2201/04715 ,  H04N2201/04755

摘要： A line confocal microscope system, comprising a scanning unit in the form of a mechanically driven scanning unit with a controllable a scanning trajectory and a two dimensional sensor unit operated in a rolling line shutter mode in synchronization with the scanning unit, wherein the scanning trajectory is set to have an acceleration part outside the field of view of the sensor unit.

公开(公告)号：US20140140595A1

公开(公告)日：2014-05-22

申请号：US14129640

申请日：2012-06-27

申请人： Pavel A. Fomitchov ,  Witold Bula

发明人： Pavel A. Fomitchov ,  Witold Bula

IPC分类号： G02B21/36 ,  G06T7/00

CPC分类号： G02B21/365 ,  G01N21/6458 ,  G02B21/0076 ,  G02B21/008 ,  G02B21/16 ,  G06T7/0012

摘要： Microscopy system for biological imaging, comprising an image quality monitoring system for monitoring image quality of an image of a biological sample comprising a biological object selection means arranged to let a user of the system to select one or more Biological Reference Objects (BRO) in the image of the biological sample, and an image quality evaluation means arranged to compare the signal level of image pixels of the one or more BROs with an image background signal level to calculate one or more image quality parameters for the image of the biological sample. The system is arranged to present the image quality parameters to the user as an indication of the image quality specific for the BRO(s).

摘要翻译： 用于生物成像的显微镜系统，包括用于监测生物样品的图像的图像质量的图像质量监测系统，所述图像质量监测系统包括生物物体选择装置，所述生物物体选择装置被布置为使得所述系统的用户选择所述生物样本中的一个或多个生物参考对象 图像质量评估装置，其被布置为将一个或多个BRO的图像像素的信号电平与图像背景信号电平进行比较，以计算生物样本的图像的一个或多个图像质量参数。 系统被布置为向用户呈现图像质量参数作为对BRO特有的图像质量的指示。

公开(公告)号：US08053711B2

公开(公告)日：2011-11-08

申请号：US12523066

申请日：2008-01-28

申请人： Pavel A. Fomitchov ,  Kaushal Verma ,  Joseph Masino

发明人： Pavel A. Fomitchov ,  Kaushal Verma ,  Joseph Masino

IPC分类号： G02B7/04

CPC分类号： G02B27/0068

摘要： A spherical aberration adjustment system is disclosed, which includes a plurality of objective lenses, where at least one of the plurality of objective lenses has a spherical aberration collar. The plurality of objective lenses are mounted onto an objective holder, where the objective holder is configured to place the at least one of the plurality of objective lenses in an imaging position. A driving mechanism is coupled by a mechanical link to the at least one of the plurality of objective lenses, where the mechanical link is configured to transmit motion from the driving mechanism to the spherical aberration collar. A control system is configured to manipulate the driving mechanism to move the spherical aberration collar of the at least one of the plurality of objective lenses in the imaging position to a specific spherical aberration adjustment setting.

摘要翻译： 公开了一种球面像差调整系统，其包括多个物镜，其中多个物镜中的至少一个具有球面像差轴环。 多个物镜安装在物镜支架上，其中物镜支架构造成将多个物镜中的至少一个放置在成像位置。 驱动机构通过机械连杆耦合到多个物镜中的至少一个物镜，其中机械连杆构造成将运动从驱动机构传递到球面像差轴环。 控制系统被配置为操纵驱动机构以将成像位置中的多个物镜中的至少一个的球面像差轴环移动到特定的球面像差调整设置。

公开(公告)号：US20100033812A1

公开(公告)日：2010-02-11

申请号：US12523066

申请日：2008-01-28

申请人： Pavel A. Fomitchov ,  Kaushal Verma ,  Joseph Masino

发明人： Pavel A. Fomitchov ,  Kaushal Verma ,  Joseph Masino

IPC分类号： G02B7/09 ,  G02B7/14 ,  G02B21/02

CPC分类号： G02B27/0068

摘要： A spherical aberration adjustment system is disclosed, which includes a plurality of objective lenses, where at least one of the plurality of objective lenses has a spherical aberration collar. The plurality of objective lenses are mounted onto an objective holder, where the objective holder is configured to place the at least one of the plurality of objective lenses in an imaging position. A driving mechanism is coupled by a mechanical link to the at least one of the plurality of objective lenses, where the mechanical link is configured to transmit motion from the driving mechanism to the spherical aberration collar. A control system is configured to manipulate the driving mechanism to move the spherical aberration collar of the at least one of the plurality of objective lenses in the imaging position to a specific spherical aberration adjustment setting.

摘要翻译： 公开了一种球面像差调整系统，其包括多个物镜，其中多个物镜中的至少一个具有球面像差轴环。 多个物镜安装在物镜支架上，其中物镜支架构造成将多个物镜中的至少一个放置在成像位置。 驱动机构通过机械连杆耦合到多个物镜中的至少一个物镜，其中机械连杆构造成将运动从驱动机构传递到球面像差轴环。 控制系统被配置为操纵驱动机构以将成像位置中的多个物镜中的至少一个的球面像差轴环移动到特定的球面像差调整设置。

公开(公告)号：US09373158B2

公开(公告)日：2016-06-21

申请号：US13813197

申请日：2011-07-19

申请人： Pavel A. Fomitchov ,  Witold Bula ,  Carlos Zarate ,  Yang Zhang

发明人： Pavel A. Fomitchov ,  Witold Bula ,  Carlos Zarate ,  Yang Zhang

IPC分类号： G06K9/00 ,  G06T5/00 ,  H04N1/409 ,  H04N5/357

CPC分类号： G06T5/00 ,  G06T5/005 ,  H04N1/409 ,  H04N5/357

摘要： The present invention relates to a system and method for reducing image artifacts for a CMOS camera used as an optical detector for a line confocal fluorescent imager. The method for reducing image artifacts in a monochromatic image comprises subtracting RGB channel offset mismatch; and correcting random row offset for each row. Preferably, the method also comprises correcting random column offset and gain. Optionally, the method also comprises clipping of defective pixels, and/or subtracting dark current. When the monochromatic image is a fluorescence image, the method also comprises a step of compressing CMOS camera noise. Also provided are a system for reducing image artifacts in a monochromatic image from a CMOS camera, as well as an image artifact reduction system for a CMOS camera-based line confocal fluorescent microscope.

摘要翻译： 本发明涉及一种用于减少用作线共焦荧光成像仪的光学检测器的CMOS相机的图像伪影的系统和方法。 用于减少单色图像中的图像伪像的方法包括减去RGB信道偏移不匹配; 并校正每行的随机行偏移量。 优选地，该方法还包括校正随机列偏移和增益。 可选地，该方法还包括限制缺陷像素和/或减去暗电流。 当单色图像是荧光图像时，该方法还包括压缩CMOS相机噪声的步骤。 还提供了一种用于减少来自CMOS照相机的单色图像中的图像伪影的系统，以及用于基于CMOS照相机的线共聚焦荧光显微镜的图像伪像减少系统。

公开(公告)号：US20110261179A1

公开(公告)日：2011-10-27

申请号：US12765204

申请日：2010-04-22

申请人： Pavel A. Fomitchov

发明人： Pavel A. Fomitchov

IPC分类号： H04N7/18

CPC分类号： H04N7/18 ,  A61B90/30 ,  A61B2090/304 ,  A61B2090/3616 ,  A61B2090/3618

摘要： The invention relates to an imaging system for use in operating rooms and other applications. According to one example, the imaging system may include a visible light source configured to illuminate a surgical field with visible light and an excitation source configured to generate excitation light to excite a fluorescent substance in an organism within the surgical field. The imaging system may also include an imaging lens system including a first sensor configured to receive visible light reflected from the surgical field via a first spectral channel and a second sensor configured to receive a fluorescent emission emitted from the fluorescent substance in the organism via a second spectral channel. The imaging system may further include a plurality of fixed magnification lenses configured to optically couple image signals to the first sensor and the second sensor, and a control unit configured to receive and digitally process the image signals to generate a plurality of image frames in order to rescale the plurality of image frames for display to a surgeon.

摘要翻译： 本发明涉及一种用于手术室和其他应用的成像系统。 根据一个示例，成像系统可以包括被配置为用可见光照射外科视野的可见光源和被配置为产生激发光以激发外科视野内的生物体中的荧光物质的激发源。 成像系统还可以包括成像透镜系统，该成像透镜系统包括被配置为经由第一光谱通道接收从外科视野反射的可见光的第一传感器，以及经配置以经由第二传感器接收从生物体中的荧光物质发射的荧光发射的第二传感器 频谱通道。 成像系统还可以包括多个固定放大透镜，其被配置为将图像信号光耦合到第一传感器和第二传感器，以及控制单元，被配置为接收和数字处理图像信号以产生多个图像帧，以便 重新调整多个图像帧以便显示给外科医生。

公开(公告)号：US20100090127A1

公开(公告)日：2010-04-15

申请号：US12517653

申请日：2008-01-23

申请人： Ahmad Yekta ,  Pavel A. Fomitchov ,  Joseph Masino

发明人： Ahmad Yekta ,  Pavel A. Fomitchov ,  Joseph Masino

IPC分类号： G01J1/58

CPC分类号： G01N21/6458 ,  G01N21/6408 ,  G01N2021/6439 ,  G01N2021/6441

摘要： This invention provides a system and method that allows for time-resolved fluorescent imaging of fluorescent samples. The user is able to receive temporally filtered pictures of the sample with a reduced amount of the scattered excitation light and the short lived background fluorescence. The system allows for adjustment of fluorescent gating time and delay time.

摘要翻译： 本发明提供允许荧光样品的时间分辨荧光成像的系统和方法。 用户能够以减少量的散射激发光和短寿命背景荧光来接收样本的时间滤波图像。 该系统允许调节荧光门控时间和延迟时间。

公开(公告)号：US07473877B2

公开(公告)日：2009-01-06

申请号：US11930206

申请日：2007-10-31

申请人： Pavel A. Fomitchov

发明人： Pavel A. Fomitchov

IPC分类号： G02B7/04

CPC分类号： G02B21/245 ,  G02B7/36 ,  G02B21/006 ,  G02B21/244

摘要： The invention relates to a method for adjusting focus in an automated microscope. The method may comprise the steps of: providing an optical detector for image acquisition, wherein the optical detector comprises an array of sensor pixels; designating a region of interest in the array of sensor pixels to emulate a confocal aperture; directing a light beam to illuminate an object according to a predefined pattern, thereby forming an image of the illuminated pattern at the optical detector, wherein the image of the illuminated pattern substantially overlaps the designated region of interest; detecting a light intensity from sensor pixels located within the designated region of interest; and adjusting a relative focal position of an objective lens based on the detected light intensity.

摘要翻译： 本发明涉及一种用于在自动显微镜中调节焦点的方法。 该方法可以包括以下步骤：提供用于图像获取的光学检测器，其中所述光学检测器包括传感器像素阵列; 指定传感器像素阵列中的感兴趣区域以模拟共焦孔; 引导光束根据预定图案照射物体，从而在光学检测器处形成照明图案的图像，其中照射图案的图像基本上与指定的感兴趣区域重叠; 从位于所述指定的感兴趣区域内的传感器像素检测光强度; 以及基于检测到的光强度来调整物镜的相对焦点位置。