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公开(公告)号:US09598473B2
公开(公告)日:2017-03-21
申请号:US14124629
申请日:2012-06-08
IPC分类号: C07K14/415 , A01H5/12 , A01H5/00 , C12N15/82 , A01H1/02
CPC分类号: C12N15/8243 , A01H1/02 , A01H1/04 , A01H5/00 , A01H5/12 , C07K14/415 , C12N15/8286 , C12P5/007 , Y02A40/162
摘要: The present invention relates to identification and isolation of zinc finger transcription factor in tomato that specifically expresses in glandular trichomes of Solanum lycopersicum cultivar Moneymaker and binds to the promoters of the genes encoding Terpene Synthase 5 (also known as Monoterpene Synthase 1) and Terpene Synthase 11 (also known as Sesquiterpene Synthase 1). The invention provides the isolated, recombinant or synthetic polynucleotides encoding the polypeptide sequences of SEQ ID NO:2 and variants and fragments thereof. The invention also provides constructs, vectors, host cells and plants genetically modified to contain the polynucleotides of the invention. The methods for producing plants with altered levels of terpenes, including transformed and mutant plants, are also provided.
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公开(公告)号:US20140173771A1
公开(公告)日:2014-06-19
申请号:US14124629
申请日:2012-06-08
IPC分类号: C07K14/415 , A01H5/12 , A01H5/00 , C12N15/82 , A01H1/02
CPC分类号: C12N15/8243 , A01H1/02 , A01H1/04 , A01H5/00 , A01H5/12 , C07K14/415 , C12N15/8286 , C12P5/007 , Y02A40/162
摘要: The present invention relates to identification and isolation of zinc finger transcription factor in tomato that specifically expresses in glandular trichomes of Solanum lycopersicum cultivar Moneymaker and binds to the promoters of the genes encoding Terpene Synthase 5 (also known as Monoterpene Synthase 1) and Terpene Synthase 11 (also known as Sesquiterpene Synthase 1). The invention provides the isolated, recombinant or synthetic polynucleotides encoding the polypeptide sequences of SEQ ID NO:2 and variants and fragments thereof. The invention also provides constructs, vectors, host cells and plants genetically modified to contain the polynucleotides of the invention. The methods for producing plants with altered levels of terpenes, including transformed and mutant plants, are also provided.
摘要翻译: 本发明涉及番茄中锌指转录因子的鉴定和分离,其特异性表达在番茄茄子栽培品种Moneymaker的腺体毛中,并结合编码萜烯合酶5(也称为单萜合酶1)和萜烯合酶11的基因的启动子 (也称为倍半萜合酶1)。 本发明提供编码SEQ ID NO:2的多肽序列的分离的,重组的或合成的多核苷酸及其变体和片段。 本发明还提供构建体,载体,宿主细胞和遗传修饰以包含本发明的多核苷酸的植物。 还提供了用于生产具有改变的萜烯含量的植物的方法,包括转化和突变植物。
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公开(公告)号:US20080127368A1
公开(公告)日:2008-05-29
申请号:US11661758
申请日:2005-09-01
申请人: Michel Albertus Haring , Robert Cornelis Schuurink , Julian Cornelis Verdonk , Arjen J. VanTunen
发明人: Michel Albertus Haring , Robert Cornelis Schuurink , Julian Cornelis Verdonk , Arjen J. VanTunen
IPC分类号: C12N15/82 , C12P21/04 , C07K16/18 , C07K14/00 , C12N15/11 , C12N15/00 , C12Q1/68 , C12N5/06 , C12N5/04 , C12N1/20 , C12N1/00 , A01H5/00
CPC分类号: C12N15/1135 , C07K14/415 , C12N15/8243 , C12N15/8251 , C12N15/8254 , C12N2310/111 , C12N2310/14
摘要: The present invention relates to a polypeptide which belongs to the R3R3-type MYB family and which regulates the shikimate pathway towards the production of benzenoids. The shikimate pathway is a biosynthesis pathway through which the three essential aromatic amino acids tyrosine, phenylalanine and tryptophan are synthesized in plants, bacteria and fungi. The present invention provides for the first time a regulatory protein in the shikimate pathway and a means to regulate the biosynthesis of these three essential amino acids which cannot be produced by mammals. At the same time, it opens up the way for the regulation of the biosynthesis of aromatic and non-aromatic compounds which are derived from these essential amino acids. A polypeptide or polynucleotide of the invention may be used in a method for manipulating the transcript levels of the genes of the shikimate pathway towards benzenoids for instance the genes of 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase (DAHPS), 5-enol-pyruvylshikimate-3-phosphate synthase (EPSPS), L-phenylalanine ammonia-lyase (PAL) and chorismate mutase (CM).
摘要翻译: 本发明涉及一种属于R3R3型MYB家族的多肽,其调节脐橙通路生产苯并芘。 莽草酸通路是一种生物合成途径,三种必需芳香族氨基酸酪氨酸,苯丙氨酸和色氨酸在植物,细菌和真菌中合成。 本发明首次提供了莽草酸通路中的调节蛋白和调节哺乳动物不能产生的这三种必需氨基酸的生物合成的方法。 同时,它开辟了调控由这些必需氨基酸衍生的芳族和非芳族化合物的生物合成的方式。 本发明的多肽或多核苷酸可用于操作萜品酸通路基因转录成水平的方法,例如3-脱氧-D-阿拉伯 - 庚酮酸7-磷酸合酶(DAHPS)的基因, 5-烯醇 - 丙酮酸莽草酸-3-磷酸合成酶(EPSPS),L-苯丙氨酸氨裂解酶(PAL)和草酸盐变位酶(CM)。
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