公开(公告)号：US20220106578A1

公开(公告)日：2022-04-07

申请号：US17555557

申请日：2021-12-20

Applicant: Protalix Ltd.

Inventor： Lilach CHEN ZELTSBURG ,  Ilya RUDERFER ,  Avidor SHULMAN ,  Liat FUX ,  Yulia UGORTSEV ,  Hagit NETA ,  Sivan GELLEY ,  Elad LAVEE LAVIAD ,  Yoseph SHAALTIEL

IPC: C12N9/22 ,  C12N9/16 ,  A61K38/46 ,  A61P43/00 ,  A61P11/00

Abstract: Modified DNase I protein in which one or more amino acids of a DNase I protein are modified non-cellularly, are provided. The modified DNase I protein exhibits a DNA hydrolytic activity in the presence of actin and an improved DNA hydrolytic activity compared to a homologous non-modified DNase I protein. Processes of preparing the modified DNase I protein and uses thereof in, for example, reducing a DNA content in sputum and/or in treating a disease or condition associated with excess extracellular DNA in a fluid, secretion or tissue of a subject, are also provided.

公开(公告)号：US20180112201A1

公开(公告)日：2018-04-26

申请号：US15540264

申请日：2016-01-04

Applicant: Protalix Ltd.

Inventor： Lilach CHEN ZELTSBURG ,  Ilya RUDERFER ,  Avidor SHULMAN ,  Liat FUX ,  Yulia UGORTSEV ,  Hagit NETA ,  Sivan GELLEY ,  Elad LAVEE LAVIAD ,  Yoseph SHAALTIEL

IPC: C12N9/22 ,  A61P11/00 ,  A61P43/00

CPC classification number: C12N9/22 ,  A61K38/00 ,  A61P11/00 ,  A61P43/00 ,  C12N9/16

Abstract: Modified DNase I protein in which one or more amino acids of a DNase I protein are modified non-cellularly, are provided. The modified DNase I protein exhibits a DNA hydrolytic activity in the presence of actin and an improved DNA hydrolytic activity compared to a homologous non-modified DNase I protein. Processes of preparing the modified DNase I protein and uses thereof in, for example, reducing a DNA content in sputum and/or in treating a disease or condition associated with excess extracellular DNA in a fluid, secretion or tissue of a subject, are also provided.

公开(公告)号：US20160053247A1

公开(公告)日：2016-02-25

申请号：US14936720

申请日：2015-11-10

Applicant: Protalix Ltd.

Inventor： Avidor SHULMAN ,  Ilya RUDERFER ,  Tehila BEN-MOSHE ,  Talia SHEKHTER ,  Yaniv AZULAY ,  Yoseph SHAALTIEL ,  Tali KIZHNER

IPC: C12N9/40

CPC classification number: C12N9/2465 ,  A61K38/00 ,  C12Y302/01022

Abstract: Multimeric protein structures comprising at least two alpha-galactosidase monomers being covalently linked to one another via a linking moiety are disclosed herein, as well a process for preparing same, and methods of treating Fabry disease via administration of a multimeric protein structure. The disclosed multimeric protein structures exhibit an improved performance, in terms of enhanced activity and/or a longer lasting activity under both lysosomal conditions and in a serum environment.

Abstract translation: 本文公开了包含通过连接部分彼此共价连接的至少两个α-半乳糖苷酶单体的多聚体蛋白质结构，以及其制备方法，以及通过施用多聚体蛋白质结构治疗法布里病的方法。 根据在溶酶体条件和血清环境中增强的活性和/或更持久的活性方面，所公开的多聚体蛋白质结构表现出改进的性能。

公开(公告)号：US20160017021A1

公开(公告)日：2016-01-21

申请号：US14773348

申请日：2014-03-06

Applicant: PROTALIX LTD.

Inventor： Yoseph SHAALTIEL ,  Uri HANANIA ,  Tali KIZHNER ,  Tami ARIEL ,  Svetlana GINGIS-VELITSKI

IPC: C07K14/715 ,  A61K38/17 ,  C07K14/415

Abstract: A plant produced TNF alpha polypeptide inhibitor is provided. Also provided are methods of generating and using same.

Abstract translation: 提供了植物产生的TNFα多肽抑制剂。 还提供了产生和使用它们的方法。

公开(公告)号：US20160017020A1

公开(公告)日：2016-01-21

申请号：US14773360

申请日：2014-03-06

Applicant: PROTALIX LTD.

Inventor： Yoseph SHAALTIEL ,  Uri HANANIA ,  Tali KIZHNER ,  Tami ARIEL ,  Svetlana GINGIS-VELITSKI

IPC: C07K14/705 ,  C07K14/415 ,  A61K36/81 ,  C12N15/82

CPC classification number: C07K14/70578 ,  A61K36/81 ,  A61K38/1793 ,  C07K14/415 ,  C07K14/525 ,  C07K14/7151 ,  C07K2319/02 ,  C07K2319/04 ,  C07K2319/30 ,  C12N5/04 ,  C12N5/10 ,  C12N5/14 ,  C12N15/09 ,  C12N15/62 ,  C12N15/82 ,  C12N15/8257 ,  C12N15/8258 ,  Y02A50/406

Abstract: A plant produced chimeric polypeptide is provided. The plant produced chimeric polypeptide comprising: (i) a first domain which comprises a TNF Alpha binding domain of a TNF receptor, and (ii) a second domain which comprises an Fc domain of an immunoglobulin, wherein the first domain and the second domain are N-terminally to C-terminally respectively sequentially translationally fused and wherein the chimeric polypeptide specifically binds TNF Alpha.

公开(公告)号：US20130177538A1

公开(公告)日：2013-07-11

申请号：US13727632

申请日：2012-12-27

Applicant: Protalix Ltd.

Inventor： Yoseph SHAALTIEL ,  Gideon Baum ,  Daniel Bartfeld ,  Sharon Hashmueli ,  Ayala Lewkowicz

IPC: A61K36/23

CPC classification number: A61K38/47 ,  A61K9/0053 ,  A61K36/23 ,  C07K2319/04 ,  C12N9/2405 ,  C12N9/2434 ,  C12N9/2465 ,  C12N15/8257 ,  C12P21/005 ,  C12Y302/01022 ,  C12Y302/01045

Abstract: A device, system and method for producing glycosylated proteins in plant culture, particularly proteins having a high mannose glycosylation, while targeting such proteins with an ER signal and/or by-passing the Golgi. The invention further relates to vectors and methods for expression and production of enzymatically active high mannose lysosomal enzymes using transgenic plant root, particularly carrot cells. More particularly, the invention relates to host cells, particularly transgenic suspended carrot cells, vectors and methods for high yield expression and production of biologically active high mannose Glucocerebrosidase (GCD). The invention further provides for compositions and methods for the treatment of lysosomal storage diseases.

Abstract translation: 用于在植物培养物中生产糖基化蛋白质的装置，系统和方法，特别是具有高甘露糖糖基化的蛋白质，同时用ER信号靶向这种蛋白质和/或旁路高尔基体。 本发明进一步涉及使用转基因植物根特别是胡萝卜细胞表达和产生酶活性高甘露糖溶酶体酶的载体和方法。 更具体地，本发明涉及宿主细胞，特别是转基因悬浮的胡萝卜细胞，用于高产量表达和生产生物活性高甘露糖葡糖脑苷脂酶（GCD）的载体和方法。 本发明还提供了用于治疗溶酶体贮积病的组合物和方法。