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    Tannase, gene encoding same, and process for producing same
    11.
    发明授权
    Tannase, gene encoding same, and process for producing same 有权
    鞣酸酶，编码基因的基因及其制备方法

    公开(公告)号：US08617865B2

    公开(公告)日：2013-12-31

    申请号：US13124510

    申请日：2009-10-02

    申请人： Megumi Nakagawa ,  Naoki Matsumoto ,  Hitoshi Amano ,  Shotaro Yamaguchi

    发明人： Megumi Nakagawa ,  Naoki Matsumoto ,  Hitoshi Amano ,  Shotaro Yamaguchi

    IPC分类号： C12N9/14

    CPC分类号： C12N9/18 ,  C12P7/42

    摘要： Disclosed is a thermostable tannase derived from a microorganism. Specifically disclosed is a thermostable tannase derived from Aspergillus awamori or Aspergillus niger. A preferred embodiment of the tannase has the following chemoenzymatic properties: (1) activity: to act on a depside bond to thereby cause hydrolysis; (2) molecular weight: about 230,000 Da (as measured by gel filtration); and (3) thermal stability: stable at a temperature up to 65° C. (pH 5.0, 30 min.)

    摘要翻译： 公开了一种衍生自微生物的热稳定性鞣酸酶。 具体公开了源自泡盛曲霉或黑曲霉的热稳定性鞣酸酶。 鞣酸酶的优选实施方案具有以下化学酶学性质：（1）活性：作用于脱附键从而引起水解; （2）分子量：约230,000Da（通过凝胶过滤测量）; 和（3）热稳定性：在高达65℃的温度下稳定（pH5.0,30分钟）

    METHOD FOR DESIGNING MUTATED ENZYME, METHOD FOR PREPARING THE SAME, AND MUTATED ENZYME
    13.
    发明申请
    METHOD FOR DESIGNING MUTATED ENZYME, METHOD FOR PREPARING THE SAME, AND MUTATED ENZYME 审中-公开
    用于设计突变酶的方法，其制备方法和突变酶

    公开(公告)号：US20090280553A1

    公开(公告)日：2009-11-12

    申请号：US12309929

    申请日：2007-06-28

    申请人： Bunzo Mikami ,  Hiroyuki Iwamoto ,  Shotaro Yamaguchi

    发明人： Bunzo Mikami ,  Hiroyuki Iwamoto ,  Shotaro Yamaguchi

    IPC分类号： C12N9/24 ,  C12N9/44 ,  C12N9/26 ,  C07H21/04 ,  C12N1/21

    CPC分类号： C12Y302/01041 ,  C07K2299/00 ,  C12N9/2451 ,  C12N9/2457 ,  C12N9/246 ,  C12Y302/01068

    摘要： It is intended to provide a novel method for improving an enzyme hydrolyzing an a-1,6-glycosidic linkage. A mutated enzyme is designed by specifying one or more amino acids selected from the group shown below in an amino acid sequence of an enzyme (an enzyme to be mutated) that hydrolyzes an a-1,6-glycosidic linkage, that is, the group consisting of an amino acid corresponding to an amino acid at the 292 position, an amino acid corresponding to an amino acid at the 371 position, an amino acid corresponding to an amino acid at the 406 position, an amino acid corresponding to an amino acid at the 407 position, an amino acid corresponding to an amino acid at the 437 position, an amino acid corresponding to an amino acid at the 465 position, an amino acid corresponding to an amino acid at the 475 position, an amino acid corresponding to an amino acid at the 476 position; an amino acid corresponding to an amino acid at the 525 position, an amino acid corresponding to an amino acid at the 526 position, an amino acid corresponding to an amino acid at the 580 position and an amino acid corresponding to an amino acid at the 582 position of the amino acid represented by in SEQ ID NO: 2 (step (1)) and constructing an amino acid sequence in which the amino acid(s) specified in the step (1) is/are substituted with another amino acid or deleted based on the amino acid sequence of the enzyme to be mutated (step (2)).

    摘要翻译： 旨在提供一种改善水解a-1,6-糖苷键的酶的新方法。 突变酶通过在水解a-1,6-糖苷键的酶（待突变的酶）的氨基酸序列中指定一个或多个选自下文所示的氨基酸来设计，即，该基团 由对应于292位氨基酸的氨基酸，对应于371位氨基酸的氨基酸，对应于406位氨基酸的氨基酸，对应于氨基酸的氨基酸 407位，对应于437位氨基酸的氨基酸，对应于465位氨基酸的氨基酸，对应于475位氨基酸的氨基酸，对应于氨基酸的氨基酸 酸在476位; 对应于525位氨基酸的氨基酸，对应于526位氨基酸的氨基酸，对应于580位氨基酸的氨基酸和对应于582位氨基酸的氨基酸 并且构建其中在步骤（1）中规定的氨基酸被其它氨基酸所取代的氨基酸序列的氨基酸序列（步骤（1））所示的氨基酸的位置） 基于要突变的酶的氨基酸序列（步骤（2））。

    Novel protein-deamidating enzyme, microorganism producing the same, gene encoding the same, production process therefor, and use thereof
    14.
    发明申请
    Novel protein-deamidating enzyme, microorganism producing the same, gene encoding the same, production process therefor, and use thereof 有权

    公开(公告)号：US20090081763A1

    公开(公告)日：2009-03-26

    申请号：US12289226

    申请日：2008-10-23

    申请人： Shotaro Yamaguchi

    发明人： Shotaro Yamaguchi

    IPC分类号： C12N1/20

    CPC分类号： C12N9/80 ,  A23J3/34

    摘要： A method for the production of an enzyme, which comprises culturing in a medium a strain that belongs to a bacterium classified into Cytophagales or Actinomycetes, or a new bacterium Chryseobacterium sp. No. 9670 belonging to the genus Chryseobacterium, and has the ability to produce an enzyme having a property to deamidate amido groups in protein, thereby effecting production of the enzyme, and subsequently collecting the enzyme from the culture mixture and a method for the modification of protein making use of a novel enzyme which directly acts upon amido groups in protein, as well as a gene which encodes the enzyme, a recombinant vector which contains the gene, a transformant transformed with the vector and a method in which the transformant is cultured in a medium to effect production of the protein-deamidating enzyme and then the protein-deamidating enzyme is collected from the culture mixture. It is possible to provide a novel protein-deamidating enzyme which has an activity to release side chain carboxyl groups and ammonia from a protein by acting upon side chain amido groups in the protein, a microorganism capable of producing the same, a gene encoding the same, a production process therefor and use thereof.

    Protein-deamidating enzyme, microorganism producing the same, gene encoding the same, production process therefor, and use thereof
    16.
    发明授权
    Protein-deamidating enzyme, microorganism producing the same, gene encoding the same, production process therefor, and use thereof 有权
    蛋白质脱酰胺酶，产生其的微生物，编码基因的生产方法及其用途

    公开(公告)号：US06756221B1

    公开(公告)日：2004-06-29

    申请号：US09727769

    申请日：2000-12-04

    申请人： Shotaro Yamaguchi

    发明人： Shotaro Yamaguchi

    IPC分类号： C12N978

    CPC分类号： C12N9/80 ,  A23J3/34

    摘要： A method for the production of an enzyme, which comprises culturing in a medium a strain that belongs to a bacterium classified into Cytophagales or Actinomycetes, or a new bacterium Chryseobacterium sp. No. 9670 belonging to the genus Chryseobacterium, and has the ability to produce an enzyme having a property to deamidate amido groups in protein, thereby effecting production of the enzyme, and subsequently collecting the enzyme from the culture mixture and a method for the modification of protein making use of a novel enzyme which directly acts upon amido groups in protein, as well as a gene which encodes the enzyme, a recombinant vector which contains the gene, a transformant transformed with the vector and a method in which the transformant is cultured in a medium to effect production of the protein-deamidating enzyme and then the protein-deamidating enzyme is collected from the culture mixture.

    摘要翻译： 一种生产酶的方法，其包括在培养基中培养属于分类为细胞毒细菌或放线菌的细菌的菌株或新型细菌杆菌属（Chryseobacterium sp。）。 属于Chryseobacterium属的No.9670，具有产生蛋白质酰胺化氨基化性能的酶的能力，从而可以实现酶的生成，从而从培养液中收集酶， 使用直接作用于蛋白质中的酰胺基的新型酶的蛋白质，以及编码酶的基因，含有该基因的重组载体，用该载体转化的转化体和将该转化体培养于其中的方法 用于产生蛋白质脱酰胺酶的培养基，然后从培养物中收集蛋白质脱酰胺酶。

    Protein-deamidating enzyme, gene encoding the same, production process therefor, and use thereof
    17.
    发明授权
    Protein-deamidating enzyme, gene encoding the same, production process therefor, and use thereof 有权
    蛋白质脱酰胺酶，编码基因的基因，其制备方法及其用途

    公开(公告)号：US06251651B1

    公开(公告)日：2001-06-26

    申请号：US09324910

    申请日：1999-06-03

    申请人： Shotaro Yamaguchi ,  Akira Matsuura

    发明人： Shotaro Yamaguchi ,  Akira Matsuura

    IPC分类号： C12N900

    CPC分类号： A23J3/34 ,  C12N9/80

    摘要： A novel enzyme which has an activity to release side chain carboxyl groups and ammonia from a protein by acting upon side chain amido groups in the protein. This invention relates to a method for the production of an enzyme, which comprises culturing in a medium a strain that belongs to a bacterium classified into Cytophagales or Actinomycetes and has the ability to produce an enzyme having a property to deamidate amido groups in protein, thereby effecting production of said enzyme, and subsequently collecting said enzyme from the culture mixture. It also relates to a method for the modification of protein making use of a novel enzyme which directly acts upon amido groups in protein as well as to an enzyme which has a property to deamidate amido groups in protein and a gene which encodes said enzyme.

    摘要翻译： 一种具有通过作用于蛋白质中侧链酰胺基的蛋白质释放侧链羧基和氨的活性的新型酶。 本发明涉及一种酶的制备方法，其包括在培养基中培养属于细胞毒细菌或放线菌属的细菌的菌株，并且具有产生具有使蛋白质中酰氨基化的性质的酶的能力，由此 影响所述酶的产生，并随后从培养混合物中收集所述酶。 本发明还涉及使用直接作用于蛋白质中的酰胺基的新型酶的蛋白质的制造方法，以及具有使蛋白质中的酰氨基脱氨基化的酶和编码所述酶的基因的酶。

    Ascorbate oxidase, gene encoding the same, process for producing the same, and reagent composition using the same
    18.
    发明授权
    Ascorbate oxidase, gene encoding the same, process for producing the same, and reagent composition using the same 失效
    抗坏血酸氧化酶，编码其的基因，其制备方法和使用其的试剂组合物

    公开(公告)号：US5612208A

    公开(公告)日：1997-03-18

    申请号：US439114

    申请日：1995-05-11

    申请人： Yuji Nakanishi ,  Hitoshi Amano ,  Shotaro Yamaguchi

    发明人： Yuji Nakanishi ,  Hitoshi Amano ,  Shotaro Yamaguchi

    IPC分类号： A23L3/3571 ,  C12N9/02 ,  C12N15/53 ,  A23J1/00 ,  C12Q1/26

    CPC分类号： C12Y110/03003 ,  A23L3/3571 ,  C12N9/0063

    摘要： The present invention provides a novel ascorbate oxidase (ASOD) which catalyzes oxidation reaction of L-ascorbic acid with molecular oxygen to form L-dehydroascorbic acid and hydrogen peroxide, a process for producing the ascorbate oxidase comprising using a microorganism belonging to the genus Eupenicillium, a gene encoding ASOD, a transformant containing such a gene, a process for producing ASOD using such a transformant, and a reagent composition comprising ASOD, such as a reagent composition for examination, a food additive, and a reagent composition in the fields of food and clinical examination. The ascorbate oxidase has excellent stability particularly in a liquid state.

    摘要翻译： 本发明提供一种新型的抗坏血酸氧化酶（ASOD），其催化L-抗坏血酸与分子氧的氧化反应以形成L-脱氢抗坏血酸和过氧化氢，一种生产抗坏血酸氧化酶的方法，包括使用属于青霉属的微生物， 编码ASOD的基因，含有该基因的转化体，使用这种转化体生产ASOD的方法，以及包含ASOD的试剂组合物，例如食品添加剂和试剂组合物，例如食品中的试剂组合物 和临床检查。 抗坏血酸氧化酶具有优异的稳定性，特别是在液体状态下。

    METHOD FOR DESIGNING MUTANT ENZYME, METHOD FOR PREPARING MUTANT ENZYME, AND MUTANT ENZYME
    20.
    发明申请
    METHOD FOR DESIGNING MUTANT ENZYME, METHOD FOR PREPARING MUTANT ENZYME, AND MUTANT ENZYME 有权

    公开(公告)号：US20110165605A1

    公开(公告)日：2011-07-07

    申请号：US13062798

    申请日：2009-08-12

    申请人： Ryota Hashizume ,  Bunzo Mikami ,  Hirotaka Matsubara ,  Akiko Matsunaga ,  Shotaro Yamaguchi

    发明人： Ryota Hashizume ,  Bunzo Mikami ,  Hirotaka Matsubara ,  Akiko Matsunaga ,  Shotaro Yamaguchi

    IPC分类号： C12Q1/34 ,  C12N9/00 ,  C12N9/80 ,  C12N9/78 ,  C07H21/04 ,  C12N1/00

    CPC分类号： C12N9/80 ,  C12N9/48

    摘要： An object is to provide a novel method of improving an enzyme capable of deamidating a protein. A mutant enzyme is designed by the following steps: (1) specifying one or more amino acids selected from the following group, namely, consisting of an amino acid corresponding to the amino acid at position 35, an amino acid corresponding to the amino acid at position 38, an amino acid corresponding to the amino acid at position 39, an amino acid corresponding to the amino acid at position 40, an amino acid corresponding to the amino acid at position 41, an amino acid corresponding to the amino acid at position 42, an amino acid corresponding to the amino acid at position 43, an amino acid corresponding to the amino acid at position 45, an amino acid corresponding to the amino acid at position 46, an amino acid corresponding to the amino acid at position 49, an amino acid corresponding to the amino acid at position 79, an amino acid corresponding to the amino acid at position 80, an amino acid corresponding to the amino acid at position 81, an amino acid corresponding to the amino acid at position 82, an amino acid corresponding to the amino acid at position 83, an amino acid corresponding to the amino acid at position 84, an amino acid corresponding to the amino acid at position 103, an amino acid corresponding to the amino acid at position 104, an amino acid corresponding to the amino acid at position 105, an amino acid corresponding to the amino acid at position 106, an amino acid corresponding to the amino acid at position 117, an amino acid corresponding to the amino acid at position 142, an amino acid corresponding to the amino acid at position 143, an amino acid corresponding to the amino acid at position 146, an amino acid corresponding to the amino acid at position 166, and an amino acid corresponding to the amino acid at position 185 in an amino acid sequence set forth in SEQ ID NO: 2, in a protein deamidase (an enzyme to be mutated); and (2) constructing an amino acid sequence having substitution of the amino acid(s) specified in the step (1) by another amino acid(s) or having deletion of the amino acid(s) specified in the step (1) using the amino acid sequence for an enzyme to be mutated as a base sequence.