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公开(公告)号：US20140068875A1
公开(公告)日：2014-03-13
申请号：US14116757
申请日：2012-05-10
申请人： Yoshihiko Hirose , Yukihide Sato
发明人： Yoshihiko Hirose , Yukihide Sato
CPC分类号： A61K8/99 , A61K8/66 , A61Q5/10 , C12N9/001 , C12N9/0057 , C12N9/0059 , C12N9/0061 , C12N9/0063 , C12N9/0071 , C12P13/00 , C12Y103/03005 , C12Y110/03001 , C12Y110/03002 , C12Y110/03003 , C12Y110/03004 , C12Y114/18001 , D06P1/32 , D06P3/14
摘要： The object is to provide a process for dyeing that can provide an excellent dyeing effect while utilizing an oxidase, and a dyeing agent for use in the process. Provided is a dyeing agent comprising a modified enzyme obtained by adding positive charge by a chemical modification with an amine to an enzyme selected from the group consisting of an enzyme specified as EC 1.10.3.1, an enzyme specified as EC 1.10.3.2, an enzyme specified as EC 1.3.3.5, an enzyme specified as EC 1.10.3.4, an enzyme specified as EC 1.10.3.3 and an enzyme specified as EC 1.14.18.1. A process for dyeing using the dyeing agent is also provided.
摘要翻译： 本发明的目的是提供一种可以在使用氧化酶时提供优异的染色效果的染色方法和用于该方法的染色剂。 提供了一种染色剂，其包含通过化学修饰将正电荷与胺相加的酶所选择的酶所选择的酶，所述酶选自指定为EC1.10.3.1的酶，规定为EC1.10.3.2的酶，酶 规定为EC 1.3.3.5，规定为EC 1.10.3.4的酶，指定为EC 1.10.3.3的酶和指定为EC 1.14.18.1的酶。 还提供了使用染色剂进行染色的方法。
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公开(公告)号：US09546363B2
公开(公告)日：2017-01-17
申请号：US14399983
申请日：2013-05-23
发明人： Kenta Kinjo , Tomoko Aratake
CPC分类号： C12N9/96 , C12N9/0063 , C12Q1/26 , C12Y110/03003 , G01N2333/90235
摘要： Provided are: a method for stabilizing an ascorbic acid oxidase; a method for preserving an ascorbic acid oxidase; and a stabilized composition of an ascorbic acid oxidase. A method for stabilizing an ascorbic acid oxidase and a method for preserving an ascorbic acid oxidase, each of the methods comprising allowing an ascorbic acid oxidase to coexist with nitrous acid or a salt thereof, or a nitrous acid ester in an aqueous medium; and a stabilized composition of an ascorbic acid oxidase, which comprises an ascorbic acid oxidase being allowed to coexist with nitrous acid or a salt thereof, or a nitrous acid ester in an aqueous medium. The method for stabilizing an ascorbic acid oxidase, the method for preserving an ascorbic acid oxidase, and the stabilized composition of an ascorbic acid oxidase according to the present invention are useful for clinical diagnosis and the like.
摘要翻译： 提供：稳定抗坏血酸氧化酶的方法; 一种保存抗坏血酸氧化酶的方法; 和稳定的抗坏血酸氧化酶组合物。 用于稳定抗坏血酸氧化酶的方法和保存抗坏血酸氧化酶的方法，每种方法包括使抗坏血酸氧化酶与含水介质中的亚硝酸或其盐或亚硝酸酯共存; 和抗坏血酸氧化酶的稳定化组合物，其包含使抗坏血酸氧化酶与含水介质中的亚硝酸或其盐或亚硝酸酯共存。 用于稳定抗坏血酸氧化酶的方法，保存抗坏血酸氧化酶的方法和本发明的抗坏血酸氧化酶稳定化组合物可用于临床诊断等。
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3.发明授权
公开(公告)号：US4451487A
公开(公告)日：1984-05-29
申请号：US377277
申请日：1982-05-12
申请人： Hellmuth Vetter , Peter Scheibe , Waldemar Thum , Gotthilf Naher
发明人： Hellmuth Vetter , Peter Scheibe , Waldemar Thum , Gotthilf Naher
IPC分类号： A23J1/00 , A61K38/22 , A61K38/43 , A61K38/44 , A61K39/395 , C07K1/30 , C12N9/00 , C12N9/02 , C12N9/08 , C12N9/18 , C12N9/20 , C12P21/00
CPC分类号： C12Y110/03003 , A23J1/00 , C07K1/30 , C12N9/00 , C12N9/0063 , C12N9/0065 , C12N9/18 , C12N9/20 , Y10S435/814
摘要： The present invention provides a process for the purification or enrichment of biologically active proteins by the addition of a selective precipitation agent, wherein an aqueous solution containing the desired protein is mixed at a pH value below its isoelectric point with such an amount of an arylsulphonic acid azo dyestuff with two sulphonate groups, which are separated from one another by 8 to 10 double bonds standing in resonance relationship, among which there is present at least one N.dbd.N double bond, that at least the greater part of the biological activity of the desired active protein is present in the precipitate, the precipitate is separated off and the active protein is again brought into solution at a pH value above its isoelectric point.The present invention also provides an agent for the selective precipitation of biologically active proteins from aqueous solutions thereof without the loss of the biological activity, wherein it contains or consists of an aryl sulphonic acid azo dyestuff with two sulphonate groups, which are separated from one another by 8 to 10 double bonds standing in resonance relationship, among which there is present at least one N.dbd.N double bond.
摘要翻译： 本发明提供了通过添加选择性沉淀剂来纯化或富集生物活性蛋白质的方法，其中将含有所需蛋白质的水溶液在低于其等电点的pH值下与这样一定量的芳基磺酸 具有两个磺酸根基团的偶氮染料彼此分离，共8个至10个共振关系的双键，其中存在至少一个N = N双键，至少有大部分生物活性 所需的活性蛋白质存在于沉淀物中，分离出沉淀物，并将活性蛋白质再次以高于其等电点的pH值进入溶液中。 本发明还提供了用于从其水溶液中选择性沉淀生物活性蛋白质而不丧失生物活性的试剂，其中它包含或由具有两个磺酸盐基团的芳基磺酸偶氮染料组成，其彼此分离 共有8〜10个双键，其中存在至少一个N = N双键。
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公开(公告)号：US08740994B2
公开(公告)日：2014-06-03
申请号：US14116757
申请日：2012-05-10
申请人： Yoshihiko Hirose , Yukihide Sato
发明人： Yoshihiko Hirose , Yukihide Sato
CPC分类号： A61K8/99 , A61K8/66 , A61Q5/10 , C12N9/001 , C12N9/0057 , C12N9/0059 , C12N9/0061 , C12N9/0063 , C12N9/0071 , C12P13/00 , C12Y103/03005 , C12Y110/03001 , C12Y110/03002 , C12Y110/03003 , C12Y110/03004 , C12Y114/18001 , D06P1/32 , D06P3/14
摘要： The object is to provide a process for dyeing that can provide an excellent dyeing effect while utilizing an oxidase, and a dyeing agent for use in the process. Provided is a dyeing agent comprising a modified enzyme obtained by adding positive charge by a chemical modification with an amine to an enzyme selected from the group consisting of an enzyme specified as EC 1.10.3.1, an enzyme specified as EC 1.10.3.2, an enzyme specified as EC 1.3.3.5, an enzyme specified as EC 1.10.3.4, an enzyme specified as EC 1.10.3.3 and an enzyme specified as EC 1.14.18.1. A process for dyeing using the dyeing agent is also provided.
摘要翻译： 本发明的目的是提供一种可以在使用氧化酶时提供优异的染色效果的染色方法和用于该方法的染色剂。 提供了一种染色剂，其包含通过化学修饰将正电荷与胺相加的酶所选择的酶所选择的酶，所述酶选自指定为EC1.10.3.1的酶，规定为EC1.10.3.2的酶，酶 规定为EC 1.3.3.5，规定为EC 1.10.3.4的酶，指定为EC 1.10.3.3的酶和指定为EC 1.14.18.1的酶。 还提供了使用染色剂进行染色的方法。
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5.发明授权Ascorbate oxidase isolated from Acremonium sp. HI-25 ferm BP-3124 失效从ACREMONIUM SP分离的抗坏血酸氧化酶。 HI-25 FERM BP-3124
公开(公告)号：US5180672A
公开(公告)日：1993-01-19
申请号：US653709
申请日：1991-02-06
申请人： Homare Itoh , Takashi Shin , Takahisa Yajima , Shigeki Fukuyasu
发明人： Homare Itoh , Takashi Shin , Takahisa Yajima , Shigeki Fukuyasu
IPC分类号： A23C9/12 , A23L2/84 , A23L3/3436 , A23L3/3571 , A23L5/20 , C12N9/02 , C12R1/645
CPC分类号： C12Y110/03003 , A23L3/3571 , C12N9/0063 , Y10S435/822
摘要： An ascorbate oxidase having an optimum pH range of 3.5-4.5 is isolated from Acremonium sp. Hi-25 BP-3124. The ascorbate oxidase is used in foods or drinks to oxidize ascorbate to prevent damage to the foods or drinks by oxidation.
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公开(公告)号：US20150147798A1
公开(公告)日：2015-05-28
申请号：US14399983
申请日：2013-05-23
发明人： Kenta Kinjo , Tomoko Aratake
CPC分类号： C12N9/96 , C12N9/0063 , C12Q1/26 , C12Y110/03003 , G01N2333/90235
摘要： Provided are: a method for stabilizing an ascorbic acid oxidase; a method for preserving an ascorbic acid oxidase; and a stabilized composition of an ascorbic acid oxidase. A method for stabilizing an ascorbic acid oxidase and a method for preserving an ascorbic acid oxidase, each of the methods comprising allowing an ascorbic acid oxidase to coexist with nitrous acid or a salt thereof, or a nitrous acid ester in an aqueous medium; and a stabilized composition of an ascorbic acid oxidase, which comprises an ascorbic acid oxidase being allowed to coexist with nitrous acid or a salt thereof, or a nitrous acid ester in an aqueous medium. The method for stabilizing an ascorbic acid oxidase, the method for preserving an ascorbic acid oxidase, and the stabilized composition of an ascorbic acid oxidase according to the present invention are useful for clinical diagnosis and the like.
摘要翻译： 提供：稳定抗坏血酸氧化酶的方法; 一种保存抗坏血酸氧化酶的方法; 和稳定的抗坏血酸氧化酶组合物。 用于稳定抗坏血酸氧化酶的方法和保存抗坏血酸氧化酶的方法，每种方法包括使抗坏血酸氧化酶与含水介质中的亚硝酸或其盐或亚硝酸酯共存; 和抗坏血酸氧化酶的稳定化组合物，其包含使抗坏血酸氧化酶与含水介质中的亚硝酸或其盐或亚硝酸酯共存。 用于稳定抗坏血酸氧化酶的方法，保存抗坏血酸氧化酶的方法和本发明的抗坏血酸氧化酶稳定化组合物可用于临床诊断等。
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公开(公告)号：US06383533B1
公开(公告)日：2002-05-07
申请号：US09328435
申请日：1999-06-09
IPC分类号： A23L33571
CPC分类号： C12Y101/03004 , A21D2/268 , A21D8/042 , A23J3/16 , A23J3/341 , A23J3/346 , A23L7/109 , A23L13/46 , A23L13/74 , A23L15/25 , A23L17/65 , A23L17/70 , A23L29/06 , C12Y110/03003 , C12Y111/01006 , C12Y203/02013
摘要： A food containing a protein treated with at least one transglutaminase and at least one oxidoreductase. The presence of the treated protein enhances the quality of the food.
摘要翻译： 含有用至少一种转谷氨酰胺酶和至少一种氧化还原酶处理的蛋白质的食物。 经处理的蛋白质的存在增强了食物的质量。
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公开(公告)号：US5834273A
公开(公告)日：1998-11-10
申请号：US266097
申请日：1994-06-24
申请人： Masayuki Futatsugi , Kenji Gushi
发明人： Masayuki Futatsugi , Kenji Gushi
CPC分类号： C12Y110/03003 , C12N9/0046 , C12N9/0063 , C12N9/12 , C12N9/78 , C12N9/96
摘要： A modified enzyme obtained by modifying an enzyme such as a hydrogen peroxide-generating enzyme, ascorbate oxidase, etc. with a polysaccharide, polyamino acid or synthetic polymer having a plurality of carboxyl groups via a crosslinking agent capable of binding both carboxyl group and amino group is remarkably improved in resistance to heat, proteases, etc. and in storage stability in an aqueous solution.
摘要翻译： 通过能够结合羧基和氨基的交联剂，通过用多糖，聚氨基酸或具有多个羧基的合成聚合物改性酶，例如产生过氧化氢的酶，抗坏血酸氧化酶等获得的修饰酶 在耐热性，蛋白酶等方面以及在水溶液中的储存稳定性方面显着提高。
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公开(公告)号：US4331763A
公开(公告)日：1982-05-25
申请号：US217178
申请日：1980-12-16
申请人： Eiji Matsumura , Hidehiko Ishikawa , Hideo Misaki
发明人： Eiji Matsumura , Hidehiko Ishikawa , Hideo Misaki
CPC分类号： C12Y110/03003 , C12N9/0063
摘要： Ascorbate oxidase is produced by extraction from plants of the genus Sechium, particularly the species thereof which is Sechium edule Sw. The crushed plant tissues are extracted with an aqueous alkaline solvent, preferably at about pH 11. The extract is then subjected to centrifugation, concentration under vacuum, salting out with ammonium sulfate and solvent fractionation with acetone, to prepare crude ascorbate oxidase, which is then further purified by dialysis, ion exchange chromatography, adsorption chromatography and gel filtration. The ascorbate oxidase thus obtained has an optimum pH of about 7, a km value of 0.3 mM, an isoelectric point of around pH 6.3 and a molecular weight of about 100,000.
摘要翻译： 抗坏血酸氧化酶通过从Sechium属植物中提取而产生，特别是Sechium edule Sw的种类。 粉碎的植物组织用碱性水溶液萃取，优选在约pH 11下。然后将提取物进行离心，在真空下浓缩，用硫酸铵盐析并用丙酮溶剂分级，制备粗抗坏血酸氧化酶 进一步通过透析，离子交换层析，吸附层析和凝胶过滤纯化。 由此获得的抗坏血酸氧化酶具有约7的最佳pH，0.3mM的km值，约6.3的等电点和约100,000的分子量。
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公开(公告)号：US4160696A
公开(公告)日：1979-07-10
申请号：US716921
申请日：1976-08-23
申请人： Tai-Wing Wu
发明人： Tai-Wing Wu
CPC分类号： C12Y110/03003 , C12N9/0063 , C12Q1/26
摘要： Ascorbic acid in a sample such as blood serum is quantitatively assayed by converting the ascorbic acid to dehydroascorbic acid with ascorbic acid oxidase and reacting the dehydroascorbic with a coupling substance such as phenylenediamine to produce a detectable product. The ascorbic acid oxidase and coupling substance can be combined to form a reagent composition which can be incorporated into integral multilayer elements to form a test element.
摘要翻译： 通过用抗坏血酸氧化酶将抗坏血酸转化为脱氢抗坏血酸并使脱氢抗坏血酸与偶联物质如苯二胺反应以产生可检测的产物，定量测定样品如血清中的抗坏血酸。 抗坏血酸氧化酶和偶联物质可以组合形成试剂组合物，其可以并入整合的多层元件中以形成测试元件。
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