公开(公告)号：US20060024743A1

公开(公告)日：2006-02-02

申请号：US11241121

申请日：2005-09-29

申请人： Richard Mathies ,  Alexander Glazer ,  Jingyue Ju

发明人： Richard Mathies ,  Alexander Glazer ,  Jingyue Ju

IPC分类号： C12Q1/68 ,  C07K14/47 ,  C07H21/04

CPC分类号： G01N33/582 ,  C12Q1/6818 ,  C12Q1/6869 ,  G01N33/542 ,  C12Q2537/143 ,  C12Q2565/101 ,  C12Q2535/101 ,  C12Q2563/107 ,  C12Q2565/1015

摘要： Fluorescent labels having at least one donor and at least one acceptor fluorophore bonded to a polymeric backbone in energy transfer relationship, as well as methods for their use, are provided. Of particular interest are the subject labels wherein the polymeric backbone is a nucleic acid and the donor fluorophore is bonded to the 5′ terminus of said nucleic acid. Such labels find use as primers in applications involving nucleic acid chain extension, such as sequencing, PCR and the like.

公开(公告)号：US20050019902A1

公开(公告)日：2005-01-27

申请号：US10918575

申请日：2004-08-14

申请人： Richard Mathies ,  Eric Lagally ,  Peter Simpson

发明人： Richard Mathies ,  Eric Lagally ,  Peter Simpson

IPC分类号： B01J19/00 ,  B01L3/00 ,  B01L7/00 ,  C12M1/38

CPC分类号： B01L3/5027 ,  B01J19/0093 ,  B01L3/502723 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/502746 ,  B01L3/502753 ,  B01L7/52 ,  B01L2200/027 ,  B01L2200/0621 ,  B01L2200/0684 ,  B01L2200/10 ,  B01L2200/147 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2300/0874 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2300/1838 ,  B01L2300/1844 ,  B01L2400/0415 ,  B01L2400/0421 ,  B01L2400/0442 ,  B01L2400/0481 ,  B01L2400/0487 ,  B01L2400/049 ,  B01L2400/0605 ,  B01L2400/0611 ,  B01L2400/0633 ,  B01L2400/0683

摘要： A miniature device has a body including one, two or more reaction chambers. The reaction chambers are constructed for one or more of the following: sample acquisition, preparation or analysis. Preferably, a sample preparation reaction includes nucleic acid extraction, amplification, nucleic acid fragmentation, labeling, extension or a transcription.

摘要翻译： 微型装置具有包括一个，两个或更多个反应室的主体。 反应室构建为以下一个或多个：样品采集，制备或分析。 优选地，样品制备反应包括核酸提取，扩增，核酸碎裂，标记，延伸或转录。

公开(公告)号：US6028190A

公开(公告)日：2000-02-22

申请号：US646861

申请日：1996-05-08

申请人： Richard Mathies ,  Alexander Glazer ,  Jingyue Ju

发明人： Richard Mathies ,  Alexander Glazer ,  Jingyue Ju

IPC分类号： C12Q1/68 ,  G01N33/542 ,  G01N33/58 ,  C07H21/02

CPC分类号： G01N33/582 ,  C12Q1/6818 ,  C12Q1/6869 ,  G01N33/542

摘要： Fluorescent labels having at least one donor and at least one acceptor fluorophore bonded to a polymeric backbone in energy transfer relationship, as well as methods for their use, are provided. Of particular interest are the subject labels wherein the polymeric backbone is a nucleic acid and the donor fluorophore is bonded to the 5' terminus of said nucleic acid. Such labels find use as primers in applications involving nucleic acid chain extension, such as sequencing, PCR and the like.

公开(公告)号：US5869255A

公开(公告)日：1999-02-09

申请号：US790813

申请日：1997-01-30

申请人： Richard Mathies ,  Alexander Glazer ,  Jingyue Ju

发明人： Richard Mathies ,  Alexander Glazer ,  Jingyue Ju

IPC分类号： C12Q1/68 ,  G01N33/542

CPC分类号： G01N33/542 ,  C12Q1/6818 ,  C12Q1/6869

摘要： Compositions are provided comprising sets of fluorescent labeled oligonucleotides carrying pairs of donor and acceptor dye molecules, designed for efficient excitation of the donors at a single wavelength and emission from the acceptor in each of the pairs at different wavelengths. The different molecules having different donor-acceptor pairs can be modified to have substantially the same mobility and enhanced emission intensities under separation conditions, by varying the distance between the donor and acceptor in a given pair. Particularly, the fluorescent compositions find use as labels in analyzing double stranded and single stranded nucleic acid fragments using capillary electrophoresis.

摘要翻译： 提供的组合物包括携带供体和受体染料分子对的荧光标记寡核苷酸组，设计用于在单个波长下有效激发供体，并以不同波长的每对中的受体发射。 具有不同供体 - 受体对的不同分子可以通过改变在给定对中的供体和受体之间的距离而在分离条件下具有基本上相同的迁移率和增强的发射强度。 特别地，荧光组合物用于使用毛细管电泳分析双链和单链核酸片段的标签。