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    METHOD OF PRODUCING SUCCINIC ACID AND OTHER CHEMICALS USING SUCROSE-CONTAINING FEEDSTOCK
    12.
    发明申请
    METHOD OF PRODUCING SUCCINIC ACID AND OTHER CHEMICALS USING SUCROSE-CONTAINING FEEDSTOCK 有权
    使用含蔗糖的饲料生产农药和其他化学品的方法

    公开(公告)号：US20130337519A1

    公开(公告)日：2013-12-19

    申请号：US13993150

    申请日：2011-12-13

    申请人： Sudhandshu Dole ,  R. Rogers Yocum ,  Theron Hermann ,  Xiaohui Yu

    发明人： Sudhandshu Dole ,  R. Rogers Yocum ,  Theron Hermann ,  Xiaohui Yu

    IPC分类号： C12P7/46 ,  C12R1/01

    CPC分类号： C12R1/01 ,  C12N9/1051 ,  C12N9/16 ,  C12P7/18 ,  C12P7/42 ,  C12P7/46 ,  C12Y301/03024

    摘要： This invention relates to the production of chemicals by fermentation with a microorganism in which the fermentation medium contains the sugar sucrose. As a specific example, succinic acid is produced from a sucrose-containing renewable feedstock through fermentation using a biocatalyst. Examples of such a biocatalyst include microorganisms that have been enhanced in their ability to utilize sucrose as a carbon and energy source. The biocatalysts of the present invention are derived from the genetic manipulation of parental strains that were originally constructed with the goal to produce one or more chemicals (for example succinic acid and/or a salt of succinic acid) at a commercial scale using feedstocks other than sucrose. The genetic manipulations of the present invention involve the introduction of exogenous genes involved in the transport and metabolism of sucrose into the parental strains. The genes involved in the transport and metabolism of sucrose can also be introduced into a microorganism prior to developing the organism to produce a particular chemical. The genes involved in the transport and metabolism of sucrose can also be used to augment or improve the sucrose transport and metabolism by strains already known to have some ability for sucrose utilization in biological fermentation.

    摘要翻译： 本发明涉及通过用发酵培养基含有蔗糖的微生物进行发酵来生产化学品。 作为具体实例，使用生物催化剂通过发酵从含蔗糖的可再生原料生产琥珀酸。 这种生物催化剂的实例包括增强其利用蔗糖作为碳和能源的能力的微生物。 本发明的生物催化剂来源于最初以以商业规模生产一种或多种化学物（例如琥珀酸和/或琥珀酸盐）的目的而构建的亲本菌株的遗传操作，其使用除了 蔗糖。 本发明的遗传操作涉及将参与蔗糖转运和代谢的外源基因导入亲本菌株。 参与蔗糖运输和代谢的基因也可以在开发生物体之前被引入微生物以产生特定的化学物质。 参与蔗糖运输和代谢的基因也可用于增加或改善已知在生物发酵中具有蔗糖利用能力的菌株的蔗糖转运和代谢。

    Method of producing succinic acid and other chemicals using sucrose-containing feedstock
    14.
    发明授权
    Method of producing succinic acid and other chemicals using sucrose-containing feedstock 有权

    公开(公告)号：US09845513B2

    公开(公告)日：2017-12-19

    申请号：US13993150

    申请日：2011-12-13

    申请人： Sudhandshu Dole ,  R. Rogers Yocum ,  Theron Hermann ,  Xiaohui Yu

    发明人： Sudhandshu Dole ,  R. Rogers Yocum ,  Theron Hermann ,  Xiaohui Yu

    IPC分类号： C12P7/46 ,  C12R1/01 ,  C12N9/10 ,  C12N9/16 ,  C12P7/18 ,  C12P7/42

    CPC分类号： C12R1/01 ,  C12N9/1051 ,  C12N9/16 ,  C12P7/18 ,  C12P7/42 ,  C12P7/46 ,  C12Y301/03024

    摘要： This invention relates to the production of chemicals by fermentation with a microorganism in which the fermentation medium contains the sugar sucrose. As a specific example, succinic acid is produced from a sucrose-containing renewable feedstock through fermentation using a biocatalyst. Examples of such a biocatalyst include microorganisms that have been enhanced in their ability to utilize sucrose as a carbon and energy source. The biocatalysts of the present invention are derived from the genetic manipulation of parental strains that were originally constructed with the goal to produce one or more chemicals (for example succinic acid and/or a salt of succinic acid) at a commercial scale using feedstocks other than sucrose. The genetic manipulations of the present invention involve the introduction of exogenous genes involved in the transport and metabolism of sucrose into the parental strains. The genes involved in the transport and metabolism of sucrose can also be introduced into a microorganism prior to developing the organism to produce a particular chemical. The genes involved in the transport and metabolism of sucrose can also be used to augment or improve the sucrose transport and metabolism by strains already known to have some ability for sucrose utilization in biological fermentation.

    FERMENTATION PROCESS FOR THE PRODUCTION OF ORGANIC ACIDS
    15.
    发明申请
    FERMENTATION PROCESS FOR THE PRODUCTION OF ORGANIC ACIDS 审中-公开
    用于生产有机酸的发酵方法

    公开(公告)号：US20130130339A1

    公开(公告)日：2013-05-23

    申请号：US13812886

    申请日：2011-07-30

    申请人： Theron Hermann ,  James Reinhardt ,  Xiaohui Yu ,  Russell Udani ,  Lauren Staples

    发明人： Theron Hermann ,  James Reinhardt ,  Xiaohui Yu ,  Russell Udani ,  Lauren Staples

    IPC分类号： C12P7/46

    CPC分类号： C12P7/46

    摘要： This invention relates to improvements in the fermentation process used in the production of organic acids from biological feedstock using bacterial catalysts. The improvements in the fermentation process involve providing a fermentation medium comprising an appropriate form of inorganic carbon, an appropriate amount of aeration and a biocatalyst with an enhanced ability to uptake and assimilate the inorganic carbon into the organic acids. This invention also provides, as a part of an integrated fermentation facility, a novel process for producing a solid source of inorganic carbon by sequestering carbon released from the fermentation in an alkali solution.

    摘要翻译： 本发明涉及使用细菌催化剂从生物原料生产有机酸的发酵方法的改进。 发酵过程的改进包括提供包含适当形式的无机碳，适量曝气和生物催化剂的发酵培养基，其具有增强吸收和吸收无机碳进入有机酸的能力。 作为一体化发酵设备的一部分，本发明还提供了一种通过在碱溶液中从发酵中释放的碳进行螯合来生产无机碳固体源的新方法。

    Microorganisms and processes for enhanced production of pantothenate
    16.
    发明申请
    Microorganisms and processes for enhanced production of pantothenate 审中-公开
    用于增加泛酸盐生产的微生物和方法

    公开(公告)号：US20060099692A1

    公开(公告)日：2006-05-11

    申请号：US10520210

    申请日：2002-07-03

    申请人： R Yocum ,  Thomas Patterson ,  Janice Pero ,  Theron Hermann

    发明人： R Yocum ,  Thomas Patterson ,  Janice Pero ,  Theron Hermann

    IPC分类号： C12P19/12 ,  C12P13/04 ,  C12N1/21

    CPC分类号： C07H21/04 ,  C12N9/0006 ,  C12N9/1003 ,  C12N9/1014 ,  C12N15/52 ,  C12P13/02

    摘要： The present invention features improved methods for the enhanced production of pantoate and pantothenate utilizing microorganisms having modified pantothenate biosynthetic enzyme activities and having modified methylenetetrahydrofolate (MTF) biosynthetic enzyme activities. In particular, the invention features methods for enhancing production of desired products by increasing levels of a key intermediate, ketopantoate, by increasing enzymes or substrates that contribute directly or indirectly to its synthesis. Recombinant microorganisms and conditions for culturing same are also featured. Also featured are compositions produced by such microorganisms.

    摘要翻译： 本发明的特征在于利用具有改良的泛酸生物合成酶活性并具有改性的亚甲基四氢叶酸（MTF）生物合成酶活性的微生物增强生产泛酸盐和泛酸盐的改进方法。 特别地，本发明的特征在于通过增加对其合成直接或间接有贡献的酶或底物来增加关键中间体酮托品酸的水平来增强所需产物的产生的方法。 还具有重组微生物和培养条件。 还有这些微生物产生的组合物。

    Microorganisms and processes for enhanced production of pantothenate
    17.
    发明申请
    Microorganisms and processes for enhanced production of pantothenate 有权
    用于增加泛酸盐生产的微生物和方法

    公开(公告)号：US20060141585A1

    公开(公告)日：2006-06-29

    申请号：US10520220

    申请日：2003-07-03

    申请人： R Yocum ,  Thomas Patterson ,  Janice Pero ,  Theron Hermann

    发明人： R Yocum ,  Thomas Patterson ,  Janice Pero ,  Theron Hermann

    IPC分类号： C12P13/04 ,  C12N1/21

    CPC分类号： C12N15/52 ,  C12P13/02

    摘要： The present invention features improved methods for the enhanced production of pantoate and pantothenate utilizing microorganisms having modified pantothenate biosynthetic enzyme activities and having modified methylenetetrahydrofolate (MTF) biosynthetic enzyme activities. In particular, the invention features methods for enhancing production of desired products by increasing levels of a key intermediate, ketopantoate, by increasing enzymes or substrates that contribute directly or indirectly to its synthesis. Recombinant microorganisms and conditions for culturing same are also are featured. Also featured are compositions produced by such microorganisms.

    摘要翻译： 本发明的特征在于利用具有改良的泛酸生物合成酶活性并具有改性的亚甲基四氢叶酸（MTF）生物合成酶活性的微生物增强生产泛酸盐和泛酸盐的改进方法。 特别地，本发明的特征在于通过增加对其合成直接或间接有贡献的酶或底物来增加关键中间体酮托品酸的水平来增强所需产物的产生的方法。 还具有重组微生物和培养条件。 还有这些微生物产生的组合物。

    Methods and microorganisms for production of panto-compounds
    18.
    发明申请
    Methods and microorganisms for production of panto-compounds 有权
    泛化合物生产方法和微生物

    公开(公告)号：US20050089973A1

    公开(公告)日：2005-04-28

    申请号：US10984449

    申请日：2004-11-08

    申请人： R. Yocum ,  Thomas Patterson ,  Theron Hermann ,  Janice Pero

    发明人： R. Yocum ,  Thomas Patterson ,  Theron Hermann ,  Janice Pero

    IPC分类号： C12N1/21 ,  C12N15/52 ,  C12P13/02 ,  C12P17/04 ,  C12P13/04 ,  C12P13/06

    CPC分类号： C12P17/04 ,  C12N15/52 ,  C12P13/02

    摘要： The present invention features methods of producing panto-compounds (e.g., pantothenate) using microorganisms in which the pantothenate biosynthetic pathway and/or the isoleucine-valine biosynthetic pathway and/or the coenzymeA biosynthetic pathway has been manipulated. Methods featuring ketopantoate reductase overexpressing microorganisms as well as aspartate α-decarboxylase overexpressing microorganisms are provided. Methods of producing panto-compounds in a precursor-independent manner and in high yield are described. Recombinant microorganisms, vectors, isolated nucleic acid molecules, genes and gene products useful in practicing the above methodologies are also provided. The present invention also features a previously unidentified microbial pantothenate kinase gene, coaX, as well as methods of producing panto-compounds utilizing microorganisms having modified pantothenate kinase activity. Recombinant microorganisms, vectors, isolated coaX nucleic acid molecules and purified CoaX proteins are featured. Also featured are methods for identifying pantothenate kinase modulators utilizing the recombinant microorganisms and/or purified CoaX proteins of the present invention.

    摘要翻译： 本发明的特征在于使用其中泛酸生物合成途径和/或异亮氨酸 - 缬氨酸生物合成途径和/或辅酶A生物合成途径已经被操纵的微生物来生产泛化合物（例如泛酸盐）的方法。 本发明提供了酮过氧还原酶过表达微生物以及天冬氨酸α-脱羧酶过表达微生物的方法。 描述了以前体非依赖性方式和高产率生产泛化合物的方法。 还提供了用于实践上述方法的重组微生物，载体，分离的核酸分子，基因和基因产物。 本发明还具有以前未鉴定的微生物泛酸激酶基因coaX，以及利用具有改良的泛酸激酶活性的微生物产生泛化合物的方法。 重组微生物，载体，分离的coaX核酸分子和纯化的CoaX蛋白。 还特征在于利用本发明的重组微生物和/或纯化的CoaX蛋白鉴定泛酸激酶调节剂的方法。