公开(公告)号：US20130011873A1

公开(公告)日：2013-01-10

申请号：US13611827

申请日：2012-09-12

申请人： Seiichi HARA ,  Kenzo Yokozeki ,  Isao Abe ,  Naoto Tonouchi ,  Yasuko Jojima

发明人： Seiichi HARA ,  Kenzo Yokozeki ,  Isao Abe ,  Naoto Tonouchi ,  Yasuko Jojima

IPC分类号： C12P21/06 ,  C12N15/63 ,  C12N9/00 ,  C12N1/19 ,  C12N1/15 ,  C12N15/52 ,  C12N1/21

CPC分类号： C12N9/00 ,  C12N9/93 ,  C12P21/00 ,  C12P21/02

摘要： DNA and recombinant DNA that encode a peptide-forming enzyme, a method for producing a peptide-forming enzyme, and a method for producing a dipeptide are disclosed. A method for producing a dipeptide includes producing a dipeptide from a carboxy component and an amine component by using a culture of a microbe belonging to the genus Sphingobacterium and having the ability to form the dipeptide from the carboxy component and the amine component, a microbial cell separated from the culture, treated microbial cell product of the microbe or a peptide-forming enzyme derived from the microbe.

公开(公告)号：US07736871B2

公开(公告)日：2010-06-15

申请号：US11841151

申请日：2007-08-20

申请人： Seiichi Hara ,  Kenzo Yokozeki ,  Isao Abe ,  Naoto Tonouchi ,  Yasuko Jojima

发明人： Seiichi Hara ,  Kenzo Yokozeki ,  Isao Abe ,  Naoto Tonouchi ,  Yasuko Jojima

IPC分类号： C12P21/06 ,  C12N9/00 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04

CPC分类号： C12N9/00 ,  C12N9/93 ,  C12P21/00 ,  C12P21/02

摘要： DNA and recombinant DNA that encode a peptide-forming enzyme, a method for producing a peptide-forming enzyme, and a method for producing a dipeptide are disclosed. A method for producing a dipeptide includes producing a dipeptide from a carboxy component and an amine component by using a culture of a microbe belonging to the genus Sphingobacterium and having the ability to form the dipeptide from the carboxy component and the amine component, a microbial cell separated from the culture, treated microbial cell product of the microbe or a peptide-forming enzyme derived from the microbe.

公开(公告)号：US20080050773A1

公开(公告)日：2008-02-28

申请号：US11841211

申请日：2007-08-20

申请人： Seiichi HARA ,  Kenzo Yokozeki ,  Isao Abe ,  Naoto Tonouchi ,  Yasuko Jojima

发明人： Seiichi HARA ,  Kenzo Yokozeki ,  Isao Abe ,  Naoto Tonouchi ,  Yasuko Jojima

IPC分类号： C12P21/06 ,  C12N9/00

CPC分类号： C12N9/00 ,  C12N9/93 ,  C12P21/00 ,  C12P21/02

摘要： DNA and recombinant DNA that encode a peptide-forming enzyme, a method for producing a peptide-forming enzyme, and a method for producing a dipeptide are disclosed. A method for producing a dipeptide includes producing a dipeptide from a carboxy component and an amine component by using a culture of a microbe belonging to the genus Sphingobacterium and having the ability to form the dipeptide from the carboxy component and the amine component, a microbial cell separated from the culture, treated microbial cell product of the microbe or a peptide-forming enzyme derived from the microbe.

公开(公告)号：US07288389B2

公开(公告)日：2007-10-30

申请号：US10855533

申请日：2004-05-28

申请人： Seiichi Hara ,  Kenzo Yokozeki ,  Isao Abe ,  Naoto Tonouchi ,  Yasuko Jojima

发明人： Seiichi Hara ,  Kenzo Yokozeki ,  Isao Abe ,  Naoto Tonouchi ,  Yasuko Jojima

IPC分类号： C12P21/06 ,  C12N9/48 ,  C12N9/52 ,  C12N1/20 ,  C07H21/04

CPC分类号： C12N9/00 ,  C12N9/93 ,  C12P21/00 ,  C12P21/02

摘要： DNA and recombinant DNA that encode a peptide-forming enzyme, a method for producing a peptide-forming enzyme, and a method for producing a dipeptide are disclosed. A method for producing a dipeptide includes producing a dipeptide from a carboxy component and an amine component by using a culture of a microbe belonging to the genus Sphingobacterium and having the ability to form the dipeptide from the carboxy component and the amine component, a microbial cell separated from the culture, treated microbial cell product of the microbe or a peptide-forming enzyme derived from the microbe.

摘要翻译： 公开了编码肽形成酶的DNA和重组DNA，产生肽形成酶的方法及其制备方法。 制备二肽的方法包括通过使用属于鞘氨醇杆菌属的微生物的培养物从羧基组分和胺组分制备二肽并具有从羧基组分和胺组分形成二肽的能力，微生物细胞 与培养物分离，处理微生物的微生物细胞产物或源自微生物的肽形成酶。

公开(公告)号：US06335177B1

公开(公告)日：2002-01-01

申请号：US09347001

申请日：1999-07-02

申请人： Yasuhiro Mihara ,  Sonoko Takeuchi ,  Yasuko Jojima ,  Naoto Tonouchi ,  Ryosuke Fudou ,  Kenzo Yokozeki

发明人： Yasuhiro Mihara ,  Sonoko Takeuchi ,  Yasuko Jojima ,  Naoto Tonouchi ,  Ryosuke Fudou ,  Kenzo Yokozeki

IPC分类号： C12P100

CPC分类号： C12R1/01 ,  C12P7/06 ,  C12P7/18 ,  C12P19/02 ,  Y02E50/17

摘要： According to the present invention, there are provided microorganisms having an ability to producing xylitol or D-xylulose by fermentation, and a method for producing xylitol or D-xylulose using the microorganisms. Osmophilic microorganisms were collected from soil, and the obtained microorganisms were searched for a bacterium having an ability to produce xylitol or D-xylulose from glucose. Xylitol or D-xylulose is produced by culturing an isolated bacterium in a suitable medium to accumulate xylitol or D-xylulose in the medium, and collecting xylitol or D-xylulose from the medium.

摘要翻译： 根据本发明，提供了通过发酵生产木糖醇或D-木酮糖的能力的微生物，以及使用微生物生产木糖醇或D-木酮糖的方法。 从土壤中收集渗透性微生物，并从所得微生物中搜索具有从葡萄糖产生木糖醇或D-木酮糖的能力的细菌。 通过在合适的培养基中培养分离的细菌以在培养基中积累木糖醇或D-木酮糖并从培养基中收集木糖醇或D-木酮糖来生产木糖醇或D-木酮糖。

公开(公告)号：US20100120172A1

公开(公告)日：2010-05-13

申请号：US12548898

申请日：2009-08-27

申请人： Naoyuki Fukuchi ,  Fumie Futaki ,  Morikazu Kito ,  Seiichi Sato ,  Takayuki Kajiura ,  Yukitsugu Ono ,  Koichi Ishii ,  Akiko Tanaka ,  Junko Shinozaki ,  Yasuko Jojima

发明人： Naoyuki Fukuchi ,  Fumie Futaki ,  Morikazu Kito ,  Seiichi Sato ,  Takayuki Kajiura ,  Yukitsugu Ono ,  Koichi Ishii ,  Akiko Tanaka ,  Junko Shinozaki ,  Yasuko Jojima

IPC分类号： G01N33/53

CPC分类号： C07K14/705 ,  C07K14/755 ,  C07K2317/52 ,  C07K2319/00 ,  C07K2319/30 ,  G01N33/86 ,  G01N2333/755

摘要： A method for conveniently detecting binding between the von Willebrand factor and glycoprotein Ib and a means to be used therein. The von Willebrand factor fixed in a reactor immobilized in a reaction vessel in the presence of bottrocetin is bound to a chimeric protein constructed by fusing the carboxyl terminal of a partial protein containing the von Willebrand factor-binding site of glycoprotein Ib with the amino terminal of the Fc region of an immunoglobulin molecule. Then the Fc region of the above immunoglobulin molecule is detected to thereby detect the binding between the von Willebrand factor and the glycoprotein Ib or inhibition of this binding.

摘要翻译： 一种方便检测血管性血友病因子与糖蛋白Ⅰb之间的结合的方法及其中使用的方法。 固定在反应容器中的存在于布可菌素的反应器中的血管性血友病因子结合于通过将含有糖蛋白1b的血管性血友病变因子结合位点的部分蛋白的羧基末端与羧基末端相连的氨基末端 免疫球蛋白分子的Fc区。 然后检测上述免疫球蛋白分子的Fc区，从而检测血管性血友病因子与糖蛋白Ib之间的结合或抑制该结合。

公开(公告)号：US07608695B2

公开(公告)日：2009-10-27

申请号：US10825127

申请日：2004-04-16

申请人： Naoyuki Fukuchi ,  Fumie Futaki ,  Morikazu Kito ,  Seiichi Sato ,  Takayuki Kajiura ,  Yukitsugu Ono ,  Koichi Ishii ,  Akiko Tanaka ,  Junko Shinozaki ,  Yasuko Jojima

发明人： Naoyuki Fukuchi ,  Fumie Futaki ,  Morikazu Kito ,  Seiichi Sato ,  Takayuki Kajiura ,  Yukitsugu Ono ,  Koichi Ishii ,  Akiko Tanaka ,  Junko Shinozaki ,  Yasuko Jojima

IPC分类号： C12P21/08

CPC分类号： C07K14/705 ,  C07K14/755 ,  C07K2317/52 ,  C07K2319/00 ,  C07K2319/30 ,  G01N33/86 ,  G01N2333/755

摘要： A method for conveniently detecting binding between the von Willebrand factor and glycoprotein Ib and a means to be used therein. The von Willebrand factor fixed in a reactor immobilized in a reaction vessel in the presence of bottrocetin is bound to a chimeric protein constructed by fusing the carboxyl terminal of a partial protein containing the von Willebrand factor-binding site of glycoprotein Ib with the amino terminal of the Fc region of an immunoglobulin molecule. Then the Fc region of the above immunoglobulin molecule is detected to thereby detect the binding between the von Willebrand factor and the glycoprotein Ib or inhibition of this binding.

摘要翻译： 一种方便检测血管性血友病因子与糖蛋白Ⅰb之间的结合的方法及其中使用的方法。 固定在反应容器中的存在于布可菌素的反应器中的血管性血友病因子结合于通过将含有糖蛋白1b的血管性血友病变因子结合位点的部分蛋白的羧基末端与羧基末端相连的氨基末端 免疫球蛋白分子的Fc区。 然后检测上述免疫球蛋白分子的Fc区，从而检测血管性血友病因子与糖蛋白Ib之间的结合或抑制该结合。