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    METHOD FOR MANUFACTURING CELLOOLIGOSACCHARIDE
    14.
    发明申请
    METHOD FOR MANUFACTURING CELLOOLIGOSACCHARIDE 审中-公开

    公开(公告)号：US20180362669A1

    公开(公告)日：2018-12-20

    申请号：US15779986

    申请日：2016-12-14

    Applicant: SHOWA DENKO K.K. ,  National University Corporation Hokkaido University

    Inventor： Ichiro FUJITA ,  Atsushi FUKUOKA ,  Hirokazu KOBAYASHI ,  Abhijit SHROTORI

    IPC: C08B15/02 ,  B09B3/00

    Abstract: A method of manufacturing a cellooligosaccharide containing an oligomer having a degree of polymerization of from 3 to 6, the method includes performing a reaction involving heating and hydrolyzing plant biomass in coexistence with a carbon catalyst and water under such a condition that a temperature-time product over a range of from 170° C. to 230° C. in a graph for showing a relationship between a reaction temperature on an axis of ordinate and a reaction time on an axis of abscissa is from 80° C.·min to 365° C.·min. The maximum reaction temperature (X) and the retention time (Y) at the maximum reaction temperature satisfy a relationship represented by the following expressions (1) to (3). Y>−0.0714X+15  (1) Y 175  (3)

    METHOD FOR TREATING CELLULOSE-CONTAINING BIOMASS
    16.
    发明申请
    METHOD FOR TREATING CELLULOSE-CONTAINING BIOMASS 审中-公开
    用于处理含细菌生物质的方法

    公开(公告)号：US20160251690A1

    公开(公告)日：2016-09-01

    申请号：US15027617

    申请日：2014-09-09

    Applicant: SHOWA DENKO K.K.

    Inventor： Ichiro FUJITA ,  Shinji YAMAKI ,  Tadashi YONEDA

    IPC: C12P19/14 ,  C12P19/02

    CPC classification number: C12P19/14 ,  C12M45/02 ,  C12P19/02 ,  C12P2201/00 ,  C13K1/02

    Abstract: A treatment method for biomass, which is a pretreatment method including continuously performing hydrothermal treatment using a screw extruder to produce a biomass composition for saccharification from cellulose-containing biomass and containing only a small quantity of furfural, the treatment method sequentially including: pulverizing the cellulose-containing biomass so as to have a maximum grain size of 1,000 μm or less and adjusting a water content ratio thereof to from 30% to 80%; performing hydrothermal treatment in which an element including a seal ring and at least one set of a kneading disc and/or a left-hand screw is arranged immediately upstream of the seal ring, at a temperature of (1) from 150° C. to 200° C. or (2) from 200° C. to 215° C. for 0.1 minute to 15 minutes while performing kneading pulverization having a grinding effect; and cooling a treated product to 100° C. or less to recover the treated product.

    Abstract translation: 一种生物质处理方法，其特征在于，所述预处理方法包括使用螺杆挤出机连续进行水热处理，以生产由含纤维素生物质糖化并仅含有少量糠醛的生物质组合物，所述处理方法依次包括：将所述纤维素 含有生物质，使其最大粒径为1000μm以下，将其含水率调整为30〜80％。 进行水热处理，其中包括密封环和至少一组捏合盘和/或左旋螺杆的元件紧邻密封环的上游，在（1）从150℃到 200℃或（2）从200℃至215℃进行0.1分钟至15分钟，同时进行具有研磨效果的捏合粉碎; 并将处理的产物冷却至100℃或更低以回收经处理的产物。

    METHOD FOR ASSAYING CELLULASE ACTIVITY, SCREENING METHOD USING ASSAYING METHOD, AND HIGH-PERFORMANCE CELLULASE-PRODUCING BACTERIA SELECTED USING SCREENING METHOD
    17.
    发明申请
    METHOD FOR ASSAYING CELLULASE ACTIVITY, SCREENING METHOD USING ASSAYING METHOD, AND HIGH-PERFORMANCE CELLULASE-PRODUCING BACTERIA SELECTED USING SCREENING METHOD 审中-公开
    用于测定纤维素酶活性的方法，使用测定方法的筛选方法和使用筛选方法选择的高性能纤维素酶生产细菌

    公开(公告)号：US20160244804A1

    公开(公告)日：2016-08-25

    申请号：US15044691

    申请日：2016-02-16

    Applicant: SHOWA DENKO K.K.

    Inventor： Ichiro FUJITA ,  Shinji YAMAKI ,  Tadashi YONEDA

    IPC: C12Q1/34 ,  C12N1/14 ,  C12R1/645

    CPC classification number: C12Q1/34 ,  C12R1/645 ,  G01N2333/942

    Abstract: A method for assaying cellulase activity, including: a process (A) of preparing two or more substrate solutions, which have an identical absorbance measured at an identical wavelength and in which cellulose is dispersed at an identical concentration, and measuring the absorbance of each of the substrate solutions; a process (B) of respectively adding different kinds of enzyme solutions to the substrate solutions, and performing an enzyme reaction under the same conditions; a process (C) of measuring the absorbance of each of the substrate solutions after the enzyme reaction; a process (D) of calculating the absorbance decrease values of the substrate solutions before and after the enzyme reaction; and a process (E) of assaying cellulase activities of the enzyme solutions based on the absorbance decrease values. In the assay of the process (E), it is determined that the enzyme solutions have higher cellulase activity as the absorbance decrease values become larger.

    Abstract translation: 一种测定纤维素酶活性的方法，包括：制备两种或多种底物溶液的方法（A），其具有在相同波长下测量的相同吸光度，其中纤维素以相同浓度分散，并测量每种 底物溶液; 分别将不同种类的酶溶液添加到底物溶液中的方法（B），并在相同条件下进行酶反应; 测量酶反应后每种底物溶液的吸光度的方法（C）; 计算酶反应前后底物溶液的吸光度降低值的方法（D）; 以及基于吸光度降低值测定酶溶液的纤维素酶活性的方法（E）。 在方法（E）的测定中，确定随着吸光度降低值变大，酶溶液具有较高的纤维素酶活性。

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