公开(公告)号：US5968793A

公开(公告)日：1999-10-19

申请号：US881687

申请日：1997-06-24

申请人： Zhan-Bin Liu ,  Joan Tellefsen Odell

发明人： Zhan-Bin Liu ,  Joan Tellefsen Odell

IPC分类号： A01H5/00 ,  C07K14/035 ,  C07K14/395 ,  C07K14/415 ,  C12N5/10 ,  C12N15/09 ,  C12N15/82 ,  C12N5/14 ,  A01H1/04 ,  A01H5/10 ,  C12N15/90

CPC分类号： C07K14/005 ,  C07K14/395 ,  C07K14/415 ,  C12N15/8217 ,  C12N2710/16622

摘要： A method for regulating gene expression in a stably transformed transgenic plant cell utilizing a Gal4 chimeric transcription factor is described.

摘要翻译： 描述了使用Gal4嵌合转录因子调节稳定转化的转基因植物细胞中的基因表达的方法。

公开(公告)号：US20080163396A1

公开(公告)日：2008-07-03

申请号：US11888497

申请日：2007-07-31

申请人： Stephen M. Allen ,  Quinn Qun Zhu ,  Zhan-Bin Liu

发明人： Stephen M. Allen ,  Quinn Qun Zhu ,  Zhan-Bin Liu

IPC分类号： C12N15/11 ,  C12N15/63 ,  C12N5/10 ,  A01H5/00

CPC分类号： C07K14/415 ,  C12N15/8216 ,  C12N15/8218

摘要： This invention relates to an isolated nucleic acid fragment encoding a protein involved in regulation of gene expression. The invention also relates to the construction of a chimeric gene encoding all or a portion of the protein involved in regulation of gene expression, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the protein involved in regulation of gene expression in a transformed host cell.

摘要翻译： 本发明涉及编码参与基因表达调节的蛋白质的分离的核酸片段。 本发明还涉及在正义或反义方向上编码涉及基因表达调控的全部或部分蛋白质的嵌合基因的构建，其中嵌合基因的表达导致生产参与调节的蛋白质水平的改变 在转化的宿主细胞中的基因表达。

公开(公告)号：US20140338070A1

公开(公告)日：2014-11-13

申请号：US14236597

申请日：2012-07-18

申请人： Andrew Mark Cigan ,  Saverio Carl Falco ,  Huirong Gao ,  Michael W. Lassner ,  Zhongsen Li ,  Zhan-Bin Liu ,  Leszek A. Lyznik ,  David J. Peterson ,  Christopher Jay Scelonge

发明人： Andrew Mark Cigan ,  Saverio Carl Falco ,  Huirong Gao ,  Michael W. Lassner ,  Zhongsen Li ,  Zhan-Bin Liu ,  Leszek A. Lyznik ,  David J. Peterson ,  Christopher Jay Scelonge

IPC分类号： C12N15/82

CPC分类号： C12N15/8213

摘要： Compositions and methods are provided for the targeted integration of a polynucleotide sequence of interest into the genome of a plant or plant cell. The methods and compositions employ recognition sites for endonucleases and endonucleases in combination with site-specific recombination sites/recombinases to provide an effective system for establishing target sites within the genome of a plant, plant cell or seed. Once such target sites are established, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest.

摘要翻译： 提供组合物和方法用于将目的多核苷酸序列靶向整合到植物或植物细胞的基因组中。 方法和组合物使用核酸内切酶和内切核酸酶的识别位点与位点特异性重组位点/重组酶组合，以提供用于在植物，植物细胞或种子的基因组内建立靶位点的有效系统。 一旦建立了这样的靶位点，就可以采用多种方法来进一步修饰靶位点，使得它们含有多种感兴趣的多核苷酸。

公开(公告)号：US20130263324A1

公开(公告)日：2013-10-03

申请号：US13427138

申请日：2012-03-22

申请人： Michael Lassner ,  Derek Jantz ,  James Jefferson Smith ,  Mark Cigan ,  Carl Falco ,  Huirong Gao ,  Zhongsen Li ,  Zhan-Bin Liu ,  Sergei Svitashev

发明人： Michael Lassner ,  Derek Jantz ,  James Jefferson Smith ,  Mark Cigan ,  Carl Falco ,  Huirong Gao ,  Zhongsen Li ,  Zhan-Bin Liu ,  Sergei Svitashev

IPC分类号： C12N15/82

CPC分类号： C12N15/8202 ,  A01H1/06 ,  C12N9/22 ,  C12N15/8213

摘要： Methods for producing in a plant a complex transgenic trait locus comprising at least two altered target sequences in a genomic region of interest are disclosed. The methods involve the use of two or more double-strand-break-inducing agents, each of which can cause a double-strand break in a target sequence in the genomic region of interest which results in an alteration in the target sequence. Also disclosed are complex transgenic trait loci in plants. A complex transgenic trait locus comprises at least two altered target sequences that are genetically linked to a polynucleotide of interest. Plants, plant cells, plant parts, and seeds comprising one or more complex transgenic trait loci are also disclosed.

摘要翻译： 公开了在植物中生产复合转基因性状基因座的方法，所述基因座包含在目的基因组区域中的至少两个改变的靶序列。 所述方法包括使用两种或更多种双链断裂诱导剂，其中每一种可引起目的基因组区域中靶序列中的双链断裂，导致目标序列的改变。 还公开了植物中的复杂的转基因性状基因座。 复杂的转基因性状基因座包含与感兴趣的多核苷酸遗传连锁的至少两个改变的靶序列。 还公开了植物，植物细胞，植物部分和包含一个或多个复合转基因性状基因座的种子。

公开(公告)号：US20080220143A1

公开(公告)日：2008-09-11

申请号：US11673843

申请日：2007-02-12

申请人： Anthony J. Kinney ,  Edgar Benjamin Cahoon ,  Howard Glenn Damude ,  William D. Hitz ,  Zhan-Bin Liu ,  Charles W. Kolar

发明人： Anthony J. Kinney ,  Edgar Benjamin Cahoon ,  Howard Glenn Damude ,  William D. Hitz ,  Zhan-Bin Liu ,  Charles W. Kolar

IPC分类号： A23D9/007 ,  A23L2/38 ,  A23L1/36 ,  A23K1/16 ,  A23L1/325 ,  A01H1/00

CPC分类号： A23K20/158 ,  A23L2/52 ,  A23L33/115 ,  A23L33/12

摘要： Oilseed plants which have been transformed to produce at least 8.0% arachidonic acid (ARA) as well as uses of oils and seeds obtained from such transformed plants in a variety of food and feed applications are described.

摘要翻译： 已经转化生产至少8.0％花生四烯酸（ARA）的油籽植物以及在各种食品和饲料应用中从这种转化植物获得的油和种子的用途被描述。

公开(公告)号：US07368633B2

公开(公告)日：2008-05-06

申请号：US10917602

申请日：2004-08-13

申请人： Saverio Carl Falco ,  Zhan-Bin Liu

发明人： Saverio Carl Falco ,  Zhan-Bin Liu

IPC分类号： C12N15/52 ,  C07H21/04 ,  C07K14/415 ,  C12N5/14 ,  C12N9/00 ,  A01H1/00

CPC分类号： C12N9/001 ,  C12N9/1085 ,  C12N9/88 ,  C12N9/90 ,  C12N15/8251 ,  C12N15/8253 ,  C12N15/8254

摘要： This invention relates to an isolated nucleic acid fragment encoding a plant enzyme that catalyze steps in the biosynthesis of lysine, threonine, methionine, cysteine and isoleucine from aspartate, the enzyme a member selected from the group consisting of: dihydrodipicolinate reductase, diaminopimelate epimerase, threonine synthase, threonine deaminase and S-adenosylmethionine synthetase. The invention also relates to the construction of a chimeric gene encoding all or a portion of the enzyme, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the enzyme in a transformed host cell.

摘要翻译： 本发明涉及编码植物酶的分离的核酸片段，其催化从天冬氨酸生物合成赖氨酸，苏氨酸，甲硫氨酸，半胱氨酸和异亮氨酸的步骤，所述酶选自二氢吡啶二羧酸还原酶，二氨基庚二酸差向异构酶，苏氨酸 合酶，苏氨酸脱氨酶和S-腺苷甲硫氨酸合成酶。 本发明还涉及在正义或反义方向上构建编码全部或部分酶的嵌合基因，其中嵌合基因的表达导致转化的宿主细胞中酶的水平发生变化。

公开(公告)号：US07291765B2

公开(公告)日：2007-11-06

申请号：US11622573

申请日：2007-01-12

申请人： Zhan-Bin Liu ,  Johan M. Stoop

发明人： Zhan-Bin Liu ,  Johan M. Stoop

IPC分类号： C12N15/82 ,  C12N5/04 ,  C12N5/10 ,  A01H5/00 ,  A01H5/10

CPC分类号： C12N15/8234 ,  C12N15/8245

摘要： The promoters of a soybean seed maturation protein PM29 and a soybean late-embryogenesis abundant protein LEA3 and fragments thereof and their use in promoting the expression of one or more heterologous nucleic acid fragments in plants are described.

摘要翻译： 描述了大豆种子成熟蛋白PM29和大豆晚胚发生丰富蛋白LEA3及其片段的启动子及其在促进植物中一种或多种异源核酸片段表达的用途。

公开(公告)号：US20050120405A1

公开(公告)日：2005-06-02

申请号：US10917602

申请日：2004-08-13

申请人： Saverio Falco ,  Zhan-Bin Liu

发明人： Saverio Falco ,  Zhan-Bin Liu

IPC分类号： C12N9/02 ,  C12N9/10 ,  C12N9/88 ,  C12N9/90 ,  C12N15/82 ,  A01H1/00 ,  C12Q1/68

CPC分类号： C12N9/001 ,  C12N9/1085 ,  C12N9/88 ,  C12N9/90 ,  C12N15/8251 ,  C12N15/8253 ,  C12N15/8254

摘要： This invention relates to an isolated nucleic acid fragment encoding a plant enzyme that catalyze steps in the biosynthesis of lysine, threonine, methionine, cysteine and isoleucine from aspartate, the enzyme a member selected from the group consisting of: dihydrodipicolinate reductase, diaminopimelate epimerase, threonine synthase, threonine deaminase and S-adenosylmethionine synthetase. The invention also relates to the construction of a chimeric gene encoding all or a portion of the enzyme, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the enzyme in a transformed host cell

摘要翻译： 本发明涉及编码植物酶的分离的核酸片段，其催化从天冬氨酸生物合成赖氨酸，苏氨酸，甲硫氨酸，半胱氨酸和异亮氨酸的步骤，所述酶选自二氢吡啶二羧酸还原酶，二氨基庚二酸差向异构酶，苏氨酸 合酶，苏氨酸脱氨酶和S-腺苷甲硫氨酸合成酶。 本发明还涉及在正义或反义方向上构建编码全部或部分酶的嵌合基因，其中嵌合基因的表达导致转化的宿主细胞中酶的水平的改变