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    Glutathione-detecting fluorescent probe
    11.
    发明授权
    Glutathione-detecting fluorescent probe 有权

    公开(公告)号：US10261090B2

    公开(公告)日：2019-04-16

    申请号：US15116661

    申请日：2015-02-24

    Applicant: THE UNIVERSITY OF TOKYO

    Inventor： Yasuteru Urano ,  Mako Kamiya ,  Keitaro Umezawa ,  Masafumi Yoshida

    IPC: G01N33/68 ,  C09B11/08 ,  C09B11/24 ,  C09B11/28 ,  C09B19/00 ,  C09K11/07 ,  G01N21/64 ,  C07F7/08 ,  G01N33/58 ,  C09K11/06

    Abstract: [Problem] To provide the following: a novel fluorescent probe for detecting a compound, such as glutathione, that contains a —SH group; a detection method using said fluorescent probe; and a detection kit containing said probe.[Solution] A fluorescent probe for detecting a compound containing an —SH group, wherein the fluorescent probe comprises a compound represented by formula (I) or a salt thereof (In the formula, X represents Si(Ra)(Rb), Ge(Ra)(Rb), Sn(Ra)(Rb), C(Ra)(Rb), or O (wherein Ra and Rb each independently represent a hydrogen atom or alkyl group); R1 represents a hydrogen atom, or 1-4 identical or different substituents independently selected from the group consisting of a cyano group, alkyl group, carboxyl group, ester group, alkoxy group, amide group, and azide group, each of which may be optionally substituted; R2 represents a hydrogen atom, halogen atom, hydroxyl group, cyano group, or an alkyl group, alkynyl group, alkoxy group, aryl, or heteroaryl, each of which may be optionally substituted; R3 and R4 each independently represent a hydrogen atom or 1-3 identical or different substituents independently selected from the group consisting of a hydroxyl group, halogen atom, or an alkyl group, sulfo group, carboxyl group, ester group, amide group and azide group, each of which may be optionally substituted; R5, R6, R7 and R8 each independently represent a hydrogen atom or alkyl group, wherein, R5 or R6, respectively together with R3, may form a ring structure including the nitrogen atoms bonded thereto, or R7 or R8, respectively together with R4, may form a ring structure including the nitrogen atoms bonded thereto).

    FLUORESCENT PROBE FOR HIGH-SENSITIVITY PANCREATIC FLUID DETECTION, AND METHOD FOR DETECTING PANCREATIC FLUID
    13.
    发明申请
    FLUORESCENT PROBE FOR HIGH-SENSITIVITY PANCREATIC FLUID DETECTION, AND METHOD FOR DETECTING PANCREATIC FLUID 有权
    用于高灵敏度胰酶检测的荧光探针和用于检测胰腺液的方法

    公开(公告)号：US20150152469A1

    公开(公告)日：2015-06-04

    申请号：US14398202

    申请日：2013-05-29

    Applicant: THE UNIVERSITY OF TOKYO

    Inventor： Yasuteru Urano ,  Masayo Sakabe ,  Tetsuo Nagano ,  Takeaki Ishizawa ,  Suguru Yamashita ,  Norihiro Kokudo ,  Mako Kamiya

    IPC: C12Q1/37 ,  C12Q1/48

    CPC classification number: C12Q1/37 ,  A61K49/0041 ,  A61K49/006 ,  C07D493/10 ,  C09B11/24 ,  C09K11/06 ,  C09K2211/1007 ,  C09K2211/1011 ,  C09K2211/1088 ,  C12Q1/48

    Abstract: [Problem]To provide a fluorescent probe capable of rapidly detecting and imaging with high sensitivity the presence of leakage of pancreatic fluid during or after surgery, and to furthermore provide a detection method and detection kit that use the fluorescent probe.[Solution]A fluorescent probe for detecting pancreatic fluid containing a compound or salt thereof represented by formula (I) below. (In the formula, A represents an amino acid residue or N-substituted amino acid residue, with A forming an amide bond with the adjacent NH in the formula; R1 represents a hydrogen atom or 1 to 4 same or different substituent groups that bond with a benzene ring; R2, R3, R4, R5, R6, and R7 each independently represent a hydrogen atom, a hydroxyl group, an alkyl group, or a halogen atom; R8 and R9 each independently represent a hydrogen atom or an alkyl group; and X represents a C1-C3 alkylene group.)

    Abstract translation: [问题]提供能够以高灵敏度快速检测和成像能够在手术期间或手术后存在胰液泄漏的荧光探针，并且还提供使用荧光探针的检测方法和检测试剂盒。 [解决方案]一种用于检测含有下式（I）表示的化合物或其盐的胰液的荧光探针。 （式中，A表示氨基酸残基或N-取代氨基酸残基，A与式中相邻的NH形成酰胺键; R1表示氢原子或1〜4个相同或不同的与 苯环; R2，R3，R4，R5，R6和R7各自独立地表示氢原子，羟基，烷基或卤素原子; R8和R9各自独立地表示氢原子或烷基; X表示C1-C3亚烷基。）

    Method for Detecting Enzyme Activity and Fluorescent Probe Used in Said Method
    14.
    发明申请
    Method for Detecting Enzyme Activity and Fluorescent Probe Used in Said Method 有权

    公开(公告)号：US20250093270A1

    公开(公告)日：2025-03-20

    申请号：US18843204

    申请日：2023-03-02

    Applicant: The University of Tokyo ,  RIKEN

    Inventor： Yasuteru Urano ,  Toru Komatsu ,  Shingo Sakamoto ,  Tatsuya Ukegawa ,  Rikiya Watanabe

    IPC: G01N21/77 ,  C07F5/02 ,  C07F7/08 ,  C12Q1/34 ,  C12Q1/46

    Abstract: An object of the present invention is to provide a method capable of detecting activity of an enzyme that strictly recognizes a substrate, such as acetylcholinesterase (AChE). There is provided a method for detecting activity of an enzyme in a biological sample using a microdevice, the method including a step of bringing the biological sample into contact with a substrate analog or a natural substrate, and a compound represented by General Formula (I) below or a salt thereof, wherein the substrate analog or the natural substrate generates a compound A having a thiol group (R—SH), a selenol group (R—SeH), or a poly sulfur atom (R—(S)n-H) (R is a hydrogen atom or an alkyl group, and n is 1 to 5) by a reaction with the enzyme, and fluorescence is observed or measured, the fluorescence being emitted or enhanced from a fluorescent product generated by the reaction of the compound represented by General Formula (I) or the salt thereof with the thiol group, the selenol group, or the poly sulfur atom.

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