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公开(公告)号：US09670516B2
公开(公告)日：2017-06-06
申请号：US14406841
申请日：2013-06-11
IPC分类号： C12P1/00 , C12P19/14 , C12P7/10 , C12P7/56 , C13K1/02 , C12P19/02 , C13K1/04 , C12P7/14 , C13K13/00
CPC分类号： C12P19/14 , C12P7/10 , C12P7/14 , C12P7/56 , C12P19/02 , C12P2201/00 , C13K1/02 , C13K1/04 , C13K13/002 , Y02E50/16
摘要： A method of producing a sugar liquid from cellulose-containing biomass includes (1) to (4): (1) subjecting a cellulose-containing biomass to a dilute sulfuric acid treatment and thereafter separating the treated cellulose-containing biomass into a dilute sulfuric acid-treated liquid and a cellulose-containing solid content; (2) adding a cellulase to the cellulose-containing solid content to hydrolyze the cellulose and thereafter obtaining a sugar liquid; (3) filtering the dilute sulfuric acid-treated liquid through a nanofiltration membrane at pH 2.5 or lower to thereby separate a sugar concentrated liquid as a retentate and at the same time recover a sulfuric acid aqueous solution as a permeate; and (4) reusing the whole amount or a part of the sulfuric acid aqueous solution obtained in (3) in the dilute sulfuric acid treatment in (1).
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公开(公告)号：US20150140606A1
公开(公告)日：2015-05-21
申请号：US14406841
申请日：2013-06-11
CPC分类号： C12P19/14 , C12P7/10 , C12P7/14 , C12P7/56 , C12P19/02 , C12P2201/00 , C13K1/02 , C13K1/04 , C13K13/002 , Y02E50/16
摘要： A method of producing a sugar liquid from cellulose-containing biomass includes (1) to (4): (1) subjecting a cellulose-containing biomass to a dilute sulfuric acid treatment and thereafter separating the treated cellulose-containing biomass into a dilute sulfuric acid-treated liquid and a cellulose-containing solid content; (2) adding a cellulase to the cellulose-containing solid content to hydrolyze the cellulose and thereafter obtaining a sugar liquid; (3) filtering the dilute sulfuric acid-treated liquid through a nanofiltration membrane at pH 2.5 or lower to thereby separate a sugar concentrated liquid as a retentate and at the same time recover a sulfuric acid aqueous solution as a permeate; and (4) reusing the whole amount or a part of the sulfuric acid aqueous solution obtained in (3) in the dilute sulfuric acid treatment in (1).
摘要翻译： 从含纤维素生物质制备糖液的方法包括（1）至（4）：（1）对含纤维素的生物质进行稀硫酸处理，然后将经处理的含纤维素的生物质分离成稀硫酸 处理的液体和含纤维素的固体含量; （2）将纤维素酶加入到含纤维素的固体成分中以水解纤维素，然后得到糖液; （3）通过pH2.5以下的纳滤膜对稀硫酸处理液进行过滤，分离作为滞留物的糖浓缩液，同时回收作为渗透液的硫酸水溶液; （4）将（3）中得到的全部或一部分硫酸水溶液重新用于（1）中的稀硫酸处理。
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公开(公告)号：US20140295525A1
公开(公告)日：2014-10-02
申请号：US14359122
申请日：2012-11-21
CPC分类号： B01D61/18 , B01D61/145 , B01D61/16 , B01D2311/2688 , B01D2317/025 , B01D2317/027 , C12M21/14 , C12M47/10 , C12N9/2437 , C12P21/00
摘要： A method of producing cellulase includes steps (1) to (3): (1) subjecting an aqueous solution of cellulase derived from filamentaous fungi to filtration through an ultrafiltration membrane with a molecular weight cut off of 100,000 to 200,000 to obtain a filtrate and concurrently obtain a concentrated enzyme liquid as a retentate; (2) further subjecting the filtrate obtained in step (1) to filtration through a second ultrafiltration membrane with a molecular weight cut off of 5,000 to 50,000 to obtain a second concentrated enzyme liquid as a retentate; and (3) mixing the concentrated enzyme liquid obtained in steps (1) and (2) to obtain cellulase derived from filamentaous fungi.
摘要翻译： 纤维素酶的制造方法包括以下工序（1）〜（3）：（1）将来自丝状真菌的纤维素酶水溶液通过分子量为100,000〜200,000的超滤膜过滤，得到滤液 获得浓缩的酶液作为保留物; （2）进一步使步骤（1）中得到的滤液通过分子量为5000〜50,000的第二超滤膜过滤，得到第二浓缩酶液体作为滞留物; 和（3）将步骤（1）和（2）中获得的浓缩酶液体混合以获得源自丝状真菌的纤维素酶。
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14.发明申请METHOD FOR PRODUCING GLUCOSIDASE, ENZYME COMPOSITION, AND METHOD FOR HYDROLYZING BIOMASS 有权生产葡萄球菌素的方法，酶组合物和水解生物量的方法公开(公告)号：US20130189761A1
公开(公告)日：2013-07-25
申请号：US13772533
申请日：2013-02-21
发明人： Hiroyuki Kurihara , Shiomi Watanabe , Katsushige Yamada , Kazuhiko Ishikawa , Yasunobu Wada , Yuji Kado
IPC分类号： C12N9/42
CPC分类号： C12N9/2437 , C12N9/2445 , C12P21/005 , C12Y302/01021
摘要： A method for producing a mutant glucosidase includes introducing DNA encoding a secretion signal sequence and DNA encoding Asn-X-Ser or Asn-X-Thr into DNA encoding a glucosidase derived from a thermophile, and introducing the resulting DNA into a eukaryotic microorganism and expressing it as a secretory protein. An enzyme composition contains the mutant glucosidase.
摘要翻译： 制备突变型葡糖苷酶的方法包括将编码分泌信号序列的DNA和编码Asn-X-Ser或Asn-X-Thr的DNA引入编码嗜热菌的葡糖苷酶的DNA中，并将得到的DNA导入真核生物微生物并表达 它作为分泌蛋白。 酶组合物含有突变型葡糖苷酶。
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公开(公告)号：US11413582B2
公开(公告)日：2022-08-16
申请号：US16763288
申请日：2018-11-29
摘要： A filtration device includes a continuous first unit including a first membrane that separates a liquid into first permeated and non-permeated liquids, a first adjuster that adjusts a flow rate of the first permeated liquid to be substantially constant, and a first liquid scale that detects a liquid amount, a second unit including a second membrane that separates another liquid into second permeated and non-permeated liquids, a second adjuster that adjusts a flow rate of the second permeated liquid to be substantially constant, and a second liquid scale that detects another liquid amount, a first controller that controls the liquid amount in the first storage tank based on measurement values from continuous two first units or from the continuous first and second units, and a second controller that controls the another liquid amount based on a measurement value from the second unit.
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公开(公告)号：US11253818B2
公开(公告)日：2022-02-22
申请号：US14359122
申请日：2012-11-21
摘要： A method of producing cellulase includes steps (1) to (3): (1) subjecting an aqueous solution of cellulase derived from filamentous fungi to filtration through an ultrafiltration membrane with a molecular weight cut off of 100,000 to 200,000 to obtain a filtrate and concurrently obtain a concentrated enzyme liquid as a retentate; (2) further subjecting the filtrate obtained in step (1) to filtration through a second ultrafiltration membrane with a molecular weight cut off of 5,000 to 50,000 to obtain a second concentrated enzyme liquid as a retentate; and (3) mixing the concentrated enzyme liquid obtained in steps (1) and (2) to obtain cellulase derived from filamentous fungi.
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公开(公告)号：US11091698B2
公开(公告)日：2021-08-17
申请号：US16648747
申请日：2018-09-25
摘要： An anti-caking agent for soil includes, as effective ingredients: a lignin having a molecular weight peak, as measured by GPC molecular weight analysis using a UV detector at a wavelength of 254 nm, within the molecular weight range of 10,000 to 40,000; and a diatomaceous earth. A method of improving the growth of a plant uses the above described anti-caking agent for soil. The anti-caking agent for soil has the effect of reducing the caking of soil and improving the poor growth of a plant.
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公开(公告)号：US10815501B2
公开(公告)日：2020-10-27
申请号：US15954752
申请日：2018-04-17
IPC分类号： C12P7/14 , C12P19/02 , C12P19/14 , C12N1/22 , C12P7/10 , C13B20/00 , C13B30/00 , C13B50/00 , C13K1/04 , C13K5/00 , C13K13/00
摘要： A sugar liquid or solid sugar is derived from cellulose-containing biomass or blackstrap molasses, wherein content of one or more free amino acids selected from the group consisting of serine, threonine, asparagine, aspartic acid, glutamine, glutamic acid, proline, phenyl alanine, lysine, and histidine, which are impurities, is below the limit of detection.
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公开(公告)号：US20200283682A1
公开(公告)日：2020-09-10
申请号：US16648747
申请日：2018-09-25
摘要： An anti-caking agent for soil includes, as effective ingredients: a lignin having a molecular weight peak, as measured by GPC molecular weight analysis using a UV detector at a wavelength of 254 nm, within the molecular weight range of 10,000 to 40,000; and a diatomaceous earth. A method of improving the growth of a plant uses the above described anti-caking agent for soil. The anti-caking agent for soil has the effect of reducing the caking of soil and improving the poor growth of a plant.
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公开(公告)号：US20190112624A1
公开(公告)日：2019-04-18
申请号：US16089918
申请日：2017-03-28
发明人： Atsushi Minamino , Shigeyuki Funada , Hiroyuki Kurihara , Yuka Asahi , Takuya Kasahara , Katsushige Yamada
摘要： A method efficiently produces a sugar liquid having a high quality from a cellulose-containing biomass. More specifically, the method includes: the step of obtaining an alkaline filtrate by allowing an alkaline aqueous medium to pass through a cellulose-containing biomass; the recirculation filtration step of allowing the alkaline filtrate to repeatedly pass through the cellulose-containing biomass to produce a cellulose-containing solid component; and the step of hydrolyzing the cellulose-containing solid component to obtain the sugar liquid.
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