公开(公告)号：US06569658B1

公开(公告)日：2003-05-27

申请号：US09645791

申请日：2000-08-24

Applicant: Ying Luo ,  C. Alan Fu ,  Mary Shen

Inventor： Ying Luo ,  C. Alan Fu ,  Mary Shen

IPC: C12N912

CPC classification number: C07K14/4738

Abstract: The present invention is directed to novel polypeptides, nucleic acids and related molecules which have an effect on or are related to the cell cycle. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides of the present invention and to methods for producing the polypeptides of the present invention. Further provided by the present invention are methods for identifying novel compositions which mediate cell cycle bioactivity, and the use of such compositions in diagnosis and treatment of disease.

Abstract translation: 本发明涉及对细胞周期有影响或与细胞周期有关的新型多肽，核酸和相关分子。 本文还提供了包含那些核酸序列的载体和宿主细胞，包含与异源多肽序列融合的本发明的多肽的嵌合多肽分子，与本发明的多肽结合的抗体以及本发明的多肽的制备方法 发明。 本发明还提供了鉴定介导细胞周期生物活性的新型组合物的方法，以及这些组合物在疾病诊断和治疗中的应用。

公开(公告)号：US06562591B1

公开(公告)日：2003-05-13

申请号：US09425324

申请日：1999-10-21

Applicant: Ying Luo ,  C. Alan Fu ,  Mary Shen

Inventor： Ying Luo ,  C. Alan Fu ,  Mary Shen

IPC: C12P2106

CPC classification number: C07K14/4738

Abstract: The present invention is directed to polypeptides, nucleic acids and related molecules which have an effect on or are related to the cell cycle. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides of the present invention and to methods for producing the polypeptides of the present invention. Further provided by the present invention are methods for identifying novel compositions which mediate cell cycle bioactivity, and the use of such compositions in diagnosis and treatment of disease.

Abstract translation: 本发明涉及对细胞周期有影响或与细胞周期有关的多肽，核酸和相关分子。 本文还提供了包含那些核酸序列的载体和宿主细胞，包含与异源多肽序列融合的本发明的多肽的嵌合多肽分子，与本发明的多肽结合的抗体以及本发明的多肽的制备方法 发明。 本发明还提供了鉴定介导细胞周期生物活性的新型组合物的方法，以及这些组合物在疾病诊断和治疗中的应用。

公开(公告)号：US07863222B2

公开(公告)日：2011-01-04

申请号：US12221325

申请日：2008-08-01

Applicant: Donald G. Payan ,  Mary Shen ,  Simon Yu ,  Todd M. Kinsella ,  Yasumichi Hitoshi

Inventor： Donald G. Payan ,  Mary Shen ,  Simon Yu ,  Todd M. Kinsella ,  Yasumichi Hitoshi

IPC: C40B50/06 ,  C40B40/08 ,  C40B30/06 ,  C40B40/02 ,  C12P19/34 ,  A61K49/12

CPC classification number: C12N15/1093 ,  C12N15/66 ,  C12P19/34 ,  C40B40/08 ,  C40B50/06

Abstract: As noted above, certain aspects of this disclosure relate to a library of nucleic acid vectors, as well as a method for making the same. In certain embodiments, the library of nucleic acid vectors comprises: a plurality of nucleic acid molecules of the following formula: S1—R—S2 wherein, in each nucleic acid of the plurality: S1 and S2 are each at least 15 nucleotides in length; S1 and S2 are complementary to each other along their entire length; either S1 or S2 is complementary along its entire length to a sequence in eukaryotic mRNA; and R is a six base recognition site for a restriction endonuclease; and wherein S1 and S2 vary in nucleotide sequence between different members of the plurality. A method for amplifying a circular nucleic acid is also provided.

Abstract translation: 如上所述，本公开的某些方面涉及核酸载体文库以及其制备方法。 在某些实施方案中，核酸载体文库包含：多个下式的核酸分子：S1-R-S2，其中在多个的每个核酸中，S1和S2的长度各自为至少15个核苷酸; S1和S2沿其整个长度彼此互补; S1或S2在其整个长度上与真核mRNA中的序列互补; R是限制性内切核酸酶的六碱基识别位点; 并且其中S1和S2在多个不同成员之间的核苷酸序列变化。 还提供了扩增环状核酸的方法。

公开(公告)号：US06808906B2

公开(公告)日：2004-10-26

申请号：US10142662

申请日：2002-05-08

Applicant: Mary Shen ,  Simon Yu ,  Xian Wu ,  Donald Payan

Inventor： Mary Shen ,  Simon Yu ,  Xian Wu ,  Donald Payan

IPC: C12N1566

CPC classification number: C12N15/86 ,  C12N15/66 ,  C12N2740/13043 ,  C12N2800/70 ,  C12Q1/6855 ,  C12Q2525/185

Abstract: The present invention provides random cDNA expression vector libraries, comprising expression vectors which comprise random cDNAs positioned in sense orientation. Also provided are random cDNA expression vector libraries, comprising expression vectors which comprise random cDNAs positioned in antisense orientation. Methods for producing these libraries through directional cloning of random cDNAs are also provided. Also provided herein are methods of using these libraries to screen for agents capable of modulating cell phenotype in desirable ways.

Abstract translation: 本发明提供随机cDNA表达载体文库，其包含表达载体，其包含位于正义取向的随机cDNA。 还提供了随机cDNA表达载体文库，其包含表达载体，其包含以反义方向定位的随机cDNA。 还提供了通过定向克隆随机cDNA产生这些文库的方法。 本文还提供了使用这些文库筛选能够以期望的方式调节细胞表型的试剂的方法。

公开(公告)号：US20100029505A1

公开(公告)日：2010-02-04

申请号：US12221325

申请日：2008-08-01

Applicant: Donald G. Payan ,  Mary Shen ,  Simon Yu ,  Todd M. Kinsella ,  Yasumichi Hitoshi

Inventor： Donald G. Payan ,  Mary Shen ,  Simon Yu ,  Todd M. Kinsella ,  Yasumichi Hitoshi

IPC: C40B30/06 ,  C40B40/08 ,  C40B50/06 ,  C12P19/34 ,  C40B40/02

CPC classification number: C12N15/1093 ,  C12N15/66 ,  C12P19/34 ,  C40B40/08 ,  C40B50/06

Abstract: As noted above, certain aspects of this disclosure relate to a library of nucleic acid vectors, as well as a method for making the same. In certain embodiments, the library of nucleic acid vectors comprises: a plurality of nucleic acid molecules of the following formula: S1—R—S2 wherein, in each nucleic acid of the plurality: S1 and S2 are each at least 15 nucleotides in length; S1 and S2 are complementary to each other along their entire length; either S1 or S2 is complementary along its entire length to a sequence in eukaryotic mRNA; and R is a six base recognition site for a restriction endonuclease; and wherein S1 and S2 vary in nucleotide sequence between different members of the plurality. A method for amplifying a circular nucleic acid is also provided.

Abstract translation: 如上所述，本公开的某些方面涉及核酸载体文库以及其制备方法。 在某些实施方案中，核酸载体文库包含：多个下式的核酸分子：S1-R-S2，其中在多个的每个核酸中，S1和S2的长度各自为至少15个核苷酸; S1和S2沿其整个长度彼此互补; S1或S2在其整个长度上与真核mRNA中的序列互补; R是限制性内切核酸酶的六碱基识别位点; 并且其中S1和S2在多个不同成员之间的核苷酸序列变化。 还提供了扩增环状核酸的方法。

公开(公告)号：US07034145B2

公开(公告)日：2006-04-25

申请号：US10933855

申请日：2004-09-03

Applicant: Mary Shen ,  Simon Yu ,  Xian Wu ,  Donald Payan

Inventor： Mary Shen ,  Simon Yu ,  Xian Wu ,  Donald Payan

IPC: C07H21/04

CPC classification number: C12N15/86 ,  C12N15/66 ,  C12N2740/13043 ,  C12N2800/70 ,  C12Q1/6855 ,  C12Q2525/185

Abstract: The present invention provides random cDNA expression vector libraries, comprising expression vectors which comprise random cDNAs positioned in sense orientation. Also provided are random cDNA expression vector libraries, comprising expression vectors which comprise random cDNAs positioned in antisense orientation. Methods for producing these libraries through directional cloning of random cDNAs are also provided. Also provided herein are methods of using these libraries to screen for agents capable of modulating cell phenotype in desirable ways.

公开(公告)号：US20050032110A1

公开(公告)日：2005-02-10

申请号：US10933855

申请日：2004-09-03

Applicant: Mary Shen ,  Simon Yu ,  Xian Wu ,  Donald Payan

Inventor： Mary Shen ,  Simon Yu ,  Xian Wu ,  Donald Payan

IPC: C12N15/66 ,  C12N15/867 ,  C12Q1/68

CPC classification number: C12N15/86 ,  C12N15/66 ,  C12N2740/13043 ,  C12N2800/70 ,  C12Q1/6855 ,  C12Q2525/185

Abstract: The present invention provides random cDNA expression vector libraries, comprising expression vectors which comprise random cDNAs positioned in sense orientation. Also provided are random cDNA expression vector libraries, comprising expression vectors which comprise random cDNAs positioned in antisense orientation. Methods for producing these libraries through directional cloning of random cDNAs are also provided. Also provided herein are methods of using these libraries to screen for agents capable of modulating cell phenotype in desirable ways.

Abstract translation: 本发明提供随机cDNA表达载体文库，其包含表达载体，其包含位于正义取向的随机cDNA。 还提供了随机cDNA表达载体文库，其包含表达载体，其包含以反义方向定位的随机cDNA。 还提供了通过定向克隆随机cDNA产生这些文库的方法。 本文还提供了使用这些文库筛选能够以期望的方式调节细胞表型的试剂的方法。