公开(公告)号：US08409795B2

公开(公告)日：2013-04-02

申请号：US12175388

申请日：2008-07-17

申请人： Daniel J. Schneider ,  Sheri K. Wilcox ,  Dominic Zichi ,  Dan Nieuwlandt ,  Jeff Carter ,  Larry Gold

发明人： Daniel J. Schneider ,  Sheri K. Wilcox ,  Dominic Zichi ,  Dan Nieuwlandt ,  Jeff Carter ,  Larry Gold

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6811 ,  C12N15/1048 ,  C12N15/111 ,  C12N15/115 ,  C12N2310/16 ,  C12N2320/13 ,  C12Q2541/101 ,  C12Q2525/205 ,  C12Q2525/117

摘要： The present disclosure describes improved SELEX methods for generating nucleic acid ligands that are capable of binding to target molecules and improved photoSELEX methods for generating photoreactive nucleic acid ligands that are capable of both binding and covalently crosslinking to target molecules. The disclosure further describes nucleic acid libraries having expanded physical and chemical properties and their use in SELEX and photoSELEX; methods for increasing the crosslinking efficiencies of photoaptamers; methods for producing photoaptamers having selective modifications that enhance functionality and minimize non-specific photoreactions; and methods for generating truncated nucleic acid ligands from nucleic acid ligands of longer length. The disclosure further describes aptamers and photoaptamers obtained by using any of the foregoing.

摘要翻译： 本公开描述了用于产生能够结合靶分子的核酸配体的改进的SELEX方法和用于产生能够与靶分子结合和共价交联的光反应性核酸配体的改进的photoSELEX方法。 本公开进一步描述了具有扩展的物理和化学性质及其在SELEX和photoSELEX中的用途的核酸文库; 提高光催化剂交联效率的方法; 制备具有增强功能并使非特异性光反应最小化的选择性修饰的光致抗体的方法; 以及用于从较长长度的核酸配体产生截短的核酸配体的方法。 本公开进一步描述了通过使用上述任何一种获得的适体和光致抗体。

公开(公告)号：US20120143805A1

公开(公告)日：2012-06-07

申请号：US13390648

申请日：2010-03-05

申请人： Larry Gold ,  Edward N. Brody ,  Rachel M. Ostroff ,  Dominic Zichi ,  Alex A.E. Stewart ,  Michael Riel-Mehan ,  Mark Messenbaugh ,  Randee S. Schwartz ,  Jeffery Walker ,  Stephen Alaric Williams ,  Malti Nikrad

发明人： Larry Gold ,  Edward N. Brody ,  Rachel M. Ostroff ,  Dominic Zichi ,  Alex A.E. Stewart ,  Michael Riel-Mehan ,  Mark Messenbaugh ,  Randee S. Schwartz ,  Jeffery Walker ,  Stephen Alaric Williams ,  Malti Nikrad

IPC分类号： G06N5/00 ,  C40B30/04

CPC分类号： G01N33/57423 ,  G01N33/574 ,  G01N2800/60 ,  G16H50/20

摘要： The present disclosure includes biomarkers, methods, devices, reagents, systems, and kits for the detection and diagnosis of cancer. In one aspect, the disclosure provides biomarkers that can be used alone or in various combinations to diagnose cancer. In another aspect, methods are provided for diagnosing cancer in an individual, where the methods include detecting, in a biological sample from an individual, at least one biomarker value corresponding to at least one biomarker selected from the group of biomarkers provided in Table 47, wherein the individual is classified as having cancer, or the likelihood of the individual having cancer is determined, based on the at least one biomarker value.

摘要翻译： 本公开包括用于癌症的检测和诊断的生物标志物，方法，装置，试剂，系统和试剂盒。 一方面，本公开提供可以单独使用或以各种组合使用以诊断癌症的生物标志物。 在另一方面，提供了用于诊断个体癌症的方法，其中所述方法包括在来自个体的生物样品中检测至少一个对应于选自表47中提供的生物标志物组的生物标志物的生物标记值， 其中所述个体基于所述至少一种生物标志物值被分类为具有癌症或确定个体患有癌症的可能性。

公开(公告)号：US07964356B2

公开(公告)日：2011-06-21

申请号：US12499967

申请日：2009-07-09

申请人： Dominic Zichi ,  Sheri K. Wilcox ,  Chris Bock ,  Daniel J. Schneider ,  Bruce Eaton ,  Larry Gold

发明人： Dominic Zichi ,  Sheri K. Wilcox ,  Chris Bock ,  Daniel J. Schneider ,  Bruce Eaton ,  Larry Gold

IPC分类号： C12Q1/68 ,  C07H21/02

CPC分类号： C12N15/1048 ,  C12Q1/6811 ,  C12Q2525/205

摘要： The present disclosure describes methods for producing aptamers and photoaptamers having slower dissociation rate constants than are obtained using prior SELEX and photoSELEX methods. The disclosure further describes aptamers and photoaptamers having slower dissociation rate constants than those obtained using prior methods. This invention relates to the field of diagnostic histology, cytology, histopathology, and cytopathology methods and reagents for the detection of various disease states. More specifically, the invention relates to the use of aptamers in histologic, cytologic, histopathic, and/or cytopathic diagnostic methods. Aptamers may be provided that react with specific target molecules contained within a histological or cytological sample. Aptamers may be used to assess localization, relative density, and presence or absence of one or more target. Targets may be selected that are specific and diagnostic of a given disease state for which the sample was collected. Aptamers may be used to introduce target specific signal moieties. Antigen retrieval methods may be applied to the sample prior to reaction with the specific aptamer/s to improve interaction of the aptamer and target within the sample. Or aptamers may be developed for the specific target that eliminates the need for the antigen retrieval process. In addition to target identification, aptamers may be used to amplify signal generation through a variety of methods.

摘要翻译： 本公开描述了用于产生具有比使用先前的SELEX和photoSELEX方法获得的解离速率常数更慢的适体和光催化剂的方法。 本公开进一步描述了具有比使用现有方法获得的那些更快的解离速率常数的适体和光致抗体。 本发明涉及用于检测各种疾病状态的诊断组织学，细胞学，组织病理学和细胞病理学方法和试剂领域。 更具体地，本发明涉及适配体在组织学，细胞学，组织病理学和/或细胞病变诊断方法中的用途。 可以提供与包含在组织学或细胞学样品中的特定靶分子反应的适配子。 可以使用适配器来评估定位，相对密度以及一个或多个靶的存在或不存在。 可以选择对于样品收集的给定疾病状态的特异性和诊断性的靶标。 适配子可用于引入目标特异性信号部分。 抗原检索方法可以在与特异性适体反应之前应用于样品，以改善样品中适体和靶标的相互作用。 或者可以针对特定靶标开发适配体，从而消除对抗原检索过程的需要。 除目标识别之外，适体可用于通过各种方法放大信号产生。

公开(公告)号：US07947447B2

公开(公告)日：2011-05-24

申请号：US12175434

申请日：2008-07-17

申请人： Dominic Zichi ,  Sheri K. Wilcox ,  Chris Bock ,  Daniel J. Schneider ,  Bruce Eaton ,  Larry Gold

发明人： Dominic Zichi ,  Sheri K. Wilcox ,  Chris Bock ,  Daniel J. Schneider ,  Bruce Eaton ,  Larry Gold

IPC分类号： C12Q1/68 ,  C07H21/02

CPC分类号： C12N15/115 ,  C12N9/6429 ,  C12N15/1048 ,  C12N2310/16 ,  C12N2310/314 ,  C12N2310/315 ,  C12N2310/321 ,  C12N2310/322 ,  C12N2310/333 ,  C12N2310/334 ,  C12N2310/335 ,  C12N2310/336 ,  C12N2330/31 ,  C12Q1/6811 ,  C12Q1/6816 ,  C12Q1/6832 ,  C12Q1/6834 ,  G01N33/5308 ,  G01N33/58 ,  C12Q2525/205 ,  C12Q2565/514 ,  C12Q2525/161 ,  C12Q2537/101 ,  C12Q2523/313

摘要： The present disclosure describes improved SELEX methods for producing aptamers that are capable of binding to target molecules and improved photoSELEX methods for producing photoreactive aptamers that are capable of both binding and covalently crosslinking to target molecules. Specifically, the present disclosure describes methods for producing aptamers and photoaptamers having slower dissociation rate constants than are obtained using prior SELEX and photoSELEX methods. The disclosure further describes aptamers and photoaptamers having slower dissociation rate constants than those obtained using prior methods. In addition, the disclosure describes aptamer constructs that include a variety of functionalities, including a cleavable element, a detection element, and a capture or immobilization element.

摘要翻译： 本公开描述了用于产生能够结合靶分子的适配体的改进的SELEX方法，以及用于产生能够结合和共价交联靶分子的光反应性适体的改进的photoSELEX方法。 具体地，本公开描述了用于生产具有比使用先前的SELEX和photoSELEX方法获得的解离速率常数更慢的适体和光致抗体的方法。 本公开进一步描述了具有比使用现有方法获得的那些更快的解离速率常数的适体和光致抗体。 此外，本公开描述了包括多种功能的适体构建体，包括可切割元件，检测元件和捕获或固定元件。

公开(公告)号：US07629151B2

公开(公告)日：2009-12-08

申请号：US10717105

申请日：2003-11-18

申请人： Larry Gold ,  Dominic A. Zichi ,  Robert D. Jenison ,  Daniel J. Schneider

发明人： Larry Gold ,  Dominic A. Zichi ,  Robert D. Jenison ,  Daniel J. Schneider

IPC分类号： C12P19/34 ,  C12Q1/68 ,  C07H21/02 ,  C07H21/04 ,  C07H21/00

CPC分类号： C12N15/1048

摘要： The present invention includes a method and device for performing automated SELEX. The steps of the SELEX process are performed at one or more work stations on a work surface by a robotic manipulator controlled by a computer. The invention also includes methods and reagents to obviate the need for size-fractionation of amplified candidate nucleic acids before beginning the next round of the SELEX process.

公开(公告)号：US20230024434A1

公开(公告)日：2023-01-26

申请号：US17358186

申请日：2021-06-25

申请人： SomaLogic, Inc.

发明人： Larry Gold ,  Robert Kirk Delisle ,  David Sterling ,  Rachel Ostroff ,  Dom Zichi

IPC分类号： G01N33/68 ,  G01N33/50

摘要： The present invention relates to developing customized therapies for a disease or condition in a subject. In particular, the present invention relates to aptamer-based compositions and methods for identifying, modulating and monitoring drug targets in individual with a disease or condition, and further composition and methods for identifying and selecting protein targets for drug development.

公开(公告)号：US20210239692A1

公开(公告)日：2021-08-05

申请号：US17217006

申请日：2021-03-30

申请人： SomaLogic, Inc.

发明人： Glenn Sanders ,  Stephan Kraemer ,  Evaldas Katilius ,  EDGAR OLIVAS

IPC分类号： G01N33/543 ,  C12Q1/6811 ,  C07H21/00 ,  G01N33/53 ,  G01N33/68 ,  C12Q1/6804 ,  C12Q1/6837

摘要： The present disclosure describes methods, devices, reagents, and kits for the detection of one or more target molecules that may be present in a test sample. The described methods, devices, kits, and reagents facilitate the detection and quantification of a non-nucleic acid target (e.g., a protein target) in a test sample by detecting and quantifying a nucleic acid (i.e., an aptamer) where the aptamer-aptamer interactions are significantly reduced or eliminated while maintaining the aptamer-target interaction.

公开(公告)号：US20210215711A1

公开(公告)日：2021-07-15

申请号：US16074181

申请日：2017-02-07

申请人： SomaLogic, Inc.

发明人： Stuart Field

IPC分类号： G01N33/68

摘要： Methods, compositions, and kits for determining whether a subject has non-alcoholic fatty liver disease (NAFLD), and more specifically one or more of the following associated conditions of steatosis, lobular inflammation, hepatocyte ballooning and fibrosis, are provided. Methods, compositions, and kits for determining whether a subject has non-alcoholic steatosis are also provided. Methods, compositions, and kits for determining whether a subject has non-alcoholic steatohepatitis (NASH) are also provided.

公开(公告)号：US10808251B2

公开(公告)日：2020-10-20

申请号：US15525186

申请日：2015-11-23

申请人： SOMALOGIC, INC.

发明人： Urs Ochsner ,  Louis Green ,  Dom Zichi ,  Nebojsa Janjic

IPC分类号： C07H21/04 ,  C12N15/115 ,  G01N33/53 ,  C07K14/52

摘要： Described herein are aptamers capable of binding to growth differentiation factor 11 (GDF11) protein; compositions comprising a GDF11 binding aptamer with a GDF11 protein; and methods of making and using the same.

公开(公告)号：US10538771B2

公开(公告)日：2020-01-21

申请号：US16118596

申请日：2018-08-31

申请人： SomaLogic, Inc.

发明人： Urs A. Ochsner ,  Nebojsa Janjic

IPC分类号： C07H21/02 ,  C07H21/04 ,  C12N15/115 ,  G01N33/53 ,  G01N33/569 ,  C12N15/10 ,  C12Q1/14

摘要： Described herein are compositions and methods for detecting the presence or absence of a microorganism in a sample comprising contacting the sample with an aptamer capable of binding to a cell-surface protein of the microorganism to form a complex, contacting the mixture with a second aptamer capable of binding to the first cell-surface protein or a second cell-surface protein of the microorganism; and performing an assay to detect the second aptamer, wherein detecting the second aptamer indicates that the microorganism is present in the sample, and wherein not detecting the second aptamer indicates that the microorganism is absent from the sample.