公开(公告)号：US11788071B2

公开(公告)日：2023-10-17

申请号：US17223677

申请日：2021-04-06

Applicant: Codexis, Inc.

Inventor： Scott J. Novick ,  Nikki Dellas

IPC: C12N9/10

CPC classification number: C12N9/1096

Abstract: The present disclosure provides engineered transaminase polypeptides useful for the synthesis of chiral amine compounds under industrially relevant conditions. The disclosure also provides polynucleotides encoding the engineered transaminase polypeptides, host cells capable of expressing the engineered transaminases, and methods of using the engineered transaminases for the production of chiral amine compounds.

公开(公告)号：US20230313155A1

公开(公告)日：2023-10-05

申请号：US18164522

申请日：2023-02-03

Applicant: Codexis, Inc.

Inventor： Fabien Louis Cabirol ,  Haibin Chen ,  Anupam Gohel ,  Paulina Elena Salim ,  Derek J. Smith ,  Jacob Janey ,  Birgit Kosjek ,  Weng Lin Tang ,  Helen Hsieh ,  Son Q. Pham

IPC: C12N9/10 ,  C07D211/76 ,  C12P13/00 ,  C12P17/12

CPC classification number: C12N9/1096 ,  C07D211/76 ,  C12Y206/01 ,  C12P13/001 ,  C12P13/005 ,  C12P17/12

Abstract: The present disclosure relates to transaminase polypeptides capable of aminating a dicarbonyl substrate, and polynucleotides, vectors, host cells, and methods of making and using the transaminase polypeptides.

公开(公告)号：US20230265471A1

公开(公告)日：2023-08-24

申请号：US18004364

申请日：2021-06-28

Applicant: Evonik Operation GmbH

Inventor： Frank Schneider ,  Frank Jankowitsch

IPC: C12P13/00 ,  C12N9/10 ,  C12N15/52

CPC classification number: C12P13/00 ,  C12N9/1003 ,  C12N9/1022 ,  C12N9/1096 ,  C12N15/52 ,  C12Y201/04001 ,  C12Y203/03009 ,  C12Y206/01002 ,  C12Y206/01004 ,  C12Y206/01044

Abstract: A microorganism is transformed to be capable of producing guanidinoacetic acid (GAA). A method can be used for the fermentative production of GAA using such a microorganism. A corresponding method can be used for the fermentative production of creatine.

公开(公告)号：US20230227795A1

公开(公告)日：2023-07-20

申请号：US18004327

申请日：2021-06-28

Applicant: Evonik Operations GmbH

Inventor： Frank Schneider ,  Frank Jankowitsch

IPC: C12P13/04 ,  C12N9/10

CPC classification number: C12P13/04 ,  C12N9/1003 ,  C12Y201/04001 ,  C12N9/1096 ,  C12Y206/01002 ,  C12Y206/01004 ,  C12Y206/01044

Abstract: A microorganism is transformed to be capable of producing guanidinoacetic acid (GAA). A method can be used for the fermentative production of GAA using such a microorganism. A corresponding method can be used for the fermentative production of creatine.

公开(公告)号：US20230183706A1

公开(公告)日：2023-06-15

申请号：US17945151

申请日：2022-09-15

Applicant: Alnylam Pharmaceuticals, Inc.

Inventor： Aimee M. Deaton ,  David Erbe

IPC: C12N15/113 ,  C12N9/10 ,  A61P13/04

CPC classification number: C12N15/1137 ,  C12Y101/01027 ,  C12Y101/03015 ,  C12N9/1096 ,  C12Y206/01044 ,  A61P13/04 ,  C12N2310/11 ,  C12N2310/315

Abstract: The present invention provides methods for treating subjects suffering from a kidney stone disease carrying a heterozygous AGXT gene variant, methods for identifying such subjects, and compositions comprising nucleic acid inhibitors, e.g., double stranded ribonucleic acid (dsRNA) agents or single stranded antisense polynucleotide agents targeting lactate dehydrogenase A (LDHA) and/or hydroxyacid oxidase (HAO1), for treating such subjects.

公开(公告)号：US20190249152A1

公开(公告)日：2019-08-15

申请号：US16399488

申请日：2019-04-30

Applicant: Codexis Inc.

Inventor： Ish K. Dhawan ,  Gregory Miller ,  Xiyun Zhang

IPC: C12N9/10 ,  C12P41/00 ,  C12P13/00

CPC classification number: C12N9/1096 ,  C12P13/001 ,  C12P41/006 ,  C12Y206/01 ,  C12Y206/01018 ,  Y02P20/52

Abstract: The present disclosure provides engineered transaminase enzymes having improved properties as compared to a naturally occurring wild-type transaminase enzyme. Also provided are polynucleotides encoding the engineered transaminase enzymes, host cells capable of expressing the engineered transaminase enzymes, and methods of using the engineered transaminase enzymes to synthesize a variety of chiral compounds.

公开(公告)号：US20190062555A1

公开(公告)日：2019-02-28

申请号：US16100119

申请日：2018-08-09

Applicant: INVISTA North America S.á r.l.

Inventor： Alex Van Eck CONRADIE ,  Adriana Leonora BOTES

IPC: C08L77/12 ,  C12P13/00 ,  C12N15/52 ,  C12N9/88 ,  C12N9/02 ,  C12N9/10 ,  C12P7/42 ,  C12P7/18 ,  C12N9/04 ,  C07C209/68 ,  C07C227/32 ,  C08G69/26 ,  C07C227/18 ,  C07C221/00 ,  C07C213/02 ,  C08G69/08 ,  C07C229/24 ,  C07C229/12 ,  C07C223/02 ,  C07C215/08 ,  C07C211/09

CPC classification number: C08L77/12 ,  C07B2200/07 ,  C07C209/68 ,  C07C213/02 ,  C07C221/00 ,  C07C227/18 ,  C07C227/32 ,  C08G69/08 ,  C08G69/26 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/1096 ,  C12N9/88 ,  C12N15/52 ,  C12P7/18 ,  C12P7/42 ,  C12P13/001 ,  C12Y101/01061 ,  C12Y102/99006 ,  C12Y206/01018 ,  C12Y401/01015 ,  C12Y401/01071 ,  C07C229/24 ,  C07C229/12 ,  C07C223/02 ,  C07C215/08 ,  C07C211/09

Abstract: This document describes biochemical pathways for producing 4-hydroxybutyrate, 4-aminobutyrate, putrescine or 1,4-butanediol by forming one or two terminal functional groups, comprised of amine or hydroxyl group, in a C5 backbone substrate such as 2-oxoglutarate or L-glutamate.

公开(公告)号：US20180298354A1

公开(公告)日：2018-10-18

申请号：US15735165

申请日：2016-06-10

Applicant: C-LEcta GmbH

Inventor： Andreas Vogel ,  Daniel Schwarze ,  Rico Czaja ,  Sally Bayer ,  Sebastian Bartsch

IPC: C12N9/10 ,  C12P13/00 ,  C12P7/26

CPC classification number: C12P41/006 ,  C12N9/1096 ,  C12P7/26 ,  C12P13/001 ,  C12Y206/01

Abstract: The invention relates to transaminases that are particularly useful for catalyzing the conversion of amine substrates to ketone products and/or vice versa.

公开(公告)号：US10072150B2

公开(公告)日：2018-09-11

申请号：US14976966

申请日：2015-12-21

Applicant: INVISTA North America S.á.r.l.

Inventor： Alex Van Eck Conradie ,  Adriana Leonora Botes

IPC: C08L77/12 ,  C12P7/18 ,  C12P7/42 ,  C12P13/00 ,  C12N15/52 ,  C12N9/88 ,  C12N9/00 ,  C12N9/10 ,  C07C209/68 ,  C07C213/02 ,  C07C221/00 ,  C07C227/18 ,  C07C227/32 ,  C08G69/08 ,  C08G69/26 ,  C12N9/02 ,  C12N9/04

CPC classification number: C08L77/12 ,  C07B2200/07 ,  C07C209/68 ,  C07C213/02 ,  C07C221/00 ,  C07C227/18 ,  C07C227/32 ,  C08G69/08 ,  C08G69/26 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/1096 ,  C12N9/88 ,  C12N15/52 ,  C12P7/18 ,  C12P7/42 ,  C12P13/001 ,  C12Y101/01061 ,  C12Y102/99006 ,  C12Y206/01018 ,  C12Y401/01015 ,  C12Y401/01071 ,  C07C229/24 ,  C07C229/12 ,  C07C223/02 ,  C07C215/08 ,  C07C211/09

Abstract: This document describes biochemical pathways for producing 4-hydroxybutyrate, 4-aminobutyrate, putrescine or 1,4-butanediol by forming one or two terminal functional groups, comprised of amine or hydroxyl group, in a C5 backbone substrate such as 2-oxoglutarate or L-glutamate.

公开(公告)号：US20180195076A1

公开(公告)日：2018-07-12

申请号：US15914308

申请日：2018-03-07

Applicant: Genomatica, Inc.

Inventor： Priti Pharkya

IPC: C12N15/52 ,  C12N15/70 ,  C12N9/88 ,  C12N9/10 ,  C12P13/00

CPC classification number: C12N15/52 ,  C12N9/1096 ,  C12N9/88 ,  C12N15/70 ,  C12P13/001 ,  C12Y206/01085 ,  C12Y401/01024 ,  C12Y401/03038

Abstract: A non-naturally occurring microbial organism having an aniline pathway includes at least one exogenous nucleic acid encoding an aniline pathway enzyme expressed in a sufficient amount to produce aniline. The aniline pathway includes (1) an aminodeoxychorismate synthase, an aminodeoxychorismate lyase, and a 4-aminobenzoate carboxylyase or (2) an anthranilate synthase and an anthranilate decarboxylase. A method for producing aniline, includes culturing these non-naturally occurring microbial organisms under conditions and for a sufficient period of time to produce aniline.