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11.发明申请Expression of Class 2 Mannosidase and Class III Mannosidase in Lower Eukaryotic Cells 有权2种类型的Mannosidase和III类Mannosidase在低等真核细胞中的表达公开(公告)号：US20100062516A1
公开(公告)日：2010-03-11
申请号：US12536911
申请日：2009-08-06
申请人： Stephen Hamilton
发明人： Stephen Hamilton
CPC分类号： C12P21/005 , A01K2217/075 , C12N9/1051 , C12N9/2488 , C12N15/79 , C12Y302/01024 , C12Y302/01114
摘要： A method for producing human-like glycoproteins by expressing a Class 2 α-mannosidase having a substrate specificity for Manα1,3 and Manα1,6 glycosidic linkages in a lower eukaryote is disclosed. Hydrolysis of these linkages on oligosaccharides produces substrates for further N-glycan processing in the secretory pathway.
摘要翻译： 公开了通过在低等真核生物中表达具有Manα1,3和Manα1,6糖苷键的底物特异性的2级α-甘露糖苷酶来生产人样糖蛋白的方法。 在寡糖上的这些键合的水解产生用于在分泌途径中进一步N-聚糖加工的底物。
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12.发明申请公开(公告)号：US20150337273A1
公开(公告)日：2015-11-26
申请号：US14641002
申请日：2015-03-06
申请人： OXYRANE UK LIMITED
CPC分类号： C12N9/1051 , C07K16/32 , C07K2317/14 , C07K2317/41 , C12N9/2402 , C12N9/2488 , C12N9/60 , C12N15/81 , C12P21/005 , C12Y204/01 , C12Y204/01133 , C12Y302/01024 , C12Y302/01084 , C12Y302/01114
摘要： Described herein are methods and genetically engineered fungal cells useful for producing target molecules containing mammalian-like complex N-glycans or containing intermediates in a mammalian glycosyiation pathway.
摘要翻译： 本文描述了可用于产生含有哺乳动物样复合N-聚糖或含有哺乳动物糖基化途径中的中间体的靶分子的方法和遗传工程真菌细胞。
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公开(公告)号：US08299228B2
公开(公告)日：2012-10-30
申请号：US12536812
申请日：2009-08-06
申请人： Stephen Hamilton
发明人： Stephen Hamilton
CPC分类号： C12P21/005 , A01K2217/075 , C12N9/1051 , C12N9/2488 , C12N15/79 , C12Y302/01024 , C12Y302/01114
摘要： A method for producing human-like glycoproteins by expressing a Class 2 α-mannosidase having a substrate specificity for Manα1,3 and Manα1,6 glycosidic linkages in a lower eukaryote is disclosed. Hydrolysis of these linkages on oligosaccharides produces substrates for further N-glycan processing in the secretory pathway.
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公开(公告)号：US20100062523A1
公开(公告)日：2010-03-11
申请号：US12536812
申请日：2009-08-06
申请人： Stephen Hamilton
发明人： Stephen Hamilton
CPC分类号： C12P21/005 , A01K2217/075 , C12N9/1051 , C12N9/2488 , C12N15/79 , C12Y302/01024 , C12Y302/01114
摘要： A method for producing human-like glycoproteins by expressing a Class 2 α-mannosidase having a substrate specificity for Manα1,3 and Manα1,6 glycosidic linkages in a lower eukaryote is disclosed. Hydrolysis of these linkages on oligosaccharides produces substrates for further N-glycan processing in the secretory pathway.
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15.发明申请Expression of class 2 mannosidase and class III mannosidase in lower eukaryotic cells 有权2型甘露糖苷酶和III型甘露糖苷酶在低等真核细胞中的表达公开(公告)号：US20040230042A1
公开(公告)日：2004-11-18
申请号：US10616082
申请日：2003-07-08
发明人： Stephen Hamilton
IPC分类号： C07K014/47 , C07H021/04 , C12N001/18 , C12N015/74
CPC分类号： C12P21/005 , A01K2217/075 , C12N9/1051 , C12N9/2488 , C12N15/79 , C12Y302/01024 , C12Y302/01114
摘要： A method for producing human-like glycoproteins by expressing a Class 2 null-mannosidase having a substrate specificity for Mannull1,3 and Mannull1,6 glycosidic linkages in a lower eukaryote is disclosed. Hydrolysis of these linkages on oligosaccharides produces substrates for further N-glycan processing in the secretory pathway.
摘要翻译： 公开了通过在低等真核生物中表达具有Manalpha1,3和Manalpha1,6糖苷键的底物特异性的2类α-甘露糖苷酶来生产人样糖蛋白的方法。 在寡糖上的这些键合的水解产生用于在分泌途径中进一步N-聚糖加工的底物。
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公开(公告)号：US20040018588A1
公开(公告)日：2004-01-29
申请号：US10185475
申请日：2002-06-26
发明人： Roland Contreras , Nico L.M. Callewaert , Steven C. J. Geysens , Vladimir Kaigorodov , Vervecken Wouter
IPC分类号： C12P021/02 , C12N009/24 , C07H021/04 , C12N001/18 , C12N015/74
CPC分类号： C12Y302/01114 , C12N9/1288 , C12N9/2488 , C12N15/815 , C12Y302/01024
摘要： The present invention provides genetically engineered strains of methylotrophic yeast including Pichia and especially Pichia pastoris capable of producing proteins with reduced or modified glycosylation. Methods of producing glycoproteins with reduced and/or modified glycosylation using such genetically engineered strains of Pichia are also provided. Vectors, which comprise coding sequences for null-1,2-mannosidase I, glucosidase II, GlcNAc-tranferase I and mannosidase II or comprising OCH1 disrupting sequence, for transforming methylotrophic yeasts are contemplated by the present invention. Kit for providing the comtemplated vectors are also included in this invention.
摘要翻译： 本发明提供了遗传工程改造的甲基营养酵母菌株，包括毕赤酵母，特别是巴斯德毕赤酵母能够产生具有降低或修饰的糖基化的蛋白质。 还提供了使用这种遗传工程改造的毕赤酵母菌株生产具有还原和/或修饰的糖基化的糖蛋白的方法。 包含用于转化甲基营养酵母的α-1,2-甘露糖苷酶I，葡糖苷酶II，GlcNAc转移酶I和甘露糖苷酶II或包含OCH1破坏序列的编码序列的载体被本发明考虑。 用于提供comtemplated载体的试剂盒也包括在本发明中。
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17.发明授权Fusion constructs and use of same to produce antibodies with increased Fc receptor binding affinity and effector function 有权融合构建体及其使用以产生具有增加的Fc受体结合亲和力和效应子功能的抗体公开(公告)号：US08859234B2
公开(公告)日：2014-10-14
申请号：US13759925
申请日：2013-02-05
申请人： Roche GlycArt AG
发明人： Pablo Umaña , Peter Bruenker , Claudia Ferrara , Tobias Suter
IPC分类号： C12P21/04 , C12P21/08 , C07K16/28 , C12N9/24 , C07K16/30 , C12N15/62 , C12N9/10 , C07K16/32 , C12P21/00 , A61K38/00 , A61K39/00
CPC分类号： C07K16/2887 , A61K38/00 , A61K2039/505 , C07K16/28 , C07K16/2863 , C07K16/2896 , C07K16/30 , C07K16/32 , C07K2317/24 , C07K2317/41 , C07K2317/732 , C07K2319/05 , C12N9/1051 , C12N9/2488 , C12N15/62 , C12P21/005 , C12Y204/01038 , C12Y204/01144 , C12Y302/01024 , C12Y302/01096 , C12Y302/01114
摘要： The present invention relates to the field of glycosylation engineering of proteins. More particularly, the present invention relates to nucleic acid molecules, including fusion constructs, having catalytic activity and the use of same in glycosylation engineering of host cells to generate polypeptides with improved therapeutic properties, including antibodies with increased Fc receptor binding and increased effector function.
摘要翻译： 本发明涉及蛋白质的糖基化工程领域。 更具体地说，本发明涉及具有催化活性的核酸分子，包括具有催化活性的核糖分子，以及其在宿主细胞的糖基化工程中的用途，以产生具有改善的治疗性质的多肽，包括具有增加的Fc受体结合和增加的效应子功能的抗体。
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18.发明申请公开(公告)号：US20130053550A1
公开(公告)日：2013-02-28
申请号：US13510527
申请日：2010-11-19
CPC分类号： C12N9/1051 , C07K16/32 , C07K2317/14 , C07K2317/41 , C12N9/2402 , C12N9/2488 , C12N9/60 , C12N15/81 , C12P21/005 , C12Y204/01 , C12Y204/01133 , C12Y302/01024 , C12Y302/01084 , C12Y302/01114
摘要： Described herein are methods and genetically engineered fungal cells useful for producing target molecules containing mammalian-like complex N-glycans or containing intermediates in a mammalian glycosylation pathway.
摘要翻译： 本文描述了可用于产生含有哺乳动物样复合N-聚糖的靶分子或在哺乳动物糖基化途径中含有中间体的遗传工程真菌细胞。
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19.发明申请METHOD FOR GENERATING HYPOALLERGENIC GLYCOPROTEINS IN MUTATED OR TRANSGENIC PLANTS OR PLANT CELLS, AND MUTATED OR TRANSGENIC PLANTS AND PLANT CELLS FOR GENERATING HYPOALLERGENIC GLYCOPROTEINS 审中-公开用于在突变或转基因植物或植物细胞和用于产生高致病性糖蛋白的突变或转基因植物和植物细胞中产生低磷酸性谷氨酸的方法公开(公告)号：US20110047657A1
公开(公告)日：2011-02-24
申请号：US12990497
申请日：2009-04-25
CPC分类号： C12P21/005 , C12N9/1051 , C12N9/2402 , C12N15/8257 , C12N15/8258 , C12Y204/01065 , C12Y302/01045 , C12Y302/01114
摘要： Method for providing a hypoallergenic glycoprotein includes growing at least one of a mutated plant, a part of the mutated plant, plant cells produced from the mutated plant, a transgenic plant, a part of the transgenic plant and plant cells produced from the transgenic plant wherein an activity of an enzyme Golgi α-mannosidase II has been eliminated or decreased so as to obtain a grown material. The hypoallergenic glycoprotein is isolated from the grown material.
摘要翻译： 提供低变应原性糖蛋白的方法包括生长突变植物，部分突变植物，从突变植物产生的植物细胞，转基因植物，部分转基因植物和由转基因植物产生的植物细胞中的至少一种，其中 已经消除或减少酶高尔基体α-甘露糖苷酶II的活性，以获得生长的材料。 从生长的材料中分离低变应原性糖蛋白。
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公开(公告)号：US07252933B2
公开(公告)日：2007-08-07
申请号：US10185475
申请日：2002-06-26
申请人： Roland Contreras , Nico L. M. Callewaert , Steven C. J. Geysens , Vladimir Kaigorodov , Vervecken Wouter
发明人： Roland Contreras , Nico L. M. Callewaert , Steven C. J. Geysens , Vladimir Kaigorodov , Vervecken Wouter
CPC分类号： C12Y302/01114 , C12N9/1288 , C12N9/2488 , C12N15/815 , C12Y302/01024
摘要： The present invention provides genetically engineered strains of methylotrophic yeast including Pichia and especially Pichia pastoris capable of producing proteins with reduced or modified glycosylation. Methods of producing glycoproteins with reduced and/or modified glycosylation using such genetically engineered strains of Pichia are also provided. Vectors, which comprise coding sequences for α-1,2-mannosidase I, glucosidase II, GlcNAc-tranferase I and mannosidase II or comprising OCH1 disrupting sequence, for transforming methylotrophic yeasts are contemplated by the present invention. Kit for providing the comtemplated vectors are also included in this invention.
摘要翻译： 本发明提供了遗传工程改造的甲基营养酵母菌株，包括毕赤酵母，特别是巴斯德毕赤酵母能够产生具有降低或修饰的糖基化的蛋白质。 还提供了使用这种遗传工程改造的毕赤酵母菌株生产具有还原和/或修饰的糖基化的糖蛋白的方法。 包含用于转化甲基营养酵母的α-1,2-甘露糖苷酶I，葡糖苷酶II，GlcNAc转移酶I和甘露糖苷酶II或包含OCH1破坏序列的编码序列的载体被本发明考虑。 用于提供comtemplated载体的试剂盒也包括在本发明中。
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