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    Protein glycosylation modification in methylotrophic yeast
    2.
    发明授权
    Protein glycosylation modification in methylotrophic yeast 有权
    甲基营养酵母中的蛋白质糖基化修饰

    公开(公告)号：US07252933B2

    公开(公告)日：2007-08-07

    申请号：US10185475

    申请日：2002-06-26

    申请人： Roland Contreras ,  Nico L. M. Callewaert ,  Steven C. J. Geysens ,  Vladimir Kaigorodov ,  Vervecken Wouter

    发明人： Roland Contreras ,  Nico L. M. Callewaert ,  Steven C. J. Geysens ,  Vladimir Kaigorodov ,  Vervecken Wouter

    IPC分类号： C12Q1/68 ,  C12N15/04 ,  C12N15/63 ,  C07H21/04

    CPC分类号： C12Y302/01114 ,  C12N9/1288 ,  C12N9/2488 ,  C12N15/815 ,  C12Y302/01024

    摘要： The present invention provides genetically engineered strains of methylotrophic yeast including Pichia and especially Pichia pastoris capable of producing proteins with reduced or modified glycosylation. Methods of producing glycoproteins with reduced and/or modified glycosylation using such genetically engineered strains of Pichia are also provided. Vectors, which comprise coding sequences for α-1,2-mannosidase I, glucosidase II, GlcNAc-tranferase I and mannosidase II or comprising OCH1 disrupting sequence, for transforming methylotrophic yeasts are contemplated by the present invention. Kit for providing the comtemplated vectors are also included in this invention.

    摘要翻译： 本发明提供了遗传工程改造的甲基营养酵母菌株，包括毕赤酵母，特别是巴斯德毕赤酵母能够产生具有降低或修饰的糖基化的蛋白质。 还提供了使用这种遗传工程改造的毕赤酵母菌株生产具有还原和/或修饰的糖基化的糖蛋白的方法。 包含用于转化甲基营养酵母的α-1,2-甘露糖苷酶I，葡糖苷酶II，GlcNAc转移酶I和甘露糖苷酶II或包含OCH1破坏序列的编码序列的载体被本发明考虑。 用于提供comtemplated载体的试剂盒也包括在本发明中。

    Protein glycosylation modification in Pichia pastoris
    5.
    发明授权
    Protein glycosylation modification in Pichia pastoris 有权
    巴斯德毕赤酵母中的蛋白质糖基化修饰

    公开(公告)号：US06803225B2

    公开(公告)日：2004-10-12

    申请号：US09896594

    申请日：2001-06-29

    申请人： Roland Contreras ,  Nico L. M. Callewaert ,  Steven C. J. Geysens

    发明人： Roland Contreras ,  Nico L. M. Callewaert ,  Steven C. J. Geysens

    IPC分类号： C12N114

    CPC分类号： C12P21/005 ,  A61K38/00 ,  C12N9/2402 ,  C12N9/2488 ,  C12N15/81 ,  C12N15/815 ,  C12R1/84 ,  C12Y302/0102 ,  C12Y302/01024 ,  Y02P20/52

    摘要： The present invention provides genetically engineered strains of Pichia capable of producing proteins with reduced glycosylation. In particular, the genetically engineered strains of the present invention are capable of expressing either or both of an (&agr;-1,2-mannosidase and glucosidase II. The genetically engineered strains of the present invention can be further modified such that the OCH1 gene is disrupted. Methods of producing glycoproteins with reduced glycosylation using such genetically engineered stains of Pichia are also provided.

    摘要翻译： 本发明提供了能够产生糖基化降低的蛋白质的遗传工程的毕赤酵母菌株。 特别地，本发明的遗传工程改造的菌株能够表达（α-1,2-甘露糖苷酶和葡糖苷酶II）中的一种或两种，本发明的遗传工程改造的菌株可进一步修饰，使得OCH1基因为 还提供了使用这种遗传工程化的毕赤酵母染色体产生糖基化降低的糖蛋白的方法。

    Cells producing glycoproteins having altered glycosylation patterns and method and use thereof
    10.
    发明授权
    Cells producing glycoproteins having altered glycosylation patterns and method and use thereof 有权
    产生具有改变的糖基化模式的糖蛋白的细胞及其方法和用途的细胞

    公开(公告)号：US08815580B2

    公开(公告)日：2014-08-26

    申请号：US12737719

    申请日：2009-08-10

    申请人： Nico L. M. Callewaert ,  Karen De Pourcq ,  Steven Geysens ,  Leander Meuris

    发明人： Nico L. M. Callewaert ,  Karen De Pourcq ,  Steven Geysens ,  Leander Meuris

    IPC分类号： C12N15/79 ,  C12N15/87 ,  C12N15/56 ,  C12N9/24 ,  A61K38/00

    CPC分类号： C12P21/005 ,  C07K2319/02 ,  C12N9/2402 ,  C12Y302/01096 ,  C12Y302/01113

    摘要： The present application relates to the field of glyco-engineering, more specifically to eukaryotic cells wherein both an endoglucosaminidase and a glycoprotein are present. These cells can be used to deglycosylate or partly deglycosylate the (exogenous) glycoprotein, in particular without the need for adding an extra enzyme. Methods are also provided for the application of these cells in protein production. According to one specific aspect, the eukaryotic cells and methods are glyco-engineered yeast cells in which additionally at least one exogenous enzyme needed for complex glycosylation is present, e.g. allowing easier separation of differentially glycosylated glycoproteins.

    摘要翻译： 本申请涉及糖工程领域，更具体地涉及其中存在内切葡糖胺酶和糖蛋白的真核细胞。 这些细胞可用于对（外源性）糖蛋白进行去糖基化或部分脱磺基化，特别是不需要添加额外的酶。 还提供了用于在蛋白质生产中应用这些细胞的方法。 根据一个具体方面，真核细胞和方法是糖工程酵母细胞，其中还存在复合糖基化所需的至少一种外源酶，例如， 允许更容易分离差异糖基化的糖蛋白。