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    EXPRESSION OF CLASS 2 MANNOSIDASE AND CLASS III MANNOSIDASE IN LOWER EUKARYOTIC CELLS
    4.
    发明申请
    EXPRESSION OF CLASS 2 MANNOSIDASE AND CLASS III MANNOSIDASE IN LOWER EUKARYOTIC CELLS 审中-公开
    在较低的原核细胞中表达2类曼卡塞菌素和第3类芒果苷

    公开(公告)号：US20130266981A1

    公开(公告)日：2013-10-10

    申请号：US13624199

    申请日：2012-09-21

    申请人： GlycoFi, Inc.

    发明人： Stephen Hamilton

    IPC分类号： C12P21/00

    CPC分类号： C12P21/005 ,  A01K2217/075 ,  C12N9/1051 ,  C12N9/2488 ,  C12N15/79 ,  C12Y302/01024 ,  C12Y302/01114

    摘要： A method for producing human-like glycoproteins by expressing a Class 2 α-mannosidase having a substrate specificity for Manα1,3 and Manα1,6 glycosidic linkages in a lower eukaryote is disclosed. Hydrolysis of these linkages on oligosaccharides produces substrates for further N-glycan processing in the secretory pathway.

    摘要翻译： 公开了通过在低等真核生物中表达具有Manalpha1,3和Manalpha1,6糖苷键的底物特异性的2类α-甘露糖苷酶来生产人样糖蛋白的方法。 在寡糖上的这些键合的水解产生用于在分泌途径中进一步N-聚糖加工的底物。

    Protein glycosylation modification in methylotrophic yeast
    6.
    发明申请
    Protein glycosylation modification in methylotrophic yeast 有权
    甲基营养酵母中的蛋白质糖基化修饰

    公开(公告)号：US20080009037A1

    公开(公告)日：2008-01-10

    申请号：US11827998

    申请日：2007-07-13

    申请人： Roland Contreras ,  Nico Callewaert ,  Steven Geysens ,  Vladimir Kaigorodov ,  Vervecken Wouter

    发明人： Roland Contreras ,  Nico Callewaert ,  Steven Geysens ,  Vladimir Kaigorodov ,  Vervecken Wouter

    IPC分类号： C12P21/02 ,  C12N1/19

    CPC分类号： C12Y302/01114 ,  C12N9/1288 ,  C12N9/2488 ,  C12N15/815 ,  C12Y302/01024

    摘要： The present invention provides genetically engineered strains of methylotrophic yeast including Pichia and especially Pichia pastoris capable of producing proteins with reduced or modified glycosylation. Methods of producing glycoproteins with reduced and/or modified glycosylation using such genetically engineered strains of Pichia are also provided. Vectors, which comprise coding sequences for α-1,2-mannosidase I, glucosidase II, GlcNAc-tranferase I and mannosidase II or comprising OCH1 disrupting sequence, for transforming methylotrophic yeasts are contemplated by the present invention. Kit for providing the comtemplated vectors are also included in this invention.

    摘要翻译： 本发明提供了遗传工程改造的甲基营养酵母菌株，包括毕赤酵母，特别是巴斯德毕赤酵母能够产生具有降低或修饰的糖基化的蛋白质。 还提供了使用这种遗传工程改造的毕赤酵母菌株生产具有还原和/或修饰的糖基化的糖蛋白的方法。 包含用于转化甲基营养酵母的α-1,2-甘露糖苷酶I，葡糖苷酶II，GlcNAc转移酶I和甘露糖苷酶II或包含OCH1破坏序列的编码序列的载体被本发明考虑。 用于提供comtemplated载体的试剂盒也包括在本发明中。