摘要:
A method for enhancing contrast in fluorescence imaging is provided. The method comprises providing a patterned illumination source for illuminating one or more regions corresponding to a scan step, scanning at least a portion of a surface of a subject using a plurality of scan steps, acquiring image frames corresponding to two or more scan steps, deducting a background fluorescence from the image frames corresponding to the two or more scan steps to form one or more processed image frames, and reconstructing an image using one or more of the processed image frames.
摘要:
The invention concerns methods and apparatuses for quantitatively determining the concentration of fluorophores of a substance in a sample. A constant portion of the reference light of a reference light wave length (λr) emitted by a reference light source is coupled in by an optical element in the direction of a receiving element. A first value corresponding to the portion of the reference light coupled in which is incident on the receiving element is detected. The sample is irradiated with the excitation light of an excitation wave length (λex) emitted by an excitation light source. A second value corresponding to the portion of the fluorescent light of an emission wave length (λem) emitted by the sample which is incident on the receiving element. The ratio of the second value to the first value is determined. The number of fluorophores in the substance is determined based on the ratio.
摘要:
Methods and apparatus, including computer program products, implementing and using techniques for collecting optical data pertaining to one or more characteristics of a sample. The apparatus has a light source, one or more illumination optical elements, a scanner, one or more collection optical elements, and a device forming an aperture that limits detection of light from the sample. The illumination optical elements direct a light beam from the light source onto the sample. The scanner scans the light beam across the sample. The collection optical elements collect light from the sample and transmit the collected light to a detector. None of the collection optical elements are included among the illumination optical elements. The device forming an aperture limits detection of light from the sample to light associated with a limited vertical depth within the sample, and is one of the collection optical elements.
摘要:
A surface light source device includes a light source body, a partition member, an isolating member and a voltage applying part. The light source body has an internal space into which discharge gas is injected. The partition wall divides the internal space into discharge spaces. The partition wall has a connection hole that connects the discharge spaces with each other. The isolating member is disposed such that the isolating member corresponds to the connection hole. The isolating member seals the connection hole to isolate the discharge spaces from each other. The voltage applying part induces discharge of the discharge gas in the discharge spaces. Thus, current drift is prevented.
摘要:
A light emitting device in which annular self luminous light sources are supported intermediate the ends of a resilient rod. In an illustrated embodiment a plurality of the rods are located concentrically within a transparent tube between end caps which close the ends of the tube.
摘要:
In a preferred embodiment, a radioluminescent lamp having a glass face plate with a plurality of parallel planar light guides, each preferably having a transparent glass, sapphire or quartz base member, disposed perpendicularly with respect to the glass face plate and coated on both sides with a thin film of radioluminescent phosphor material. The plates are mounted in a sealed envelope filled with tritium gas, the radioactive decay of the tritium causing the phosphor to luminesce. The phosphor material and all but one of the edges of each light guide are overcoated with a reflective material, such as aluminum, to guide the generated light to a single edge of the light guide, which edge is adjacent the glass face plate. The phosphor is preferably a calcium sulfide-based material forming a continuous, binder-free layer on the transparent base member. The resulting structure has a high phosphor-surface-area to tritium-gas-volume ratio and directional light guiding, yielding a substantially higher output light density than conventional radioluminescent lamps.
摘要:
This invention relates to a means for more efficiently and more safely providing self-luminous lighting devices for use in signs, markers, indicators and the like. Said invention provides self luminosity by means of a plurality of glass microspheres containing both a light emitting phosphor and a radioactive gas. The "soft" emission of electrons from the beta emitting gas can not penetrate the glass wall of the microspheres, thereby constituting no radiation hazard. The containment of the self-luminous system within an envelope which does not pass any radiation prevents the deterioration of nearby organic materials. A further advantage of said invention is that the plurality of individual containment envelopes minimize the escape of radioactive gas in the event of any physical damage to an assembly of such envelopes. A still further advantage of my invention is that the radioactive gas completely surrounds the phosphor particles, thus causing light emission from 100 percent of the surface of said particles.
摘要:
A radiation excited self-luminous light source in which luminescence is provided by the impingement of beta emissions upon a phosphor within the source. A gastight chamber within the source is bounded by a concave phosphor surface and by radiationresistant window. The concave surface is of a predetermined shape designed to maximize the efficiency of the source. In addition, the gastight chamber is designed to withstand pressures of up to 15 atmospheres, thereby enabling gaseous radioisotopes of greater activity to be utilized as the source of beta emissions. In one embodiment, greater efficiency is achieved by utilizing a window having a convex surface which corresponds to the concave phosphor surface and which is positioned a predetermined distance from the phosphor surface equal to the average range of beta emissions within the chamber.
摘要:
A high intensity discharge lamp includes an electrically insulating arc tube. A sealed shroud encloses the arc tube. An electrically conductive frame member is disposed inside the shroud and is electrically connected to an electrical conductor that extends in the arc tube. Electrically conductive foil is fastened to the frame member and forms a closed loop that encircles a leg of the arc tube by an angle in a range of at least 270 degrees to 360 degrees. The foil can be connected to the frame member and to itself. A distance from an outer surface of a flange of the arc tube leg to a proximal edge of the foil can range from 1.5 to 8 mm. A width of the foil can range from 1 mm to 4 mm.