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公开(公告)号:US07105303B2
公开(公告)日:2006-09-12
申请号:US09929782
申请日:2001-08-13
申请人: Robert O. Ralston , Frank Marcus , Kent B. Thudium , Barbara A. Gervase , John A. Hall , Kim M. Berger , Qui-Lim Choo , Michael Houghton , George Kuo
发明人: Robert O. Ralston , Frank Marcus , Kent B. Thudium , Barbara A. Gervase , John A. Hall , Kim M. Berger , Qui-Lim Choo , Michael Houghton , George Kuo
IPC分类号: G01N33/53
CPC分类号: C07K14/005 , A61K39/00 , C12N2770/24222 , Y10S530/82 , Y10S530/826 , Y10S977/802 , Y10S977/803 , Y10S977/804 , Y10S977/915 , Y10S977/918
摘要: Two Hepatitis C Virus envelope proteins (E1 and E2) are expressed without sialylation. Recombinant expression of these proteins in lower eukaryotes, or in mammalian cells in which terminal glycosylation is blocked, results in recombinant proteins which are more similar to native HCV glycoproteins. When isolated by GNA lectin affinity, the E1 and E2 proteins aggregate into virus-like particles.
摘要翻译: 两种丙型肝炎病毒包膜蛋白(E1和E2)不表达唾液酸化。 这些蛋白质在低等真核生物或其末端糖基化被阻断的哺乳动物细胞中的重组表达导致与天然HCV糖蛋白更相似的重组蛋白质。 当通过GNA凝集素亲和力分离时,E1和E2蛋白聚集成病毒样颗粒。
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公开(公告)号:US06465231B2
公开(公告)日:2002-10-15
申请号:US09916109
申请日:2001-07-25
申请人: Stephen D. Harrison , John A. Hall , Maria Calderon-Cacia , Ziyang Zhong , Eric Y. Fang , Doris G. Coit , Steve H. Nguyen , Angelica Medina-Selby
发明人: Stephen D. Harrison , John A. Hall , Maria Calderon-Cacia , Ziyang Zhong , Eric Y. Fang , Doris G. Coit , Steve H. Nguyen , Angelica Medina-Selby
IPC分类号: C12N912
CPC分类号: C12N9/1205 , C12Q1/485 , C12Y207/01037 , G01N2500/00
摘要: The invention provides truncated GSK3 polypeptides capable of crystallization, including GSK3&agr; and GSK3&bgr; polypeptides, and use of these polypeptides to identify and optimize GSK3 inhibitors. Also provided are GSK3 polypeptides having at least one substituted amino acid that differs from wild-type GSK3, wherein the substituted amino acid is incapable of being phosphorylated. The invention finds use in providing methods of identifying and optimizing compounds useful for treating diseases mediated by GSK3 activity, including Alzheimer's disease, type 2 diabetes, and inflammation.
摘要翻译: 本发明提供能够结晶的截短的GSK3多肽,包括GSK3α和GSK3β多肽,以及使用这些多肽来鉴定和优化GSK3抑制剂。 还提供了具有与野生型GSK3不同的至少一个取代的氨基酸的GSK3多肽,其中取代的氨基酸不能被磷酸化。 本发明用于提供鉴定和优化用于治疗由GSK3活性介导的疾病(包括阿尔茨海默病,2型糖尿病和炎症)的化合物的方法。
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公开(公告)号:US5942234A
公开(公告)日:1999-08-24
申请号:US443260
申请日:1995-05-17
申请人: Robert O. Ralston , Frank Marcus , Kent B. Thudium , Barbara A. Gervase , John A. Hall , Kim M. Berger , Qui-Lim Choo , Michael Houghton , George Kuo
发明人: Robert O. Ralston , Frank Marcus , Kent B. Thudium , Barbara A. Gervase , John A. Hall , Kim M. Berger , Qui-Lim Choo , Michael Houghton , George Kuo
IPC分类号: G01N33/576 , A61K35/16 , A61K38/00 , A61K38/10 , A61K39/00 , A61K39/29 , A61P1/16 , A61P31/12 , C07K14/18 , C12N1/19 , C12N5/10 , C12N15/09 , C12N15/51 , C12P21/02 , C12P21/04 , C07K1/00 , C12Q1/70
CPC分类号: C07K14/005 , A61K39/00 , C12N2770/24222 , Y10S530/82 , Y10S530/826 , Y10S977/802 , Y10S977/803 , Y10S977/804 , Y10S977/915 , Y10S977/918
摘要: Two Hepatitis C Virus envelope proteins (E1 and E2) are expressed without sialylation. Recombinant expression of these proteins in lower eukaryotes, or in mammalian cells in which terminal glycosylation is blocked, results in recombinant proteins which are more similar to native HCV glycoproteins. When isolated by GNA lectin affinity, the E1 and E2 proteins aggregate into virus-like particles.
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