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    Retroviral vectors pseudotyped with SRV-3 envelope glycoprotein sequences
    4.
    发明授权
    Retroviral vectors pseudotyped with SRV-3 envelope glycoprotein sequences 失效
    用SRV-3包膜糖蛋白序列假型分型的逆转录病毒载体

    公开(公告)号：US5932467A

    公开(公告)日：1999-08-03

    申请号：US726346

    申请日：1996-10-03

    申请人： Mohammad Ayub Khan ,  Robert O. Ralston ,  John E. Murphy

    发明人： Mohammad Ayub Khan ,  Robert O. Ralston ,  John E. Murphy

    IPC分类号： C12N15/09 ,  C07K14/15 ,  C12N5/10 ,  C12N7/00 ,  C12N15/867 ,  A01N63/00 ,  C12N15/00 ,  C12P21/04

    CPC分类号： C12N15/86 ,  C07K14/005 ,  C12N2740/13022 ,  C12N2740/13043 ,  C12N2800/108

    摘要： Cells producing recombinant retroviral particles are provided. The cells contain a first vector having a coding region encoding retroviral LTRs and a packaging signal under the control of an expression control system, a tRNA binding site located upstream from the packaging signal and origin of second strand DNA synthesis located downstream from the packaging signal. The cells also contain a second vector having a coding region encoding retroviral capsid proteins gag and pol under the control of an expression control system and a third vector having a coding region encoding a simian type D retrovirus envelope glycoprotein under the control of an expression control system.

    摘要翻译： 提供产生重组逆转录病毒颗粒的细胞。 细胞含有具有编码逆转录病毒LTR的编码区和在表达控制系统控制下的包装信号的第一载体，位于包装信号上游的tRNA结合位点和位于包装信号下游的第二链DNA合成起源。 细胞还含有第二载体，其在表达控制系统的控制下具有编码逆转录病毒衣壳蛋白gag和pol的编码区，第三载体具有在表达控制系统的控制下编码猿类D型逆转录病毒包膜糖蛋白的编码区 。

    HIV GENE CLONING STRATEGY
    6.
    发明申请
    HIV GENE CLONING STRATEGY 审中-公开
    HIV基因克隆策略

    公开(公告)号：US20080220528A1

    公开(公告)日：2008-09-11

    申请号：US11963007

    申请日：2007-12-21

    申请人： John A. Howe ,  Robert O. Ralston

    发明人： John A. Howe ,  Robert O. Ralston

    IPC分类号： C12N15/74 ,  C12N1/20 ,  C12N15/11

    CPC分类号： C12N15/70

    摘要： The invention relates to methods for cloning Human Immunodeficiency Virus (HIV) genes, in particular HIV envelope genes. The invention also relates to cloning strategies for mapping resistance determinants for HIV genes, in particular HIV envelope genes.

    摘要翻译： 本发明涉及用于克隆人类免疫缺陷病毒（HIV）基因，特别是HIV包膜基因的方法。 本发明还涉及用于测定HIV基因，特别是HIV包膜基因的抗性决定簇的克隆策略。

    Methods and polynucleotide constructs for treating host cells for infection or hyperproliferative disorders
    8.
    发明授权
    Methods and polynucleotide constructs for treating host cells for infection or hyperproliferative disorders 失效
    用于治疗宿主细胞感染或过度增殖性疾病的方法和多核苷酸构建体

    公开(公告)号：US5861290A

    公开(公告)日：1999-01-19

    申请号：US965039

    申请日：1992-10-22

    申请人： Mark A. Goldsmith ,  Robert O. Ralston

    发明人： Mark A. Goldsmith ,  Robert O. Ralston

    IPC分类号： C12N15/09 ,  A61K31/70 ,  A61K35/76 ,  A61K38/55 ,  A61K39/395 ,  A61K48/00 ,  A61P31/12 ,  A61P35/00 ,  C07K14/15 ,  C12N5/10 ,  C12N15/85 ,  C12N15/11 ,  C12N15/63 ,  C12N15/86

    CPC分类号： A61K31/70 ,  C12N15/85 ,  C12N2830/42 ,  C12N2840/20

    摘要： Host cells may be treated for an infection or a hyperproliferative disorder which is characterized by the presence, in the affected cells, of a trans-acting factor capable of regulating gene expression by inserting into the cells a polynucleotide construct having a cis-acting regulatory sequence which is regulated by the trans-acting factor and an effector gene which renders said cell susceptible to protection or destruction. For example, the cis-acting region may be homologous to the HIV tar region, and the effector gene may encode ricin A or HSV-1 thymidine kinase. Upon infection with HIV, the HIV tat protein activates the tar region, and induces transcription and expression of ricin A, resulting in cell death, or of HSV-1 tk, resulting in cell death upon treatment with dideoxynucleoside agents such as acyclovir and gancyclovir.

    摘要翻译： 宿主细胞可以被治疗感染或过度增殖性疾病，其特征在于在受影响的细胞中存在能够通过将具有顺式作用调节序列的多核苷酸构建体插入到细胞中来调节基因表达的反式作用因子 其由反式作用因子和使所述细胞易受保护或破坏的效应基因调节。 例如，顺式作用区可以与HIV焦油区同源，并且效应基因可以编码蓖麻毒蛋白A或HSV-1胸苷激酶。 艾滋病病毒感染后，HIV tat蛋白激活焦油区，诱导蓖麻毒蛋白A的转录和表达，导致细胞死亡或HSV-1 tk，导致二脱氧核苷类药物如阿昔洛韦和加替昔洛韦治疗时细胞死亡。