公开(公告)号：US4619530A

公开(公告)日：1986-10-28

申请号：US815294

申请日：1985-12-24

Applicant: Peter M. Meserol ,  Jesse L. Acker

Inventor： Peter M. Meserol ,  Jesse L. Acker

IPC: G01N21/03 ,  G01N21/25 ,  G01N21/00

CPC classification number: G01N21/0303 ,  G01N21/255

Abstract: The combination of a cuvette for receiving a medium undergoing change in optical characteristics which change modifies the energy level of a ray of energy passing through the medium and wherein the cuvette is provided with integrally formed first and second ray modifying optical means, such as collimating and collecting lens, wherein the first ray modifying optical means receives and modifies the ray in a first manner, such as by collimation, and transmits the ray into the medium and wherein the second ray modifying optical means receives and modifies the ray in a second manner, such as by collection, upon the ray passing through the medium and transmits the ray from the cuvette; and an electrical circuit including photoemissive and photosensitive means, such as a photoemitter and photodetector, wherein the photoemissive means is in intimate optical contact with the first ray modifying optical element of the cuvette and wherein the photosensitive means is in intimate optical contact with the second ray modifying means.

Abstract translation: 用于接收经历光学特性变化的介质的比色杯的组合改变了通过介质的能量射线的能量水平，并且其中比色杯设置有整体形成的第一和第二光线修改光学装置，例如准直和 收集透镜，其中第一光线修改光学装置例如通过准直以第一方式接收和修改光线，并将光线传输到介质中，并且其中第二光线修改光学装置以第二方式接收和修改光线， 例如通过收集，在射线通过介质并透射来自比色杯的光线; 以及包括光发射和感光装置的电路，例如光发射器和光电检测器，其中光发射装置与比色杯的第一光线修改光学元件紧密地光学接触，并且其中光敏装置与第二光线紧密地光学接触 修改手段。

公开(公告)号：US4197088A

公开(公告)日：1980-04-08

申请号：US835996

申请日：1977-09-23

Applicant: Peter M. Meserol ,  Jesse L. Acker

Inventor： Peter M. Meserol ,  Jesse L. Acker

IPC: G01N21/03 ,  G01N21/25 ,  G01N21/59 ,  G01N21/75 ,  G01N21/82 ,  G01N33/48 ,  G01N33/53 ,  G01N33/543 ,  G01N35/00 ,  G01N33/16 ,  G01N21/24

CPC classification number: G01N33/5304 ,  G01N21/03 ,  G01N21/253 ,  G01N21/5911 ,  G01N21/75 ,  G01N33/53 ,  G01N33/5302 ,  G01N33/54366 ,  G01N35/00029 ,  G01N2035/00099 ,  G01N21/82 ,  G01N33/48 ,  Y10S436/805 ,  Y10S436/807 ,  Y10S436/814 ,  Y10S436/818

Abstract: A method for qualitative and quantitative determination of agglutination in immunoreactive systems, and for determining the concentration of the immunoreactive components of such system, which method comprises introducing a fluid biological test specimen and a reagent therefor (e.g., latex spheres) into a reaction zone in an image cell and evenly mixing and incubating the mixture thereby forming agglutinated particles. The reaction zone is then transilluminated and the light beams transmitted therethrough are imaged on an image sensor. Dark areas are formed on the surface of the image sensor which are measured, preferably electronically. This procedure is repeated for a reference specimen and the total dark imaged area is compared with the total dark area obtained for the unknown specimen for qualitative determination.In order to obtain the concentration of the immunoreactive component in the unknown specimen, the foregoing procedure is repeated for at least two specimens with known antibody concentrations, one of which may be a negative control. The concentration of the immunoreactive component of the unknown sample is then determined from the total dark imaged area thereof and the relationship between the total dark imaged areas of the known specimen and their respective concentrations.

Abstract translation: 一种用于定性和定量测定免疫反应性系统中的凝集以及用于确定该系统的免疫反应性组分的浓度的方法，该方法包括将流体生物测试样品及其试剂（例如胶乳球）引入反应区 并将混合物均匀混合并孵育，从而形成凝集的颗粒。 然后将反应区域透过，并将透射的光束成像在图像传感器上。 在图像传感器的表面上，优选以电子方式测量暗区。 对于参考样品重复该过程，并将总暗区成像与未知样品获得的总暗区进行定性测定。 为了获得未知标本中免疫反应成分的浓度，对已知抗体浓度的至少两个样品重复上述步骤，其中一个可能是阴性对照。 然后从其全部暗成像区域确定未知样品的免疫反应性组分的浓度，以及已知样品的总暗成像区域与其相应浓度之间的关系。

公开(公告)号：US4013368A

公开(公告)日：1977-03-22

申请号：US500166

申请日：1974-08-23

Applicant: Jesse L. Acker ,  Peter M. Meserol

Inventor： Jesse L. Acker ,  Peter M. Meserol

IPC: C12M1/34 ,  G01N21/25 ,  G01N21/27 ,  G01N35/00 ,  H03K5/08 ,  H04L25/06 ,  G01N1/10

CPC classification number: G01N35/00594 ,  C12M41/36 ,  G01N21/253 ,  G01N21/274 ,  H03K5/084 ,  H04L25/062 ,  G01N35/00693

Abstract: The apparatus comprises a plurality of stationary cuvettes, a chamber for the fluid to be evaluated, means for placing the chamber in fluid communication with each of the cuvettes and for permitting the flow of fluid from the chamber into each of the cuvettes, a plurality of optical transmitting means in registration with each of the cuvettes for transmitting a beam of radiant energy through each of the cuvettes, and detector means for intercepting each of the beams of radiant energy and for measuring any optical changes in the radiant energy passing through the fluid in each of the cuvettes.The chamber for the fluid, the plurality of cuvettes and the fluid communication means are all housed within, preferably, a disposable, self-contained cartridge of unitary construction. The cartridge is mountable within a carriage that houses the transmitting and detector means of the apparatus. The chamber which forms the upper portion of the cartridge can be prefilled with bacterial suspension. Periodically, during the growth of the subject bacteria the suspensions are agitated by suitable means. Samples of antibiotics of varying potency are placed in the cuvettes. The cartridge is mounted in the carriage. On a signal from the detector means a portion of the suspension passes into each of the cuvettes to begin the antibiotic titer which is evaluated by means of the transmitting and detector means and associated electronic circuitry.

Abstract translation: 该装置包括多个静止反应杯，用于待评估流体的室，用于将该室与每个反应杯流体连通并用于允许流体从腔室流入每个比色皿的装置，多个 与每个比色杯对准的光学传输装置，用于通过每个比色皿透射辐射能束;以及检测器装置，用于拦截每个辐射能量束，并用于测量通过流体的辐射能的任何光学变化 每个比色杯。

公开(公告)号：US3983006A

公开(公告)日：1976-09-28

申请号：US500125

申请日：1974-08-23

Applicant: Jesse L. Acker ,  Peter M. Meserol

Inventor： Jesse L. Acker ,  Peter M. Meserol

IPC: C12M1/34 ,  G01N21/25 ,  G01N21/27 ,  G01N35/00 ,  H03K5/08 ,  H04L25/06 ,  C12K1/04

CPC classification number: G01N35/00594 ,  C12M41/36 ,  G01N21/253 ,  G01N21/274 ,  H03K5/084 ,  H04L25/062 ,  G01N35/00693

Abstract: A method of determining minimum inhibitory concentration (MIC) of an antibiotic by continuously measuring the change in optical properties in response to the bacterial growth rate of a bacterial suspension in the absence and the presence of the antibiotic. The method is carried out in an automatic biological fluid analyzer.

Abstract translation: 一种通过在不存在和存在抗生素的情况下连续测量细菌悬浮液的细菌生长速率来连续测量光学性质的变化来确定抗生素的最小抑制浓度（MIC）的方法。 该方法在自动生物流体分析仪中进行。

公开(公告)号：US3938049A

公开(公告)日：1976-02-10

申请号：US500124

申请日：1974-08-23

Applicant: Jesse L. Acker ,  Peter M. Meserol

Inventor： Jesse L. Acker ,  Peter M. Meserol

IPC: C12M1/34 ,  G01N21/25 ,  G01N21/27 ,  G01N35/00 ,  H03K5/08 ,  H04L25/06 ,  H03B1/00 ,  H03K5/00 ,  H04B1/10

CPC classification number: G01N35/00594 ,  C12M41/36 ,  G01N21/253 ,  G01N21/274 ,  H03K5/084 ,  H04L25/062 ,  G01N35/00693

Abstract: Baseline correction for a pulse train is achieved by arranging the source generating the pulse train in series circuit with a switch and by rendering the switch selectively conductive during time periods coextensive with the separation interval between successive pulses in the pulse train. The switch output is stored and is subtractively combined with the pulse train. Switching control is effected by a signal generator which also time controls generation of the pulse train.

Abstract translation: 脉冲序列的基线校正是通过将源产生脉冲序列与串联的开关进行排列而实现的，并且通过在与脉冲序列中连续脉冲之间的间隔间隔共同延长的时间段期间使开关选择性地导通。 开关输出被存储并与脉冲串相减。 开关控制由时间控制脉冲串的产生的信号发生器来实现。