公开(公告)号：US10316069B2

公开(公告)日：2019-06-11

申请号：US15739057

申请日：2016-06-23

Applicant: Spiber Technologies AB

Inventor： My Hedhammar

IPC: A61K38/00 ,  C07K14/435 ,  C08H1/00 ,  C12N5/00 ,  C12N15/62 ,  C07K16/28

Abstract: A recombinant fusion protein is comprising a spider silk fragment and a cyclic RGD cell-binding motif with selectivity for integrins, such as for α5β1 integrins. The fusion protein is useful as a cell scaffold material and for the cultivation of cells displaying integrins on their cell surface.

公开(公告)号：US20170283474A1

公开(公告)日：2017-10-05

申请号：US15490328

申请日：2017-04-18

Applicant: SPIBER TECHNOLOGIES AB

Inventor： Jan JOHANSSON ,  Anna RISING ,  My HEDHAMMAR ,  Kerstin NORDLING

IPC: C07K14/435 ,  C12N15/62

CPC classification number: C07K14/43518 ,  C12N15/62

Abstract: A method of producing a desired non-spidroin protein or polypeptide is comprising the steps of expressing in a suitable host a fusion protein, obtaining a mixture containing the fusion protein, and optionally isolating the fusion protein. The fusion protein is comprising at least one solubility-enhancing moiety which is derived from the N-terminal (NT) fragment of a spider silk protein. It is further comprising at least one moiety which is a desired non-spidroin protein or polypeptide. Each solubility-enhancing moiety is linked directly or indirectly to the desired protein or polypeptide moiety.

公开(公告)号：US20150087046A1

公开(公告)日：2015-03-26

申请号：US14398370

申请日：2013-05-02

Applicant: SPIBER TECHNOLOGIES AB

Inventor： My Hedhammar

IPC: C07K14/435 ,  C07K14/315 ,  C12N5/00 ,  C07K16/28 ,  C12N9/96 ,  C07K16/12 ,  C07K14/36

CPC classification number: C07K14/43518 ,  C07K1/22 ,  C07K14/315 ,  C07K14/36 ,  C07K16/1271 ,  C07K16/28 ,  C07K2319/02 ,  C07K2319/30 ,  C07K2319/735 ,  C12N5/0081 ,  C12N9/96 ,  C12N15/62 ,  Y10T428/2982 ,  Y10T442/10

Abstract: A recombinant fusion protein comprising the moieties Band CT is provided. B is a non-spidroin moiety which provides the capacity of selective interaction with an organic target. CT is a moiety of from 70 to 120 amino acid residues and is derived from the C-terminal fragment of a spider silk protein. The fusion protein is not comprising any moiety derived from the repetitive fragment of a spider silk protein.

Abstract translation: 提供了包含部分带CT的重组融合蛋白。 B是提供与有机靶的选择性相互作用能力的非宿主部分。 CT是70至120个氨基酸残基的部分，衍生自蜘蛛丝蛋白的C末端片段。 融合蛋白不包含衍生自蜘蛛丝蛋白重复片段的任何部分。

公开(公告)号：US20240033402A1

公开(公告)日：2024-02-01

申请号：US18023614

申请日：2020-09-01

Applicant: Spiber Technologies AB

Inventor： Mona Widhe ,  My Hedhammar ,  Gemma Mestres ,  Anna Diez-Escudero

IPC: A61L27/56 ,  A61L27/22

CPC classification number: A61L27/56 ,  A61L27/227 ,  A61L2300/252 ,  A61L2300/102 ,  A61L2430/02

Abstract: A method for producing macroporous scaffolds which are useful in bone generation is provided. A liquid phase which is an aqueous solution of a non-denatured silk protein is subjected to mechanical foaming. A mineral phase comprising α-tricalcium phosphate (α-TCP) with hydroxyapatite (HA) is blended into the foamed liquid phase during continued mechanical foaming, thereby forming a macroporous paste. The macroporous paste is stabilized into a desired shape. The stabilized, shaped porous paste is mineralized into a macroporous calcium-deficient hydroxyapatite (CDHA)-based scaffold.

公开(公告)号：US20200207819A1

公开(公告)日：2020-07-02

申请号：US16816486

申请日：2020-03-12

Applicant: SPIBER TECHNOLOGIES AB

Inventor： Jan JOHANSSON ,  Anna RISING ,  Nina KRONQVIST ,  Kerstin NORDLING

IPC: C07K14/435 ,  C07K1/36

Abstract: A protein comprising a moiety of 100-160 amino acid residues having at least 70% identity with the N-terminal (NT) fragment of a spider silk protein, wherein the amino acid residue corresponding to position 40 in NT is selected from the group consisting of Lys, Arg and His; and wherein the amino acid residue corresponding to position 65 in NT is selected from the group consisting of Asp and Glu, is useful as a moiety in a fusion protein for enhancing the solubility of another moiety in the fusion protein, which is a desired protein or polypeptide.

公开(公告)号：US10662230B2

公开(公告)日：2020-05-26

申请号：US16403780

申请日：2019-05-06

Applicant: Spiber Technologies AB

Inventor： My Hedhammar

IPC: A61K38/00 ,  C07K14/435 ,  C08H1/00 ,  C12N5/00 ,  C12N15/62 ,  C07K16/28

Abstract: A recombinant fusion protein is comprising a spider silk fragment and a cyclic RGD cell-binding motif with selectivity for integrins, such as for α5β1 integrins. The fusion protein is useful as a cell scaffold material and for the cultivation of cells displaying integrins on their cell surface.

公开(公告)号：US20190144819A1

公开(公告)日：2019-05-16

申请号：US16077179

申请日：2017-02-10

Applicant: SPIBER TECHNOLOGIES AB

Inventor： My HEDHAMMAR ,  Mona WIDHE ,  Ulrika JOHANSSON

IPC: C12N5/00 ,  C07K14/435

Abstract: A cell scaffold material is manufactured by providing an aqueous solution of a silk protein capable of assembling into a water-insoluble macrostructure. The silk protein is mixed with eukaryotic cells, and the silk protein is assembled into a water-insoluble macrostructure in the presence of the cells, thereby forming a scaffold material for cultivating the cells. The cells can be grown integrated with the scaffold material under conditions suitable for cell culture.

公开(公告)号：US20180273590A1

公开(公告)日：2018-09-27

申请号：US15544945

申请日：2016-11-11

Applicant: SPIBER TECHNOLOGIES AB

Inventor： Jan JOHANSSON ,  Anna RISING ,  Nina KRONQVIST ,  Kerstin NORDLING

IPC: C07K14/435 ,  C07K1/36

CPC classification number: C07K14/43518 ,  C07K1/36 ,  C07K2319/00 ,  C07K2319/35

Abstract: A protein comprising a moiety of 100-160 amino acid residues having at least 70% identity with the N-terminal (NT) fragment of a spider silk protein, wherein the amino acid residue corresponding to position 40 in NT is selected from the group consisting of Lys, Arg and His; and wherein the amino acid residue corresponding to position 65 in NT is selected from the group consisting of Asp and Glu, is useful as a moiety in a fusion protein for enhancing the solubility of another moiety in the fusion protein, which is a desired protein or polypeptide.

公开(公告)号：US09856308B2

公开(公告)日：2018-01-02

申请号：US14398361

申请日：2013-05-02

Applicant: SPIBER TECHNOLOGIES AB

Inventor： My Hedhammar ,  Jan Johansson ,  Anna Rising ,  Per Åke Nygren

IPC: C07K14/78 ,  C07K14/43 ,  C07K16/00 ,  C07K16/44 ,  C07K14/435

CPC classification number: C07K14/78 ,  C07K14/43518 ,  C07K16/00 ,  C07K16/44 ,  C07K2317/35 ,  C07K2317/56 ,  C07K2317/569 ,  C07K2317/622 ,  C07K2319/00 ,  C07K2319/30 ,  C07K2319/70

Abstract: A recombinant fusion protein comprising the moieties Band CT, and optionally REP, wherein B is comprising at least one immunoglobulin fragment, which provides the capacity of selective interaction with an organic target; CT is a moiety of from 70 to 120 amino acid residues and is derived from the C-terminal fragment of a spider silk protein; and REP is a moiety of from 70 to 300 amino acid residues and is derived from the repetitive fragment of a spider silk protein.

公开(公告)号：US09708376B2

公开(公告)日：2017-07-18

申请号：US14398370

申请日：2013-05-02

Applicant: SPIBER TECHNOLOGIES AB

Inventor： My Hedhammar

IPC: C07K14/435 ,  C07K19/00 ,  C07K1/22 ,  C12N15/62 ,  C07K14/315 ,  C07K14/36 ,  C07K16/12 ,  C07K16/28 ,  C12N5/00 ,  C12N9/96

CPC classification number: C07K14/43518 ,  C07K1/22 ,  C07K14/315 ,  C07K14/36 ,  C07K16/1271 ,  C07K16/28 ,  C07K2319/02 ,  C07K2319/30 ,  C07K2319/735 ,  C12N5/0081 ,  C12N9/96 ,  C12N15/62 ,  Y10T428/2982 ,  Y10T442/10

Abstract: A recombinant fusion protein comprising the moieties B and CT is provided. B is a non-spidroin moiety which provides the capacity of selective interaction with an organic target. CT is a moiety of from 70 to 120 amino acid residues and is derived from the C-terminal fragment of a spider silk protein. The fusion protein is not comprising any moiety derived from the repetitive fragment of a spider silk protein.