公开(公告)号：US20230174956A1

公开(公告)日：2023-06-08

申请号：US17926369

申请日：2021-05-25

Applicant: Spiber Inc.

Inventor： David Lips ,  Gijs Verkleij

IPC: C12N9/10 ,  C12N15/10

CPC classification number: C12N9/1029 ,  C12N15/1068 ,  C12N15/1051 ,  C12Y203/01

Abstract: The present invention relates to a method of preparing a gene cluster construct including a plurality of genes encoding a multi-modular biosynthetic enzyme, the method including (A) a step of preparing a plurality of DNA fragments which are capable of reconstructing the gene cluster construct and have structures that can be ligated to each other and (B) a step of ligating the plurality of DNA fragments prepared in the step (A) to each other by mixing the plurality of DNA fragments in a solution to obtain the gene cluster construct.

公开(公告)号：US20220411968A1

公开(公告)日：2022-12-29

申请号：US17764146

申请日：2020-09-28

Applicant: Spiber Inc.

Inventor： Hiroaki Suzumura ,  Yui Hirose

IPC: D01F4/02 ,  C07K14/435

Abstract: An object of the present invention is to provide an agent for imparting a cool touch feeling property and a water-absorbing and quick-drying property. The agent for imparting a cool touch feeling property and a water-absorbing and quick-drying property contains a modified fibroin as an active ingredient.

公开(公告)号：US20220119463A1

公开(公告)日：2022-04-21

申请号：US17421243

申请日：2020-01-09

Applicant: Spiber Inc.

Inventor： Keisuke MORITA ,  Yunosuke ABE ,  Hiroyuki NAKAMURA ,  Shota TOGASHI ,  Tetsuo ASAKURA

IPC: C07K14/435 ,  C12N15/63 ,  D01F4/02 ,  C12P21/02

Abstract: The present invention relates to a modified fibroin including a domain sequence represented by Formula 1: [(A)n motif-REP]m or Formula 2: [(A)n motif-REP]m-(A)n motif, and having a serine residue content rate of less than 5.5%.
[In Formula 1 and Formula 2, the (A)n motif represents an amino acid sequence consisting of 4 to 27 amino acid residues, and the number of alanine residues with respect to the total number of amino acid residues in the (A)n motif is 80% or more. REP represents an amino acid sequence consisting of 10 to 200 amino acid residues. m represents an integer of 10 to 300. The plurality of (A)n motifs may be the same amino acid sequence or different amino acid sequences. A plurality of REPs may be the same amino acid sequence or different amino acid sequences.]

公开(公告)号：US20220056221A1

公开(公告)日：2022-02-24

申请号：US17274557

申请日：2019-09-13

Applicant: Spiber Inc.

Inventor： Hisahiro Kudo ,  Hideto Ishii ,  Tatsuki Adachi ,  Ryoji Okada

IPC: C08J3/09 ,  C07K14/435 ,  D01D1/02 ,  D01F4/02

Abstract: Disclosed is a method for manufacturing a fibroin solution including a step of forming a slurry containing a solvent-containing dissolving liquid and a fibroin-containing protein powder dispersed in the dissolving liquid by continuously introducing the protein powder to a thin film of the dissolving liquid while flowing the thin film; and a step of forming a fibroin solution by dissolving, in the dissolving liquid, the protein powder in the slurry.

公开(公告)号：US20210388557A1

公开(公告)日：2021-12-16

申请号：US16491655

申请日：2018-03-08

Applicant: SPIBER INC. ,  KOJIMA INDUSTRIES CORPORATION

Inventor： Masataka KAJI ,  Ryuhei ENDO ,  Satoshi MiYAGUCHI ,  Kohsuke TOGASHI ,  Daichi YAMAGAMI ,  Mizuki IGARASHI ,  Takahito NISHIKADO

IPC: D06M11/05

Abstract: The present invention relates to a method for producing a protein fiber, including a step of bringing a protein raw fiber containing a protein into contact with water vapor in a storing chamber in which a temperature is adjusted within a range of less than 120° C. to perform heat treatment of the protein raw fiber.

公开(公告)号：US20210340193A1

公开(公告)日：2021-11-04

申请号：US17280125

申请日：2019-09-30

Applicant: Spiber Inc.

Inventor： Tetsuo Asakura ,  Yunosuke Abe ,  Hideto Ishii

IPC: C07K14/435 ,  D01F4/02

Abstract: A production method of a protein composition, the method comprising a step of hydrolyzing an ester group by bringing a raw material composition containing an esterified protein into contact with an acidic or basic medium.

公开(公告)号：US11117937B2

公开(公告)日：2021-09-14

申请号：US15989400

申请日：2018-05-25

Applicant: AJINOMOTO CO., INC. ,  SPIBER INC.

Inventor： Kentaro Nakase ,  Yoshinori Takashima ,  Takeshi Nagahiko

IPC: C12N15/09 ,  C12N15/71 ,  D01F4/02 ,  C07K14/435 ,  C12P21/02

Abstract: A method for producing a fibroin-like protein is provided. A fibroin-like protein is produced by a method of culturing Escherichia coli having a gene encoding the fibroin-like protein in a medium, inducing expression of the gene encoding the fibroin-like protein, and collecting the fibroin-like protein, wherein accumulation of an organic acid at the time of inducing the expression is reduced, and wherein the gene is expressed under control of a tryptophan promoter.

公开(公告)号：US10961285B2

公开(公告)日：2021-03-30

申请号：US15760497

申请日：2016-09-08

Applicant: Spiber Inc. ,  Kojima Industries Corporation

Inventor： Junichi Sugahara ,  Kaori Sekiyama ,  Ayumi Abe ,  Junichi Shimokata ,  Junichi Noba ,  Shinji Hirai

IPC: C07K14/43 ,  B29C43/02 ,  C08H1/00 ,  B29C43/00 ,  C07K14/435

Abstract: Provided is a molded article of a composition comprising a polypeptide, wherein the polypeptide is at least one kind selected from the group consisting of natural spider silk protein and polypeptides derived from natural spider silk protein.

公开(公告)号：US20210040648A1

公开(公告)日：2021-02-11

申请号：US16965626

申请日：2019-01-31

Applicant: Spiber Inc.

Inventor： Yunosuke Abe ,  Hirotada Ando

IPC: D01F4/02 ,  D01D5/06

Abstract: The present invention relates to a method for manufacturing a protein fiber, the method including a step of bringing a spinning raw liquid containing a spider silk protein and a solvent into contact with a coagulation liquid to coagulate the spider silk protein, in which the coagulation liquid contains a ketone.

公开(公告)号：US20210032778A1

公开(公告)日：2021-02-04

申请号：US16966155

申请日：2019-01-31

Applicant: Spiber Inc.

Inventor： Takuya Saito ,  Ryoji Okada ,  Naoki Matsuzaka

IPC: D01F4/02 ,  C07K1/113 ,  C07K14/435

Abstract: The present invention relates to a production method for a protein molded article, including: dissolving a protein in a solvent containing formic acid at a temperature of 40° C. or higher and lower than 80° C. to obtain a protein solution; and molding a protein molded article using the protein solution.