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公开(公告)号：US11117937B2
公开(公告)日：2021-09-14
申请号：US15989400
申请日：2018-05-25
申请人： AJINOMOTO CO., INC. , SPIBER INC.
IPC分类号： C12N15/09 , C12N15/71 , D01F4/02 , C07K14/435 , C12P21/02
摘要： A method for producing a fibroin-like protein is provided. A fibroin-like protein is produced by a method of culturing Escherichia coli having a gene encoding the fibroin-like protein in a medium, inducing expression of the gene encoding the fibroin-like protein, and collecting the fibroin-like protein, wherein accumulation of an organic acid at the time of inducing the expression is reduced, and wherein the gene is expressed under control of a tryptophan promoter.
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公开(公告)号：US20180265555A1
公开(公告)日：2018-09-20
申请号：US15989400
申请日：2018-05-25
申请人： AJINOMOTO CO., INC. , SPIBER INC.
IPC分类号： C07K14/435 , C12N15/71 , C12P21/02
CPC分类号： C07K14/43518 , C07K2319/02 , C07K2319/034 , C07K2319/035 , C12N15/09 , C12N15/71 , C12P21/02 , D01F4/02
摘要： A method for producing a fibroin-like protein is provided. A fibroin-like protein is produced by a method of culturing Escherichia coli having a gene encoding the fibroin-like protein in a medium, inducing expression of the gene encoding the fibroin-like protein, and collecting the fibroin-like protein, wherein accumulation of an organic acid at the time of inducing the expression is reduced, and wherein the gene is expressed under control of a tryptophan promoter.
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公开(公告)号：US20190016763A1
公开(公告)日：2019-01-17
申请号：US16144562
申请日：2018-09-27
申请人： AJINOMOTO CO., INC.
发明人： Manabu Kitazawa , Nao Sugimoto , Riho Kodama , Takeshi Nagahiko , Yoshihiro Ito , Kentaro Nakase , Yoshinori Takashima , Ayako Mine , Yoko Kuwabara , Uno Tagami , Masayuki Sugiki
摘要： A technique for efficiently culturing cells such as stem cells is provided. Cells such as stem cells are cultured using a cell culture vessel coated with a fibroin-like protein into which a cell adhesion sequence containing RGD (Arg-Gly-Asp) is inserted.
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公开(公告)号：US20230416793A1
公开(公告)日：2023-12-28
申请号：US18307947
申请日：2023-04-27
申请人： Ajinomoto Co., Inc.
发明人： Seiko Hirano , Kota Inoue , Kazuyuki Hayashi , Kosuke Yokota , Mika Moriya , Tomoko Suzuki , Yoshitomo Kadokura , Takeshi Nagahiko , Akari Tashiro
CPC分类号： C12P13/14 , C12N15/77 , C12N9/1217 , C12N9/16 , C12N9/0008 , C12N9/1096 , C12N9/0006 , C12N9/88 , C12R2001/15
摘要： Provided is a method of producing an L-amino acid such as L-glutamic acid and the like. An L-amino-acid is produced by cultivating a coryneform bacterium having L-amino acid-producing ability in a culture medium, which has been modified so as to have one or more of the following modifications: (A) a modification for increasing activity of acetate kinase, (B) a modification for increasing activity of fructose-1,6-bisphosphatase, (C) a modification for decreasing activity of pyruvate dehydrogenase, (D) a modification for decreasing activity of aspartate transaminase, and (E) a modification for decreasing activity of malic enzyme; and collecting the L-amino acid from the culture medium and/or the bacterial cells.
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