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公开(公告)号：US06822144B1
公开(公告)日：2004-11-23
申请号：US08963096
申请日：1997-11-03
Applicant: Zuo-Yu Zhao , Weining Gu , Tishu Cai , Dorothy A. Pierce
Inventor： Zuo-Yu Zhao , Weining Gu , Tishu Cai , Dorothy A. Pierce
IPC: C12N1584
CPC classification number: C12N15/8205
Abstract: The present invention relates to methods for improving the transformation frequency of Agrobacterium-mediated transformation of maize embryos. A preferred method for transforming maize using Agrobacterium comprises the steps of: contacting at least one immature embryo from a maize plant with Agrobacterium capable of transferring at least one gene to said embryo; co-cultivating the embryos with Agrobacterium; culturing the embryos in medium comprising N6 salts, an antibiotic capable of inhibiting the growth of Agrobacterium, and a selective agent to select for embryos expressing the gene; and regenerating plants expressing the gene.
Abstract translation: 本发明涉及改善农杆菌介导的玉米胚转化的转化频率的方法。 使用农杆菌转化玉米的优选方法包括以下步骤：使来自玉米植物的至少一种未成熟胚与能够将至少一种基因转移至所述胚的农杆菌接触; 与土壤杆菌共培养胚胎; 在包含N6盐的培养基中培养胚胎，能够抑制农杆菌生长的抗生素，以及选择表达基因的胚胎的选择剂; 和再生表达该基因的植物。
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公开(公告)号：US20130055472A1
公开(公告)日：2013-02-28
申请号：US13599003
申请日：2012-08-30
Applicant: Myeong-Je Cho , Theodore M. Klein , Zuo-Yu Zhao
Inventor： Myeong-Je Cho , Theodore M. Klein , Zuo-Yu Zhao
CPC classification number: C12N15/8205 , A01H4/00
Abstract: Compositions and methods for the efficient transformation and regeneration of monocot plants are provided. The methods of transformation involve infection with Agrobacterium. In this manner, any gene of interest can be introduced into the monocot plant with high transformation efficiency and in low copy number. Transformed and regenerated monocot cells, tissues, plants, and seed are also provided. The invention encompasses regenerating transformed plants, transgenic seeds produced therefrom, and transgenic plants and transgenic seeds from subsequent generations.
Abstract translation: 提供单叶植物有效转化和再生的组合物和方法。 转化方法涉及土壤杆菌感染。 以这种方式，任何感兴趣的基因可以以高转化效率和低拷贝数引入单子叶植物。 还提供了转化和再生的单子叶植物细胞，组织，植物和种子。 本发明包括再生转化的植物，由其产生的转基因种子，以及来自后代的转基因植物和转基因种子。
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23.发明申请DIRECT AND CONTINUOUS ROOT ALONE OR ROOT/SHOOT PRODUCTION FROM TRANSGENIC EVENTS DERIVED FROM GREEN REGENERATIVE TISSUES AND ITS APPLICATIONS 审中-公开从绿色再生组织衍生的转基因事件及其应用中直接和连续的根源或根/生产公开(公告)号：US20110165561A1
公开(公告)日：2011-07-07
申请号：US12977715
申请日：2010-12-23
Applicant: Myeong-Je Cho , David C. Cerf , Deping Xu , Zuo-Yu Zhao
Inventor： Myeong-Je Cho , David C. Cerf , Deping Xu , Zuo-Yu Zhao
CPC classification number: C12N15/821 , A01G22/00 , C12N15/8201 , C12N15/8227 , C12N15/8286 , G01N33/5097 , G01N2333/415 , G01N2333/43552 , Y02A40/162
Abstract: The present invention provides assays and methods for efficiently testing a polynucleotide of interest for a phenotype in a root. In some embodiments, the assays and methods include regenerating green tissue that is transgenic for at least one polynucleotide of interest into one or more transgenic plantlets that have at least one transgenic root. Further provided are methods of making a root assay by contacting green tissue with a first rooting medium to produce a plantlet and a plurality of roots. Additionally provided are methods of assaying for insecticidal activity on a live root. Accordingly provided herein is a substantially contamination-free, root bioassay. Further provided are methods of identifying a promoter having activity in a root.
Abstract translation: 本发明提供了用于有效测试根中表型的目的多核苷酸的测定和方法。 在一些实施方案中，测定和方法包括将至少一种感兴趣的多核苷酸转基因的绿色组织再生成具有至少一个转基因根的一个或多个转基因植物。 还提供了通过使绿色组织与第一生根培养基接触以产生小植物和多个根来进行根分析的方法。 另外提供了测定活根的杀虫活性的方法。 因此，本文提供了基本上无污染的根生物测定。 还提供了鉴定在根中具有活性的启动子的方法。
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公开(公告)号：US20100017908A1
公开(公告)日：2010-01-21
申请号：US12174812
申请日：2008-07-17
Applicant: Zuo-Yu Zhao , Ning Wang , Douglas S. Nubel , Shifu Zhen
Inventor： Zuo-Yu Zhao , Ning Wang , Douglas S. Nubel , Shifu Zhen
CPC classification number: A01H5/10
Abstract: A novel double haploid maize line designated PH17AW and seed, plants and plant parts thereof. Methods for producing a maize plant that comprise crossing double haploid maize line PH17AW with another maize plant. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into PH17AW through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby. Hybrid maize seed, plant or plant part produced by crossing the double haploid line PH17AW or a trait conversion of PH17AW with another maize line. Inbred maize lines derived from double haploid maize line PH17AW, methods for producing other inbred maize lines derived from double haploid maize line PH17AW and the inbred maize lines and their parts derived by those methods.
Abstract translation: 一种新型双倍体玉米品系，命名为PH17AW，其种子，植物和植物部分。 用于生产玉米植物的方法，其包括用另一种玉米植物杂交双倍单倍体玉米品系PH17AW。 用于生产含有其遗传物质的玉米植物的方法通过回交转化和/或转化渗入PH17AW的一种或多种性状，以及由此产生的玉米种子，植物和植物部分。 杂交玉米种子，植物或植物部分通过双重单倍体系PH17AW或PH17AW与另一个玉米品系的转化而产生。 衍生自双倍体单倍体玉米品系PH17AW的近交玉米品系，从双倍体单倍体玉米品系PH17AW生产其他近交玉米品系的方法和由这些方法衍生的近交玉米品系及其部分。
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公开(公告)号：US20090288217A1
公开(公告)日：2009-11-19
申请号：US12509538
申请日：2009-07-27
Applicant: Zuo-Yu Zhao , Ning Wang
Inventor： Zuo-Yu Zhao , Ning Wang
CPC classification number: A01H5/10
Abstract: A novel double haploid maize line designated PHWVZ and seed, plants and plant parts thereof. Methods for producing a maize plant that comprise crossing double haploid maize line PHWVZ with another maize plant. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into PHWVZ through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby. Hybrid maize seed, plant or plant part produced by crossing the double haploid line PHWVZ or a trait conversion of PHWVZ with another maize line. Inbred maize lines derived from double haploid maize line PHWVZ, methods for producing other inbred maize lines derived from double haploid maize line PHWVZ and the inbred maize lines and their parts derived by the use of those method.
Abstract translation: 一种新型双倍体玉米品系，命名为PHWVZ及其种子，植物和植物部分。 用于生产玉米植物的方法，其包括用另一种玉米植物杂交双倍体单倍体玉米品系PHWVZ。 用于生产含有其遗传物质的玉米植物的方法通过回交转化和/或转化渗透到PHWVZ中的一种或多种性状，以及由此产生的玉米种子，植物和植物部分。 杂交玉米种子，植物或植物部分通过双重单倍体系PHWVZ或PHWVZ与另一个玉米品系的转化而产生。 来自双倍体单倍体玉米品系PHWVZ的近交玉米品系，由双倍体单倍体玉米品系PHWVZ和近交玉米品系及其部分产生的其他近交玉米品系的生产方法。
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Patent Agency Ranking
公开(公告)号：US20080244765A1
公开(公告)日：2008-10-02
申请号：US12062212
申请日：2008-04-03
Applicant: Zuo-Yu Zhao , Youngzhong Wu , Michael Lassner , Howard Hershey
Inventor： Zuo-Yu Zhao , Youngzhong Wu , Michael Lassner , Howard Hershey
CPC classification number: C12N15/8289 , C07K14/415 , C12N15/8216
Abstract: Pollination process is disrupted by use of a pollination-disruption polynucleotide that renders the pollen unable to fertilize a sexually compatible ovule. The non-lethal nature of the pollen disruption polynucleotide is advantageous, particularly when operably linked to a transgenic polynucleotide of interest and prevents transmission of the polynucleotide through pollen. non-lethal markers are employed in an embodiment in which transgenic and non-transgenic seed can be sorted. A recombinase excision system is employed in an embodiment to activate the pollen disruption polynucleotide.
Abstract translation: 通过使用授粉破坏多核苷酸破坏授粉过程，使得花粉不能施用性别相容的胚珠。 花粉破坏多核苷酸的非致死性质是有利的，特别是当与感兴趣的转基因多核苷酸可操作地连接并且防止多核苷酸通过花粉传播时。 在其中可以分选转基因和非转基因种子的实施方案中采用非致死性标记。 在一个实施方案中采用重组酶切除系统来活化花粉破坏多核苷酸。
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公开(公告)号：US20080072344A1
公开(公告)日：2008-03-20
申请号：US11531801
申请日：2006-09-14
Applicant: Ning Wang , Lynn L. Heetland , William E. Solawetz , Zuo-Yu Zhao
Inventor： Ning Wang , Lynn L. Heetland , William E. Solawetz , Zuo-Yu Zhao
CPC classification number: A01H5/10
Abstract: A novel inbred maize line designated PHWWE and seed, plants and plant parts thereof. Methods for producing a maize plant that comprise crossing inbred maize line PHWWE with another maize plant. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into PHWWE through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby. Hybrid maize seed, plant or plant part produced by crossing the inbred line PHWWE or a trait conversion of PHWWE with another maize line. Inbred maize lines derived from maize line PHWWE, methods for producing other inbred maize lines derived from inbred maize line PHWWE and the inbred maize lines and their parts derived by the use of those methods.
Abstract translation: 一种新型近交玉米品系被指定为PHWWE及其种子，植物和植物部分。 用于生产玉米植物的方法，其包括用另一玉米植物杂交近交玉米品系PHWWE。 用于生产含有其遗传物质的玉米植物的方法通过回交转化和/或转化渗透到PHWWE中的一种或多种性状，以及由此产生的玉米种子，植物和植物部分的方法。 杂交玉米种子，植物或植物部分通过交叉自交系PHWWE或PHWWE与另一种玉米品系的转化而产生。 衍生自玉米系PHWWE的近交玉米品系，由近交玉米系PHWWE生产其他近交玉米品系的方法以及通过使用这些方法得到的近交玉米品系及其成分。
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公开(公告)号：US20070220627A1
公开(公告)日：2007-09-20
申请号：US11559022
申请日：2006-11-13
Applicant: Margit C. Ross , Zuo-yu Zhao , Dennis L. Bidney , Drew L. Brumbaugh
Inventor： Margit C. Ross , Zuo-yu Zhao , Dennis L. Bidney , Drew L. Brumbaugh
IPC: A01H5/00
CPC classification number: C12N15/8209
Abstract: Compositions and methods are provided to screen, identify, select, isolate, and/or regenerate targeted integration events using seed priming. Seed priming provides the identification of a seed having stably incorporated into its genome a site-specific recombinase mediated integration of a selectable marker at a target locus operably linked to a promoter active in the seed.
Abstract translation: 提供组合和方法以使用种子启动来筛选，识别，选择，分离和/或再生目标整合事件。 种子引发提供了稳定地并入其基因组中的种子的鉴定，其位点特异性重组酶介导的可选择标记在可操作地连接到在种子中活化的启动子的靶基因座上的整合。
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公开(公告)号：US20070143880A1
公开(公告)日：2007-06-21
申请号：US11427371
申请日：2006-06-29
Applicant: Dennis L. Bidney , Sheila E. Maddock , Xinli E. Wu , Zuo-Yu Zhao
Inventor： Dennis L. Bidney , Sheila E. Maddock , Xinli E. Wu , Zuo-Yu Zhao
CPC classification number: C12N15/8205 , C12N15/8207 , C12N15/8213
Abstract: The present invention provides methods and compositions which deliver Agrobacterium via microinjection directly into the embryo sac. At the time of injection, the embryo sac can comprise an egg cell, or alternatively, the embryo sac can be fertilized and comprise either a zygote or an embryo. Once inside the embryo sac, the Agrobacterium harboring a T-DNA having a polynucleotide of interest can express of the polynucleotide of interest in the plant. Further, the Agrobacterium can transfer the T-DNA having the polynucleotide of interest to the plant nucleus to produce a transformed plant. The polynucleotide of interest may be stably integrated into the genome of the egg cell, zygote, embryo, or endosperm, and any tissue, plant part, and/or plant generated therefrom.
Abstract translation: 本发明提供了通过显微注射将土壤杆菌直接递送到胚囊中的方法和组合物。 在注射时，胚囊可以包括卵细胞，或者，胚囊可被受精并且包括受精卵或胚胎。 一旦进入胚囊，携带具有感兴趣的多核苷酸的T-DNA的农杆菌可以在植物中表达感兴趣的多核苷酸。 此外，农杆菌可以将具有目标多核苷酸的T-DNA转移到植物核，以产生转化的植物。 感兴趣的多核苷酸可以稳定地整合到卵细胞，合子，胚或胚乳的基因组中，以及由其产生的任何组织，植物部分和/或植物。
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公开(公告)号：US20060101540A1
公开(公告)日：2006-05-11
申请号：US11280890
申请日：2005-11-16
Applicant: Jerome Ranch , Wallace Marsh , Dwight Tomes , Zuo-Yu Zhao
Inventor： Jerome Ranch , Wallace Marsh , Dwight Tomes , Zuo-Yu Zhao
CPC classification number: C12N15/8202 , A01H1/04 , A01H5/10 , C12N15/8201 , C12N15/8205 , C12N15/8207 , C12N15/8209 , C12Q1/6855 , C12Q1/6895 , C12Q2600/156
Abstract: Methods for plant transformation, for improving transformation efficiency, and for producing transgenic plants are provided. The methods comprise crossing a recipient plant from a genetic line of a plant species of interest with a donor plant selected from a transformation competent genetic line of the same plant species or of another closely related plant species to obtain a hybrid plant. Tissues obtained from the hybrid plant are transformation competent. These tissues can then be transformed with one or more nucleotide sequences of interest and selected for transgenic events having the nucleotide sequence of interest integrated within a chromosome derived from the recipient plant. Transformed cells can be selected and transgenic hybrid plants regenerated. The nucleotide sequence of interest can be introgressed into the genetic line from which the original recipient parent was derived, or into other genetic lines. Transformed plants and seeds are additionally provided.
Abstract translation: 提供了用于植物转化，提高转化效率和用于产生转基因植物的方法。 所述方法包括将受感染植物从感兴趣的植物物种的遗传系与选自相同植物物种的转基因遗传系或另一密切相关的植物物种的供体植物杂交以获得杂种植物。 从混合植物获得的组织是转化能力。 然后可以用一个或多个感兴趣的核苷酸序列转化这些组织，并选择用于整合在来自受体植物的染色体内的感兴趣的核苷酸序列的转基因事件。 可以选择转化的细胞，并再生转基因杂交植物。 感兴趣的核苷酸序列可以渗入原始受体亲本来源的基因系，或其他遗传系。 另外提供转化的植物和种子。
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