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    Methods for tissue culture and transformation of sugarcane
    1.
    发明申请
    Methods for tissue culture and transformation of sugarcane 审中-公开
    甘蔗组织培养和转化方法

    公开(公告)号：US20130055472A1

    公开(公告)日：2013-02-28

    申请号：US13599003

    申请日：2012-08-30

    申请人： Myeong-Je Cho ,  Theodore M. Klein ,  Zuo-Yu Zhao

    发明人： Myeong-Je Cho ,  Theodore M. Klein ,  Zuo-Yu Zhao

    IPC分类号： A01H1/06 ,  C12N5/04

    CPC分类号： C12N15/8205 ,  A01H4/00

    摘要： Compositions and methods for the efficient transformation and regeneration of monocot plants are provided. The methods of transformation involve infection with Agrobacterium. In this manner, any gene of interest can be introduced into the monocot plant with high transformation efficiency and in low copy number. Transformed and regenerated monocot cells, tissues, plants, and seed are also provided. The invention encompasses regenerating transformed plants, transgenic seeds produced therefrom, and transgenic plants and transgenic seeds from subsequent generations.

    摘要翻译： 提供单叶植物有效转化和再生的组合物和方法。 转化方法涉及土壤杆菌感染。 以这种方式，任何感兴趣的基因可以以高转化效率和低拷贝数引入单子叶植物。 还提供了转化和再生的单子叶植物细胞，组织，植物和种子。 本发明包括再生转化的植物，由其产生的转基因种子，以及来自后代的转基因植物和转基因种子。

    DIRECT AND CONTINUOUS ROOT ALONE OR ROOT/SHOOT PRODUCTION FROM TRANSGENIC EVENTS DERIVED FROM GREEN REGENERATIVE TISSUES AND ITS APPLICATIONS
    2.
    发明申请
    DIRECT AND CONTINUOUS ROOT ALONE OR ROOT/SHOOT PRODUCTION FROM TRANSGENIC EVENTS DERIVED FROM GREEN REGENERATIVE TISSUES AND ITS APPLICATIONS 审中-公开
    从绿色再生组织衍生的转基因事件及其应用中直接和连续的根源或根/生产

    公开(公告)号：US20110165561A1

    公开(公告)日：2011-07-07

    申请号：US12977715

    申请日：2010-12-23

    申请人： Myeong-Je Cho ,  David C. Cerf ,  Deping Xu ,  Zuo-Yu Zhao

    发明人： Myeong-Je Cho ,  David C. Cerf ,  Deping Xu ,  Zuo-Yu Zhao

    IPC分类号： C12Q1/68 ,  C12Q1/18 ,  C12Q1/02 ,  A01G1/00

    CPC分类号： C12N15/821 ,  A01G22/00 ,  C12N15/8201 ,  C12N15/8227 ,  C12N15/8286 ,  G01N33/5097 ,  G01N2333/415 ,  G01N2333/43552 ,  Y02A40/162

    摘要： The present invention provides assays and methods for efficiently testing a polynucleotide of interest for a phenotype in a root. In some embodiments, the assays and methods include regenerating green tissue that is transgenic for at least one polynucleotide of interest into one or more transgenic plantlets that have at least one transgenic root. Further provided are methods of making a root assay by contacting green tissue with a first rooting medium to produce a plantlet and a plurality of roots. Additionally provided are methods of assaying for insecticidal activity on a live root. Accordingly provided herein is a substantially contamination-free, root bioassay. Further provided are methods of identifying a promoter having activity in a root.

    摘要翻译： 本发明提供了用于有效测试根中表型的目的多核苷酸的测定和方法。 在一些实施方案中，测定和方法包括将至少一种感兴趣的多核苷酸转基因的绿色组织再生成具有至少一个转基因根的一个或多个转基因植物。 还提供了通过使绿色组织与第一生根培养基接触以产生小植物和多个根来进行根分析的方法。 另外提供了测定活根的杀虫活性的方法。 因此，本文提供了基本上无污染的根生物测定。 还提供了鉴定在根中具有活性的启动子的方法。

    Methods for improving monocot transformation
    3.
    发明授权
    Methods for improving monocot transformation 失效
    改善单子叶植物转化的方法

    公开(公告)号：US08404930B2

    公开(公告)日：2013-03-26

    申请号：US12694013

    申请日：2010-01-26

    申请人： Xinli E. Wu ,  Myeong-Je Cho ,  Zuo-Yu Zhao

    发明人： Xinli E. Wu ,  Myeong-Je Cho ,  Zuo-Yu Zhao

    IPC分类号： C12N15/84 ,  A01H4/00

    CPC分类号： C12N15/8205

    摘要： The invention provides improved plant transformation methods. In particular the method provides increased transformation frequency, especially in recalcitrant plants. The method includes various transformation protocols for monocots, such as maize and sorghum, using a combination of media and light conditions to achieve increased efficiency of monocot transformation and increased callus initiation frequencies.

    摘要翻译： 本发明提供改进的植物转化方法。 特别地，该方法提供增加的转化频率，特别是在顽固植物中。 该方法包括用于单子叶植物（例如玉米和高粱）的各种转化方案，其使用培养基和光照条件的组合来实现提高单子叶植物转化的效率和增加的愈伤组织起始频率。

    Compositions and methods for plant transformation and regeneration
    5.
    发明授权
    Compositions and methods for plant transformation and regeneration 失效
    植物转化和再生的组成和方法

    公开(公告)号：US06235529B1

    公开(公告)日：2001-05-22

    申请号：US08845939

    申请日：1997-04-29

    申请人： Peggy G. Lemaux ,  Myeong-Je Cho

    发明人： Peggy G. Lemaux ,  Myeong-Je Cho

    IPC分类号： C12N504

    CPC分类号： A01H4/005

    摘要： Improved compositions and methods for transformation and regeneration of plants from embryogenic callus are disclosed that include, for example: use of an intermediate-incubation medium after callus induction to increase the competence of the transformed cells for regeneration; dim light conditions during early phases of selection; use of green callus tissue as a target for microprojectile bombardment; and media with optimized levels of phytohormones and copper concentrations.

    摘要翻译： 公开了用于从胚性愈伤组织转化和再生植物的改进的组合物和方法，其包括例如：在愈伤组织诱导后使用中间培养培养基以增加转化细胞的再生能力; 选择早期阶段的昏暗条件; 使用绿色愈伤组织作为微粒轰击的目标; 和具有优化水平的植物激素和铜浓度的培养基。

    Transgenic plants with elevated thioredoxin levels
    10.
    发明授权
    Transgenic plants with elevated thioredoxin levels 失效
    具有升高的硫氧还蛋白水平的转基因植物

    公开(公告)号：US07179964B2

    公开(公告)日：2007-02-20

    申请号：US10194885

    申请日：2002-07-12

    申请人： Myeong-Je Cho ,  Joshua H. Wong ,  Peggy G. Lemaux ,  Bob B. Buchanan

    发明人： Myeong-Je Cho ,  Joshua H. Wong ,  Peggy G. Lemaux ,  Bob B. Buchanan

    IPC分类号： C12N15/82 ,  A01H5/00

    CPC分类号： A21D13/064 ,  A23J1/12 ,  A23K10/30 ,  A23L33/105 ,  A23V2002/00 ,  C07K14/415 ,  C12N9/0036 ,  C12N15/8242 ,  C12N15/8245 ,  C12N15/8251 ,  C12N15/8261 ,  Y02A40/146 ,  A23V2300/21

    摘要： The present invention is directed to a transgenic plant wherein at least a part of said plant includes a recombinant nucleic acid with a promoter active in the part operably linked to a nucleic acid encoding a thioredoxin polypeptide wherein the promoter is a seed or grain maturation-specific promoter and the thioredoxin polypeptide includes the amino acid sequence WCGPC. The present invention is further directed to transgenic plants that overexpress thioredoxin in seed wherein the overexpression of thioredoxin h effects a significant increase in the reduction of proteins (—SH as compared to S—S) of the albumin fraction of the seed.

    摘要翻译： 本发明涉及一种转基因植物，其中所述植物的至少一部分包括具有启动子活性的启动子的重组核酸，其与编码硫氧还蛋白多肽的核酸可操作地连接，其中所述启动子是种子或谷物成熟特异性 启动子和硫氧还蛋白多肽包括氨基酸序列WCGPC。 本发明进一步涉及在种子中过表达硫氧还蛋白的转基因植物，其中硫氧还蛋白的过表达h与种子的白蛋白部分的蛋白质的降低（-SH）相比显着增加。