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    ACYL-COA DEHYDROGENASE ASSAYS
    22.
    发明申请
    ACYL-COA DEHYDROGENASE ASSAYS 审中-公开
    ACYL-COA脱氢酶测定

    公开(公告)号：US20150267242A1

    公开(公告)日：2015-09-24

    申请号：US14439255

    申请日：2013-10-30

    Applicant: ADVANCED LIQUID LOGIC, INC

    Inventor： Nora J. Foegeding ,  Carrie A. Graham ,  Allen E. Eckhardt

    IPC: C12Q1/32

    CPC classification number: C12Q1/32 ,  G01N33/6893 ,  G01N2333/90206 ,  G01N2800/04 ,  G01N2800/7085

    Abstract: A method for conducting medium-chain acyl-CoA dehydrogenase (MCAD) and very-long-chain acyl-CoA dehydrogenase (VCAD) enzymatic activity assays is provided. The method may include, but is not limited to, preparing a sample; preparing an enzyme-specific substrate/reagent mixture; mixing an aliquot of the prepared sample with an aliquot of the enzyme-specific substrate/reagent mixture; reading absorbance in the range of about 600 nm; incubating the prepared sample and enzyme-specific substrate/reagent mixture; and reading absorbance in the range of about 600 nm at various time intervals.

    Abstract translation: 提供了一种用于进行中链酰基辅酶A脱氢酶（MCAD）和非常长链的酰基辅酶A脱氢酶（VCAD）酶活性测定的方法。 该方法可以包括但不限于制备样品; 制备酶特异性底物/试剂混合物; 将制备的样品的等分试样与酶特异性底物/试剂混合物的等分试样混合; 读取吸光度在约600nm范围内; 孵育制备的样品和酶特异性底物/试剂混合物; 并以各种时间间隔读取约600nm范围内的吸光度。

    Systems for and Methods of Hybrid Pyrosequencing
    24.
    发明申请
    Systems for and Methods of Hybrid Pyrosequencing 审中-公开
    混合焦磷酸测序系统和方法

    公开(公告)号：US20150065360A1

    公开(公告)日：2015-03-05

    申请号：US14538379

    申请日：2014-11-11

    Applicant: Advanced Liquid Logic, Inc.

    Inventor： Allen E. Eckhardt ,  Jonathan Benton ,  Deborah Boles

    IPC: C12N15/10 ,  C12Q1/70 ,  C12Q1/68

    CPC classification number: C12N15/1089 ,  C12Q1/6869 ,  C12Q1/689 ,  C12Q1/701 ,  C12Q1/703 ,  G16B30/00 ,  G16B40/00 ,  C12Q2537/165 ,  C12Q2565/301

    Abstract: The systems and methods of the invention provide a guided approach to pyrosequencing (i.e., hybrid pyrosequencing). A de novo nucleic acid sequence may compared to a library of possible results and the next nucleotide to be dispensed is selected based on the comparison of the de novo sequence and the library of possible results. In another example, at least the first nucleotide to be dispensed is selected based on a query of a database(s) of non-sequence parameters (e.g., incidence of infection, diagnostic symptoms, sample source) and subsequent dispensations determined based on a comparison of the de novo sequence and the library of possible results (e.g., candidate sequences). The systems and methods of the invention may be performed using a droplet actuator.

    Abstract translation: 本发明的系统和方法提供了焦磷酸测序（即，杂化焦磷酸测序）的指导方法。 从头核酸序列可以与可能结果的文库进行比较，并且基于从头序列与可能结果库的比较来选择待分配的下一个核苷酸。 在另一个实例中，基于对非序列参数（例如，感染发生率，诊断症状，样本来源）的数据库的查询和基于比较确定的随后分配来选择至少要分配的第一核苷酸 的新生序列和可能结果的库（例如候选序列）。 本发明的系统和方法可以使用液滴致动器来执行。

    Method of Preparing a Nucleic Acid Library
    25.
    发明申请
    Method of Preparing a Nucleic Acid Library 审中-公开
    制备核酸文库的方法

    公开(公告)号：US20150038344A1

    公开(公告)日：2015-02-05

    申请号：US14520736

    申请日：2014-10-22

    Applicant: ADVANCED LIQUID LOGIC, INC.

    Inventor： Michael G. Pollack ,  Allen E. Eckhardt ,  Prasanna Thwar ,  Jeremy Rouse

    IPC: C12N15/10

    CPC classification number: C12N15/1075 ,  B01J19/0046 ,  C12N15/1006 ,  C40B40/08 ,  C40B50/06

    Abstract: A method of preparing a nucleic acid library in droplets in contact with oil, including: (a) blunt-ending nucleic acid fragments in a droplet in the oil to yield blunt-ended nucleic acid fragments; (b) phosphorylating the blunt-ended nucleic acid fragments in a droplet in the oil to yield phosphorylated nucleic acid fragments; coupling A-tails to the phosphorylated nucleic acid fragments in a droplet in the oil to yield A-tailed nucleic acid fragments; and (d) coupling nucleic acid adapters to the A-tailed nucleic acid fragments in a droplet in the oil to yield the nucleic acid library comprising adapter-ligated nucleic acid fragments.

    Abstract translation: 制备与油接触的液滴核酸文库的方法，包括：（a）在油中的液滴中的平端核酸片段以产生平端核酸片段; （b）使油中的液滴中的平端核酸片段磷酸化以产生磷酸化的核酸片段; 将A尾连接到油中的液滴中的磷酸化核酸片段以产生A尾核酸片段; 和（d）将核酸衔接子偶联到油中的液滴中的A尾核酸片段，以产生包含衔接子连接的核酸片段的核酸文库。

    GLUCOSE-6-PHOSPHATE DEHYDROGENASE ASSAYS
    26.
    发明申请
    GLUCOSE-6-PHOSPHATE DEHYDROGENASE ASSAYS 审中-公开
    葡萄糖-6-磷酸脱氢酶测定

    公开(公告)号：US20140335069A1

    公开(公告)日：2014-11-13

    申请号：US14359177

    申请日：2012-11-20

    Applicant: ADVANCED LIQUID LOGIC, INC.

    Inventor： Carrie A. Graham ,  Allen E. Eckhardt

    IPC: C12Q1/32

    CPC classification number: C12Q1/32 ,  G01N2333/904

    Abstract: Aspects of embodiments may include methods for automated enzymatic detection of glucose-6-phosphate dehydrogenase (G6PD) activity. Aspects of embodiments may include methods for enzymatic detection of G6PD activity in droplets in oil. Aspects of embodiments may include a system including a droplet actuator. Aspects of embodiments may include a treatment method.

    Abstract translation: 实施方案的各方面可以包括葡萄糖-6-磷酸脱氢酶（G6PD）活性的自动酶检测方法。 实施方案的各方面可以包括用于在油中的液滴中酶检测G6PD活性的方法。 实施例的方面可以包括包括液滴致动器的系统。 实施方案的各方面可以包括治疗方法。

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