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    Bead Manipulation Techniques
    2.
    发明申请
    Bead Manipulation Techniques 审中-公开
    珠操作技术

    公开(公告)号：US20160271623A1

    公开(公告)日：2016-09-22

    申请号：US15165883

    申请日：2016-05-26

    Applicant: ADVANCED LIQUID LOGIC, INC.

    Inventor： Vamsee K. Pamula ,  Prasanna Thwar ,  Arjun Sudarsan ,  Ramakrishna Sista

    IPC: B03C7/02 ,  B01L3/00

    CPC classification number: B03C7/026 ,  B01L3/50273 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2300/0887 ,  B01L2400/0424 ,  B01L2400/0427 ,  B03C1/01 ,  B03C1/288 ,  B03C5/00 ,  B03C5/005 ,  B03C5/026 ,  B03C2201/26 ,  G01N33/54326

    Abstract: The invention provides a method of redistributing magnetically responsive beads in a droplet. The method may include conducting on a droplet operations surface one or more droplet operations using the droplet without removing the magnetically responsive beads from the region of the magnetic field. The droplet operations may in some cases be electrode-mediated. The droplet operations may redistribute and/or circulate the magnetically responsive beads within the droplet. In some cases, the droplet may include a sample droplet may include a target analyte. The redistributing of the magnetically responsive beads may cause target analyte to bind to the magnetically responsive beads. In some cases, the droplet may include unbound substances in a wash buffer. The redistributing of the magnetically responsive beads causes unbound substances to be freed from interstices of an aggregated set or subset of the magnetically responsive beads.

    Abstract translation: 本发明提供了一种在液滴中重新分布磁响应珠的方法。 该方法可以包括在液滴操作表面上使用液滴进行一个或多个液滴操作，而不从磁场的区域移除磁响应珠。 在某些情况下，液滴操作可能是电极介导的。 液滴操作可以在液滴内重新分布和/或循环磁响应珠。 在一些情况下，液滴可以包括样品液滴可以包括靶分析物。 磁响应珠的重新分布可能导致目标分析物结合磁响应珠。 在一些情况下，液滴可以包括洗涤缓冲液中的未结合物质。 磁响应珠子的重新分布导致未结合物质从磁性响应珠粒的聚集或子集的空隙中释放出来。

    Method of Preparing a Nucleic Acid Library
    3.
    发明申请
    Method of Preparing a Nucleic Acid Library 审中-公开
    制备核酸文库的方法

    公开(公告)号：US20150038344A1

    公开(公告)日：2015-02-05

    申请号：US14520736

    申请日：2014-10-22

    Applicant: ADVANCED LIQUID LOGIC, INC.

    Inventor： Michael G. Pollack ,  Allen E. Eckhardt ,  Prasanna Thwar ,  Jeremy Rouse

    IPC: C12N15/10

    CPC classification number: C12N15/1075 ,  B01J19/0046 ,  C12N15/1006 ,  C40B40/08 ,  C40B50/06

    Abstract: A method of preparing a nucleic acid library in droplets in contact with oil, including: (a) blunt-ending nucleic acid fragments in a droplet in the oil to yield blunt-ended nucleic acid fragments; (b) phosphorylating the blunt-ended nucleic acid fragments in a droplet in the oil to yield phosphorylated nucleic acid fragments; coupling A-tails to the phosphorylated nucleic acid fragments in a droplet in the oil to yield A-tailed nucleic acid fragments; and (d) coupling nucleic acid adapters to the A-tailed nucleic acid fragments in a droplet in the oil to yield the nucleic acid library comprising adapter-ligated nucleic acid fragments.

    Abstract translation: 制备与油接触的液滴核酸文库的方法，包括：（a）在油中的液滴中的平端核酸片段以产生平端核酸片段; （b）使油中的液滴中的平端核酸片段磷酸化以产生磷酸化的核酸片段; 将A尾连接到油中的液滴中的磷酸化核酸片段以产生A尾核酸片段; 和（d）将核酸衔接子偶联到油中的液滴中的A尾核酸片段，以产生包含衔接子连接的核酸片段的核酸文库。

    Droplet Actuator Devices and Methods for Manipulating Beads
    5.
    发明申请
    Droplet Actuator Devices and Methods for Manipulating Beads 审中-公开
    液滴致动器装置和操作珠的方法

    公开(公告)号：US20150107995A1

    公开(公告)日：2015-04-23

    申请号：US14589314

    申请日：2015-01-05

    Applicant: ADVANCED LIQUID LOGIC, INC.

    Inventor： Ramakrishna Sista ,  Vamsee Pamula ,  Arjun Sudarsan ,  Vijay Srinivasan ,  Prasanna Thwar

    IPC: B01L3/00 ,  G01N27/447

    CPC classification number: B01L3/502792 ,  B01L3/502761 ,  B01L7/52 ,  B01L2200/0647 ,  B01L2300/0819 ,  B01L2300/0851 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/089 ,  B01L2300/161 ,  B01L2400/0427 ,  B01L2400/043

    Abstract: The invention provides droplet actuators and droplet actuator techniques. Among other things, the droplet actuators and methods are useful for manipulating beads on a droplet actuator, such as conducting droplet operations using bead-containing droplets on a droplet actuator. For example, beads may be manipulated on a droplet actuator in the context of executing a sample preparation protocol and/or an assay protocol. An output of the methods of the invention may be beads prepared for execution of an assay protocol. Another output of the methods of the invention may be results of an assay protocol executed using beads. Among the methods described herein are methods of concentrating beads in droplets, methods of washing beads, methods of suspending beads, methods of separating beads, methods of localizing beads within a droplet, methods of forming emulsions in which droplets include beads, methods of loading beads into a droplet operations gap of a droplet actuator, methods of organizing beads in a monolayer, and methods of capturing, trapping or restraining beads.

    Abstract translation: 本发明提供了液滴致动器和液滴致动器技术。 除其他之外，液滴致动器和方法可用于操纵液滴致动器上的珠，例如使用液滴致动器上的含珠液滴进行液滴操作。 例如，在执行样品制备方案和/或测定方案的上下文中，珠可以在液滴致动器上操作。 本发明方法的输出可以是准备用于执行测定方案的珠粒。 本发明方法的另一个输出可以是使用珠子执行的测定方案的结果。 在本文描述的方法中，将珠粒集中在液滴中的方法，洗涤珠粒的方法，悬浮珠粒的方法，分离珠粒的方法，在液滴内定位珠粒的方法，形成液滴包括珠子的乳液的方法，加载珠粒的方法 液滴致动器的液滴操作间隙，单层组织珠粒的方法，以及捕获，捕获或抑制珠粒的方法。

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