公开(公告)号：US20230243807A1

公开(公告)日：2023-08-03

申请号：US18178130

申请日：2023-03-03

Applicant: ARIZONA BOARD OF REGENTS ON behalf of ARIZONA STATE UNIVERSITY

Inventor： Stuart Lindsay ,  Peiming Zhang ,  Yanan Zhao

IPC: G01N27/327

CPC classification number: G01N27/3276

Abstract: A single molecule sensing or detecting device includes a first electrode and a second electrode separated from the first electrode by a gap. The first electrode and the second electrode have an opening formed therethrough. At least one of the first electrode and the second electrode is functionalized with a recognition molecule. The recognition molecule has an effective length LI and is configured to selectively bind to a target molecule having an effective length L2. The size of the gap is configured to be greater than L2, but less than or equal to the sum of LI and L2.

公开(公告)号：US11325987B2

公开(公告)日：2022-05-10

申请号：US16155972

申请日：2018-10-10

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Peiming Zhang ,  Xu Wang ,  Jong One Im ,  Stuart Lindsay

IPC: G01N33/48 ,  G01N33/50 ,  C08B37/00 ,  G06N20/10 ,  G16B40/20 ,  G06N3/08 ,  G16B40/10

Abstract: The present disclosure provides a method for identifying and quantifying sulfated glycosaminoglycans, including for example heparin, by passing a sample through nanopores. The glycosaminoglycans sample is measured in microliter quantities, at nanomolar concentrations with detection of impurities below 0.5%, and a dynamic range over five decades of magnitude with a trained machine learning algorithm.

公开(公告)号：US10444220B2

公开(公告)日：2019-10-15

申请号：US15712552

申请日：2017-09-22

Applicant: Arizona Board of Regents on behalf of Arizona State University

Inventor： Stuart Lindsay ,  Shuai Chang ,  Jin He ,  Peiming Zhang ,  Shuo Huang

IPC: G01N33/487 ,  C12Q1/6869 ,  G01N27/327

Abstract: The invention includes compositions, devices, and methods for analyzing a polymer and/or polymer unit. The polymer may be a homo or hetero-polymer such as DNA, RNA, a polysaccharide, or a peptide. The device includes electrodes that form a tunnel gap through which the polymer can pass. The electrodes are functionalized with a reagent attached thereto, and the reagent is capable of forming a transient bond to a polymer unit. When the transient bond forms between the reagent and the unit, a detectable signal is generated and used to analyze the polymer.

公开(公告)号：US10379102B2

公开(公告)日：2019-08-13

申请号：US15375901

申请日：2016-12-12

Applicant: ARIZONA BOARD OF REGENTS on behalf of ARIZONA STATE UNIVERSITY

Inventor： Stuart Lindsay ,  Peiming Zhang ,  Yanan Zhao

IPC: G01N27/327 ,  G01N33/487

Abstract: A single molecule sensing or detecting device includes a first electrode and a second electrode separated from the first electrode by a gap. The first electrode and the second electrode have an opening formed therethrough. At least one of the first electrode and the second electrode is functionalized with a recognition molecule. The recognition molecule has an effective length L1 and is configured to selectively bind to a target molecule having an effective length L2. The size of the gap is configured to be greater than L2, but less than or equal to the sum of L1 and L2.

公开(公告)号：US10287257B2

公开(公告)日：2019-05-14

申请号：US15308517

申请日：2015-05-07

Applicant: Arizona Board of Regents on behalf of Arizona State University

Inventor： Peiming Zhang ,  Stuart Lindsay ,  Saikat Manna ,  Subhadip Senapati

IPC: C07D249/04 ,  C07D249/06 ,  C07D491/052 ,  C07D487/04 ,  C07C247/04 ,  C07K14/00 ,  C12N15/115

Abstract: Some of the embodiments of the present disclosure relate to a compound of the formula, and methods of preparing and using same.

公开(公告)号：US09593372B2

公开(公告)日：2017-03-14

申请号：US14577715

申请日：2014-12-19

Applicant: ARIZONA BOARD OF REGENTS on behalf of ARIZONA STATE UNIVERSITY

Inventor： Stuart Lindsay ,  Peiming Zhang

IPC: G01N27/447 ,  C12Q1/68 ,  G01N33/487

CPC classification number: C07D235/24 ,  C12Q1/6869 ,  G01N27/447 ,  G01N27/44791 ,  G01N33/48721 ,  C12Q2565/631

Abstract: The present invention is directed to systems, devices and methods for identifying biopolymers, such as strands of DNA, as they pass through a constriction such as a carbon nanotube nanopore. More particularly, the invention is directed to such systems, devices and methods in which a newly translocated portion of the biopolymer forms a temporary electrical circuit between the nanotube nanopore and a second electrode, which may also be a nanotube. Further, the invention is directed to such systems, devices and methods in which the constriction is provided with a functionalized unit which, together with a newly translocated portion of the biopolymer, forms a temporary electrical circuit that can be used to characterize that portion of the biopolymer.

Abstract translation: 本发明涉及用于识别生物聚合物的系统，装置和方法，例如当DNA通过诸如碳纳米管纳米孔的收缩体时，DNA的链。 更具体地，本发明涉及这样的系统，装置和方法，其中生物聚合物的新易位部分在纳米管纳米孔和也可以是纳米管的第二电极之间形成临时电路。 此外，本发明涉及这样的系统，装置和方法，其中收缩部设置有功能化单元，其与生物聚合物的新易位部分一起形成临时电路，其可用于表征该部分的 生物聚合物。

公开(公告)号：US20150144506A1

公开(公告)日：2015-05-28

申请号：US14401343

申请日：2013-03-15

Applicant: ARIZONA BOARD OF REGENTS on behalf of ARIZONA STATE UNIVERSITY

Inventor： Stuart Lindsay ,  Peiming Zhang ,  Hao Liu

IPC: G01N27/26

CPC classification number: G01N27/26 ,  G01N33/54373 ,  G01N2400/10

Abstract: Embodiments of the present disclosure are directed to recognition tunneling methods, systems and devices for the detection of carbohydrates by measuring tunneling currents of sugars which give distinct electronic signals in a tunnel gap functionalized respectively with, for example, in some embodiments, 4(5)-(2-mercaptoethyl)-1H imideazole-2-carboxamide and 4-mercaptophenylboronic acid molecules on at least one, and preferably each electrode.

Abstract translation: 本公开的实施例涉及用于通过测量糖的隧道电流来检测碳水化合物的识别隧道方法，系统和装置，所述隧道电流在例如在一些实施例中分别功能化的隧道间隙中给出不同的电子信号，4（5） - （2-巯基乙基）-1H亚氨基咪唑-2-甲酰胺和4-巯基苯基硼酸分子在至少一个，优选每个电极上。